Positive detection of mycoplasma contamination by the whole-mount preparation of cell cultures for transmission electron microscopy.

1978 ◽  
Vol 133 (3) ◽  
pp. 1452-1456 ◽  
Author(s):  
G Maul
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Cell Cultures ◽  
Mycoplasma Contamination ◽  
Transmission Electron ◽  
Whole Mount

scholarly journals Development of Sarcocystis falcatula in cell cultures demonstrates that it is different from Sarcocystis neurona

Parasitology ◽  
1999 ◽  
Vol 118 (3) ◽  
pp. 227-233 ◽  
Author(s):  
D. S. LINDSAY ◽  
J. P. DUBEY ◽  
K. M. HORTON ◽  
D. D. BOWMAN
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Cell Cultures ◽  
Residual Body ◽  
Post Inoculation ◽  
Sarcocystis Neurona ◽  
Transmission Electron ◽  
Sarcocystis Falcatula ◽  
Bovine Turbinate

The development of Sarcocystis falcatula merozoites in bovine turbinate (BT) cell cultures is described and compared with development of Sarcocystis neurona merozoites. Merozoites of S. falcatula entered BT cell cultures and increased in size until 3 days post-inoculation when the nucleus of some merozoites developed lobes. Developing schizonts present at 4 days contained a lobed nucleus or appeared multinucleate. A single mature schizont was observed 4 days p.i. Schizonts were numerous 5 and 6 days p.i. Merozoites were produced from blastophores on the schizont. S. neurona merozoites developed to mature schizonts by 3 days p.i. in BT cells and a residual body was often present. Transmission electron microscopy revealed that S. falcatula merozoites possessed more micronemes than did S. neurona merozoites. Our study demonstrates that S. falcatula and S. neurona are not the same parasite.

Transmission and scanning electron microscope observations on the trapping of nematodes by Dactylaria Candida

1977 ◽  
Vol 55 (23) ◽  
pp. 2963-2970 ◽  
Author(s):  
James A. Dowsett ◽  
J. Reid
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Infection Process ◽  
Transmission Electron ◽  
Whole Mount ◽  
Aphelenchus Avenae ◽  
Dactylaria Candida ◽  
Relationship Of ◽  
Scanning Electron

The host–predator relationship of the myceliophagous nematode Aphelenchus avenae Bastian and the predaceous hyphomycete Dactylaria Candida (Nees) Sacc. was investigated using scanning electron microscopy of whole-mount preparations and transmission electron microscopy of ultrathin resin (Spurr) sections. Trapping knobs and knobs with associated hyphae were found to be effective nematode-trapping agents. The ultrastructure of the trapping apparatus and of the nematodes and hyphae during the infection process is reported herein, as is the phenomenon of postinfection 'breakout.' Nonconstricting rings were not observed during this study.

Microscopic observations on the trapping of nematodes by the predaceous fungus Dactylella cionopaga

1984 ◽  
Vol 62 (4) ◽  
pp. 674-679 ◽  
Author(s):  
J. A. Dowsett ◽  
J. Reid ◽  
A. A. Hopkin
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Morphological Development ◽  
Transmission Electron ◽  
Whole Mount ◽  
Aphelenchus Avenae ◽  
The One ◽  
Scanning Electron

The host–predator relationship between the predaceous hyphomycete Dactylella cionopaga Drechs. and the myceliophagous parthenogenetic nematode Aphelenchus avenae Bastian was investigated using interference-contrast and scanning electron microscopy of whole-mount preparations and transmission electron microscopy of ultrathin (Spurr) sections. The one-, two-, three-, or more-celled adhesive trapping structures of this fungus were found to be effective predatory mechanisms at all stages of their morphological development. The trapping structures, mode of capture, and penetration by D. cionopaga were compared with the adhesive knobs, constricting rings, and adhesive hyphal networks of Dactylaria species reported on previously.

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