scholarly journals Multicenter Clinical Evaluation of BacT/Alert Virtuo Blood Culture System

2017 ◽  
Vol 55 (8) ◽  
pp. 2413-2421 ◽  
Author(s):  
Michael R. Jacobs ◽  
Tony Mazzulli ◽  
Kevin C. Hazen ◽  
Caryn E. Good ◽  
Ayman M. Abdelhamed ◽  
...  
Keyword(s):  
Blood Culture ◽  
Culture System ◽  
Microbial Growth ◽  
Fungal Growth ◽  
Detection Algorithm ◽  
Blood Culture System ◽  
Gram Negative ◽  
Time To Detection ◽  
Clinically Significant ◽  
Organism Group

ABSTRACTBacT/Alert Virtuo is an advanced, automated blood culture system incorporating improved automation and an enhanced detection algorithm to shorten time to detection. A multicenter study of the investigational Virtuo system (bioMérieux, Inc., Durham, NC) compared to BacT/Alert 3D (BTA3D) for detection of bacteremia/fungemia in four bottle types, SA and FA Plus (aerobic) and SN and FN Plus (anaerobic), was performed in a clinical setting with patient samples in a matched system design clinical trial. Blood was added to paired aerobic or anaerobic bottles, with the volume in each bottle in each pair required to be ≤10 ml and with the volumes required to be within 30% of each other. Of 5,709 bottle sets (52.5% aerobic pairs and 47.5% anaerobic pairs), 430 (7.5%) were positive for bacterial or fungal growth, with 342 (6.0%) clinically significant and 83 (1.5%) contaminated. A total of 3,539 sets (62.0%) were volume compliant, with 203 sets (5.7%) clinically significant. The positivity rates for volume-compliant bottle pairs determined by the two systems were comparable, with 68.7% of clinically significant isolates detected by both instruments, 15.7% by Virtuo only, and 15.7% by BTA3D only. Virtuo detected microbial growth nearly 2 h sooner overall than BTA3D (mean, 15.9 h versus 17.7 h). Shorter time to detection by Virtuo was related to organism group, with the time to detection being significantly shorter for enteric Gram-negative bacilli and enterococci (means, 3.6 h and 2.3 h shorter, respectively). This large clinical study demonstrated that the Virtuo blood culture system produced results comparable to those seen with the long-established BTA3D system, with significantly shorter time to detection.

scholarly journals Superior Sensitivity and Decreased Time to Detection with the Bactec Peds Plus/F System Compared to the BacT/Alert Pediatric FAN Blood Culture System

2013 ◽  
Vol 51 (12) ◽  
pp. 4083-4086 ◽  
Author(s):  
K. V. Sullivan ◽  
N. N. Turner ◽  
D. P. Lancaster ◽  
A. R. Shah ◽  
L. J. Chandler ◽  
...  
Keyword(s):  
Blood Culture ◽  
Culture System ◽  
Blood Culture System ◽  
Time To Detection ◽  
F System

scholarly journals Evaluation of blood culture procedures in a pediatric hospital

1979 ◽  
Vol 9 (1) ◽  
pp. 88-92
Author(s):  
E G Szymczak ◽  
J T Barr ◽  
W A Durbin ◽  
D A Goldmann
Keyword(s):  
Blood Culture ◽  
Culture System ◽  
Clinical Laboratory ◽  
Blood Cultures ◽  
Pediatric Hospital ◽  
Visual Examination ◽  
Clinical Laboratory Techniques ◽  
Blood Culture System ◽  
Laboratory Techniques ◽  
Clinically Significant

