Serial Dilution Assay

2005 ◽  
Author(s):  
Martin Ridout
Keyword(s):  
Serial Dilution ◽  
Dilution Assay ◽  
Serial Dilution Assay

scholarly journals Optimizing production of serially diluted compounds and distribution to multiple targets

2001 ◽  
Vol 23 (6) ◽  
pp. 179-182 ◽  
Author(s):  
Cole O. Harris ◽  
Stephanie L. Schweiker
Keyword(s):  
Robotic System ◽  
Serial Dilution ◽  
Automation System ◽  
Laboratory Automation ◽  
Multiple Target ◽  
Target Compound ◽  
Multiple Targets ◽  
Dilution Assay ◽  
Serial Dilution Assay ◽  
Serial Dilutions

The need for a multiple-target compound selectivity programme led to the establishment of a single robotic system that produces a compound's serial dilution and its distribution to multiple replicate assay plates. A Genesis RSP 150 integrated into a Zymate Laboratory Automation System XP produced the serial dilutions, and the subsequent replicate assay plates were produced quickly and accurately by an efficient use of the carousels and rapid plate. Currently, this process allows for the production of over 200 serial dilution assay plates in a workday.

Statistical Inference for Serial Dilution Assay Data

Biometrics ◽  
1999 ◽  
Vol 55 (4) ◽  
pp. 1215-1220 ◽  
Author(s):  
Mei-Ling Ting Lee ◽  
G. A. Whitmore
Keyword(s):  
Statistical Inference ◽  
Serial Dilution ◽  
Dilution Assay ◽  
Serial Dilution Assay

scholarly journals Optimization of dye solutions for detecting damaged pancreatic tissues during islet isolation procedures

PLoS ONE ◽  
2021 ◽  
Vol 16 (8) ◽  
pp. e0255733
Author(s):  
Takehiro Imura ◽  
Akiko Inagaki ◽  
Yasuhiro Igarashi ◽  
Masafumi Goto
Keyword(s):  
Enzyme Activity ◽  
Indigo Carmine ◽  
Isolation Procedure ◽  
Serial Dilution ◽  
Control Group ◽  
Islet Isolation ◽  
Dna Assays ◽  
Serial Dilution Assay ◽  
Dye Solutions

We previously reported that dye was effective to prevent the leakage of enzyme solutions from pancreatic glands during an islet isolation procedure. However, the dye used for islet isolation has not yet been optimized. In this study, we focused on pyoktanin blue (PB), diagnogreen (DG), and indigo carmine (IC) as potential candidates among clinically established dyes. A serial dilution assay was performed to determine minimal effective concentrations of each dye for detecting damaged pancreatic tissues. According to the outcome of serial dilution assays, double minimum effective concentrations of each dye were used for in vitro toxicity assays on islets and used in the isolation procedure to investigate whether they adversely affect islet isolation efficiency. The evaluations included islet yield, ADP/ATP, ATP/DNA, glucose stimulation test, and insulin/DNA assays. Islet viability cultured with PB contained medium was significantly lower than the other dyes. DG and IC appeared to be non-toxic to the islets. In isolation experiments, the islet yield in the DG group was considerably lower than that in the Control group, suggesting that DG might inhibit enzyme activity. The present study demonstrates that IC could be a promising candidate for an effective dye to detect damaged pancreatic tissues without affecting the enzyme activity and islet quality.

scholarly journals STUDIES ON THE BACTERIOPHAGE OF D'HERELLE

1925 ◽  
Vol 42 (4) ◽  
pp. 483-497 ◽  
Author(s):  
Jacques J. Bronfenbrenner ◽  
Charles Korb
Keyword(s):  
Quantitative Evaluation ◽  
Bacterial Species ◽  
Relative Concentration ◽  
Serial Dilution ◽  
The Other ◽  
Resistant Bacteria ◽  
Dilution Method ◽  
High Concentration ◽  
Average Size ◽  
Broth Dilution

The experiments reported above confirm the fact that lytic principle is distributed in active solution in a state of indivisible units. This permits its quantitative evaluation by serial dilution, as well as by plating on agar. The latter method, however, often gives readings considerably lower than those obtained by the broth dilution method of titration. By varying the concentration of agar it has been possible to show that the discrepancy is due to adsorption of the lytic agent on agar. When the concentration of the latter is increased from 0.3 per cent to 2.5 per cent the number of plaques of lysis is reduced more than 100 times. At the same time the average size of the plaques also decreases approximately to one-tenth of the original. The size, as well as the number of plaques, has been found to depend also on the condition of the culture employed in titration. Thus, when the culture exposed to the action of lytic agent is composed of young susceptible bacteria, the greater the concentration of bacteria, the smaller the plaques. When the culture is composed partly of young and partly of old susceptible bacteria, both the size and the number of the plaques are diminished with the increase in the relative concentration of old bacteria. On the other hand, presence in the culture of resistant bacteria does not affect either the size or the number of the plaques so long as the relative concentration of susceptible bacteria in the culture is sufficient to allow formation of them. The plaques appearing in the presence of a high concentration of resistant variants in the culture are relatively indistinct owing to overgrowth. Under carefully controlled conditions the size of plaques is found to be determined by the character of the lytic filtrate. Thus in the case of lytic agents which act upon more than one bacterial species the size of the plaques remains constant, irrespective of the bacterial substratum used for the production of the active filtrate.

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