scholarly journals Isotypic analysis of Plasmodium falciparum-specific antibodies and their relation to protection in Madagascar.

1993 ◽  
Vol 61 (10) ◽  
pp. 4498-4500 ◽  
Author(s):  
B Dubois ◽  
P Deloron ◽  
P Astagneau ◽  
C Chougnet ◽  
J P Lepers
2005 ◽  
Vol 73 (9) ◽  
pp. 5903-5907 ◽  
Author(s):  
Salenna R. Elliott ◽  
Amy K. Brennan ◽  
James G. Beeson ◽  
Eyob Tadesse ◽  
Malcolm E. Molyneux ◽  
...  

ABSTRACT Antibodies targeting variant antigens on the surfaces of chondroitin sulfate A (CSA)-binding malaria-infected erythrocytes have been linked to protection against the complications of malaria in pregnancy. We examined the isotype/subtype profiles of antibodies that bound to variant surface antigens expressed by CSA-adherent Plasmodium falciparum in pregnant Malawian women with and without histologically defined placental malaria. Women in their first pregnancy with placental malaria produced significantly greater amounts of immunoglobulin G1 (IgG1) and IgG3 reactive with surface antigens of malaria-infected erythrocytes than uninfected women of the same gravidity. IgG1 and IgG3 levels in infected and control women in later pregnancies were similar to those in infected women in their first pregnancy. Levels of IgG2 and IgG4 were similarly low in infected and uninfected women of all gravidities. IgM that bound to the surface of CSA-adherent P. falciparum occurred in all groups of women and malaria-naïve controls. There was a significant correlation between IgG1 and IgG3 levels, indicating that women usually produced both subtypes. Levels of IgG1 and IgG3 correlated with the ability of serum or plasma to inhibit parasite adhesion to CSA. Taken together, these data suggest that IgG1 and IgG3 dominate the IgG response to placental-type variant surface antigens. They may function by blocking parasite adhesion to placental CSA, but given their cytophilic nature, they might also opsonize malaria-infected erythrocytes for interaction with Fc receptors on phagocytic cells.


2019 ◽  
Author(s):  
Duah Dwomoh ◽  
Bright Adu ◽  
Daniel Dodoo ◽  
Michael Theisen ◽  
Samuel Iddi ◽  
...  

Abstract Background Malaria antigen-specific antibodies and polymorphisms in host receptors involved in antibody functionality have been associated with different outcomes of Plasmodium falciparum infections. Thus, to identify key prospective malaria antigens for vaccine development, there is the need to evaluate the associations between malaria antibodies and antibody dependent host factors with more rigorous statistical methods. In this study, we employ suitable statistical models to evaluate the predictive performance of malaria-specific antibodies and host gene polymorphisms on P. falciparum infection in a longitudinal cohort study involving Ghanaian children.Methods Models with different functional forms were built using known predictors (age, sickle cell status, blood group status, parasite density, and mosquito bed net use) and malaria antigen-specific antibodies and FCGR3B polymorphisms shown to mediate antibody-dependent neutrophil function. The models were evaluated through visualization and assessment of differences between the Area Under the Receiver Operating Characteristic Curve and Brier Score estimated by suitable internal cross-validation designs.Results This study has found that the FCGR3B-c.233C>A genotype and IgG against AMA1 were relatively better compared to the other antibodies and FCGR3B genotypes in classifying or predicting malaria risk among children.Conclusions Apical Membrane Antigen 1 could be a key potential vaccine antigen while FCGR3B-c.233C>A under the additive and dominant models of inheritance could be an important modifier of the effect of malaria protective antibodies.


Sign in / Sign up

Export Citation Format

Share Document