To determine optimal clinical laboratory techniques for detecting pediatric bacteremia, we studied 7,768 consecutive blood cultures in a 1-year period. Blood was inoculated into one vented 50-ml bottle of brucella broth with 0.05% sodium polyanetholsulfonate and one unvented 50-ml bottle of Columbia broth with 0.05% sodium polyanetholsulfonate and 0.05% cysteine. Bottles were visually examined for growth on days 1 through 7 and blindly subcultured aerobically and anaerobically on days 1, 2, and 7. There were 724 (9.3%) positive cultures, and 484 (6.2%) were clinically significant. The most frequent isolates from bacteremic patients were Haemophilus influenzae (24%) and Streptococcus pneumoniae (17%). Growth was noted in only one bottle in 25% of clinically significant isolates. Bottles inoculated with greater than or equal to 1 ml of blood became positive earlier than bottles inoculated with less than 1 ml. After 1 day of incubation, 48% of the clinically significant cultures showed growth on visual examination, whereas 85% showed growth on subculture. Only 19% of Haemophilus isolates were detected visually on day 1, whereas 88% were recovered on subculture. By day 7, 3.5% of all isolates (including 18% of pneumococcal isolates and 1% of Haemophilus isolates) could no longer be recovered on subculture. We conclude that a two-bottle blood culture system and blind subculture within 24 h will optimize detection of pediatric bacteremia.

scholarly journals Evaluation of BACTEC MYCO/F Lytic Medium for Recovery of Mycobacteria and Fungi from Blood

1998 ◽  
Vol 36 (5) ◽  
pp. 1176-1179 ◽  
Author(s):  
Rebecca T. Waite ◽  
Gail L. Woods
Keyword(s):  
Blood Culture ◽  
Culture System ◽  
Histoplasma Capsulatum ◽  
Mycobacterium Kansasii ◽  
Fungal Isolation ◽  
Blood Culture System ◽  
Valid Assessment ◽  
The Mean ◽  
Time To Detection ◽  
Mean Time

The reliability of MYCO/F Lytic medium in the BACTEC 9240 blood culture system was evaluated by comparing its performance to that of the Isolator system for the recovery of fungi and to that of the ESP II system for the recovery of mycobacteria. Of 717 specimens of blood cultured for fungi, 24 were positive; 12 samples were positive with both systems, 7 samples were positive with the Isolator system only, and 5 samples were positive with MYCO/F Lytic medium only. Fourteen samples grew Histoplasma capsulatum; both systems detectedH. capsulatum in seven samples but the Isolator system alone detected H. capsulatum in seven samples. The mean times to the detection of H. capsulatum were 8 days (range, 4 to 13 days) for MYCO/F Lytic medium and 9 days (range, 6 to 18 days) for the Isolator system; the mean times to identification were 20 days (range, 15 to 24 days) for isolates recovered with MYCO/F Lytic medium and 11 days (range, 6 to 18 days) for those recovered with the Isolator system (P < 0.05). Cryptococcus neoformans was isolated from 10 fungal cultures; five isolates grew in both systems, and five isolates grew in MYCO/F Lytic medium only. The mean times to detection of C. neoformans were 4 days (range, 2 to 6 days) for MYCO/F Lytic medium and 7 days (range, 5 to 7 days) for the Isolator system (P < 0.05); the mean times to identification were 15 days (range, 7 to 27 days) for isolates recovered with MYCO/F Lytic medium and 8 days (range, 7 to 11 days) for those recovered with the Isolator system. Of the 687 samples of blood cultured for mycobacteria, 64 blood samples from 42 patients grew mycobacteria (58 grew Mycobacterium avium complex, 4 grew Mycobacterium kansasii, and 2 grew Mycobacterium tuberculosis); 42 isolates were recovered with both systems, 18 were isolated with MYCO/F medium only, and 4 were isolated with the ESP II system only alone (P < 0.05). The mean time to detection of mycobacteria with MYCO/F Lytic medium was 14 days, whereas it was 17 days with the ESP II system (P < 0.05). In summary, the combination of MYCO/F Lytic medium and the BACTEC 9240 instrument is an excellent blood culture system for the growth and detection of mycobacteria. A valid assessment of MYCO/F Lytic medium with regard to fungal isolation, however, was not possible due to the small number of isolates recovered.

