scholarly journals First Complete Genome Sequence of Pseudomonas aeruginosa (Schroeter 1872) Migula 1900 (DSM 50071 T ), Determined Using PacBio Single-Molecule Real-Time Technology

2015 ◽  
Vol 3 (4) ◽  
Author(s):  
Kazuma Nakano ◽  
Yasunobu Terabayashi ◽  
Akino Shiroma ◽  
Makiko Shimoji ◽  
Hinako Tamotsu ◽  
...  

The first complete genome sequence of the type strain Pseudomonas aeruginosa (Schroeter 1872) Migula 1900 (DSM 50071 T ) was determined in a single contig by PacBio RS II. The genome (6,317,050 bp, G+C content of 66.52%) contained 10 sets of >1,000-bp identical sequence pairs and 183 tandem repeats.

2015 ◽  
Vol 3 (5) ◽  
Author(s):  
Thomas Riedel ◽  
Boyke Bunk ◽  
Johannes Wittmann ◽  
Andrea Thürmer ◽  
Cathrin Spröer ◽  
...  

In this study, we sequenced the complete genome of the Clostridium difficile type strain DSM 1296 T . A combination of single-molecule real-time (SMRT) and Illumina sequencing technology revealed the presence of one chromosome and two extrachromosomal elements, the bacteriophage phiCDIF1296T and a putative plasmid-like structure harboring genes of another bacteriophage.


2016 ◽  
Vol 4 (5) ◽  
Author(s):  
Kazuma Nakano ◽  
Akino Shiroma ◽  
Hinako Tamotsu ◽  
Shun Ohki ◽  
Makiko Shimoji ◽  
...  

The first complete genome sequence of Lactobacillus curvatus was determined by PacBio RS II. The single circular chromosome (1,848,756 bp, G+C content of 42.1%) of L. curvatus FBA2, isolated from fermented vegetables, contained low G+C regions (26.9% minimum) and 43 sets of >1,000-bp identical sequence pairs. No plasmids were detected.


2017 ◽  
Vol 5 (16) ◽  
Author(s):  
Raffael C. Inglin ◽  
Leo Meile ◽  
Jochen Klumpp ◽  
Marc J. A. Stevens

ABSTRACT We present here the complete genome sequence of Lactobacillus plantarum RI-113, a strain isolated from salami, which was determined using single-molecule real-time sequencing.


2016 ◽  
Vol 4 (5) ◽  
Author(s):  
Xiao-Heng Song ◽  
Hong-Xi Chen ◽  
Wang-Shu Zhou ◽  
Jiang-Bo Wang ◽  
Ma-Feng Liu ◽  
...  

Mycobacterium avium is an important pathogenic bacterium in birds and has never, to our knowledge, reported to be isolated from domestic ducks. We present here the complete genome sequence of a virulent strain of Mycobacterium avium , isolated from domestic Pekin ducks for the first time, which was determined by PacBio single-molecule real-time technology.


2019 ◽  
Vol 8 (37) ◽  
Author(s):  
Lakshmi Chandrasekaran ◽  
Daniela I. Drautz-Moses ◽  
Akira Uchida ◽  
Rikky W. Purbojati ◽  
Anthony Wong ◽  
...  

Citricoccus sp. strain SGAir0253 was isolated from indoor air collected in Singapore. Its genome sequence was assembled using single-molecule real-time sequencing. It comprises one chromosome of 3.32 Mb and two plasmids of 137 kb and 99 kb. The genome consists of 2,950 protein-coding genes, 49 tRNAs, and 9 rRNAs.


2017 ◽  
Vol 5 (35) ◽  
Author(s):  
Luis F. Espinosa-Camacho ◽  
Gabriela Delgado ◽  
Guadalupe Miranda-Novales ◽  
Gloria Soberón-Chávez ◽  
Luis D. Alcaraz ◽  
...  

ABSTRACT Two Pseudomonas aeruginosa strains isolated from children with bacteremia in Mexico City were sequenced using PacBio RS-II single-molecule real-time (SMRT) technology. The strains consist of a 7.0- to 7.4-Mb chromosome, with a high content of mobile elements, and variation in the genetic content of class 1 integron In1409.


2018 ◽  
Vol 6 (6) ◽  
pp. e01605-17
Author(s):  
Alexey Fomenkov ◽  
Tamas Vincze ◽  
Fana Mersha ◽  
Richard J. Roberts

ABSTRACT Bacillus caldolyticus NEB414 is the original source strain for the restriction enzyme BclI. Its complete sequence and full methylome were determined using single-molecule real-time sequencing.


2016 ◽  
Vol 4 (6) ◽  
Author(s):  
Azadeh Saffarian ◽  
Céline Mulet ◽  
Régis Tournebize ◽  
Tomoaki Naito ◽  
Philippe J. Sansonetti ◽  
...  

We report here the complete genome sequence of Delftia tsuruhatensis CM13, isolated from murine proximal colonic tissue. The genome assembly using PacBio single-molecule real-time sequencing resulted in a single scaffold of 7.19 Mb.


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