Whole-Genome Sequence of
                    Leptospira interrogans
                    Serovar Hardjo Subtype Hardjoprajitno Strain Norma, Isolated from Cattle in a Leptospirosis Outbreak in Brazil

Leptospirosis is caused by pathogenic bacteria of the genus Leptospira spp. This neglected re-emergent disease has global distribution and relevance in veterinary production. Here, we report the whole-genome sequence and annotation of Leptospira interrogans serovar Hardjo subtype Hardjoprajitno strain Norma, isolated from cattle in a livestock leptospirosis outbreak in Brazil.

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First Genome Sequence ofLeptospira interrogansSerovar Pomona, Isolated from a Bovine Abortion

Genome Announcements ◽

10.1128/genomea.00345-16 ◽

2016 ◽

Vol 4 (3) ◽

Cited By ~ 4

Author(s):

Vanina Varni ◽

Ariel Koval ◽

Ariel Nagel ◽

Paula Ruybal ◽

Karina Caimi ◽

...

Keyword(s):

Genome Sequence ◽

Global Distribution ◽

Whole Genome Sequence ◽

Leptospira Interrogans ◽

Whole Genome ◽

Bovine Abortion

Leptospirosis is a widespread zoonosis and a re-emergent disease of global distribution with major relevance in veterinary production. Here, we report the whole-genome sequence ofLeptospira interrogansserovar Pomona strain AKRFB, isolated from a bovine abortion during a leptospirosis outbreak in Argentina.

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Whole-Genome Sequence of Pseudomonas fluorescens EK007-RG4, a Promising Biocontrol Agent against a Broad Range of Bacteria, Including the Fire Blight Bacterium Erwinia amylovora

Genome Announcements ◽

10.1128/genomea.00026-17 ◽

2017 ◽

Vol 5 (13) ◽

Author(s):

Roghayeh Habibi ◽

Saeed Tarighi ◽

Javad Behravan ◽

Parissa Taheri ◽

Annelise Helene Kjøller ◽

...

Keyword(s):

Pseudomonas Fluorescens ◽

Genome Sequence ◽

Pathogenic Bacteria ◽

Fire Blight ◽

Erwinia Amylovora ◽

Whole Genome Sequence ◽

Whole Genome ◽

Content Type ◽

Pear Tree ◽

Blight Disease

ABSTRACT Here, we report the first draft whole-genome sequence of Pseudomonas fluorescens strain EK007-RG4, which was isolated from the phylloplane of a pear tree. P. fluorescens EK007-RG4 displays strong antagonism against Erwinia amylovora, the causal agent for fire blight disease, in addition to several other pathogenic and non-pathogenic bacteria.

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A potential genomic recombination site upstream of the rfb locus in Leptospira interrogans is associated with serogroup Serjoe and serovar Hardjo classification

10.1101/771931 ◽

2019 ◽

Author(s):

Maria Raquel V. Cosate ◽

Tetsu Sakamoto ◽

Élvio C. Moreira ◽

José Miguel Ortega ◽

Rômulo C. Leite ◽

...

Keyword(s):

Comparative Genomics ◽

Genome Sequence ◽

Livestock Production ◽

Whole Genome Sequence ◽

Leptospira Interrogans ◽

Whole Genome ◽

Bacterial Strains ◽

Recombination Site ◽

Reproductive Disease ◽

Genomic Recombination

AbstractLeptospirosis is a zoonotic disease caused by pathogenic spirochetes of the genus Leptospira. It has a global distribution and affects domestic animals, including cattle. In livestock production, Leptospira interrogans serogroup Sejroe serovar Hardjo is the major reproductive disease leading to economic losses. The whole-genome sequence of the first Brazilian clinical isolate classified as L. interrogans serogroup Sejroe serovar Hardjo strain Norma enabled the evaluation of its genomic features. Here, we investigated particularities of this isolate, obtained from a leptospirosis outbreak. Bioinformatic analysis using the L. interrogans serovar Hardjo str. Norma was applied as a reference for genomic evaluation and comparative analysis among L. interrogans and L. borgpetersenii serovars. Our data suggest the occurrence of genomic recombination in L. interrogans serovar Hardjo str. Norma encompassing 45 Kb located upstream of the rfb locus. A hallmark of genetic evolution was predicted through an orthologue analysis that identified that sugar enzymes associated with carbohydrate and lipid biosynthesis and metabolism composed this genetic module. Comparative genomics revealed a wide range of relatedness among the bacterial strains of serogroup Sejroe that are classified as L. interrogans and L. borgpetersenii species. Furthermore, identification of an IS3 family suggests a genetic recombination site in L. interrogans serovar Hardjo str. Norma that is distinct among L. interrogans serovars and may contribute to clarify the taxonomic classification of Leptospira spp.Impact StatementLeptospirosis remains an important neglected disease with worldwide distribution. This zoonotic disease impacts in the livestock production and the bovine infection is currently associated to species L. borgpetersenii and L. interrogans serovar Hardjo. L.interrogans serovar Hardjo infection is recognized as reproductive disease associated with abortion and economic lost. In this context, we studied a unique whole genome sequence of L. interrogans serovar Hardjo subtype Hardjo-prajitno isolated from bovine leptospirosis outbreaks in Brazilian dairy farm, one of the greatest country of milk production in world. We compared L. interrogans and L. borgpetersenii genomes with L. interrogans serogroup Sejroe serovar Hardjo subtype Hardjo-prajitno focusing on rfb locus and sugars biosynthesis. Leptospira spp. taxonomy and serology information are strictly associated with rfb locus and we found high correlation among bacterial strains classified in serogroup Sejroe. Although L. interrogans and L. borgpetersenii classified in serogroup Sejroe possess a greater genetic correlation, we uniquely identified that serovar Hardjo strains often possess identical loci carrying predicted sugar biossinthesis genes and mobile elements. The Sru (Sejroe specific Rfb Upstream locus) locus associated to rfb locus probably contribute to Leptospira spp. genetic information concerning serogroup and serovar degrees of taxonomic and serology in this microbiology field.Data SummaryAll Leptospira spp. genome sequences used in this study were retrieved from National Center for Biotechnology Information (NCBI) (Table 1) with NCBI ID: NZ_CP006723.1, NZ_CP012603.1, NC_004342.2, NZ_CP011934.1, NZ_AKXA02000040.1, NZ_CP013147.1, NZ_CP012029.1, NZ_CP015048.1, NC_008508.1, GCA_000216175.3,GCA_000244115.3, NC005823.1, GCA_000244395.3, GCA_000346975.1.HighlightsThe study identified potential molecular features associated with serovar Hardjo subtype Hardjo-prajitno, present in both L. interrogans and L. borgpetersenii, by comparative genomics.A new potential recombinant site found upstream of the rfb locus contains proteins that correlate with serogroup taxonomy in the Leptospira genus.The proteins encoded in the recombinant locus are associated with the synthesis of serological surface determinants such as carbohydrates and lipopolysaccharides.

