scholarly journals Phenotypic variations in strain AB1157 cultivars of Escherichia coli from different sources.

1985 ◽  
Vol 164 (3) ◽  
pp. 1381-1382 ◽  
Author(s):  
M Verma ◽  
J B Egan
Keyword(s):  
Escherichia Coli ◽  
Phenotypic Variations ◽  
Different Sources ◽  
Strain Ab1157

scholarly journals EDL933 Strains of Escherichia coli O157 can Demonstrate Genetic Diversity and Differential Adherence to Bovine Recto-Anal Junction Squamous Epithelial Cells

2021 ◽  
Vol 15 (1) ◽  
pp. 129-138
Author(s):  
Raegan S. Hoefler ◽  
Indira T. Kudva
Keyword(s):  
Escherichia Coli ◽  
Wild Type ◽  
Cell Adherence ◽  
O157 Strain ◽  
Shiga Toxins ◽  
The Us ◽  
Strain Type ◽  
Enterocyte Effacement ◽  
Different Sources

Background: Differences between Escherichia coli O157 (O157) strains are well-established with some of these strains being associated with major outbreaks in the US. EDL933 is one such O157 strain that caused a multistate outbreak in 1982 and has since been used as a prototype in various O157-related experiments. Objective: As O157 can readily acquire genetic mutations, we sought to determine if the genetic and phenotypic profiles of EDL933 strains from different sources would be consistent. Methods: We evaluated wild-type O157 strains stocked as EDL933 from three different laboratories, in the strain typing Polymorphic Amplified Typing Sequence (PATS) and the bovine rectal-anal junction squamous epithelial (RSE) cell- and HEp-2 cell- adherence assays. In addition, we also verified if Shiga toxins (Stx), the Locus of Enterocyte Effacement (LEE) or curli fimbriae contributed to the adherence phenotypes observed using mutant and wild-type EDL933 isolates. Results: Our results showed differences in PATS profiles and RSE cell-adherence phenotype, with no influence from the Stx or LEE genes, between EDL933 from different sources. Interestingly, the EDL933 strain that demonstrated the most contrasting diffuse adherence phenotype on RSE cells, EDL933-T, had decreased curli production that may have contributed to this phenotype. Conclusion: Our observations suggest that a comprehensive characterization of bacterial isolates, even if assigned to the same strain type prior to use in experiments, is warranted to ensure consistency and reproducibility of results.

scholarly journals The influence of Escherichia coli strains from different sources and the age of broiler chickens on the development of cellulitis

2001 ◽  
Vol 30 (5) ◽  
pp. 475-478 ◽  
Author(s):  
Larissa C. Johnson ◽  
Sacit F. Bilgili ◽  
Frederick J. Hoerr ◽  
Bruce L. McMurtrey ◽  
Robert A. Norton
Keyword(s):  
Escherichia Coli ◽  
Broiler Chickens ◽  
Different Sources

Chemical and microbiological characterisation of kefir grains

2001 ◽  
Vol 68 (4) ◽  
pp. 639-652 ◽  
Author(s):  
GRACIELA L. GARROTE ◽  
ANALÍA G. ABRAHAM ◽  
GRACIELA L. DE ANTONI
Keyword(s):  
Escherichia Coli ◽  
Lactococcus Lactis ◽  
Leuconostoc Mesenteroides ◽  
Acetic Acid Bacteria ◽  
Fermented Milk ◽  
The Other ◽  
Lactobacillus Kefir ◽  
Kefir Grains ◽  
Inhibitory Power ◽  
Different Sources

Chemical and microbiological composition of four Argentinean kefir grains from different sources as well as characteristics of the corresponding fermented milk were studied. Kefir grains CIDCA AGK1, AGK2 and AGK4 did not show significant differences in their chemical and microbiological composition. In contrast, protein and yeast content of AGK3 was higher than in the other grains. Although grain microflora comprised lactobacilli, lactococcus, acetic acid bacteria and yeast, we found an important difference regarding species. Lactococcus lactis subsp. lactis, Lactobacillus kefir, Lactobacillus plantarum, Acetobacter and Saccharomyces were present in all types of kefir grain. While Leuconostoc mesenteroides was only isolated from grains CIDCA AGK1 and Lactococcus lactis subsp. lactis biovar diacetylactis, Lactobacillus parakefir and Kluyveromyces marxianus were only isolated from CIDCA AGK2 grains. All grains produced acid products with pH between 3·5 and 4·0. The apparent viscosity of AGK1 fermented milk was greater than the product obtained with AGK4. All fermented milks had inhibitory power towards Escherichia coli but AGK1 and AGK2 supernatants were able to halt the bacterial growth for at least 25 h. Grain weight increment in AGK1, AGK2 and AGK3 during growth in milk did not show significant differences. Despite their fermenting activity, AGK4 grains did not increase their weight.

scholarly journals Identification of isoforms of the exocytosis-sensitive phosphoprotein PP63/parafusin in Paramecium tetraurelia and demonstration of phosphoglucomutase activity

1997 ◽  
Vol 323 (1) ◽  
pp. 289-296 ◽  
Author(s):  
Karin HAUSER ◽  
Roland KISSMEHL ◽  
Jürgen LINDER ◽  
Jochen E. SCHULTZ ◽  
Friedrich LOTTSPEICH ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Amino Acid ◽  
Genetic Code ◽  
Polyclonal Antibodies ◽  
Amino Acid Sequences ◽  
Paramecium Tetraurelia ◽  
Universal Genetic Code ◽  
Chicken Muscle ◽  
Similarity Searches ◽  
Different Sources

PP63 (parafusin) is a 63 kDa phosphoprotein which is very rapidly (within 80 ms) dephosphorylated (to P63) during triggered trichocyst exocytosis; this occurs selectively in exocytosis-competent Paramecium tetraurelia strains. In the present work, two cDNAs coding for PP63/parafusin have been isolated, one of which is a new isoform. These isoforms are 99.6% identical and are derived from two different genes. Similarity searches revealed 43-51% identity of the deduced amino acid sequences with known phosphoglucomutases from yeast and mammals. The sequences of two proteolytic peptides obtained from PP63/parafusin isolated from Paramecium are identical to parts of the amino acid sequence deduced from the major cDNA. The major cDNA was mutated from the macronuclear ciliate genetic code into the universal genetic code and expressed in Escherichia coli. The recombinant protein shows the same biochemical and immunological characteristics as the (P)P63/parafusin originally isolated from Paramecium. It has the same specific phosphoglucomutase activity as phosphoglucomutase from chicken muscle. We also show that recombinant P63-1/parafusin 1 is a substrate of an endogenous casein kinase from Paramecium, as is the originally isolated P63/parafusin. Polyclonal antibodies against recombinant P63-1/parafusin 1 were raised which recognized phosphoglucomutases from different sources. Thus we show that PP63/parafusin and phosphoglucomutase in Paramecium are identical.

scholarly journals Virulence factors, prevalence and potential transmission of extraintestinal pathogenic Escherichia coli isolated from different sources: recent reports

Gut Pathogens ◽  
2019 ◽  
Vol 11 (1) ◽  
Author(s):  
Jolanta Sarowska ◽  
Bozena Futoma-Koloch ◽  
Agnieszka Jama-Kmiecik ◽  
Magdalena Frej-Madrzak ◽  
Marta Ksiazczyk ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Virulence Factors ◽  
Pathogenic Escherichia Coli ◽  
Different Sources
Sign in / Sign up

Export Citation Format

Share Document