scholarly journals Transcriptional analysis of bglPH expression in Bacillus subtilis: evidence for two distinct pathways mediating carbon catabolite repression.

1996 ◽  
Vol 178 (9) ◽  
pp. 2637-2644 ◽  
Author(s):  
S Krüger ◽  
S Gertz ◽  
M Hecker
Keyword(s):  
Bacillus Subtilis ◽  
Catabolite Repression ◽  
Carbon Catabolite Repression ◽  
Transcriptional Analysis

Phosphorylation of HPr and Crh by HprK, Early Steps in the Catabolite Repression Signalling Pathway for the Bacillus subtilis Levanase Operon

1999 ◽  
Vol 181 (9) ◽  
pp. 2966-2969 ◽  
Author(s):  
Isabelle Martin-Verstraete ◽  
Josef Deutscher ◽  
Anne Galinier
Keyword(s):  
Bacillus Subtilis ◽  
Catabolite Repression ◽  
Carbon Catabolite Repression ◽  
Signalling Pathway ◽  
Catabolic Genes

ABSTRACT Carbon catabolite repression (CCR) of Bacillus subtiliscatabolic genes is mediated by CcpA and in part by P-Ser–HPr. For certain operons, Crh, an HPr-like protein, is also implicated in CCR. In this study we demonstrated that in ptsH1 crh1and hprK mutants, expression of the lev operon was completely relieved from CCR and that both P-Ser–HPr and P-Ser–Crh stimulated the binding of CcpA to the cresequence of the lev operon.

Analysis of mutations in the creA gene involved in carbon catabolite repression in Aspergillus nidulans

1996 ◽  
Vol 42 (9) ◽  
pp. 950-959 ◽  
Author(s):  
Robert A. Shroff ◽  
Robin A. Lockington ◽  
Joan M. Kelly
Keyword(s):  
Zinc Finger ◽  
Catabolite Repression ◽  
Carbon Catabolite Repression ◽  
Zinc Finger Protein ◽  
The Other ◽  
Transcriptional Analysis ◽  
Missense Mutations ◽  
Rapid Amplification ◽  
Translational Start ◽  
Carboxy Terminal

The molecular nature of a number of creA mutant alleles has been determined. Three alleles analysed are missense mutations in the DNA binding domain and predicted to reduce but not abolish binding. Of the other four alleles, two result from frameshifts: one has a nonsense mutation and the other has an inversion. All four alleles result in truncations of the protein after the zinc finger domain, such that the protein no longer contains at least the carboxy terminal 145 amino acids, so identifying a region required for repression. Transcriptional analysis of creA indicates that the transcript is autoregulated and analysis using 5′ rapid amplification of cDNA ends indicates that transcriptional start points exist in clusters over a region of 200 bp located up to 595 bp 5′ of the translational start point. The two major clusters have potential CREA-binding sites (SYGGRG) at appropriate positions to allow autoregulation. Autoregulation leads to the creA transcript being most abundant in carbon catabolite nonrepressing conditions, and this, together with the phenotypes of the mutant alleles, has led to the suggestion that CREA has effects under conditions generally not considered as carbon catabolite repressing, as well as in carbon catabolite repressing conditions.Key words: carbon catabolite repression, MIG1, CREA, zinc finger protein, transcriptional repressor.

scholarly journals Drastic Differences in Crh and HPr Synthesis Levels Reflect Their Different Impacts on Catabolite Repression in Bacillus subtilis

2004 ◽  
Vol 186 (10) ◽  
pp. 2992-2995 ◽  
Author(s):  
Boris Görke ◽  
Laetitia Fraysse ◽  
Anne Galinier
Keyword(s):  
Bacillus Subtilis ◽  
Catabolite Repression ◽  
Carbon Catabolite Repression ◽  
Catabolic Genes

ABSTRACT In Bacillus subtilis, carbon catabolite repression (CCR) of catabolic genes is mediated by ATP-dependent phosphorylation of HPr and Crh. Here we show that the different efficiencies with which these two proteins contribute to CCR may be due to the drastic differences in their synthesis rates under conditions that cause CCR.

