A Note on Detergent Interference in the Serial Dilution Assay of Penicillin Using Bacillus subtilis

The need for a multiple-target compound selectivity programme led to the establishment of a single robotic system that produces a compound's serial dilution and its distribution to multiple replicate assay plates. A Genesis RSP 150 integrated into a Zymate Laboratory Automation System XP produced the serial dilutions, and the subsequent replicate assay plates were produced quickly and accurately by an efficient use of the carousels and rapid plate. Currently, this process allows for the production of over 200 serial dilution assay plates in a workday.

Download Full-text

Statistical Inference for Serial Dilution Assay Data

Biometrics ◽

10.1111/j.0006-341x.1999.01215.x ◽

1999 ◽

Vol 55 (4) ◽

pp. 1215-1220 ◽

Cited By ~ 13

Author(s):

Mei-Ling Ting Lee ◽

G. A. Whitmore

Keyword(s):

Statistical Inference ◽

Serial Dilution ◽

Dilution Assay ◽

Serial Dilution Assay

Download Full-text

Optimization of dye solutions for detecting damaged pancreatic tissues during islet isolation procedures

PLoS ONE ◽

10.1371/journal.pone.0255733 ◽

2021 ◽

Vol 16 (8) ◽

pp. e0255733

Author(s):

Takehiro Imura ◽

Akiko Inagaki ◽

Yasuhiro Igarashi ◽

Masafumi Goto

Keyword(s):

Enzyme Activity ◽

Indigo Carmine ◽

Isolation Procedure ◽

Serial Dilution ◽

Control Group ◽

Islet Isolation ◽

Dna Assays ◽

Serial Dilution Assay ◽

Dye Solutions

We previously reported that dye was effective to prevent the leakage of enzyme solutions from pancreatic glands during an islet isolation procedure. However, the dye used for islet isolation has not yet been optimized. In this study, we focused on pyoktanin blue (PB), diagnogreen (DG), and indigo carmine (IC) as potential candidates among clinically established dyes. A serial dilution assay was performed to determine minimal effective concentrations of each dye for detecting damaged pancreatic tissues. According to the outcome of serial dilution assays, double minimum effective concentrations of each dye were used for in vitro toxicity assays on islets and used in the isolation procedure to investigate whether they adversely affect islet isolation efficiency. The evaluations included islet yield, ADP/ATP, ATP/DNA, glucose stimulation test, and insulin/DNA assays. Islet viability cultured with PB contained medium was significantly lower than the other dyes. DG and IC appeared to be non-toxic to the islets. In isolation experiments, the islet yield in the DG group was considerably lower than that in the Control group, suggesting that DG might inhibit enzyme activity. The present study demonstrates that IC could be a promising candidate for an effective dye to detect damaged pancreatic tissues without affecting the enzyme activity and islet quality.

Download Full-text

Use of Antibody to aid Visualization of Protein at the Termini of Bacteriophage Ø29 DNA

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s1431927600002385 ◽

1980 ◽

Vol 38 ◽

pp. 540-541

Author(s):

Dwight Anderson ◽

Charlene Peterson ◽

Gursaran Notani ◽

Bernard Reilly

Keyword(s):

Electron Microscopy ◽

Bacillus Subtilis ◽

Dna Synthesis ◽

Restriction Endonuclease ◽

Protein Complex ◽

Protein Product ◽

Viral Dna ◽

Small Proteins ◽

Antibody Labeling ◽

Subtilis Bacteriophage

The protein product of cistron 3 of Bacillus subtilis bacteriophage Ø29 is essential for viral DNA synthesis and is covalently bound to the 5’-termini of the Ø29 DNA. When the DNA-protein complex is cleaved with a restriction endonuclease, the protein is bound to the two terminal fragments. The 28,000 dalton protein can be visualized by electron microscopy as a small dot and often is seen only when two ends are in apposition as in multimers or in glutaraldehyde-fixed aggregates. We sought to improve the visibility of these small proteins by use of antibody labeling.

Download Full-text

Antibacterial activity of Celastrol against Bacillus subtilis

Planta Medica ◽

10.1055/s-0028-1084218 ◽

2008 ◽

Vol 74 (09) ◽

Author(s):

N Padilla-Montaño ◽

IL Bazzocchi ◽

L Moujir

Keyword(s):

Antibacterial Activity ◽

Bacillus Subtilis

Download Full-text

Mikrobizide und viruzide Aufbereitung von Dialysegeräten

Dialyse aktuell ◽

10.1055/s-0044-102188 ◽

2018 ◽

Vol 22 (02) ◽

pp. 82-89

Author(s):

Friedrich von Rheinbaben ◽

Oliver Riebe ◽

Johanna Köhnlein ◽

Sebastian Werner

Keyword(s):

