scholarly journals Klebsiella pneumoniae origin of replication (oriC) is not active in Caulobacter crescentus, Pseudomonas putida, and Rhodobacter sphaeroides.

1988 ◽  
Vol 170 (8) ◽  
pp. 3774-3777 ◽  
Author(s):  
E A O'Neill ◽  
R A Bender
Keyword(s):  
Klebsiella Pneumoniae ◽  
Pseudomonas Putida ◽  
Rhodobacter Sphaeroides ◽  
Caulobacter Crescentus ◽  
Origin Of Replication

scholarly journals The Sinorhizobium meliloti chromosomal origin of replication

Microbiology ◽  
2006 ◽  
Vol 152 (2) ◽  
pp. 443-455 ◽  
Author(s):  
Christopher D. Sibley ◽  
Shawn R. MacLellan ◽  
Turlough Finan
Keyword(s):  
Sinorhizobium Meliloti ◽  
Caulobacter Crescentus ◽  
Root Nodules ◽  
Replication Initiation ◽  
Cell Phenotype ◽  
Origin Of Replication ◽  
Mobility Shift ◽  
Autonomous Replication ◽  
Chromosomal Origin ◽  
Mobility Shift Assay

The predicted chromosomal origin of replication (oriC) from the alfalfa symbiont Sinorhizobium meliloti is shown to allow autonomous replication of a normally non-replicating plasmid within S. meliloti cells. This is the first chromosomal replication origin to be experimentally localized in the Rhizobiaceae and its location, adjacent to hemE, is the same as for oriC in Caulobacter crescentus, the only experimentally characterized alphaproteobacterial oriC. Using an electrophoretic mobility shift assay and purified S. meliloti DnaA replication initiation protein, binding sites for DnaA were mapped in the S. meliloti oriC region. Mutations in these sites eliminated autonomous replication. S. meliloti that expressed DnaA from a plasmid lac promoter was observed to form pleomorphic filamentous cells, suggesting that cell division was perturbed. Interestingly, this cell phenotype is reminiscent of differentiated bacteroids found inside plant cells in alfalfa root nodules.

scholarly journals Establishment of Oxidative d-Xylose Metabolism in Pseudomonas putida S12

2009 ◽  
Vol 75 (9) ◽  
pp. 2784-2791 ◽  
Author(s):  
Jean-Paul Meijnen ◽  
Johannes H. de Winde ◽  
Harald J. Ruijssenaars
Keyword(s):  
Pseudomonas Putida ◽  
Caulobacter Crescentus ◽  
Glucose Dehydrogenase ◽  
Dry Weight ◽  
Maximum Growth ◽  
Maximum Growth Rate ◽  
Xylose Utilization ◽  
Catabolic Pathway ◽  
Xylose Metabolism ◽  
Semialdehyde Dehydrogenase

ABSTRACT The oxidative d-xylose catabolic pathway of Caulobacter crescentus, encoded by the xylXABCD operon, was expressed in the gram-negative bacterium Pseudomonas putida S12. This engineered transformant strain was able to grow on d-xylose as a sole carbon source with a biomass yield of 53% (based on g [dry weight] g d-xylose−1) and a maximum growth rate of 0.21 h−1. Remarkably, most of the genes of the xylXABCD operon appeared to be dispensable for growth on d-xylose. Only the xylD gene, encoding d-xylonate dehydratase, proved to be essential for establishing an oxidative d-xylose catabolic pathway in P. putida S12. The growth performance on d-xylose was, however, greatly improved by coexpression of xylXA, encoding 2-keto-3-deoxy-d-xylonate dehydratase and α-ketoglutaric semialdehyde dehydrogenase, respectively. The endogenous periplasmic glucose dehydrogenase (Gcd) of P. putida S12 was found to play a key role in efficient oxidative d-xylose utilization. Gcd activity not only contributes to d-xylose oxidation but also prevents the intracellular accumulation of toxic catabolic intermediates which delays or even eliminates growth on d-xylose.

scholarly journals Conserved Gene Cluster at Replication Origins of the α-Proteobacteria Caulobacter crescentus andRickettsia prowazekii

2001 ◽  
Vol 183 (5) ◽  
pp. 1824-1829 ◽  
Author(s):  
Ann Karen C. Brassinga ◽  
Rania Siam ◽  
Gregory T. Marczynski
Keyword(s):  
Gene Cluster ◽  
Genome Sequence ◽  
Replication Origin ◽  
Caulobacter Crescentus ◽  
Origin Of Replication ◽  
Replication Origins ◽  
Rickettsia Prowazekii ◽  
Putative Origin ◽  
Conserved Gene

ABSTRACT A 30-kb region surrounding the replication origin inCaulobacter crescentus was analyzed. Comparison to the genome sequence of another α-proteobacterium, Rickettsia prowazekii, revealed a conserved cluster of genes (RP001,hemE, hemH, and RP883) that overlaps the established origin of replication in C. crescentus and the putative origin of replication in R. prowazekii. The genes flanking this cluster differ between these two organisms. We therefore propose that this conserved gene cluster can be used to identify the origin of replication in other α-proteobacteria.

scholarly journals In Vivo Imaging of the Segregation of the 2 Chromosomes and the Cell Division Proteins of Rhodobacter sphaeroides Reveals an Unexpected Role for MipZ

mBio ◽  
2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Nelly Dubarry ◽  
Clare R. Willis ◽  
Graeme Ball ◽  
Christian Lesterlin ◽  
Judith P. Armitage
Keyword(s):  
Cell Division ◽  
Chromosome Segregation ◽  
Rhodobacter Sphaeroides ◽  
Caulobacter Crescentus ◽  
Bacterial Species ◽  
Chromosome 1 ◽  
Chromosome 2 ◽  
Content Type ◽  
Ftsz Ring ◽  
Chromosome Duplication

