fbpABC Gene Cluster in Neisseria meningitidis Is Transcribed as an Operon

ABSTRACT The neisserial fbpABC locus has been proposed to constitute a single transcriptional unit. To confirm this operonic arrangement, transcription assays using reverse transcriptase PCR amplification were conducted with Neisseria meningitidis. The presence of fbpAB and fbpBC transcripts obtained by priming cDNA synthesis with anfbpC-sequence-specific oligonucleotide indicates thatfbpABC is organized as a single expression unit. The ratio of fbpA to fbpABC mRNA was approximately between 10- to 20-fold, as determined by real-time quantitative PCR.

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Analysis of maternal microchimerism in rhesus monkeys (Macaca mulatta) using real-time quantitative PCR amplification of MHC polymorphisms

Chimerism ◽

10.4161/chim.27778 ◽

2014 ◽

Vol 5 (1) ◽

pp. 6-15 ◽

Cited By ~ 9

Author(s):

Sonia Bakkour ◽

Chris AR Baker ◽

Alice F Tarantal ◽

Li Wen ◽

Michael P Busch ◽

...

Keyword(s):

Real Time ◽

Quantitative Pcr ◽

Macaca Mulatta ◽

Rhesus Monkeys ◽

Pcr Amplification ◽

Real Time Quantitative Pcr ◽

Mhc Polymorphisms

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Optimized Real-Time Quantitative PCR Measurement of Male Fetal DNA in Maternal Plasma

Clinical Chemistry ◽

10.1373/clinchem.2005.051235 ◽

2005 ◽

Vol 51 (9) ◽

pp. 1598-1604 ◽

Cited By ~ 123

Author(s):

Bernhard Zimmermann ◽

Ahmad El-Sheikhah ◽

Kypros Nicolaides ◽

Wolfgang Holzgreve ◽

Sinuhe Hahn

Keyword(s):

Real Time ◽

Quantitative Pcr ◽

Pcr Amplification ◽

First Trimester ◽

Single Copy ◽

Maternal Plasma ◽

Probit Regression ◽

Real Time Quantitative Pcr ◽

Fetal Dna ◽

First Trimester Of Pregnancy

Abstract Background: Circulating fetal DNA (cfDNA) in maternal plasma has been measured to investigate its possible relationship with pregnancy-related disorders, including fetal trisomy 21 and preeclampsia. The circulating concentrations of single-copy fetal genes, however, are close to the detection limits of PCR methods. Methods: We optimized a protocol for the real-time quantitative PCR amplification of the multicopy sequence DYS14 on the Y-chromosome. This was compared with an established real-time PCR assay for the single-copy SRY gene. Results: By probit regression analysis, the measurements of male DNA by the DYS14 assay had a 10-fold lower detection limit (0.4 genome equivalents) than did measurements of SRY. For plasma samples from women in the first trimester of pregnancy, imprecision (CV) was 2%–22% when amplifying DYS14 compared with 26%–140% for SRY. Conclusions: The low copy numbers of fetal DNA in plasma of women in the first trimester of pregnancy cannot be measured precisely when targeting single-copy sequences. Better results are obtained by amplifying a sequence that is present in multiple copies per male genome.

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Selection of stable reference genes for real-time quantitative PCR in honey bee pesticide toxicity studies

Journal of Apicultural Research ◽

10.1080/00218839.2021.1950972 ◽

2021 ◽

pp. 1-11

Author(s):

Sanghyeon Kim ◽

Susie Cho ◽

Si Hyeock Lee

Keyword(s):

Real Time ◽

Quantitative Pcr ◽

Honey Bee ◽

Reference Genes ◽

Pesticide Toxicity ◽

Real Time Quantitative Pcr ◽

Toxicity Studies ◽

Selection Of

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Development of One-Step, Real-Time, Quantitative Reverse Transcriptase PCR Assays for Absolute Quantitation of Human Coronaviruses OC43 and 229E

Journal of Clinical Microbiology ◽

10.1128/jcm.43.11.5452-5456.2005 ◽

2005 ◽

Vol 43 (11) ◽

pp. 5452-5456 ◽

Cited By ~ 53

Author(s):

L. Vijgen ◽

E. Keyaerts ◽

E. Moes ◽

P. Maes ◽

G. Duson ◽

...

