scholarly journals Heterogeneous Expression and Release of CD14 by Human Gingival Fibroblasts: Characterization and CD14-Mediated Interleukin-8 Secretion in Response to Lipopolysaccharide

1998 ◽  
Vol 66 (7) ◽  
pp. 3043-3049 ◽  
Author(s):  
Shunji Sugawara ◽  
Akiko Sugiyama ◽  
Eiji Nemoto ◽  
Hidemi Rikiishi ◽  
Haruhiko Takada
Keyword(s):  
Inflammatory Diseases ◽  
Flow Cytometric Analysis ◽  
Interleukin 8 ◽  
Human Gingival Fibroblasts ◽  
Lung Fibroblasts ◽  
Gingival Tissue ◽  
Gingival Fibroblasts ◽  
Flow Cytometric ◽  
Heterogeneous Expression ◽  
Phosphatidylinositol Phospholipase C

ABSTRACT To identify the role in periodontal inflammatory diseases of human gingival fibroblasts (HGF), the major constituents of gingival tissue, the expression of CD14, a possible lipopolysaccharide (LPS) receptor, and the release of soluble CD14 (sCD14) by HGF were examined. Among the HGF samples from the nine donors tested, more than 50% of the HGF from five donors expressed CD14 but less than 20% of HGF from the other four donors did so, as determined by flow cytometric analysis. The CD14 expression on the cell surface was correlated with the expression of CD14 mRNA. The HGF and skin and lung fibroblasts tested expressed no CD18, which indicates that fibroblasts do not possess other LPS receptors, such as CD11b/CD18 and CD11c/CD18. The CD14 expression by the HGF was decreased after subculturing and was highest at the confluent stage of culture. The treatment of high-CD14-expressing (CD14high) HGF with phosphatidylinositol-phospholipase C reduced CD14 expression; this result and the increase in a 55-kDa CD14 indicate that the membrane CD14 (mCD14) on the HGF may be a 55-kDa glycosylphosphatidylinositol-anchored protein. CD14high HGF spontaneously released 48- and 57-kDa sCD14. The total release of sCD14 by the HGF was augmented by gamma interferon and Escherichia coli LPS in accordance with the increased expression of mCD14. The CD14high HGF secreted interleukin-8 in response to LPS, and the secretion was completely inhibited by anti-CD14 antibody. These results suggest that (i) HGF consist of populations that are heterogeneous on the basis of different levels of expression of CD14 and (ii) CD14high HGF secrete inflammatory cytokines in response to LPS via CD14.

Effects of alendronate and pamidronate on apoptosis and cell proliferation in cultured primary human gingival fibroblasts

2015 ◽  
Vol 34 (11) ◽  
pp. 1073-1082 ◽  
Author(s):  
SS Soydan ◽  
K Araz ◽  
FV Senel ◽  
E Yurtcu ◽  
F Helvacioglu ◽  
...  
Keyword(s):  
Oral Mucosa ◽  
Mtt Assay ◽  
In Vitro Study ◽  
Osteonecrosis Of The Jaw ◽  
Human Gingival Fibroblasts ◽  
Gingival Tissue ◽  
Proliferation Index ◽  
Gingival Fibroblasts ◽  
Inhibition Of Proliferation

Data arising from the recent literature directed the researchers to study on the degree and extent of bisphosphonate toxicity on oral mucosa in further detail. The aim of this study is to determine the half maximal inhibitory concentration of pamidronate (PAM) and alendronate (ALN) on human gingival fibroblasts in vitro using 3-[4.5-thiazol-2-yl]-2.5-diphenyltetrazolium bromide (MTT) assay and to evaluate the effects of both agents on the proliferation and apoptotic indices. Cells used in the study were generated from human gingival specimens and divided into alendronate ( n = 240), PAM ( n = 240), and control groups ( n = 60). Based on the MTT assay results, 10−4, 10−5, 10−6, and 10−7 M concentrations of both drugs were administered and the effects were evaluated for 6, 12, 24, 48, or 72 h periods. An indirect immunofluorescence technique was used to evaluate apoptotic (anti-caspase 3) and proliferation (anti-Ki67) indices. Toxicity of both PAM and ALN was found to be the most potent at 10−4–10−5 M range. The apoptotic index of PAM group was found to be significantly higher than ALN group for all concentrations especially at 24 h incubation time ( p < 0.05). The decrease in the proliferation index was found similar in first 48 h for both drugs; however, after 72 h of incubation decrease in proliferation index in PAM group was found to be significantly higher ( p < 0.05). Micromolar concentrations of not only PAM but also ALN rapidly affect cells generated from human oral gingival tissue by inducing apoptosis together with inhibition of proliferation. Cytotoxic effects of both ALN and PAM on primary human gingival fibroblasts, which cause significant changes in apoptotic and proliferative indices as shown in this in vitro study, suggests that the defective epithelialization of oral mucosa is possibly a major factor on the onset of bisphosphonate-related osteonecrosis of the jaw cases.

