scholarly journals Establishment of Oxidative d-Xylose Metabolism in Pseudomonas putida S12

2009 ◽  
Vol 75 (9) ◽  
pp. 2784-2791 ◽  
Author(s):  
Jean-Paul Meijnen ◽  
Johannes H. de Winde ◽  
Harald J. Ruijssenaars
Keyword(s):  
Pseudomonas Putida ◽  
Caulobacter Crescentus ◽  
Glucose Dehydrogenase ◽  
Dry Weight ◽  
Maximum Growth ◽  
Maximum Growth Rate ◽  
Xylose Utilization ◽  
Catabolic Pathway ◽  
Xylose Metabolism ◽  
Semialdehyde Dehydrogenase

ABSTRACT The oxidative d-xylose catabolic pathway of Caulobacter crescentus, encoded by the xylXABCD operon, was expressed in the gram-negative bacterium Pseudomonas putida S12. This engineered transformant strain was able to grow on d-xylose as a sole carbon source with a biomass yield of 53% (based on g [dry weight] g d-xylose−1) and a maximum growth rate of 0.21 h−1. Remarkably, most of the genes of the xylXABCD operon appeared to be dispensable for growth on d-xylose. Only the xylD gene, encoding d-xylonate dehydratase, proved to be essential for establishing an oxidative d-xylose catabolic pathway in P. putida S12. The growth performance on d-xylose was, however, greatly improved by coexpression of xylXA, encoding 2-keto-3-deoxy-d-xylonate dehydratase and α-ketoglutaric semialdehyde dehydrogenase, respectively. The endogenous periplasmic glucose dehydrogenase (Gcd) of P. putida S12 was found to play a key role in efficient oxidative d-xylose utilization. Gcd activity not only contributes to d-xylose oxidation but also prevents the intracellular accumulation of toxic catabolic intermediates which delays or even eliminates growth on d-xylose.

Carboxymethylhydroxymuconic semialdehyde dehydrogenase in the 4-hydroxyphenylacetate catabolic pathway of Pseudomonas putida

1986 ◽  
Vol 64 (12) ◽  
pp. 1288-1293 ◽  
Author(s):  
Josefa M. Alonso ◽  
Amando Garrido-Pertierra
Keyword(s):  
Pseudomonas Putida ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Gel Filtration ◽  
Competitive Inhibitor ◽  
Catabolic Pathway ◽  
Ph Optimum ◽  
Semialdehyde Dehydrogenase ◽  
Standard Assay ◽  
Assay Conditions

5-Carboxymethyl-2-hydroxymuconic semialdehyde (CHMSA) dehydrogenase in the 4-hydroxyphenylacetate meta-cleavage pathway was purified from Pseudomonas putida by gel filtration, anion-exchange, and affinity chromatographies. Sodium dodecyl sulfate – polyacrylamide gel electrophoresis analysis suggested an approximate tetrameric molecular weight of 200 000. The purified enzyme showed a pH optimum at 7.8. The temperature–activity relationship for the enzyme from 27 to 45 °C showed broken Arrhenius plots with an inflexion at 36–37 °C. Under standard assay conditions, the enzyme acted preferentially with NAD. It could also catalyze the reduction with NADP (which had a higher Km), at 18% of the rate observed for NAD. The following kinetic parameters were found: Km(NAD) = 20.0 ± 3.6 μM, Km(CHMSA) = 8.5 ± 1.8 μM, and Kd(enzyme–NAD complex) = 7.8 ± 2.0 μM. The product NADH acted as a competitive inhibitor against NAD.

Field Determination of the Critical Nutrient Concentrations for Cladophora in Streams

1976 ◽  
Vol 33 (1) ◽  
pp. 85-92 ◽  
Author(s):  
S. L. Wong ◽  
B. Clark
Keyword(s):  
Plant Tissue ◽  
Stream Water ◽  
Dry Weight ◽  
Maximum Growth ◽  
Maximum Growth Rate ◽  
Nutrient Concentrations ◽  
P Concentration ◽  
P Content ◽  
Waste Load ◽  
Total P

Many streams in southern Ontario experience excessive seasonal growth of aquatic plants such as Cladophora and Potamogeton. A direct relation, with a regression coefficient of 0.87, was observed between ambient P concentration in the water and P content of plant tissue in six rivers. Critical or growth controlling total P concentration of 60 μg/liter in stream water and 1.6 mg/gram dry weight in plant tissue were determined. Unlike P, no significant correlation was observed between N content of plant tissue and N concentration in water. The correlation of total P with plant growth can be used to estimate the waste load which would result in maximum growth rate of Cladophora.