scholarly journals trans-Acting Factors and cis Elements Involved in Glucose Repression of Arabinan Degradation in Bacillus subtilis

2007 ◽  
Vol 189 (22) ◽  
pp. 8371-8376 ◽  
Author(s):  
José Manuel Inácio ◽  
Isabel de Sá-Nogueira
Keyword(s):  
Bacillus Subtilis ◽  
Growth Phase ◽  
Exponential Growth ◽  
Catabolite Repression ◽  
Carbon Catabolite Repression ◽  
Glucose Repression ◽  
Cis Elements ◽  
Promoter Regions ◽  
Transitional Phase

ABSTRACTInBacillus subtilis, the synthesis of enzymes involved in the degradation of arabinose-containing polysaccharides is subject to carbon catabolite repression (CCR). Here we show that CcpA is the major regulator of repression of the arabinases genes in the presence of glucose. CcpA acts via binding to onecreeach in the promoter regions of theabnAandxsagenes and to twocres in thearaABDLMNPQ-abfAoperon. The contributions of the coeffectors HPr and Crh to CCR differ according to growth phase. HPr dependency occurs during both exponential growth and the transitional phase, while Crh dependency is detected mainly at the transitional phase. Our results suggest that Crh synthesis may increase at the end of exponential growth and consequently contribute to this effect, together with other factors.

scholarly journals Bacillus subtilis Mutant LicT Antiterminators Exhibiting Enzyme I- and HPr-Independent Antitermination Affect Catabolite Repression of the bglPH Operon

2002 ◽  
Vol 184 (17) ◽  
pp. 4819-4828 ◽  
Author(s):  
Cordula Lindner ◽  
Michael Hecker ◽  
Dominique Le Coq ◽  
Josef Deutscher
Keyword(s):  
Amino Acids ◽  
Bacillus Subtilis ◽  
Catabolite Repression ◽  
Carbon Catabolite Repression ◽  
Phosphotransferase System ◽  
Terminal Domain ◽  
Target Sites ◽  
Enzyme I ◽  
In Cells

ABSTRACT The Bacillus subtilis antiterminator LicT regulates the expression of bglPH and bglS, which encode the enzymes for the metabolism of aryl-β-glucosides and the β-glucanase BglS. The N-terminal domain of LicT (first 55 amino acids) prevents the formation of ρ-independent terminators on the respective transcripts by binding to target sites overlapping these terminators. Proteins of the phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS) regulate the antitermination activity of LicT by phosphorylating histidines in its two PTS regulation domains (PRDs). Phosphorylation at His-100 in PRD-1 requires the PTS proteins enzyme I and HPr and the phosphorylated permease BglP and inactivates LicT. During transport and phosphorylation of aryl-β-glucosides, BglP is dephosphorylated, which renders LicT active and thus leads to bglPH and bglS induction. In contrast, phosphorylation at His-207 and/or His-269 in PRD-2, which requires only enzyme I and HPr, is absolutely necessary for LicT activity and bglPH and bglS expression. We isolated spontaneous licT mutants expressing bglPH even when enzyme I and HPr were absent (as indicated by the designation “Pia” [PTS-independent antitermination]). Introduced in a ptsHI + strain, two classes of licT(Pia) mutations could be distinguished. Mutants synthesizing LicT(Pia) antiterminators altered in PRD-2 still required induction by aryl-β-glucosides, whereas mutations affecting PRD-1 caused constitutive bglPH expression. One of the two carbon catabolite repression (CCR) mechanisms operative for bglPH requires the ρ-independent terminator and is probably prevented when LicT is activated by P∼His-HPr-dependent phosphorylation in PRD-2 (where the prefix “P∼” stands for “phospho”). During CCR, the small amount of P∼His-HPr present in cells growing on repressing PTS sugars probably leads to insufficient phosphorylation at PRD-2 of LicT and therefore to reduced bglPH expression. In agreement with this concept, mutants synthesizing a P∼His-HPr-independent LicT(Pia) had lost LicT-modulated CCR.

scholarly journals The Bacillus subtilis crh gene encodes a HPr-like protein involved in carbon catabolite repression

