scholarly journals Expression of degradative genes of Pseudomonas putida in Caulobacter crescentus.

1987 ◽  
Vol 169 (7) ◽  
pp. 2962-2966 ◽  
Author(s):  
D K Chatterjee ◽  
P Chatterjee
Keyword(s):  
Pseudomonas Putida ◽  
Caulobacter Crescentus

scholarly journals Establishment of Oxidative d-Xylose Metabolism in Pseudomonas putida S12

2009 ◽  
Vol 75 (9) ◽  
pp. 2784-2791 ◽  
Author(s):  
Jean-Paul Meijnen ◽  
Johannes H. de Winde ◽  
Harald J. Ruijssenaars
Keyword(s):  
Pseudomonas Putida ◽  
Caulobacter Crescentus ◽  
Glucose Dehydrogenase ◽  
Dry Weight ◽  
Maximum Growth ◽  
Maximum Growth Rate ◽  
Xylose Utilization ◽  
Catabolic Pathway ◽  
Xylose Metabolism ◽  
Semialdehyde Dehydrogenase

ABSTRACT The oxidative d-xylose catabolic pathway of Caulobacter crescentus, encoded by the xylXABCD operon, was expressed in the gram-negative bacterium Pseudomonas putida S12. This engineered transformant strain was able to grow on d-xylose as a sole carbon source with a biomass yield of 53% (based on g [dry weight] g d-xylose−1) and a maximum growth rate of 0.21 h−1. Remarkably, most of the genes of the xylXABCD operon appeared to be dispensable for growth on d-xylose. Only the xylD gene, encoding d-xylonate dehydratase, proved to be essential for establishing an oxidative d-xylose catabolic pathway in P. putida S12. The growth performance on d-xylose was, however, greatly improved by coexpression of xylXA, encoding 2-keto-3-deoxy-d-xylonate dehydratase and α-ketoglutaric semialdehyde dehydrogenase, respectively. The endogenous periplasmic glucose dehydrogenase (Gcd) of P. putida S12 was found to play a key role in efficient oxidative d-xylose utilization. Gcd activity not only contributes to d-xylose oxidation but also prevents the intracellular accumulation of toxic catabolic intermediates which delays or even eliminates growth on d-xylose.

scholarly journals Spatial control of gene expression in flies using bacterially derived binary transactivation systems

2020 ◽  
Author(s):  
Stephanie Gamez ◽  
Luis C. Vesga ◽  
Stelia C. Mendez-Sanchez ◽  
Omar S. Akbari
Keyword(s):  
Gene Expression ◽  
Pseudomonas Putida ◽  
Caulobacter Crescentus ◽  
Streptomyces Coelicolor ◽  
Molecular Genetic ◽  
Genetic Studies ◽  
Control Of Gene Expression ◽  
Multiple Systems

AbstractControlling gene expression is an instrumental tool for biotechnology, as it enables the dissection of gene function, affording precise spatial-temporal resolution. To generate this control, binary transactivational systems have been used employing a modular activator consisting of a DNA binding domain(s) fused to activation domain(s). For fly genetics, many binary transactivational systems have been exploited in vivo; however as the study of complex problems often requires multiple systems that can be used in parallel, there is a need to identify additional bipartite genetic systems. To expand this molecular genetic toolbox, we tested multiple bacterially-derived binary transactivational systems in Drosophila melanogaster including the p-CymR operon from Pseudomonas putida, PipR operon from Streptomyces coelicolor, TtgR operon from Pseudomonas putida, and the VanR operon from Caulobacter crescentus. Our work provides the first characterization of these systems in an animal model in vivo. For each system we demonstrate robust tissue-specific spatial transactivation of reporter gene expression, enabling future studies to exploit these transactivational systems for molecular genetic studies.

