scholarly journals Whole-Genome Sequence of a Beijing Extensively Drug-Resistant Mycobacterium tuberculosis Clinical Isolate from Buenaventura, Colombia

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
N. Alvarez ◽  
D. Haft ◽  
U. A. Hurtado ◽  
J. Robledo ◽  
F. Rouzaud
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Clinical Isolate ◽  
Genome Sequence ◽  
Multidrug Resistant ◽  
Whole Genome Sequence ◽  
Whole Genome ◽  
Drug Resistant ◽  
Extensively Drug Resistant

Extensively drug-resistant Mycobacterium tuberculosis (XDR-TB) has been reported to the WHO by 100 countries, including Colombia. An estimated 9.0% of people with multidrug-resistant TB have XDR-TB. We report the genome sequence of a Beijing XDR-TB clinical isolate from Buenaventura, Colombia. The genome sequence is composed of 4,298,162 bp with 4,359 genes.

Whole genome sequence analysis of multidrug-resistant Mycobacterium tuberculosis Beijing isolates from an outbreak in Thailand

2015 ◽  
Vol 290 (5) ◽  
pp. 1933-1941 ◽  
Author(s):  
Sanjib Mani Regmi ◽  
Angkana Chaiprasert ◽  
Supasak Kulawonganunchai ◽  
Sissades Tongsima ◽  
Olabisi Oluwabukola Coker ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Sequence Analysis ◽  
Genome Sequence ◽  
Multidrug Resistant ◽  
Whole Genome Sequence ◽  
Whole Genome ◽  
Genome Sequence Analysis

scholarly journals Whole genome analysis of extensively drug resistant Mycobacterium tuberculosis strains in Peru

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
David Santos-Lazaro ◽  
Ronnie G. Gavilan ◽  
Lely Solari ◽  
Aiko N. Vigo ◽  
Zully M. Puyen
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Multidrug Resistant ◽  
Annual Number ◽  
Whole Genome ◽  
Drug Resistant ◽  
Resistance Mutations ◽  
Whole Genome Analysis ◽  
Evolutionary Patterns ◽  
Resistant Tuberculosis ◽  
Extensively Drug Resistant

AbstractPeru has the highest burden of multidrug-resistant tuberculosis in the Americas region. Since 1999, the annual number of extensively drug-resistant tuberculosis (XDR-TB) Peruvian cases has been increasing, becoming a public health challenge. The objective of this study was to perform genomic characterization of Mycobacterium tuberculosis strains obtained from Peruvian patients with XDR-TB diagnosed from 2011 to 2015 in Peru. Whole genome sequencing (WGS) was performed on 68 XDR-TB strains from different regions of Peru. 58 (85.3%) strains came from the most populated districts of Lima and Callao. Concerning the lineages, 62 (91.2%) strains belonged to the Euro-American Lineage, while the remaining 6 (8.8%) strains belonged to the East-Asian Lineage. Most strains (90%) had high-confidence resistance mutations according to pre-established WHO-confident grading system. Discordant results between microbiological and molecular methodologies were caused by mutations outside the hotspot regions analysed by commercial molecular assays (rpoB I491F and inhA S94A). Cluster analysis using a cut-off ≤ 10 SNPs revealed that only 23 (34%) strains evidenced recent transmission links. This study highlights the relevance and utility of WGS as a high-resolution approach to predict drug resistance, analyse transmission of strains between groups, and determine evolutionary patterns of circulating XDR-TB strains in the country.

scholarly journals Whole-genome sequence analysis and comparisons between drug-resistance mutations and minimum inhibitory concentrations of Mycobacterium tuberculosis isolates causing M/XDR-TB

PLoS ONE ◽  
2020 ◽  
Vol 15 (12) ◽  
pp. e0244829
Author(s):  
Ditthawat Nonghanphithak ◽  
Orawee Kaewprasert ◽  
Pratchakan Chaiyachat ◽  
Wipa Reechaipichitkul ◽  
Angkana Chaiprasert ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Mycobacterium Tuberculosis ◽  
Drug Susceptibility ◽  
Multidrug Resistant ◽  
Whole Genome Sequence ◽  
Whole Genome ◽  
Drug Resistant ◽  
Resistance Mutations ◽  
Tb 34 ◽  
Susceptibility Tests

