scholarly journals Development of Fully Automated Determination of Marker-Specific Immunoglobulin G (IgG) Avidity Based on the Avidity Competition Assay Format: Application for Abbott Architect Cytomegalovirus and Toxo IgG Avidity Assays

2009 ◽  
Vol 47 (3) ◽  
pp. 603-613 ◽  
Author(s):  
I. Curdt ◽  
G. Praast ◽  
E. Sickinger ◽  
J. Schultess ◽  
I. Herold ◽  
...  
Keyword(s):  
Immunoglobulin G ◽  
Competition Assay ◽  
Igg Avidity ◽  
Assay Format ◽  
Specific Immunoglobulin ◽  
Automated Determination ◽  
Abbott Architect

scholarly journals Development of a Rapid and Convenient Method for Determination of Rubella Virus-Specific Immunoglobulin G Avidity

2007 ◽  
Vol 14 (11) ◽  
pp. 1416-1419 ◽  
Author(s):  
Christelle Vauloup-Fellous ◽  
Jessica Ursulet-Diser ◽  
Liliane Grangeot-Keros
Keyword(s):  
Immunoglobulin G ◽  
Rubella Virus ◽  
Primary Infection ◽  
Virus Infections ◽  
Serum Samples ◽  
Igg Avidity ◽  
Specific Immunoglobulin ◽  
Specific Igg ◽  
Semiautomated Method

ABSTRACT We describe here a rapid and semiautomated method for the determination of rubella virus immunoglobulin G (IgG) avidity with the VIDAS instrument. A total of 153 serum samples from persons with naturally acquired rubella virus infections (n = 98), from vaccinated persons (n = 44), and from patients with autoantibodies (n = 11) were included in this study. The rubella virus-specific IgG avidity assay we developed for the VIDAS instrument was evaluated by comparison with an in-house method. Results obtained with the VIDAS instrument allow considering this method valuable to help confirm or exclude acute primary infection or recent vaccination.

scholarly journals Longitudinal monitoring of SARS-CoV-2-specific immune responses

2021 ◽  
Author(s):  
Heike Rebholz ◽  
Ralf J. Braun ◽  
Titas Saha ◽  
Oliver Harzer ◽  
Miriam Schneider ◽  
...  
Keyword(s):  
T Cell ◽  
Immune Responses ◽  
Immunoglobulin G ◽  
Immunoglobulin A ◽  
Comprehensive View ◽  
Iga Antibodies ◽  
Specific Immunoglobulin ◽  
Second Peak

The Lower Austrian Wachau region was an early COVID-19 hotspot of infection. As previously reported, in June 2020, after the first peak of infections, we determined that 8.5% and 9.0% of the participants in Weissenkirchen and surrounding communities in the Wachau region were positive for SARS-CoV-2-specific immunoglobulin G (IgG) and immunoglobulin A (IgA) antibodies, respectively. Here, we present novel data obtained eight months later (February 2021) from Weissenkirchen, after the second peak of infection, with 25.0% (138/552) and 23.6% (130/552) of participants that are positive for IgG and IgA, respectively. In participants with previous IgG/IgA positivity (June 2020), we observed a 24% reduction in IgG levels, whereas the IgA levels remained stable in February 2021. This subgroup was further analyzed for SARS-CoV-2-induced T cell activities. Although 76% (34/45) and 76% (34/45) of IgG positive and IgA positive participants, respectively, showed specific T cell activities, those were not significantly correlated with the levels of IgG or IgA. Thus, the analyses of antibodies cannot surrogate the measurement of T cell activities. For a comprehensive view on SARS-CoV-2-triggered immune responses, the measurement of different classes of antibodies should be complemented with the determination of T cell activities.

Differential pulse voltammetric enzyme-linked immunoassay for the determination of Helicobacter pylori specific immunoglobulin G (IgG) antibody

Talanta ◽  
1997 ◽  
Vol 44 (5) ◽  
pp. 823-830 ◽  
Author(s):  
Ya-nan He ◽  
Hong-yuan Chen ◽  
Jin-juan Zheng ◽  
Guang-yu Zhang ◽  
Zeng-Lan Chen
Keyword(s):  
Helicobacter Pylori ◽  
Immunoglobulin G ◽  
Differential Pulse ◽  
Igg Antibody ◽  
Specific Immunoglobulin

scholarly journals Micro-bead injection spectroscopy for label-free automated determination of immunoglobulin G in human serum