Enzimaktivitások és a fluoreszkáló pszeudomonasz csíraszámok változása a fehér lóhere (Trifolium repens L.) rizoszférájában sókezelés (NaCl) hatására

2004 ◽  
Vol 53 (3-4) ◽  
pp. 367-376 ◽  
Author(s):  
A. A. Khalif ◽  
H. Abdorhim ◽  
Hosam E. H. T. Bayoumi ◽  
Anna Füzy ◽  
Mihály Kecskés
Keyword(s):  
Pseudomonas Putida ◽  
Trifolium Repens ◽  
Trifolium Repens L

Üvegházi körülmények között savanyú barna erdotalajban nevelt fehér here (Trifolium repens L.) növények rizoszférájának sókezelés hatására bekövetkezo változását ellenoriztük. Megvizsgáltuk a különbözo sókoncentrációknak (0, 0,2, 0,4, 0,6 és 0,8 tömeg %) a baktériumnépesség összetételére és a különbözo talajenzimek aktivitására gyakorolt hatását.  Megállapítottuk, hogy a talaj sótartalma közvetlenül befolyásolta a rizoszférában található fluoreszkáló pszeudomonaszok csíraszámát. A legsurubb populáció a 0,2% NaCl-ot tartalmazó talajban volt mérheto, ahol a fluoreszkáló pszeudomonaszok között a Pseudomonas putida és a P. fluorescens fordultak elo a legnagyobb számban. A pszeudomonaszok ily módon jól tolerálják a talaj magas NaCl-tartalmát, és gyökérkolonizáló tevékenységet képesek kifejteni a magas NaCl-tartalmú talajban is. A sókoncentráció növelésével kezdetben (a 0,2-0,4%-os tartományban) jelentosen növekedett a dehidrogenáz, kataláz, és ureáz enzimek aktivitása. A proteáz enzimek aktivitásmaximuma a 0,1-0,2% NaCl-koncentráció tartományba esett. A 0,4%-nál magasabb koncentrációkban a kontrollhoz hasonló mértékure csökkent mind a négy enzim aktivitása, és a baktériumok száma is. A foszfatáz- és a b-glükozidáz-tevékenység viszont a NaCl-dózis növelése következtében a koncentrációval arányosan, jelentosen csökkent a kontrollhoz viszonyítva.  Feltételezésünk szerint az enzimaktivitások változását is a sókezelés hatására bekövetkezo mikrobióta összetételének megváltozása okozta.

Exemplar Abstract for Pseudomonas putida (Trevisan 1889) Migula 1895 (Approved Lists 1980).

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Dorothea Taylor ◽  
George M Garrity
Keyword(s):  
Pseudomonas Putida

Effect of rhizobacteria strains on the induction of resistance in barley genotypes against Cochliobolus sativus

2020 ◽  
Vol 13 (2) ◽  
pp. 83-92 ◽  
Author(s):  
A. Adam
Keyword(s):  
Plant Growth ◽  
Pseudomonas Putida ◽  
Growth Parameters ◽  
Disease Incidence ◽  
Dry Weight ◽  
Cochliobolus Sativus ◽  
Resistance Level ◽  
Wet Weight ◽  
Number Of Leaves ◽  
Barley Genotypes

SummaryEnhancement of the resistance level in plants by rhizobacteria has been proven in several pathosystems. This study investigated the ability of four rhizobacteria strains (Pseudomonas putida BTP1 and Bacillus subtilis Bs2500, Bs2504 and Bs2508) to promote the growth in three barley genotypes and protect them against Cochliobolus sativus. Our results demonstrated that all tested rhizobacteria strains had a protective effect on barley genotypes Arabi Abiad, Banteng and WI2291. However, P. putida BTP1 and B. subtilis Bs2508 strains were the most effective as they reduced disease incidence by 53 and 38% (mean effect), respectively. On the other hand, there were significant differences among the rhizobacteria-treated genotypes on plant growth parameters, such as wet weight, dry weight, plant height and number of leaves. Pseudomonas putida BTP1 strain was the most effective as it significantly increased plant growth by 15-32%. In addition, the susceptible genotypes Arabi Abiad and WI2291 were the most responsive to rhizobacteria. This means that these genotypes have a high potential for increase of their resistance against the pathogen and enhancement of plant growth after the application of rhizobacteria. Consequently, barley seed treatment with the tested rhizobacteria could be considered as an effective biocontrol method against C. sativus.

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