Effect of rhizobacteria strains on the induction of resistance in barley genotypes against Cochliobolus sativus

2020 ◽  
Vol 13 (2) ◽  
pp. 83-92 ◽  
Author(s):  
A. Adam

SummaryEnhancement of the resistance level in plants by rhizobacteria has been proven in several pathosystems. This study investigated the ability of four rhizobacteria strains (Pseudomonas putida BTP1 and Bacillus subtilis Bs2500, Bs2504 and Bs2508) to promote the growth in three barley genotypes and protect them against Cochliobolus sativus. Our results demonstrated that all tested rhizobacteria strains had a protective effect on barley genotypes Arabi Abiad, Banteng and WI2291. However, P. putida BTP1 and B. subtilis Bs2508 strains were the most effective as they reduced disease incidence by 53 and 38% (mean effect), respectively. On the other hand, there were significant differences among the rhizobacteria-treated genotypes on plant growth parameters, such as wet weight, dry weight, plant height and number of leaves. Pseudomonas putida BTP1 strain was the most effective as it significantly increased plant growth by 15-32%. In addition, the susceptible genotypes Arabi Abiad and WI2291 were the most responsive to rhizobacteria. This means that these genotypes have a high potential for increase of their resistance against the pathogen and enhancement of plant growth after the application of rhizobacteria. Consequently, barley seed treatment with the tested rhizobacteria could be considered as an effective biocontrol method against C. sativus.

2021 ◽  
Vol 2 ◽  
pp. 234-239
Author(s):  
Nada Kholifah ◽  
Ardiana Kartika B ◽  
Teguh Pribadi

PGPR (Plant Growth Promoting Rhizobacteria) is a substance that helps plant growth with the help of rhizosphere microorganisms. PGPR propagation can be done with liquid media. This PGPR propagation needs to be done because this substance has many benefits for agricultural cultivation. The application of PGPR to the test plant, namely the pakcoy plant, proved that there was an effect of giving PGPR to the plant. Observations on the test plants were carried out by observing several observation variables such as plant height, root length, number of leaves, wet weight and dry weight. The results of these observations showed that the effect on the test plants was seen in the variables of root length, wet weight, and dry weight. Meanwhile, the variable plant height and number of leaves did not show a visible difference. 


Nabatia ◽  
2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Andy Pradana ◽  
Al Machfudz WDP

This study aims to determine the application of potassium fertilizer and chicken manure to the growth and production of shallots (Allium ascalanicum L.). The study used a randomized block design with 2 factors repeated 3 times. Factor 1: Potassium Fertilizer 40,60,80 kg/ha. Factor 2: Chicken Manure 5,10,15 tons/ha. The results showed that the application of potassium fertilizer and chicken manure did not interact with either the growth phase or the production phase of the plant on all observation variables, while the potassium fertilizer treatment showed significantly different results only on the plant vegetative growth parameters, namely the number of leaves at the age of 7 days after planting, namely ( 8,111 strands), while the vegetative phase had no significant effect on the number of tubers per clump, wet weight of tubers per clump, wet weight of tubers per plot, dry weight of sun-dried tubers per clump, and dry weight of sun-dried tubers per plot. While the treatment of chicken manure had a significant effect on the vegetative growth phase of plants, namely the length of plants at 28 DAP (30.278 cm) and 35 DAP (32.704 cm), on the number of leaves at 7 DAP (8,000 strands). While the generative phase (60 DAP) significantly affected the number of tubers per clump, namely (5,889 grams), wet weight of tubers per clump (38,333 grams), wet weight of tubers per plot (319,000 grams), dry weight of sun-dried tubers per clump. namely (23.074 grams), and the dry weight of sun-dried tubers per plot (191.889 grams).


2020 ◽  
Vol 8 (4) ◽  
pp. 496
Author(s):  
Dilfuza Egamberdieva ◽  
Vyacheslav Shurigin ◽  
Burak Alaylar ◽  
Hua Ma ◽  
Marina E. H. Müller ◽  
...  