Drug resistance (DR) remains a major challenge for tuberculosis (TB) control. Whole-genome sequencing (WGS) provides the highest genetic resolution for genotypic drug-susceptibility tests (DST). We compared DST profiles of 60 Mycobacterium tuberculosis isolates which were drug resistant according to agar proportion tests (one poly DR-TB, 34 multidrug-resistant TB and 25 extensively drug-resistant TB). We additionally performed minimum inhibitory concentration (MIC) tests using Sensititre MYCOTBI plates (MYCOTB) and a WGS-based DST. Agreement between WGS-based DST and MYCOTB was high for all drugs except ethambutol (65%) and ethionamide (62%). Isolates harboring the -15 c/t inhA promoter mutation had a significantly lower MIC for isoniazid than did isolates with the katG Ser315Thr mutation (p < 0.001). Similar patterns were seen for ethambutol (embB Gly406Asp vs. embB Met306Ile), streptomycin (gid Gly73Ala vs. rpsL Lys43Arg), moxifloxacin (gyrA Ala90Val vs. gyrA Asp94Gly) and rifabutin (rpoB Asp435Phe/Tyr/Val vs. rpoB Ser450Leu). For genotypic heteroresistance, isolates with lower proportion of mapped read tended to has lower MIC of anti-TB drugs than those with higher proportion. These results emphasize the high applicability of WGS for determination of DR-TB and the association of particular mutations with MIC levels.

scholarly journals Whole-Genome Sequence of a Multidrug-Resistant Clinical Isolate of Acinetobacter lwoffii

2011 ◽  
Vol 193 (19) ◽  
pp. 5549-5550 ◽  
Author(s):  
Y. Hu ◽  
W. Zhang ◽  
H. Liang ◽  
L. Liu ◽  
G. Peng ◽  
...  
Keyword(s):  
Clinical Isolate ◽  
Genome Sequence ◽  
Multidrug Resistant ◽  
Whole Genome Sequence ◽  
Whole Genome

scholarly journals Draft Genome Sequence of an Extensively Drug-Resistant Mycobacterium tuberculosis Clinical Isolate, 3485_MTB, from Nur-Sultan, Kazakhstan

2020 ◽  
Vol 9 (10) ◽  
Author(s):  
Pavel Tarlykov ◽  
Sabina Atavliyeva ◽  
Arike Alenova ◽  
Yerlan Ramankulov
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Clinical Isolate ◽  
Genome Sequence ◽  
Draft Genome ◽  
Genetic Variations ◽  
Draft Genome Sequence ◽  
Drug Resistant ◽  
Content Type ◽  
Extensively Drug Resistant

Here, we report the draft genome sequence of an extensively drug-resistant Mycobacterium tuberculosis clinical isolate, 3485_MTB, from Nur-Sultan, Kazakhstan. The genome sequence is composed of 4,836,003 bp. The genome will provide more data on the genetic variations occurring in local drug-resistant isolates.

scholarly journals First Whole-Genome Sequence of a Clinical Isolate of Multidrug-Resistant Mycobacterium bovis BCG

2014 ◽  
Vol 2 (4) ◽  
Author(s):  
A. Renvoise ◽  
S. Pang ◽  
C. Bernard ◽  
F. Brossier ◽  
N. Veziris ◽  
...  
Keyword(s):  
Clinical Isolate ◽  
Mycobacterium Bovis ◽  
Genome Sequence ◽  
Multidrug Resistant ◽  
Whole Genome Sequence ◽  
Whole Genome ◽  
Mycobacterium Bovis Bcg

scholarly journals Whole-Genome Sequence of Drug-Resistant Mycobacterium tuberculosis Strain S7, Isolated from a Patient with Pulmonary Tuberculosis

2020 ◽  
Vol 9 (17) ◽  
Author(s):  
Aparna Meher ◽  
Hritusree Guha ◽  
Raghuveer Varma Pemmadi ◽  
Subia Akram ◽  
Hossain M. Faruquee ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Genome Sequence ◽  
Whole Genome Sequence ◽  
Whole Genome ◽  
Drug Resistant ◽  
Content Type ◽  
Pulmonary Tb ◽  
Significant Challenge ◽  
The Past ◽  
Mycobacterium Tuberculosis Strain

Over the past decades, drug-resistant Mycobacterium tuberculosis strains have presented a significant challenge, with inadequate diagnosis of tuberculosis (TB) cases. Here, we report the draft whole-genome sequence of drug-resistant M. tuberculosis strain S7, which was isolated from a patient from Tripura, India, who was diagnosed with pulmonary TB.

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