Growth Rates and Nitrogen and Carbohydrate Contents of Juvenile Clams, Saxidomus giganteus, Fed Three Species of Algae

1973 ◽  
Vol 30 (12) ◽  
pp. 1825-1830 ◽  
Author(s):  
P. R. Walne
Keyword(s):  
Isochrysis Galbana ◽  
Live Weight ◽  
Dry Weight ◽  
Maximum Growth ◽  
Condition Index ◽  
Maximum Growth Rate ◽  
Experimental Conditions ◽  
Total Dry Weight ◽  
Tetraselmis Chui ◽  
Saxidomus Giganteus

Estimates were made of the increase in live and dry weights and in the content of ash, organic matter, carbohydrate (as glucose), and nitrogen when juveniles of the clam Saxidomus giganteus were fed for 21 days at various concentrations of Tetraselmis chui, Isochrysis galbana, and Phaeodactylum tricornutum. The condition index (organic weight as a percent of total dry weight) increased in all the experimental conditions with a mixture of I. galbana and T. chui, yielding a significantly higher index than either species on its own. The relative gain in glucose was greater than the increase in live weight or in nitrogen. The increase in live weight was such that the nigrogen content was approximately constant at 6–7 μgN/mg live weight irrespective of the feeding conditions. The N:glucose ratio decreased with increasing concentrations of I. galbana and P. tricornutum, and at the higher concentrations, where the maximum growth rate of clams occurred, the ratios were lower than in any of the clams fed on T. chui.

scholarly journals Evaluation of Marine Synechococcus for an Algal Biorefinery in Arid Regions

Energies ◽  
2019 ◽  
Vol 12 (12) ◽  
pp. 2233 ◽  
Author(s):  
Tomasz Bochenski ◽  
Tanmay Chaturvedi ◽  
Mette Hedegaard Thomsen ◽  
Jens Ejbye Schmidt
Keyword(s):  
Sodium Hydroxide ◽  
United Arab Emirates ◽  
Biomass Concentration ◽  
Local Strain ◽  
Dry Weight ◽  
Maximum Growth ◽  
Maximum Growth Rate ◽  
Cell Disruption ◽  
Arid Environments ◽  
Wide Range

Implementing microalgae biorefinery in arid environments requires utilization of strains that can grow at high temperatures (above 28 °C) and salinity levels (above 30 ppt). In this study, we investigate the newly isolated seawater strain, Synechococcus, native to the United Arab Emirates, and evaluate its value as a perspective organism for cultivation (for fuel and bio-products) in regions with freshwater scarcity. The strain displayed tolerance to a wide range of temperature (22–37 °C) and salinity (20–41 ppt), with maximum biomass concentration of 0.72 g L−1 and a maximum growth rate of 82 mg L−1 d−1 at 25 °C and 33 ppt salinity. Lipids accumulation reached up to 26% of dry weight in nitrogen-depleted conditions (with 1.8 mM of nitrates addition to the media), whereas protein content exceeded 50% dry weight. In this study, harvesting is investigated using three chemical agents: Ferric chloride, sodium hydroxide, and chitosan. Cell disruption is analyzed for four distinct treatments: Enzymatic, alkaline, ultrasonic, and hydrothermal. Among tested methods, flocculation with sodium hydroxide and ultrasonication were found to be the most efficient techniques for harvesting and cell disruption, respectively. The growth characteristics of the local strain and the potential to derive protein and lipids from it makes it a promising biomass in a biorefinery context.

Growth of a Methane-Utilizing Mixed Culture HD6T on Methanol and Poly-β-Hydroxybutyrate Biosynthesis

2010 ◽  
Vol 160-162 ◽  
pp. 171-175 ◽  
Author(s):  
Jing Dong ◽  
Jia Ying Xin ◽  
Ying Xin Zhang ◽  
Lin Lin Chen ◽  
Hong Ye Liang ◽  
...  
Keyword(s):  
Mixed Culture ◽  
Mineral Salt Medium ◽  
Biomass Concentration ◽  
Dry Weight ◽  
Maximum Growth ◽  
Maximum Growth Rate ◽  
Production Costs ◽  
Lag Phase ◽  
Phase Duration ◽  
Phb Production

Methane-utilizing mixed culture HD6T was successfully cultivated in a brief non-sterile process using methanol as a sole carbon and energy source for the production of poly-β-hydroxybutyrate(PHB). Shake-flask experiments showed HD6T could grow well in the mineral salt medium with the addition of methanol exposed to the air directly. This non-sterile process and the use of cheap substrates (methanol) can reduce the production costs of PHB. It was found that HD6T grew better and PHB production in a more effective way with an initial liquid methanol concentration of 0.15%(v/v).The lag phase duration, the maximum growth rate, the biomass concentration and the PHB yield, for the optimal conditions were, respectively, 12.03h, 0.04h-1(OD600), 1.54g/l(dry weight), 0.424g/l(dry weight). Methane-utilizing mixed culture HD6T appears to be a promising organism for PHB production.