scholarly journals Controlled Evaluation of the New BacT/Alert Virtuo Blood Culture System for Detection and Time to Detection of Bacteria and Yeasts

2016 ◽  
Vol 54 (4) ◽  
pp. 1148-1151 ◽  
Author(s):  
Osman Altun ◽  
Mohammed Almuhayawi ◽  
Petra Lüthje ◽  
Rubina Taha ◽  
Måns Ullberg ◽  
...  
Keyword(s):  
Blood Culture ◽  
Culture System ◽  
Detection Rate ◽  
Blood Culture System ◽  
Fungal Isolates ◽  
Controlled Evaluation ◽  
Time To Detection

We compared the newly approved BacT/Alert Virtuo blood culture system to the BacT/Alert 3D system using 115 clinical bacterial and fungal isolates in 784 simulated blood culture bottles. The time to detection was reduced by roughly 20% in the Virtuo system (P< 0.0001) while the detection rate did not differ.

COMPARATIVE EVALUATION OF BLOOD CULTURE BY CONVENTIONAL METHOD AND BACTEC USING BLOOD SAMPLES OF ICU PATIENTS and ANTIBIOGRAM OF ISOLATED PATHOGENS

2016 ◽  
Vol 1 (2) ◽  
Author(s):  
Esha Singhal ◽  
Rahul Kumar Goyal ◽  
R. N. Behara
Keyword(s):  
Blood Culture ◽  
Conventional Method ◽  
Predictive Value ◽  
Culture System ◽  
Multi Drug Resistance ◽  
Gram Positive ◽  
Icu Patients ◽  
Blood Culture System ◽  
Blood Samples ◽  
Gram Negative

INTRODUCTION: Sepsis is the leading cause of death in Intensive ICU patients worldwide.Blood cultures are the ‘gold standard’ for identifying pathogens causing septicemia and in directing appropriate antibiotic therapy. The present study was aimed to compare sensitivity of automated BACTEC 9120 blood culture system and conventional blood culture method in identifying true pathogenic organism, to compare the time needed for the detection of microorganisms by conventional method of blood culture and by automated BACTEC 9120 blood culture system and to evaluate the susceptibility pattern of antibiotics for pathogens isolated. MATERIAL AND METHODS: Study was done over a period of one year from April 2015 to March 2016. A total of 636 blood samples were collected andsubjected to blood culture by BACTEC 9120 and conventional method. The statistical tests applied were Sensitivity, Specificity, Positive predictive value, Negative predictive value, T-test and kappa statistics. RESULTS: Out of 636 blood samples clinically significant isolates were recovered from 85 samples by BACTEC, of which 74 were bacterial pathogens and 11 were candida. In conventional method out of 80 significant isolates 69 were bacterial and 11 were candida. Gram positive (majority Staphylococci) were more commonly isolated than Gram negative (majority Acinetobacter and E.coli). Mean time to detect was 19.47 hours and 3.02 days, by BACTEC 9120 and conventional method respectively. Vancomycin, Teicoplanin and Linezolid were found to be the most effective (100%) for gram positive bacteria and for gram negative bacteria Colistin (70-100%), Polymyxin-B (70-100%) were the most effective. CONCLUSION: Sepsis is associated with prolonged hospital stay, increased costs and with a high mortality. The use of BACTEC BD blood culture system is better for rapid identification of blood borne pathogens followed by determining actual antimicrobial treatment in the scenario of multi drug resistance so as to improve patient’s outcome.

scholarly journals Study of blood cultures by BACTEC method in pediatric patients of Chhattisgarh, India

2019 ◽  
Vol 6 (5) ◽  
pp. 2022
Author(s):  
Meenakshi S. Morey ◽  
Nirmal Channe
Keyword(s):  
Blood Culture ◽  
Clavulanic Acid ◽  
Culture System ◽  
Microbial Growth ◽  
Pediatric Patients ◽  
Blood Cultures ◽  
Culture Study ◽  
Blood Culture System ◽  
E Coli ◽  
Life Threatening