Pseudomonas Aeruginosa ◽

Bacillus Subtilis ◽

Phase 3 ◽

Aspergillus Brasiliensis

ZusammenfassungZentrales Bauteil des Genius® 90 Therapie Systems ist der sogenannte Genius-Tank, dem die frische Dialyseflüssigkeit entnommen und in den die verbrauchte Lösung nach der Dialyse zurückgeführt wird. Daher kommt der sicheren Aufbereitung des Systems eine besondere Bedeutung zu. Hierfür wird ein Aufbereitungsverfahren unter Verwendung von UV-Licht in Kombination mit einem chemischen Desinfektionsmittel angewendet. Ziel der hier beschriebenen Untersuchung war es, die Wirkungsbreite und Wirkungstiefe dieses Aufbereitungsverfahrens unter praxisnahen Phase-3-Bedingungen zu ermitteln. Dazu wurde das Gerät mit Mikroorganismen und Viren künstlich kontaminiert und die Wirkung der einzelnen Verfahrensschritte ermittelt. Im Gegensatz zu der üblichen Vorgehensweise praxisnaher Untersuchungen machen Aufbereitungsverfahren medizinischer Geräte unter Phase-3-Kriterien meist eine neuartige Arbeitsweise erforderlich – im Falle der hier vorgestellten Untersuchung sogar die Konstruktion eines speziellen Geräts zur Platzierung von Keimträgen im Genius-Tank. Im Ergebnis konnte gezeigt werden, dass bereits UV-Licht allein sowie in Kombination mit einem chemischen Desinfektionsmittel unter praxisnahen Bedingungen eine sichere Wirksamkeit gegen Bakterien (Pseudomonas aeruginosa) und bakterielle Sporen (Bacillus subtilis), Schimmelpilze (Aspergillus brasiliensis) und Viren (Murines Parvovirus) besitzt.

Download Full-text

Mode of action of 6-oxophenolic triterpenoids against Bacillus subtilis

Planta Medica ◽

10.1055/s-2007-986906 ◽

2007 ◽

Vol 73 (09) ◽

Author(s):

L Moujir ◽

L de León ◽

IL Bazzocchi

Keyword(s):

Bacillus Subtilis ◽

Mode Of Action

Download Full-text

Aplikasi Bacillus subtilis pada beberapa Bahan Organik terhadap Pertumbuhan dan Produksi Tanaman Cabai Rawit (Capsicum frutescens L.)

JURNAL AGRI-TEK Jurnal Penelitian Ilmu-Ilmu Eksakta ◽

10.33319/agtek.v21i1.69 ◽

2020 ◽

Vol 21 (1) ◽

pp. 14-19

Author(s):

Praptiningsih Gamawati Adinurani ◽

Sri Rahayu ◽

Nurul Fima Zahroh

Keyword(s):

Bacillus Subtilis ◽

Plant Growth ◽

Plant Growth Promoting Rhizobacteria ◽

Capsicum Frutescens ◽

Plant Growth Promoting ◽

Growth Promoting

Mikroba Bacillus subtilis merupakan agen pengendali hayati mempunyai kelebihan sebagai Plant Growth Promoting Rhizobacteria (PGPR) yaitu dapat berfungsi sebagai biofertilizer, biostimulan, biodekomposer dan bioprotektan. Tujuan penelitian mengetahui potensi B. subtilis dalam merombak bahan organik sebagai usaha meningkatkan ketersediaan bahan organik tanah yang semakin menurun. Penelitian menggunakan Rancangan Petak Terbagi dengan berbagai bahan organik sebagai petak utama (B0 = tanpa bahan organik, B1 = kotoran ayam, B2 = kotoran kambing, B3 = kotoran sapi) dan aplikasi B.subtilis sebagai anak petak (A0 = 0 cc/L, A1 = 5cc/L, A2 = 10 cc/L, Pengamatan meliputi variabel tinggi tanaman, indeks luas daun, jumlah buah per tanaman, berat buah per tanaman, dan bahan organik tanah. Data pengamatan dianalisis ragam menggunakan Statistical Product and Service Solutions (SPSS) versi 25 dan dilanjutkan dengan uji Duncan untuk mengetahui signifikansi perbedaan antar perlakuan. Hasil penelitian menunjukkan tidak terdapat interaksi antara bahan organik kotoran ternak dan konsentrasi B. subtilis terhadap semua variabel pengamatan. Potensi B. subtilis sangat baik dalam mendekomposisi bahan organik yang ditunjukkan dengan peningkatan bahan organik, dan hasil terbaik pada kotoran sapi (B3) dan konsentrasi B. subtilis 15 mL/L masing-masing sebesar 46.47 % dan 34.76 %. Variabel pertumbuhan tidak berbeda nyata kecuali tinggi tanaman dengan pertambahan tinggi paling banyak pada pemberian kotoran kambing sebesar 170.69 %.

Download Full-text