The effects of biochar on plant growth vary depending on the applied biochar type, study site environmental conditions, microbial species, and plant–microbial interactions. The objectives of the present study were therefore to assess 1) the response of growth parameters of lupin and root disease incidence to the application of three biochar types in a loamy sandy soil, and 2) the role of endophytic bacteria in biological control of root rot disease incidence in lupin after the amendment of soil with different biochar types. As biochar types we tested (i) hydrochar (HTC) from maize silage, (ii) pyrolysis char from maize (MBC), and (iii) pyrolysis char from wood (WBC) at three different concentrations (1%, 2%, and 3% of char as soil amendments). There were no significant effects in lupin shoot and root growth in soils amended with WBC at any of the concentrations. MBC did not affect plant growth except for root dry weight at 2% MBC. HTC char at 2% concentration, significantly increased the root dry weight of lupin by 54–75%, and shoot dry weight by 21–25%. Lupin plants grown in soil amended with 2% and 3% WBC and MBC chars showed 40–50% and 10–20% disease symptoms, respectively. Plants grown in soil without biochar and with HTC char were healthy, and no disease incidence occurred. Pseudomonas putida L2 and Stenotrophomonas pavanii L8 isolates demonstrated a disease reduction compared to un-inoculated plants under MBC and WBC amended soil that was infested with Fusarium solani.


Genetika ◽  
2016 ◽  
Vol 48 (1) ◽  
pp. 233-248
Author(s):  
R. El-Bakatoushi ◽  
A. Elframawy

Plant growth and the expression of two transporter genes; PoHKT1 and PoVHA transcripts in root and shoot tissues were studied under salt stress of three Portulaca oleracea s.l. taxa. The study showed no significant differences in ratios between root lengths in saline and non-saline treatments of the three taxa, which was correlated with a clear down-regulation of the PoHKT1 transcripts in the root after 150mM NaCl. All measured growth parameters except root length increased in P. oleraceae, decreased in P. granulatostellulata and remain unchanged after 100mM NaCl in P. nitida compared to control under saline conditions. The result was consistent with the type of taxon which had significant effect on the shoot length, number of leaves and dry weight (P< 0.05). All measured growth parameters except root length showed a significant negative correlation with the shoot fold change of PoHKT1 transcripts (r = -0.607, -0.693 and -0.657 respectively). The regulation of PoVHA in root and shoot tissues in the three taxa are significantly different. Under salt stress, both decreased uptake of Na+ into the cytosol by decreasing the expression of PoHKT1 and increased vascular compartmentalization ability of Na+ by inducing the expression of PoVHA seem to work more efficiently in P. oleraceae and P. nitida than in P. granulato-stellulata.


Jurnal MIPA ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 150
Author(s):  
Lidyanti Ollo ◽  
Parluhutan Siahaan ◽  
Beivy Kolondam

Penelitian ini bertujuan yang menguji Perbandingan pertumbuhan vegetatif tanaman cabai merah (capsicum Annuum L.) akibat Pemberian Komposisi PGPR (Plant Growth-Promoting Rhizobacteria) yang perbeda. Pertumbuhan tanaman diukur berdasarkan tinggi tanaman, jumlah daun, volume akar, berat basah dan berat kering tanaman. Hasil penelitian menujukkan bahwa PGPR dapat meningkatkan tinggi tanaman, jumlah daun, volume akar, berat basah dan berat kering tanaman cabe.Tinggi tanaman terbaik setelah diperlakuan selama 28 hari adalah dengan pemberian PGPR disertai dengan pemberian pupuk kandang dengan tinggi (24.44 cm), yang diikuti oleh pemberian PGPR + kompos dengan tinggi (22.04 cm), dan pemberian PGPR + NPK dengan tnggi tanaman 19.68 cm . Volume akar akan lebih tinggi bila diberikan PGPR yang ditambah dengan pupuk kandang , atau kompos maupun NPK, tetapi tdak berbeda hasilnya bila hanya diberikan PGPR saja. Jumlah daun dapat ditingkatkan bila diberikan PGPR yang ditambah dengan pupuk kandang, atau kompos maupun NPK. Pemberian PGPR + NPK tidak memberikan jumlah daun yang berbeda dengan pemberian PGPR saja.Berat basah akan meningkat bila diberikan PGPR saja maupun ditambah dengan pemberian pupuk kandang, atau kompos maupun NPK. PGPR ditambah dengan pupuk kandang maupun kompos memberikan dampak yang sama terhadap berat basah.Berat kering akan meningkat bila diberikan PGPR saja maupun ditambah dengan pemberian pupuk kandang, atau kompos maupun NPK. PGPR ditambah dengan pupuk kandang maupun kompos memberikan dampak yang sama terhadap berat basahThis study aimed to examine the comparison of vegetative growth of red chili (capsicum Annuum L.) due to the administration of different PGPR (Plant Growth-Promoting Rhizobacteria) Composition. Plant growth was measured based on plant height, number of leaves, root volume, wet weight and dry weight of the plant. The results showed that PGPR can increase plant height, number of leaves, root volume, wet weight and dry weight of chilli plants. The best plant height after being treated for 28 days is by giving PGPR accompanied by administering manure with height (24.44 cm), followed by by giving PGPR + compost with a height (22.04 cm), and giving PGPR + NPK with a plant height of 19.68 cm. Root volume will be higher if given PGPR added with manure, or compost or NPK, but the results are not different if only given PGPR. The number of leaves can be increased if given PGPR added with manure, or compost or NPK. Provision of PGPR + NPK does not provide a number of leaves that are different from the administration of PGPR alone. Wet weight increased when given PGPR alone or added with manure, or compost or NPK. PGPR added with manure or compost gives the same effect on wet weight. Dry weight will increase if given PGPR alone or added with manure, or compost or NPK. PGPR coupled with manure or compost has the same effect on wet weight