Background: 356 children aged  between 2 to 4 years admitted at PICU were selected for study B.D BACTEC 9050 system was used for incubation bottle  were incubated until microbial growth was detected, BACTEC 9050 is an automated blood culture system which responds to concentration of  Co2 produced by metabolism microorganism or consumption of O2, needed for growth of microorganism.Methods: The antibiotic used were Amoxicin 250 mcg, penicillin 100 unit linezoid 50 Mcg, vancomycin 50 mcg, ampicillin 100 mcg, Azithromycin 100 Mcg, pipericillin/ Tozabactum, 100/50mcg, ceforazone/ salbactum 50/30 mcg, cefoxitin 50 mcg, cefepime 50 mcg, Amikacin 50 mcg, Imipenen 30 mcg, ceftazidime- clavulanic acid..Results: The observed organized were 90 (25.2%) CoNS, 72 (20.2%) Entrobacter, 70 (19.6%) Klebesiella  spp, 40(11.1%) Enterococcus, 22(6.1%) S, auresis, 22 (6.1%) E coli,  20(5.6%) citrobacters, 20(5.6%) Acinitobacter. This blood culture study  by BACTEC 9050 in pediatric patients in few minutes.Conclusions: BACTEC-9050 method was helpful to treat especially in children because most of diseases in children are idiopathic and life threatening.

scholarly journals Rapid Identification of Burkholderia pseudomallei in Blood Cultures by a Monoclonal Antibody Assay

1999 ◽  
Vol 37 (11) ◽  
pp. 3662-3667 ◽  
Author(s):  
Supinya Pongsunk ◽  
Nittaya Thirawattanasuk ◽  
Nuanchan Piyasangthong ◽  
Pattama Ekpo
Keyword(s):  
Monoclonal Antibody ◽  
Blood Culture ◽  
Culture System ◽  
Burkholderia Pseudomallei ◽  
Bacterial Culture ◽  
Brain Heart Infusion ◽  
Culture Method ◽  
Blood Culture System ◽  
Gram Negative

Burkholderia pseudomallei is the causative agent of melioidosis. In northeast Thailand, this gram-negative bacterium is a major cause of mortality from septicemia. The definitive diagnosis of this disease is made by bacterial culture. In this study, we produced a monoclonal antibody (MAb) specific to the 30-kDa protein of B. pseudomallei by in vivo and in vitro immunization of BALB/c mice with a crude culture filtrate antigen. The MAb could directly agglutinate with all 243 clinical isolates of B. pseudomallei but not with other gram-negative bacteria, except for one strain of Burkholderia mallei. However, the MAb cross-reacted with the gram-positive Bacillus sp. andStreptococcus pyogenes. B. pseudomallei in brain heart infusion broth (BHIB) subcultured from a BacT/Alert automated blood culture system could be identified by simple agglutination with this MAb assay. The sensitivity and specificity of direct agglutination compared to the “gold standard,” the culture method, were 94.12 and 98.25%, respectively. However, the MAb adsorbed to polystyrene beads or latex particles directly identified the bacterium in blood culture specimens and in BHIB subcultured from a BacT/Alert automated blood culture system. The sensitivity of the latex agglutination test was 100% for both blood culture and BHIB specimens. The specificity was 85.96 and 96.49% for the blood culture and BHIB specimens, respectively. The specificity could be increased if the nonspecific materials in the blood culture broths were eradicated by centrifugation at low speeds. Thus, a combination of blood culture and the agglutination method could be used for the rapid diagnosis of melioidosis in the routine bacteriological laboratory. This method could speed up detection of the bacterium in blood culture by at least 2 days, compared to the conventional bacterial culture method. In addition, the MAb is stable at room temperature for 2 weeks and at 4, −20, and −70°C for at least 1 year. The latex reagent was stable for at least 6 months at 4°C.

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