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The ADP-Ribosylating Toxins of Salmonella

Toxins ◽

10.3390/toxins11070416 ◽

2019 ◽

Vol 11 (7) ◽

pp. 416 ◽

Cited By ~ 4

Author(s):

Cheng ◽

Wiedmann

Keyword(s):

Genome Sequence ◽

Pathogenic Bacteria ◽

Foodborne Pathogen ◽

Structure And Function ◽

Current Understanding ◽

Whole Genome Sequence ◽

Whole Genome ◽

Susceptible Host ◽

And Function ◽

Typhoid Toxin

A number of pathogenic bacteria utilize toxins to mediate disease in a susceptible host. The foodborne pathogen Salmonella is one of the most important and well-studied bacterial pathogens. Recently, whole genome sequence characterizations revealed the presence of multiple novel ADP-ribosylating toxins encoded by a variety of Salmonella serovars. In this review, we discuss both the classical (SpvB) and novel (typhoid toxin, ArtAB, and SboC/SeoC) ADP-ribosylating toxins of Salmonella, including the structure and function of these toxins and our current understanding of their contributions to virulence.

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Draft Genome Sequence of Aspergillus persii NIBRFGC000004109, Which Has Antibacterial Activity against Plant-Pathogenic Bacteria

Genome Announcements ◽

10.1128/genomea.00932-17 ◽

2017 ◽

Vol 5 (38) ◽

Author(s):

Jung A. Kim ◽

Jongbum Jeon ◽

Sook-Young Park ◽

Ki-Tae Kim ◽

Gobong Choi ◽

...

Keyword(s):

Antibacterial Activity ◽

Genome Sequence ◽

Pathogenic Bacteria ◽

Draft Genome ◽

Whole Genome Sequence ◽

Whole Genome ◽

Penicillic Acid ◽

Content Type ◽

Plant Pathogenic Bacteria ◽

Xanthomonas Arboricola

ABSTRACT The fungus Aspergillus persii strain NIBRFGC000004109 is capable of producing penicillic acid and showed antibacterial activity against various plant-pathogenic bacteria, including Xanthomonas arboricola pv. pruni. Here, we report the first draft whole-genome sequence of A. persii. The assembly comprises 38,414,373 bp, with 12 scaffolds.

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Putative novel cps loci in a large global collection of pneumococci

Microbial Genomics ◽

10.1099/mgen.0.000274 ◽

2019 ◽

Vol 5 (7) ◽

Cited By ~ 3

Author(s):

Andries J. van Tonder ◽

Rebecca A. Gladstone ◽

Stephanie W. Lo ◽

Moon H. Nahm ◽

Mignon du Plessis ◽

...

Keyword(s):

Geographical Distribution ◽

Genome Sequence ◽

Sequence Data ◽

Global Distribution ◽

Whole Genome Sequence ◽

Whole Genome ◽

Genome Sequence Data ◽

Immunological Analysis ◽

Polysaccharide Capsule

The pneumococcus produces a polysaccharide capsule, encoded by the cps locus, that provides protection against phagocytosis and determines serotype. Nearly 100 serotypes have been identified with new serotypes still being discovered, especially in previously understudied regions. Here we present an analysis of the cps loci of more than 18 000 genomes from the Global Pneumococcal Sequencing (GPS) project with the aim of identifying novel cps loci with the potential to produce previously unrecognized capsule structures. Serotypes were assigned using whole genome sequence data and 66 of the approximately 100 known serotypes were included in the final dataset. Closer examination of each serotype’s sequences identified nine putative novel cps loci (9X, 11X, 16X, 18X1, 18X2, 18X3, 29X, 33X and 36X) found in ~2.6 % of the genomes. The large number and global distribution of GPS genomes provided an unprecedented opportunity to identify novel cps loci and consider their phylogenetic and geographical distribution. Nine putative novel cps loci were identified and examples of each will undergo subsequent structural and immunological analysis.

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