scholarly journals Engineering of a Xylose Metabolic Pathway in Rhodococcus Strains

2012 ◽  
Vol 78 (16) ◽  
pp. 5483-5491 ◽  
Author(s):  
Xiaochao Xiong ◽  
Xi Wang ◽  
Shulin Chen
Keyword(s):  
Carbon Source ◽  
Metabolic Pathway ◽  
Neutral Lipids ◽  
Shuttle Vector ◽  
Dry Weight ◽  
Rhodococcus Opacus ◽  
Xylose Utilization ◽  
Organic Substrates ◽  
Xylose Metabolism ◽  
Content Type

ABSTRACTThe two metabolically versatile actinobacteriaRhodococcus opacusPD630 andR. jostiiRHA1 can efficiently convert diverse organic substrates into neutral lipids mainly consisting of triacylglycerol (TAG), the precursor of energy-rich hydrocarbon. Neither, however, is able to utilize xylose, the important component present in lignocellulosic biomass, as the carbon source for growth and lipid accumulation. In order to broaden their substrate utilization range, the metabolic pathway ofd-xylose utilization was introduced into these two strains. This was accomplished by heterogenous expression of two well-selected genes,xylA, encoding xylose isomerase, andxylB, encoding xylulokinase fromStreptomyces lividansTK23, under the control of thetacpromoter with anEscherichia coli-Rhodococcusshuttle vector. The recombinantR. jostiiRHA1 bearingxylAcould grow on xylose as the sole carbon source, and additional expression ofxylBfurther improved the biomass yield. The recombinant could consume both glucose and xylose in the sugar mixture, although xylose metabolism was still affected by the presence of glucose. The xylose metabolic pathway was also introduced into the high-lipid-producing strainR. opacusPD630 by expression ofxylAandxylB. Under nitrogen-limited conditions, the fatty acid composition was determined, and lipid produced from xylose by recombinants ofR. jostiiRHA1 andR. opacusPD630 carryingxylAandxylBrepresented up to 52.5% and 68.3% of the cell dry weight (CDW), respectively. This work demonstrates that it is feasible to produce lipid from the sugars, including xylose, derived from renewable feedstock by genetic modification of rhodococcus strains.

scholarly journals Effect of Salinity Difference on Lipid Content from Chaetoceros muelleri on Continuous Reactors

2019 ◽  
Vol 7 (1) ◽  
pp. 3-10
Author(s):  
Arif Rahmadi ◽  
Yeni Mulyani ◽  
Muhammad Wahyudin Lewaru
Keyword(s):  
Lipid Content ◽  
Dry Weight ◽  
Maximum Growth ◽  
Maximum Growth Rate ◽  
Salinity Treatment ◽  
Chaetoceros Muelleri ◽  
Marine Microbiology ◽  
Randomized Design ◽  
Total Dry Weight ◽  
Salinity Difference

Chaetoceros muelleri is a microalgae class of Bacillariophyta (diatom) which is generally only used as feeds for fishes and shellfish larvae. Nevertheless, the biochemical content of this species is quite high and has the potential to be developed. This research aims to explain the effect of different salinity on the growth and lipid content of Chaetoceros muelleri cultured in a continuous photobioreactor. This research was carried out in August 2018 - February 2019. The research was conducted at the Laboratory of Marine Microbiology and the Laboratory of Bioprocess and Bioprospection of Natural Materials, Faculty of Fisheries and Marine Sciences, Padjadjaran University. The samples of Chaetoceros muelleri isolates were obtained from the Jepara Brackish Water Aquaculture Center. The methods used for the study was a ‘Completely Randomized Design’ (CRD) with four treatments. The salinity used is 15, 25, 35 and 45 ppt. The main parameters observed were growth and lipid content, while the supporting parameters were temperature, and pH. The results of this study showed that the highest lipid content was a salinity treatment of 35 ppt with a value of 25.37% of total dry weight obtained at the end of the culture. Based on growth, the highest density occurred in 25 ppt salinity with a maximum density of 3.80 ± 0.49 x 106 cells. ml-1 and maximum growth rate of 0.36 ± 0.008 div. day-1

scholarly journals Variation in the fatty acid profiles of two cold water diatoms grown under different temperature, light, and nutrient regimes

2021 ◽  
Author(s):  
Kristian Spilling ◽  
Jukka Seppälä ◽  
Dagmar Schwenk ◽  
Heiko Rischer ◽  
Timo Tamminen
Keyword(s):  
Nutrient Limitation ◽  
Cold Water ◽  
Photosynthetic Apparatus ◽  
Dry Weight ◽  
Maximum Growth ◽  
Maximum Growth Rate ◽  
Omega 3 Fatty Acids ◽  
Omega 3 ◽  
Wide Range ◽  
Different Temperatures