scholarly journals Sampling of nasal secretions for flow-cytometric analysis in inflammatory diseases of the nose and paranasal sinuses

2013 ◽  
Vol 47 (1) ◽  
pp. 30-35
Author(s):  
Peric Aleksandar ◽  
Vojvodic Danilo ◽  
Milojevic Milanko ◽  
Mladenovic Nenad ◽  
Vujovic Aleksandar ◽  
...  
Keyword(s):  
Paranasal Sinuses ◽  
Inflammatory Diseases ◽  
Flow Cytometric Analysis ◽  
Flow Cytometric ◽  
Nasal Secretions ◽  
Nose And Paranasal Sinuses

scholarly journals Expression of deubiquitinases in human gingiva and cultured human gingival fibroblasts

2021 ◽  
Author(s):  
Yong-Wei Fu
Keyword(s):  
Protein Expression ◽  
Inflammatory Diseases ◽  
Immunofluorescence Assay ◽  
Human Gingival Fibroblasts ◽  
Gingival Fibroblasts ◽  
Deubiquitinating Enzymes ◽  
Western Blots ◽  
Periodontal Tissues ◽  
Gene And Protein Expression ◽  
Human Gingiva

AbstractAlthough deubiquitinating enzymes (DUBs) such as CYLD, A20 and OTULIN are expressed in multiple tissues and thought to be linked with inflammatory diseases, their expression in periodontal tissues remains to be determined. This research was designed to assess the expression of CYLD, A20 and OTULIN in human gingiva, and to evaluate the regulation of these DUBs in human gingival fibroblasts (HGFs) upon different stimuli. Immunohistochemistry assay was conducted to determine the expression of CYLD, A20 and OTULIN in human gingiva. Immunofluorescence assay was employed to observe the protein expression of CYLD, A20 and OTULIN in HGFs. RT-PCR and western blots were carried out to assess gene and protein expression changes of these DUBs in HGFs upon LPS or TNF-α. CYLD, A20 and OTULIN were found to be expressed in human gingiva and HGFs. Further, the expression of CYLD, A20 and OTULIN in HGFs exhibited distinct regulation by different stimuli. Our findings suggest that CYLD, A20 and OTULIN might play a role in the progression of periodontitis.

Production of Cytokines Belonging to the Interleukin-8 Family by Human Gingival Fibroblasts Stimulated with Interleukin-1β in Culture

1993 ◽  
Vol 58 (1) ◽  
pp. 14-24 ◽  
Author(s):  
Hiroshi Odake ◽  
Fumitomo Koizumi ◽  
Shinji Hatakeyama ◽  
Isao Furuta ◽  
Hideo Nakagawa
Keyword(s):  
Interleukin 8 ◽  
Human Gingival Fibroblasts ◽  
Interleukin 1Β ◽  
Gingival Fibroblasts

Effects of Ginseng on the Proliferation of Human Lung Fibroblasts

2006 ◽  
Vol 34 (01) ◽  
pp. 137-146 ◽  
Author(s):  
Hye Hyun Yoo ◽  
Takako Yokozawa ◽  
Akiko Satoh ◽  
Ki Sung Kang ◽  
Hyun Young Kim
Keyword(s):  
Cell Growth ◽  
Early Stage ◽  
Human Fibroblasts ◽  
Flow Cytometric Analysis ◽  
Lung Fibroblasts ◽  
Population Doubling ◽  
Methanolic Extracts ◽  
Flow Cytometric ◽  
Heat Treated ◽  
White Ginseng