Author(s):  
Badhon Ahmmed ◽  
Tamzid Bin Shafique ◽  
Shaikh Motasim Billah

For agriculture, the most important is the soil’s function as a base and medium for plant growth. The soil health and soil condition are important for plant growth. And the management of soil organic matter is an important part in case of managing soil health and maintaining soil conditions. The experiment was carried out in the field lab (Net House) of Soil, Water and Environment discipline, Khulna University, Bangladesh. Total 21 plastic pots were used to continue the experiment with 7 treatments. For the experiment, the meat bones were collected and used in the experimental pot along with soil in different doses. This research has revealed that meat bones both powdered and composted, has significantly increased (about 20-25%) the growth parameters of water spinach (Ipomoea aquatica) such as a number of leaves, shoot length, fresh weight, dry weight and moisture content. So, the application of powdered and composted meat bones in soil will contribute in the growth of water spinach (Ipomoea aquatica).


2017 ◽  
Vol 1 (2) ◽  
Author(s):  
Nur Hidayatul Alami

Biofertilizers are commonly defined as microbial inoculants which are capable of mobilizing important nutrient in the soil from non-available to available form for the plants through their biological processes. Hence to increase the productivity of the soil, the use of biofertilizer is necessary. Candida yeast as a phosphate solubilizer and decomposer. Azotobacter bacteria as a nitrogen-fixing bacteria and Bacillus as a phosphate solubilizer. The combination of these microorganisms as biofertilizer needs to be tested its effect against mustard (Brassica juncea L.) plant growth.The results showed that the combination of Bacillus Candida obtained the highest yield among other biofertilizer treatments with a value of 13.6 cm at plant height, 4.96 grams in wet weight and 2.29 grams in dry weight. The combination of Azotobacter Candida showed the highest result on the parameter of width and number of leaves with the values of 20.3 cm and 5.3. Combination of Azotobacter Bacillus Candida showed the highest yield on the root length parameter with a value of 5.0 cm. The addition of biofertilizer is expected to reduce pollution on the soil due to residues produced by chemical fertilizers.Keyword : Azotobacter, Bacillus, Biofertilizer, Candida, Mustard Plant