AbstractThere is a growing demand for marine omega-3 fatty acids (FAs) that is produced in high amounts by some microalgae. Here we determined the FA profiles of two cold water adapted diatoms, Chaetoceros wighamii and Thalassiosira baltica. The cultures were acclimated to different temperatures (3, 7, 11, 15, and 19 °C) and irradiance (20, 40, 130, and 450 μmol photons m−2 s−1) and the FA profiles were determined in exponential and stationary growth phases, the latter induced by different nutrient limitation (N, P, and Si). The maximum growth rate was obtained by both species at 11 °C, ≥ 130 μmol photons m−2 s−1 and was 0.8 day−1 and 0.6 day−1 for C. wighamii and T. baltica, respectively. Both species contained relatively high amounts of eicosapentaenoic acid (EPA). Thalassiosira baltica accumulated maximally ~ 30 mg EPA g−1 ash-free dry weight (AFDW) under Si-limitation. The content of docosahexaenoic acid (DHA) was lower, reaching up to 4 mg DHA g−1 AFDW in T. baltica. The concentration of EPA correlated positively with the chlorophyll a:carbon ratio, suggesting that it is bound to membranes in the photosynthetic apparatus and the EPA content in T. baltica was high enough to consider it as a potent candidate for cultivation under cold (< 15 °C) conditions. Covering a wide range of environmental conditions, the strongest differentiation in FA profiles was observed between the species with the growth phase/nutrient limitation pattern as the second most important driver of the FA composition.

scholarly journals Growth rate and haploidization of Aspergillus niger on medium containing p-fluorophenylalanine

1968 ◽  
Vol 12 (3) ◽  
pp. 305-315 ◽  
Author(s):  
Pol Lhoas
Keyword(s):  
Growth Rate ◽  
Growth Rates ◽  
Growth Curves ◽  
Dry Weight ◽  
Maximum Growth ◽  
Maximum Growth Rate ◽  
Complete Medium ◽  
Acid Analogue ◽  
Mean Size ◽  
The Mean

1. The comparison of the dry weight of thin layer haploid and diploid colonies of A. niger on complete medium and complete medium supplemented with p-fluoro-phenylalanine led to the conclusion that there is a difference in growth rates of hyphae under these different conditions.2. The growth curves of the same strains on both media were established. On complete medium, haploids and diploid show a growth rate increasing linearly for about 20 h after germination and reaching a maximum which is then maintained. On p-fluorophenylalanine, the haploids show a similar curve, although the maximum growth rate reached and maintained is about half that on complete medium; for the diploid, however, the maximum is less than the corresponding one in the haploid and, once this maximum has been reached, the growth rate goes down linearly to a very low value which is then maintained.3. The cytological study of the hyphal tip cell showed, in the presence of the amino acid analogue, a reduction of the mean size of the diploid nuclei together with an increase of the number of nuclear fragments. This explains the growth rates observed and is accepted as a confirmation that p-fluorophenylalanine, by its action on the mitosis, favours chromosome losses which lead finally to the production of haploid nuclei.

The function of a toluene-degrading bacterial community in a waste gas trickling filter

1999 ◽  
Vol 39 (7) ◽  
pp. 131-137 ◽  
Author(s):  
Anne R. Pedersen ◽  
Erik Arvin
Keyword(s):  
Growth Rate ◽  
Volume Fraction ◽  
Dry Weight ◽  
Maximum Growth ◽  
Maximum Growth Rate ◽  
Trickling Filter ◽  
Biofilm Growth ◽  
Waste Gas ◽  
Degrading Bacteria ◽  
Food Web Model

The function of a community of toluene-degrading bacteria in a biofilm system was investigated with regard to growth and toluene degradation in order to investigate substrate interactions in the community. This was done by the combination of experimental observations using a specific oligonucleotide 16S ribosomal RNA probe targeting the toluene-degrading species Pseudomonas putida, and by computer simulations (AQUASIM) of the biofilm growth based on a food web model. Biofilms were taken from a lab-scale trickling filter for treatment of toluene-polluted air. The biofilm growth and the activity of P. putida, a representative of the toluene-degrading species in the biofilm which have been described previously were simulated. The simulation indicated that the volume fraction of the toluene degraders in the biofilm decreased from 12% to only 2% (11% of dry weight) during two weeks. In spite of the low fraction in the biofilm, the toluene degraders supported growth of the dominating part of non-toluene-degrading heterotrophs in the biofilm, as toluene was the sole carbon source supplied to the system. The maximum intrinsic growth rate of P. putida in the biofilm was only 20% of the maximum growth rate determined in a batch experiment with suspended P. putida cells.

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