Keyword(s):

Reverse Transcriptase ◽

Real Time ◽

Absolute Quantitation ◽

Reverse Transcriptase Pcr ◽

One Step ◽

Pcr Assays ◽

Human Coronaviruses

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A papaya-specific gene, papain, used as an endogenous reference gene in qualitative and real-time quantitative PCR detection of transgenic papayas

European Food Research and Technology ◽

10.1007/s00217-008-0935-6 ◽

2008 ◽

Vol 228 (2) ◽

pp. 301-309 ◽

Cited By ~ 15

Author(s):

Wentao Xu ◽

Weibin Bai ◽

Feng Guo ◽

Yunbo Luo ◽

Yanfang Yuan ◽

...

Keyword(s):

Real Time ◽

Quantitative Pcr ◽

Reference Gene ◽

Pcr Detection ◽

Specific Gene ◽

Real Time Quantitative Pcr ◽

Endogenous Reference Gene ◽

Endogenous Reference

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Development of a rapid detection and quantification method of Karenia mikimotoi by real-time quantitative PCR

Harmful Algae ◽

10.1016/j.hal.2012.03.004 ◽

2012 ◽

Vol 17 ◽

pp. 83-91 ◽

Cited By ~ 41

Author(s):

Jian Yuan ◽

Tiezhu Mi ◽

Yu Zhen ◽

Zhigang Yu

Keyword(s):

Real Time ◽

Quantitative Pcr ◽

Rapid Detection ◽

Karenia Mikimotoi ◽

Real Time Quantitative Pcr ◽

Quantification Method ◽

Detection And Quantification

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Use of real-time quantitative PCR to investigate root and gall colonisation by co-inoculated isolates of the nematophagous fungusPochonia chlamydosporia

Annals of Applied Biology ◽

10.1111/j.1744-7348.2009.00333.x ◽

2009 ◽

Vol 155 (1) ◽

pp. 143-152 ◽

Cited By ~ 20

Author(s):

S.D. Atkins ◽

B. Peteira ◽

I.M. Clark ◽

B.R. Kerry ◽

P.R. Hirsch

Keyword(s):

Real Time ◽

Quantitative Pcr ◽

Real Time Quantitative Pcr

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Quantification and Stability of Human Adenoviruses and Polyomavirus JCPyV in Wastewater Matrices

Applied and Environmental Microbiology ◽

10.1128/aem.00965-06 ◽

2006 ◽

Vol 72 (12) ◽

pp. 7894-7896 ◽

Cited By ~ 193

Author(s):

Silvia Bofill-Mas ◽

Nestor Albinana-Gimenez ◽

Pilar Clemente-Casares ◽

Ayalkibet Hundesa ◽

Jesus Rodriguez-Manzano ◽

...

Keyword(s):

Real Time ◽

Quantitative Pcr ◽

High Stability ◽

Wastewater Sludge ◽

Human Polyomavirus ◽

Sewage Effluent ◽

Viral Contamination ◽

Human Adenoviruses ◽

Urban Sewage ◽

Real Time Quantitative Pcr

ABSTRACT Human adenoviruses (HAdV) and human polyomavirus JCPyV have been previously proposed as indicators of fecal viral contamination in the environment. Different wastewater matrices have been analyzed by applying real-time quantitative PCR procedures for the presence, quantity, and stability of a wide diversity of excreted HAdV and JCPyV. High quantities of HAdV and JCPyV were detected in sewage, effluent wastewater, sludge, and biosolid samples. Both viruses showed high stability in urban sewage. These results confirm the suitability of both viruses as indicators of human fecal viral pollution.

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