scholarly journals Complete Genome Sequence of Pseudomonas brassicacearum LBUM300, a Disease-Suppressive Bacterium with Antagonistic Activity toward Fungal, Oomycete, and Bacterial Plant Pathogens

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Amy Novinscak ◽  
Vijay J. Gadkar ◽  
David L. Joly ◽  
Martin Filion

Pseudomonas brassicacearum LBUM300, a plant rhizosphere-inhabiting bacterium, produces 2,4-diacetylphloroglucinol and hydrogen cyanide and has shown antagonistic activity against the plant pathogens Verticillium dahliae , Phytophthora cactorum , and Clavibacter michiganensis subsp. michiganensis . Here, we report the complete genome sequence of P. brassicacearum LBUM300.

2019 ◽  
Vol 11 (12) ◽  
pp. 3529-3533
Author(s):  
Pavelas Sazinas ◽  
Morten Lindqvist Hansen ◽  
May Iren Aune ◽  
Marie Højmark Fischer ◽  
Lars Jelsbak

Abstract Many of the soil-dwelling Pseudomonas species are known to produce secondary metabolite compounds, which can have antagonistic activity against other microorganisms, including important plant pathogens. It is thus of importance to isolate new strains of Pseudomonas and discover novel or rare gene clusters encoding bioactive products. In an effort to accomplish this, we have isolated a bioactive Pseudomonas strain DTU12.1 from leaf-covered soil in Denmark. Following genome sequencing with Illumina and Oxford Nanopore technologies, we generated a complete genome sequence with the length of 5,943,629 base pairs. The DTU12.1 strain contained a complete gene cluster for a rare thioquinolobactin siderophore, which was previously described as possessing bioactivity against oomycetes and several fungal species. We placed the DTU12.1 strain within Pseudomonas gessardii subgroup of fluorescent pseudomonads, where it formed a distinct clade with other Pseudomonas strains, most of which also contained a complete thioquinolobactin gene cluster. Only two other Pseudomonas strains were found to contain the gene cluster, though they were present in a different phylogenetic clade and were missing a transcriptional regulator of the whole cluster. We show that having the complete genome sequence and establishing phylogenetic relationships with other strains can enable us to start evaluating the distribution and evolutionary origins of secondary metabolite clusters.


2018 ◽  
Vol 6 (10) ◽  
Author(s):  
Cecilia Miccoli ◽  
Davide Palmieri ◽  
Filippo De Curtis ◽  
Giuseppe Lima ◽  
Giuseppe Ianiri ◽  
...  

ABSTRACTRhodotorula kratochvilovaestrain LS11 is a biocontrol agent (BCA) selected for its antagonistic activity against several plant pathogens both in the field and postharvest. Genome assembly includes 62 contigs for a total of 22.56 Mbp and a G+C content of 66.6%. Genome annotation predicts 7,642 protein-encoding genes.


2015 ◽  
Vol 214 ◽  
pp. 103-104 ◽  
Author(s):  
Chungyun Bae ◽  
Eom-Ji Oh ◽  
Han-Beoyl Lee ◽  
Byung-Yong Kim ◽  
Chang-Sik Oh

2015 ◽  
Vol 211 ◽  
pp. 97-98 ◽  
Author(s):  
Hyun-Hee Lee ◽  
Jungwook Park ◽  
Jae Yun Lim ◽  
Hun Kim ◽  
Gyung Ja Choi ◽  
...  

2017 ◽  
Vol 5 (48) ◽  
Author(s):  
Pingping Sun ◽  
Jianchao Cui ◽  
Xiaohui Jia ◽  
Wenhui Wang

ABSTRACT Bacillus velezensis L-1 is an effective biocontrol agent against pear diseases. Here, we report the complete genome sequence of B. velezensis L-1 in which clusters related to the biosynthesis of secondary metabolites were predicted. This genome provides insights into the possible biocontrol mechanisms and furthers application of this specific bacterium.


2020 ◽  
Vol 9 (26) ◽  
Author(s):  
Shivani Khatri ◽  
Pavelas Sazinas ◽  
Yashbir S. Shivay ◽  
Shilpi Sharma ◽  
Lars Jelsbak

ABSTRACT Here, we report the annotated whole-genome sequence of Pseudomonas sp. strain SK, isolated in India from organic wheat rhizosphere. This strain has proved to be a species with potential biocontrol activity against soilborne plant pathogens based on antiSMASH analysis.


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