Author(s):  
Mahmoud Ahmed Touny El-Dabaa ◽  
Hassan Abd-El-Khair

Abstract Background Orobanche crenata is an obligate root parasite belonging to Orbanchaceae. Broomrape causes great damage to the faba bean. Several attempts were applied for controlling parasitic weeds. So, the aim of this work is to study the application of Trichoderma spp. as well as three rhizobacteria species in comparison to herbicidal effect of Glyphosate (Glialka 48% WSC) for controlling broomrape infesting faba bean (Vicia faba). Materials and methods Three pot experiments were carried out in the greenhouse of the National Research Centre, Dokki, Giza, Egypt during two successive winter seasons. Trichoderma inocula were adjusted to 3.6 × 108 propagules/ml and the bacterium inocula were adjusted at 107–109 colony-forming unit (CFU)/ml. All treatments were applied, before 1 week of sowing, at rate of 50 ml per pot in experiments I and II, while 100 ml per pot in experiment III. Results Trichoderma spp. (T. harzianum, T. viride and T. vierns) as well as three rhizobacteria species (Pseudomonas fluorescens, Bacillus subtilis and Bacillus pumilus) enhanced the growth parameters in faba bean plants, i.e. shoot length, shoot fresh weight, shoot dry weight and leaf number in the first experiment when applied without O. crenata infection. In the second experiment, all bio-control could protect plants against O. crenata infection, where it had better juvenile number reduction, than glyphosate after 2 months of application. Both B. subtilis and B. pumilus had the highest reduction to juvenile fresh weight, while their effect was equal to herbicide for juvenile dry weight, respectively. The bio-control agents had high effects until the 4th month, but it was less than that of the herbicide. In experiment III, the bio-control agents could highly reduce the juvenile parameters after 2 months, as well as juvenile fresh weight and juvenile dry weight after 4 months, than the herbicide, respectively. The bio-control agents were effective until 6 months, but less than the herbicide effect. All bio-control treatments highly increased the plant growth parameters, than the herbicide. Conclusion The application of Trichoderma spp. as well as rhizobacteria species could play an important role in controlling broomrape in faba bean as a natural bioherbicide.


Author(s):  
Y. Rajasekhara Reddy ◽  
G. Ramanandam ◽  
P. Subbaramamma ◽  
A. V. D. Dorajeerao

A field experiment was carried out during rabi season of 2018-2019, at college farm, College of Horticulture, Dr. Y.S.R. Horticultural University, Venkataramannagudem, West Godavari District, Andhra Pradesh. The experiment was laidout in a Randomised Block Design with eleven treatments (viz., T1- NAA @ 50 ppm, T2-NAA @ 100 ppm, T3-GA3 @ 50 ppm,  T4-GA3 @ 100 ppm, T5-Thiourea @ 250 ppm, T6-Thiourea @ 500 ppm, T7-28-Homobrassinolide @ 0.1 ppm, T8-28-Homobrassinolide @ 0.2 ppm, T9-Triacontinol @ 2.5 ppm, T10-Triacontinol @ 5 ppm, T11-(Control) Water spray) and three replications. The treatments were imposed at 30 and 45 DAT in the form of foliar spray. Foliar application of GA3@ 100 ppm (T4) had recorded the maximum plant height (108.20 cm), leaf area (9.53 cm2) and leaf area index (0.74). Foliar application of thiourea @ 250 ppm (T5) had recorded the maximum values with respect to number of primary branches (15.03 plant-1), number of secondary branches (83.40 plant-1), plant spread (1793 cm2 plant-1), fresh weight (376.29 g plant-1), dry weight (103.54 g plant-1) and number of leaves plant-1((298.8). The same treatment (T5) had recorded the highest values with respect to crop growth rate (1.44 gm-2d-1), chlorophyll-a (1.40 mg g-1), chlorophyll-b (0.076 mg g-1) and total chlorophyll contents (1.48 mg g-1) in the leaves.


2021 ◽  
Vol 940 (1) ◽  
pp. 012026
Author(s):  
D Sumardi ◽  
M Bahariawan ◽  
R R Maulani ◽  
S Suhandono ◽  
C Novia ◽  
...  

Abstract Rice plants (Oryza sativa) are a significant food crop. Tobacco plants (Nicotiana tabacum) can be beneficial and affect growth due to allelochemical content. The srudy aims to determine the effect of tobacco extract (N. tabacum) on the growth and yield of rice (O. sativa) Inpari-32 variety. The study method used the RBD as an experimental design. Three treatments with different concentrations of tobacco extract and one control with three replications. Growth variables were observed: growth rate, plant height, number of tillers, number of leaves, shoot and root dry weight, flowering age, and phytohormones. Observations of the harvest stage include the weight of stover, number of panicle seeds, the total number of tillers, weight of 1000 seeds, the ratio of the weight of filled grain, and productivity (tons/ha). The results showed that although not significant, tobacco extract treatment increased the number of tillers and leaves, the content of phytohormon IAA in the vegetative phase, the content of GA3 in the generative phase, shoot-root ratio, number of tillers, and accelerated flowering age. The implication of tobacco extract positively affects the plant growth variable and accelerated flowering age of rice variety (O. sativa) Inpari 32.


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