scholarly journals Analysis of maternal microchimerism in rhesus monkeys (Macaca mulatta) using real-time quantitative PCR amplification of MHC polymorphisms

Chimerism ◽  
2014 ◽  
Vol 5 (1) ◽  
pp. 6-15 ◽  
Author(s):  
Sonia Bakkour ◽  
Chris AR Baker ◽  
Alice F Tarantal ◽  
Li Wen ◽  
Michael P Busch ◽  
...  
Keyword(s):  
Real Time ◽  
Quantitative Pcr ◽  
Macaca Mulatta ◽  
Rhesus Monkeys ◽  
Pcr Amplification ◽  
Real Time Quantitative Pcr ◽  
Mhc Polymorphisms

scholarly journals fbpABC Gene Cluster in Neisseria meningitidis Is Transcribed as an Operon

2000 ◽  
Vol 68 (12) ◽  
pp. 7166-7171 ◽  
Author(s):  
Heng H. Khun ◽  
Vinay Deved ◽  
Howard Wong ◽  
B. Craig Lee
Keyword(s):  
Reverse Transcriptase ◽  
Neisseria Meningitidis ◽  
Real Time ◽  
Gene Cluster ◽  
Quantitative Pcr ◽  
Pcr Amplification ◽  
Transcriptional Unit ◽  
Cdna Synthesis ◽  
Reverse Transcriptase Pcr ◽  
Real Time Quantitative Pcr

ABSTRACT The neisserial fbpABC locus has been proposed to constitute a single transcriptional unit. To confirm this operonic arrangement, transcription assays using reverse transcriptase PCR amplification were conducted with Neisseria meningitidis. The presence of fbpAB and fbpBC transcripts obtained by priming cDNA synthesis with anfbpC-sequence-specific oligonucleotide indicates thatfbpABC is organized as a single expression unit. The ratio of fbpA to fbpABC mRNA was approximately between 10- to 20-fold, as determined by real-time quantitative PCR.

scholarly journals Optimized Real-Time Quantitative PCR Measurement of Male Fetal DNA in Maternal Plasma

2005 ◽  
Vol 51 (9) ◽  
pp. 1598-1604 ◽  
Author(s):  
Bernhard Zimmermann ◽  
Ahmad El-Sheikhah ◽  
Kypros Nicolaides ◽  
Wolfgang Holzgreve ◽  
Sinuhe Hahn
Keyword(s):  
Real Time ◽  
Quantitative Pcr ◽  
Pcr Amplification ◽  
First Trimester ◽  
Single Copy ◽  
Maternal Plasma ◽  
Probit Regression ◽  
Real Time Quantitative Pcr ◽  
Fetal Dna ◽  
First Trimester Of Pregnancy

Abstract Background: Circulating fetal DNA (cfDNA) in maternal plasma has been measured to investigate its possible relationship with pregnancy-related disorders, including fetal trisomy 21 and preeclampsia. The circulating concentrations of single-copy fetal genes, however, are close to the detection limits of PCR methods. Methods: We optimized a protocol for the real-time quantitative PCR amplification of the multicopy sequence DYS14 on the Y-chromosome. This was compared with an established real-time PCR assay for the single-copy SRY gene. Results: By probit regression analysis, the measurements of male DNA by the DYS14 assay had a 10-fold lower detection limit (0.4 genome equivalents) than did measurements of SRY. For plasma samples from women in the first trimester of pregnancy, imprecision (CV) was 2%–22% when amplifying DYS14 compared with 26%–140% for SRY. Conclusions: The low copy numbers of fetal DNA in plasma of women in the first trimester of pregnancy cannot be measured precisely when targeting single-copy sequences. Better results are obtained by amplifying a sequence that is present in multiple copies per male genome.

A papaya-specific gene, papain, used as an endogenous reference gene in qualitative and real-time quantitative PCR detection of transgenic papayas

2008 ◽  
Vol 228 (2) ◽  
pp. 301-309 ◽  
Author(s):  
Wentao Xu ◽  
Weibin Bai ◽  
Feng Guo ◽  
Yunbo Luo ◽  
Yanfang Yuan ◽  
...  
Keyword(s):  
Real Time ◽  
Quantitative Pcr ◽  
Reference Gene ◽  
Pcr Detection ◽  
Specific Gene ◽  
Real Time Quantitative Pcr ◽  
Endogenous Reference Gene ◽  
Endogenous Reference

Development of a rapid detection and quantification method of Karenia mikimotoi by real-time quantitative PCR

Harmful Algae ◽  
2012 ◽  
Vol 17 ◽  
pp. 83-91 ◽  
Author(s):  
Jian Yuan ◽  
Tiezhu Mi ◽  
Yu Zhen ◽  
Zhigang Yu
Keyword(s):  
Real Time ◽  
Quantitative Pcr ◽  
Rapid Detection ◽  
Karenia Mikimotoi ◽  
Real Time Quantitative Pcr ◽  
Quantification Method ◽  
Detection And Quantification

