scholarly journals Complete Genome Sequence of a Phenanthrene Degrader, Burkholderia sp. HB-1 (NBRC 110738)

2015 ◽  
Vol 3 (6) ◽  
Author(s):  
Yoshiyuki Ohtsubo ◽  
Azusa Moriya ◽  
Hiromi Kato ◽  
Natsumi Ogawa ◽  
Yuji Nagata ◽  
...  

The phenanthrene-degrading Burkholderia sp. HB-1 was isolated from a phenanthrene-enrichment culture seeded with a pristine farm soil sample. We report the complete genome sequence of HB-1, which has been deposited to the stock culture (NBRC 110738) at Biological Resource Center, National Institute of Technology and Evaluation (NITE), Tokyo, Japan. The genome of strain HB-1 comprises two circular chromosomes of 4.1 Mb and 3.1 Mb. The finishing was facilitated by the computational tools GenoFinisher, AceFileViewer, and ShortReadManager.

2015 ◽  
Vol 3 (6) ◽  
Author(s):  
Yoshiyuki Ohtsubo ◽  
Yuji Nagata ◽  
Mitsuru Numata ◽  
Kieko Tsuchikane ◽  
Akira Hosoyama ◽  
...  

Sphingopyxis macrogoltabida strain 203, the type strain of the species, grew on polyethylene glycol (PEG) and has been deposited to the stock culture at the Biological Resource Center, National Institute of Technology and Evaluation (NITE), under the number NBRC 15033. Here, we report the complete genome sequence of strain NBRC 15033. Unfortunately, genes for PEG degradation were missing.


2015 ◽  
Vol 3 (6) ◽  
Author(s):  
Yoshiyuki Ohtsubo ◽  
Yuji Nagata ◽  
Mitsuru Numata ◽  
Kieko Tsuchikane ◽  
Akira Hosoyama ◽  
...  

Microbacterium (formerly Corynebacterium ) sp. No. 7 was isolated from activated sludge as a polypropylene glycol (PPG)-assimilating bacterial strain. Its oxidative PPG degradation has been proposed on the basis of PPG dehydrogenase activity and the metabolic products. Here, we report the complete genome sequence of Microbacterium sp. No. 7. The genome of the strain No. 7 is composed of a 4,599,046-bp circular chromosome and two linear plasmids. The whole finishing was conducted in silico with aids of the computational tools GenoFinisher and AceFileViewer. Strain No. 7 is available from the Biological Resource Center, National Institute of Technology and Evaluation (NITE) (Tokyo, Japan).


2015 ◽  
Vol 3 (6) ◽  
Author(s):  
Yoshiyuki Ohtsubo ◽  
Yuji Nagata ◽  
Mitsuru Numata ◽  
Kieko Tsuchikane ◽  
Akira Hosoyama ◽  
...  

Strain EY-1 was isolated from a microbial consortium growing on a random polymer of ethylene oxide and propylene oxide. Strain EY-1 grew on polyethylene glycol and polypropylene glycol and identified as Sphingopyxis macrogoltabida . Here, we report the complete genome sequence of Sphingopyxis macrogoltabida EY-1. The genome of strain EY-1 is comprised of a 4.76-Mb circular chromosome, and five plasmids. The whole finishing was conducted in silico , with aids of computational tools GenoFinisher and AceFileViewer. Strain EY-1 is available from Biological Resource Center, National Institute of Technology and Evaluation (Tokyo, Japan) (NITE).


2015 ◽  
Vol 3 (5) ◽  
Author(s):  
Yoshiyuki Ohtsubo ◽  
Yuji Nagata ◽  
Mitsuru Numata ◽  
Kieko Tsuchikane ◽  
Akira Hosoyama ◽  
...  

Strain 113P3 was isolated from activated sludge and identified as a polyvinyl alcohol (PVA)-degrading Pseudomonas species; it was later reidentified as Sphingopyxis species. Only three genes are directly relevant to the metabolism of PVA and comprise the pva operon, which was deposited as accession no. AB190228. Here, we report the complete genome sequence of strain 113P3, which has been conserved as a stock culture (NBRC 111507) at the Biological Resource Center, National Institute of Technology and Evaluation (NITE) (Tokyo, Japan). The genome of strain 113P3 is composed of a 4.4-Mb circular chromosome and a 243-kb plasmid. The whole finishing was conducted in silico except for four PCRs. The sequence corresponding to AB190288 exists on the chromosome.


2020 ◽  
Vol 9 (30) ◽  
Author(s):  
Victoria N. Lydick ◽  
Douglas B. Rusch ◽  
Blake Ushijima ◽  
Julia C. van Kessel

ABSTRACT Here, we report the complete genome sequence of Vibrio coralliilyticus OCN008, a marine bacterium that infects reef-building coral. Previous sequencing efforts yielded an incomplete sequence (210 contigs). We used Nanopore and Illumina sequencing data to obtain complete sequences of the two circular chromosomes (3.48 and 1.91 Mb) and one megaplasmid (244.69 kb).


2017 ◽  
Vol 5 (23) ◽  
Author(s):  
Filip Zekic ◽  
Brian Weselowski ◽  
Ze-Chun Yuan

ABSTRACT Here, we report the complete genome sequence of the phosphate-solubilizing bacterium Burkholderia cenocepacia CR318, consisting of three circular chromosomes of 3,511,146 bp, 3,097,552 bp, and 1,056,069 bp. The data presented will facilitate further insight into the mechanisms of phosphate solubilization and its application for agricultural and ecological sustainability.


Author(s):  
Skyler Adams ◽  
Gabrielle Spotz ◽  
Riley Babcock ◽  
Chloe Butler ◽  
Samantha Conger ◽  
...  

Microbacteriophage Fizzles has a 62,078-bp linear double-stranded DNA genome sequence, predicted to contain 104 protein-coding genes. Fizzles is a Siphoviridae actinobacteriophage isolated from an ant hill soil sample collected in Stephenville, TX. Microbacteriophage Fizzles has >83.6% nucleotide identity with microbacteriophages Squash and Nike.


2017 ◽  
Vol 5 (34) ◽  
Author(s):  
Soumaya Najah ◽  
Teik Min Chong ◽  
Claude Gerbaud ◽  
Kok-Gan Chan ◽  
Lotfi Mellouli ◽  
...  

ABSTRACT Streptomyces sp. TN58, isolated from a Tunisian soil sample, produces several natural products, including acyl alpha-l-rhamnopyranosides. It possesses a 7.6-Mb linear chromosome. This is, to our knowledge, the first genome sequence of a microorganism known to produce acyl alpha-l-rhamnopyranosides, and it will be helpful to study the biosynthesis of these specialized metabolites.


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