ABSTRACT Coordinating chromosome duplication and segregation with cell division is clearly critical for bacterial species with one chromosome. The precise choreography required is even more complex in species with more than one chromosome. The alpha subgroup of bacteria contains not only one of the best-studied bacterial species, Caulobacter crescentus, but also several species with more than one chromosome. Rhodobacter sphaeroides is an alphaproteobacterium with two chromosomes, but, unlike C. crescentus, it divides symmetrically rather than buds and lacks the complex CtrA-dependent control mechanism. By examining the Ori and Ter regions of both chromosomes and associated ParA and ParB proteins relative to cell division proteins FtsZ and MipZ, we have identified a different pattern of chromosome segregation and cell division. The pattern of chromosome duplication and segregation resembles that of Vibrio cholerae, not that of Agrobacterium tumefaciens, with duplication of the origin and terminus regions of chromosome 2 controlled by chromosome 1. Key proteins are localized to different sites compared to C. crescentus. OriC1 and ParB1 are localized to the old pole, while MipZ and FtsZ localize to the new pole. Movement of ParB1 to the new pole following chromosome duplication releases FtsZ, which forms a ring at midcell, but, unlike reports for other species, MipZ monomers do not form a gradient but oscillate between poles, with the nucleotide-bound monomer and the dimer localizing to midcell. MipZ dimers form a single ring (with a smaller diameter) close to the FtsZ ring at midcell and constrict with the FtsZ ring. Overproduction of the dimer form results in filamentation, suggesting that MipZ dimers are regulating FtsZ activity and thus septation. This is an unexpected role for MipZ and provides a new model for the integration of chromosome segregation and cell division. IMPORTANCE Cell division has to be coordinated with chromosome segregation to ensure the stable inheritance of genetic information. We investigated this coordination in the multichromosome bacterium Rhodobacter sphaeroides. By examining the origin and terminus regions of the two chromosomes, the ParA-like ATPase MipZ and FtsZ, we showed that chromosome 1 appears to be the “master” chromosome connecting DNA segregation and cell division, with MipZ being critical for coordination. MipZ shows an unexpected localization pattern, with MipZ monomers interacting with ParB of the chromosome 1 at the cell poles whereas MipZ dimers colocalize with FtsZ at midcell during constriction, both forming dynamic rings. These data suggest that MipZ has roles in R. sphaeroides in both controlling septation and coordinating chromosome segregation with cell division.

scholarly journals A comparison of the Caulobacter NA1000 and K31 genomes reveals extensive genome rearrangements and differences in metabolic potential

Open Biology ◽  
2014 ◽  
Vol 4 (10) ◽  
pp. 140128 ◽  
Author(s):  
Kurt Ash ◽  
Theta Brown ◽  
Tynetta Watford ◽  
LaTia E. Scott ◽  
Craig Stephens ◽  
...  
Keyword(s):  
Amino Acid ◽  
Doubling Time ◽  
Amino Acid Sequence Identity ◽  
Caulobacter Crescentus ◽  
Careful Analysis ◽  
Origin Of Replication ◽  
Metabolic Potential ◽  
Small Indels ◽  
Sequence Identity ◽  
Large Plasmids

The genus Caulobacter is found in a variety of habitats and is known for its ability to thrive in low-nutrient conditions. K31 is a novel Caulobacter isolate that has the ability to tolerate copper and chlorophenols, and can grow at 4°C with a doubling time of 40 h. K31 contains a 5.5 Mb chromosome that codes for more than 5500 proteins and two large plasmids (234 and 178 kb) that code for 438 additional proteins. A comparison of the K31 and the Caulobacter crescentus NA1000 genomes revealed extensive rearrangements of gene order, suggesting that the genomes had been randomly scrambled. However, a careful analysis revealed that the distance from the origin of replication was conserved for the majority of the genes and that many of the rearrangements involved inversions that included the origin of replication. On a finer scale, numerous small indels were observed. K31 proteins involved in essential functions shared 80–95% amino acid sequence identity with their C. crescentus homologues, while other homologue pairs tended to have lower levels of identity. In addition, the K31 chromosome contains more than 1600 genes with no homologue in NA1000.

scholarly journals Spatial control of gene expression in flies using bacterially derived binary transactivation systems

2020 ◽  
Author(s):  
Stephanie Gamez ◽  
Luis C. Vesga ◽  
Stelia C. Mendez-Sanchez ◽  
Omar S. Akbari
Keyword(s):  
Gene Expression ◽  
Pseudomonas Putida ◽  
Caulobacter Crescentus ◽  
Streptomyces Coelicolor ◽  
Molecular Genetic ◽  
Genetic Studies ◽  
Control Of Gene Expression ◽  
Multiple Systems

AbstractControlling gene expression is an instrumental tool for biotechnology, as it enables the dissection of gene function, affording precise spatial-temporal resolution. To generate this control, binary transactivational systems have been used employing a modular activator consisting of a DNA binding domain(s) fused to activation domain(s). For fly genetics, many binary transactivational systems have been exploited in vivo; however as the study of complex problems often requires multiple systems that can be used in parallel, there is a need to identify additional bipartite genetic systems. To expand this molecular genetic toolbox, we tested multiple bacterially-derived binary transactivational systems in Drosophila melanogaster including the p-CymR operon from Pseudomonas putida, PipR operon from Streptomyces coelicolor, TtgR operon from Pseudomonas putida, and the VanR operon from Caulobacter crescentus. Our work provides the first characterization of these systems in an animal model in vivo. For each system we demonstrate robust tissue-specific spatial transactivation of reporter gene expression, enabling future studies to exploit these transactivational systems for molecular genetic studies.

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