Use of real-time quantitative PCR to investigate root and gall colonisation by co-inoculated isolates of the nematophagous fungusPochonia chlamydosporia

2009 ◽  
Vol 155 (1) ◽  
pp. 143-152 ◽  
Author(s):  
S.D. Atkins ◽  
B. Peteira ◽  
I.M. Clark ◽  
B.R. Kerry ◽  
P.R. Hirsch
Keyword(s):  
Real Time ◽  
Quantitative Pcr ◽  
Real Time Quantitative Pcr

scholarly journals Quantification and Stability of Human Adenoviruses and Polyomavirus JCPyV in Wastewater Matrices

2006 ◽  
Vol 72 (12) ◽  
pp. 7894-7896 ◽  
Author(s):  
Silvia Bofill-Mas ◽  
Nestor Albinana-Gimenez ◽  
Pilar Clemente-Casares ◽  
Ayalkibet Hundesa ◽  
Jesus Rodriguez-Manzano ◽  
...  
Keyword(s):  
Real Time ◽  
Quantitative Pcr ◽  
High Stability ◽  
Wastewater Sludge ◽  
Human Polyomavirus ◽  
Sewage Effluent ◽  
Viral Contamination ◽  
Human Adenoviruses ◽  
Urban Sewage ◽  
Real Time Quantitative Pcr

ABSTRACT Human adenoviruses (HAdV) and human polyomavirus JCPyV have been previously proposed as indicators of fecal viral contamination in the environment. Different wastewater matrices have been analyzed by applying real-time quantitative PCR procedures for the presence, quantity, and stability of a wide diversity of excreted HAdV and JCPyV. High quantities of HAdV and JCPyV were detected in sewage, effluent wastewater, sludge, and biosolid samples. Both viruses showed high stability in urban sewage. These results confirm the suitability of both viruses as indicators of human fecal viral pollution.

IFN-γ induced persistent Chlamydia pneumoniae infection in HL and Mono Mac 6 cells: characterization by real-time quantitative PCR and culture

2004 ◽  
Vol 36 (1) ◽  
pp. 41-50 ◽  
Author(s):  
Laura Mannonen ◽  
Eveline Kamping ◽  
Tuula Penttilä ◽  
Mirja Puolakkainen
Keyword(s):  
Real Time ◽  
Quantitative Pcr ◽  
Chlamydia Pneumoniae ◽  
Real Time Quantitative Pcr ◽  
Ifn Γ

Analysis of Caecal Microbiota in Rats Fed with Genetically Modified Rice by Real-Time Quantitative PCR

2011 ◽  
Vol 76 (1) ◽  
pp. M88-M93 ◽  
Author(s):  
Wentao Xu ◽  
Liting Li ◽  
Jiao Lu ◽  
YunBo Luo ◽  
Ying Shang ◽  
...  
Keyword(s):  
Real Time ◽  
Quantitative Pcr ◽  
Genetically Modified ◽  
Genetically Modified Rice ◽  
Real Time Quantitative Pcr ◽  
Caecal Microbiota

scholarly journals Identification, Detection, and Enumeration of Human Bifidobacterium Species by PCR Targeting the Transaldolase Gene

2002 ◽  
Vol 68 (5) ◽  
pp. 2420-2427 ◽  
Author(s):  
Teresa Requena ◽  
Jeremy Burton ◽  
Takahiro Matsuki ◽  
Karen Munro ◽  
Mary Alice Simon ◽  
...  
Keyword(s):  
Real Time ◽  
16S Rdna ◽  
Quantitative Pcr ◽  
Gene Sequence ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Bifidobacterium Longum ◽  
Bifidobacterium Adolescentis ◽  
Fecal Samples ◽  
Real Time Quantitative Pcr ◽  
Pcr Targeting

ABSTRACT Methods that enabled the identification, detection, and enumeration of Bifidobacterium species by PCR targeting the transaldolase gene were tested. Bifidobacterial species isolated from the feces of human adults and babies were identified by PCR amplification of a 301-bp transaldolase gene sequence and comparison of the relative migrations of the DNA fragments in denaturing gradient gel electrophoresis (DGGE). Two subtypes of Bifidobacterium longum, five subtypes of Bifidobacterium adolescentis, and two subtypes of Bifidobacterium pseudocatenulatum could be differentiated using PCR-DGGE. Bifidobacterium angulatum and B. catenulatum type cultures could not be differentiated from each other. Bifidobacterial species were also detected directly in fecal samples by this combination of PCR and DGGE. The number of species detected was less than that detected by PCR using species-specific primers targeting 16S ribosomal DNA (rDNA). Real-time quantitative PCR targeting a 110-bp transaldolase gene sequence was used to enumerate bifidobacteria in fecal samples. Real-time quantitative PCR measurements of bifidobacteria in fecal samples from adults correlated well with results obtained by culture when either a 16S rDNA sequence or the transaldolase gene sequence was targeted. In the case of samples from infants, 16S rDNA-targeted PCR was superior to PCR targeting the transaldolase gene for the quantification of bifidobacterial populations.

Sign in / Sign up

Export Citation Format

Share Document