1997 ◽  
Vol 94 (16) ◽  
pp. 8439-8444 ◽  
Author(s):  
A. Galinier ◽  
J. Haiech ◽  
M.-C. Kilhoffer ◽  
M. Jaquinod ◽  
J. Stulke ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Catabolite Repression ◽  
Carbon Catabolite Repression

scholarly journals Expression of the Bacillus subtilis acsAGene: Position and Sequence Context Affect cre-Mediated Carbon Catabolite Repression

1998 ◽  
Vol 180 (24) ◽  
pp. 6649-6654 ◽  
Author(s):  
Jill M. Zalieckas ◽  
Lewis V. Wray ◽  
Susan H. Fisher
Keyword(s):  
Bacillus Subtilis ◽  
Catabolite Repression ◽  
Carbon Catabolite Repression ◽  
Transcriptional Start Site ◽  
Consensus Sequence ◽  
Coupling Factor ◽  
Start Site ◽  
Repression Complex ◽  
Catabolite Repressor ◽  
Transcriptional Start Sites

ABSTRACT In Bacillus subtilis, carbon catabolite repression (CCR) of many genes is mediated at cis-acting carbon repression elements (cre) by the catabolite repressor protein CcpA. Mutations in transcription-repair coupling factor (mfd) partially relieve CCR at cre sites located downstream of transcriptional start sites by abolishing the Mfd-mediated displacement of RNA polymerase stalled at cresites which act as transcriptional roadblocks. Although the acsA cre is centered 44.5 bp downstream of the acsAtranscriptional start site, CCR of acsA expression is not affected by an mfd mutation. When the acsA creis centered 161.5 bp downstream of the transcriptional start site for the unregulated tms promoter, CCR is partially relieved by the mfd mutation. Since CCR mediated at an acsA cre centered 44.5 bp downstream of the tms start site is not affected by the mfd mutation, the inability of Mfd to modulate CCR of acsA expression most likely results from the location of the acsA cre. Higher levels of CCR were found to occur at cre sites flanked by A+T-rich sequences than at cre sites bordered by G and C nucleotides. This suggests that nucleotides adjacent to the proposed 14-bpcre consensus sequence participate in the formation of the CcpA catabolite repression complex at cre sites. Examination of CCR of acsA expression revealed that this regulation required the Crh and seryl-phosphorylated form of the HPr proteins but not glucose kinase.

scholarly journals The Absence of Pyruvate Kinase Affects Glucose-Dependent Carbon Catabolite Repression in Bacillus subtilis

Metabolites ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 216 ◽  
Author(s):  
Sousa ◽  
Westhoff ◽  
Methling ◽  
Lalk
Keyword(s):  
Bacillus Subtilis ◽  
Pyruvate Kinase ◽  
Catabolite Repression ◽  
Carbon Catabolite Repression ◽  
Carbon Sources ◽  
Defined Medium ◽  
Central Carbon Metabolism ◽  
Alternative Pathways ◽  
Distribution Point ◽  
Nutritional Compounds

Pyruvate is a key intermediate of diverse metabolic pathways of central carbon metabolism. In addition to being the end product of glycolysis, pyruvate is an essential carbon distribution point to oxidative metabolism, amino acid and fatty acid syntheses, and overflow metabolite production. Hence, a tight regulation of pyruvate kinase (Pyk) activity is of great importance. This study aimed to analyze targeted metabolites from several pathways and possible changes in Bacillus subtilis lacking Pyk. Wild type and Δpyk cells were cultivated in chemically defined medium with glucose and pyruvate as carbon sources, and the extracted metabolites were analyzed by 1H-NMR, GC-MS, HPLC-MS, and LC-MS/MS. The results showed that the perturbation created in the pyruvate node drove an adaptation to new conditions by altering the nutritional compounds’ consumption. In Δpyk, pyruvate, which is subject to glucose-dependent carbon catabolite repression, did not comply with the hierarchy in carbon source utilization. Other metabolic alterations were observed such as the higher secretion of the overflow metabolites acetoin and 2,3-butanediol by Δpyk. Our results help to elucidate the regulatory transport of glucose and pyruvate in B. subtilis and possible metabolic reroute to alternative pathways in the absence of Pyk.

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