scholarly journals Development and Application of a Novel Peptide Nucleic Acid Probe for the Specific Detection of Helicobacter pylori in Gastric Biopsy Specimens

2007 ◽  
Vol 45 (9) ◽  
pp. 3089-3094 ◽  
Author(s):  
N. Guimaraes ◽  
N. F. Azevedo ◽  
C. Figueiredo ◽  
C. W. Keevil ◽  
M. J. Vieira
Keyword(s):  
Helicobacter Pylori ◽  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Gastric Biopsy ◽  
Specific Detection ◽  
Peptide Nucleic Acid Probe ◽  
Biopsy Specimens

scholarly journals Development and Application of a Novel Peptide Nucleic Acid Probe for the Specific Detection of Cronobacter Genomospecies (Enterobacter sakazakii) in Powdered Infant Formula

2009 ◽  
Vol 75 (9) ◽  
pp. 2925-2930 ◽  
Author(s):  
C. Almeida ◽  
N. F. Azevedo ◽  
C. Iversen ◽  
S. Fanning ◽  
C. W. Keevil ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Infant Formula ◽  
Peptide Nucleic Acid ◽  
Powdered Infant Formula ◽  
Enterobacter Sakazakii ◽  
Specific Detection ◽  
Peptide Nucleic Acid Probe ◽  
Bacterial Contaminants ◽  
Specificity And Sensitivity

ABSTRACT Here, we report a fluorescence in situ hybridization (FISH) method for rapid detection of Cronobacter strains in powdered infant formula (PIF) using a novel peptide nucleic acid (PNA) probe. Laboratory tests with several Enterobacteriaceae species showed that the specificity and sensitivity of the method were 100%. FISH using PNA could detect as few as 1 CFU per 10 g of Cronobacter in PIF after an 8-h enrichment step, even in a mixed population containing bacterial contaminants.

Detection of Helicobacter pylori CagA EPIYA in gastric biopsy specimens and its relation to gastric diseases

2015 ◽  
Vol 83 (2) ◽  
pp. 89-92 ◽  
Author(s):  
Júlia Silveira Vianna ◽  
Ivy Bastos Ramis ◽  
Priscila Cristina Bartolomeu Halicki ◽  
Otávio Leite Gastal ◽  
Renato Azevedo Silva ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Gastric Biopsy ◽  
Gastric Diseases ◽  
Biopsy Specimens

Evaluation of Selective and Nonselective Media for Isolation of Helicobacter pylori from Gastric Biopsy Specimens

2007 ◽  
Vol 10 (22) ◽  
pp. 4156-4159 ◽  
Author(s):  
M.Y. Alikhani ◽  
N. Sadeghifar ◽  
Safar Farajnia . ◽  
M. Hajia ◽  
M.M. Aslani ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Gastric Biopsy ◽  
Biopsy Specimens

scholarly journals Peptide nucleic acid-templated selenocystine–selenoester ligation enables rapid miRNA detection

2018 ◽  
Vol 9 (4) ◽  
pp. 896-903 ◽  
Author(s):  
Jessica Sayers ◽  
Richard J. Payne ◽  
Nicolas Winssinger
Keyword(s):  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Specific Detection ◽  
Ligation Reaction ◽  
Low Concentrations ◽  
Mirna Detection ◽  
Peptide Ligation

A PNA-templated peptide ligation reaction has been developed between selenocystine and selenoesters. The methodology was used for the sequence specific detection of miRNA at low concentrations.

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