scholarly journals Effect of Growth Rate and Glucose Concentration on the Activity of the Phosphoenolpyruvate Phosphotransferase System in Streptococcus mutans Ingbritt Grown in Continuous Culture

1979 ◽  
Vol 23 (2) ◽  
pp. 224-231 ◽  
Author(s):  
D. C. Ellwood ◽  
P. J. Phipps ◽  
I. R. Hamilton
Keyword(s):  
Growth Rate ◽  
Continuous Culture ◽  
Streptococcus Mutans ◽  
Glucose Concentration ◽  
Phosphotransferase System

scholarly journals Glucose transport of Escherichia coli growing in glucose-limited continuous culture

1979 ◽  
Vol 178 (1) ◽  
pp. 97-101 ◽  
Author(s):  
I S Hunter ◽  
H L Kornberg
Keyword(s):  
Escherichia Coli ◽  
Continuous Culture ◽  
Glucose Concentration ◽  
Wild Type ◽  
Phosphotransferase System ◽  
Glucose Limitation ◽  
Low Glucose ◽  
Rate Limiting ◽  
Excess Glucose ◽  
Stimulation Of

Dilute cultures of wild-type Escherichia coli K12 and of derivatives impaired in one or other Enzyme-II component of the glucose phosphotransferase system were grown in continuous culture under glucose limitation. Cells harvested from the chemostat took up [U-14C]glucose from 0.1 mM solutions at rates directly related to the rates at which those cells had grown; the activity of the phosphotransferase system in those cells, rendered permeable with optimal accounts of toluene, parallels the ability of the cells to take up glucose. The capacity of these systems was rate-limiting for growth under the negligibly low glucose concentration in the chemostat, but was adequate to account for the stimulation of respiration observed when the cells were presented suddenly with excess glucose.

CONTINUOUS CULTURE OF BRUCELLA ABORTUS S.19

1961 ◽  
Vol 7 (4) ◽  
pp. 491-505 ◽  
Author(s):  
Andreas H. W. Hauschild ◽  
Hilliard Pivnick
Keyword(s):  
Growth Rate ◽  
Continuous Culture ◽  
Dilution Rate ◽  
Brucella Abortus ◽  
Specific Growth ◽  
Limiting Factor ◽  
Continuous Growth ◽  
Viable Cells ◽  
Metabolic Products ◽  
Growth Of Bacteria

An apparatus is described for the continuous growth of bacteria. Brucella abortus S.19 has been grown in continuous culture for periods up to 3 weeks with populations up to 2 × 1011viable cells per ml and without the establishment of nonsmooth variants.Concentrations between 3 × 109and 2 × 1011cells per ml could be maintained as a function of the dilution rate without the requirement of a known limiting factor in the medium. In a series of steady-state conditions, the specific growth rate increased steadily up to 0.28 hour−1with decreasing population levels.Incidence of mutants was governed by the dilution rate and could also be reduced by various chelating substances.In continuous growth combined with continuous dialysis, population levels were approximately twice those obtained in continuous growth without dialysis. The effect of dialysis appears to be the continuous removal of growth-limiting metabolic products.

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