In this study, we investigated the effects of methanolic extracts of white ginseng (Panax ginseng C.A. MEYER) and two kinds of heat-treated ginseng made by steaming fresh ginseng at 100°C for 3 hours (HTG-100) or 120°C for 3 hours (HTG-120) on the cell growth of human fibroblasts. All of the tested ginseng extracts stimulated cell growth, although the effect of HTG-120 was weaker than that of the other extracts. However, none of the ginseng extracts exhibited any effect on the growth of old cells with a population doubling level (PDL) of 48.7. Flow cytometric analysis showed that ginseng extracts raised the population of cells in G 0/ G 1 phase after treatment for 24 hours, but did not exert any effect after treatment for 48 hours. These results suggest that ginsengs exert their cell growth-promoting action mainly on younger cells at an early stage of the cell cycle, and that this effect is closely associated with an increase in the population of cells in the G 0/ G 1 phase.

scholarly journals Ecklonia cava Extract Exerts Anti-Inflammatory Effect in Human Gingival Fibroblasts and Chronic Periodontitis Animal Model by Suppression of Pro-Inflammatory Cytokines and Chemokines

Foods ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1656
Author(s):  
Jae-In Jung ◽  
Seonyoung Kim ◽  
Seung-Min Baek ◽  
Soo-Im Choi ◽  
Gun-Hee Kim ◽  
...  
Keyword(s):  
Bone Resorption ◽  
Chronic Periodontitis ◽  
Nuclear Factor Kappa B ◽  
Human Gingival Fibroblasts ◽  
Ecklonia Cava ◽  
Gingival Tissue ◽  
Nuclear Factor ◽  
Gingival Fibroblasts ◽  
Gene Expressions ◽  
Inflammatory Effect

Periodontitis is one of the most common chronic inflammatory diseases. The anti-inflammatory effect of the extract from brown algae Ecklonia cava was analyzed in lipopolysaccharide (LPS)-stimulated human gingival fibroblasts (HGF-1), the most abundant cells in gingival tissue. The gene expressions of cyclooxygenase-2 and interleukin-6 were decreased by 78 and 50%, respectively, at 100 μg/mL Ecklonia cava extract (ECE) treatment. The gene expressions of matrix metalloproteases (MMP-2 and MMP-8) and chemokines (macrophage inflammatory protein 1-alpha and stromal cell-derived factor 1) were also significantly down-regulated by ECE treatment (p < 0.05). The increased reactive oxygen species (ROS) production in HGF-1 cells by LPS stimulation was decreased by 30% at 100 μg/mL ECE treatment. The mitogen-activated protein kinase pathway and the nuclear factor-kappa B (NF-κB) signal activated by ROS were suppressed by ECE in a dose-dependent manner. ECE treatment (400 mg/kg, 8 weeks) significantly improved alveolar bone resorption in the ligature-induced chronic periodontitis rat model. ECE supplementation also lowered elevated mRNA expression of the receptor activator of nuclear factor-kappa B (RANKL)/osteoprotegerin (OPG) in the gingival tissue (p < 0.05). Therefore, ECE mitigated gingival tissue destruction and bone resorption associated with chronic periodontitis condition.

scholarly journals Expression of deubiquitinases in human gingiva and cultured human gingival fibroblasts

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Yong-Wei Fu ◽  
Hong-Zhi Xu
Keyword(s):  
Protein Expression ◽  
Human Gingival Fibroblasts ◽  
Gingival Tissue ◽  
Gingival Fibroblasts ◽  
Western Blots ◽  
Tissue Samples ◽  
Periodontal Tissues ◽  
Tnf Α ◽  
Gene And Protein Expression ◽  
Human Gingiva

Abstract Background Although deubiquitinating enzymes (DUBs) such as CYLD, A20 and OTULIN are expressed in multiple tissues and thought to be linked with inflammatory diseases, their expression in periodontal tissues remains to be determined. This research was designed to assess the expression of CYLD, A20 and OTULIN in human gingiva, and to evaluate the regulation of these DUBs in human gingival fibroblasts (HGFs) upon different stimuli. Methods Immunohistochemistry assay was conducted to determine the expression of CYLD, A20 and OTULIN in human gingiva. Immunofluorescence assay was employed to observe the protein expression of CYLD, A20 and OTULIN in HGFs. RT-PCR and western blots were carried out to assess gene and protein expression changes of these DUBs in HGFs upon LPS or TNF-α. Results CYLD, A20 and OTULIN were found to be expressed in human gingiva and HGFs. The expression of CYLD, A20 and OTULIN was lower in the inflamed gingival tissue samples compared with the healthy gingival tissue samples. Further, the expression of CYLD, A20 and OTULIN in HGFs exhibited distinct regulation by different stimuli. TNF-α treatment markedly increased NF-κB activation in HGFs Conclusions Our findings suggest that CYLD, A20 and OTULIN might play a role in the progression of periodontitis.

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