2017 ◽  
Vol 410 (3) ◽  
pp. 981-988 ◽  
Author(s):  
Inês I. Ramos ◽  
Luís M. Magalhães ◽  
Luisa Barreiros ◽  
Salette Reis ◽  
José L. F. C. Lima ◽  
...  
Keyword(s):  
Human Serum ◽  
Immunoglobulin G ◽  
Label Free ◽  
Bead Injection ◽  
Automated Determination

scholarly journals Assignment of Neisseria meningitidis Serogroups A, C, W135, and Y Anticapsular Total Immunoglobulin G (IgG), IgG1, and IgG2 Concentrations to Reference Sera

2004 ◽  
Vol 11 (1) ◽  
pp. 1-5 ◽  
Author(s):  
Helen Joseph ◽  
Paul Balmer ◽  
Mike Bybel ◽  
Thomas Papa ◽  
Robert Ryall ◽  
...  
Keyword(s):  
Single Dose ◽  
Immunoglobulin G ◽  
Enzyme Linked Immunosorbent Assay ◽  
Specific Immunoglobulin ◽  
Detailed Evaluation ◽  
Specific Igg ◽  
Assay Protocol ◽  
Total Immunoglobulin ◽  
Good Agreement

ABSTRACT Meningococcal serogroup-specific immunoglobulin G (IgG), IgG1, and IgG2 concentrations were assigned to three reference sera, CDC 1992, 89-SF, and 96/562, for meningococcal serogroups A, C, Y, and W135 via the method of cross standardization. The sum of the serogroup-specific IgG1 and IgG2 concentrations determined for the four meningococcal serogroups showed good agreement with the serogroup-specific IgG either determined here or as previously represented. Following the assignment of meningococcal serogroup-specific IgG1 and IgG2 concentration to these reference sera, a meningococcal serogroup-specific IgG1 and IgG2 enzyme-linked immunosorbent assay protocol was developed. The serogroup A and C specific subclass distribution of a panel of adult sera collected following vaccination with any combination of meningococcal serogroup C conjugate, bivalent, or tetravalent polysaccharide vaccines was determined. For the determination of serogroup W135 and Y specific subclass distribution, an adolescent panel 28 days following a single dose of either tetravalent polysaccharide or conjugate vaccine was used. The sum of the serogroup-specific IgG1 and IgG2 showed strong correlation with the serogroup-specific total IgG determined. The assignment here of IgG1 and IgG2 subclasses to these reference sera will allow more detailed evaluation of meningococcal conjugate and polysaccharide vaccines.

Use of Protein a Conjugated to Peroxidase to Localize Immunoglobulin G in SSPE Ferret Brain

1982 ◽  
Vol 40 ◽  
pp. 350-351
Author(s):  
Hannah R. Brown ◽  
Anthony F. Nostro ◽  
Halldor Thormar
Keyword(s):  
Immunoglobulin G ◽  
Human Disease ◽  
Measles Virus ◽  
Subacute Sclerosing Panencephalitis ◽  
Protein A ◽  
Inflammatory Lesions ◽  
Sspe Virus ◽  
Specific Immunoglobulin ◽  
Antibody Titers ◽  
The Brain

Subacute sclerosing panencephalitis (SSPE) is a slowly progressing disease of the CNS in children which is caused by measles virus. Ferrets immunized with measles virus prior to inoculation with the cell associated, syncytiogenic D.R. strain of SSPE virus exhibit characteristics very similar to the human disease. Measles virus nucleocapsids are present, high measles antibody titers are found in the sera and inflammatory lesions are prominent in the brains. Measles virus specific immunoglobulin G (IgG) is present in the brain,and IgG/ albumin ratios indicate that the antibodies are synthesized within the CNS.

An Automated Procedure for the Determination of Ammonia in Tobacco

1972 ◽  
Vol 6 (4) ◽  
Author(s):  
P.F. Collins ◽  
W.W. Lawrence ◽  
J.F. Williams
Keyword(s):  
Standard Deviation ◽  
Relative Standard Deviation ◽  
Distillation Method ◽  
Relative Standard ◽  
Tobacco Sample ◽  
Automated Determination

AbstractA procedure for the automated determination of ammonia in tobacco has been developed. Ammonia is extracted from the ground tobacco sample with water and is determined with a Technicon Auto Analyser system which employs separation of the ammonia through volatilization followed by colourimetry using the phenate-hypochlorite reaction. The procedure has been applied to a variety of tobaccos containing from 0.02 to 0.5 % ammonia with an overall relative standard deviation of 2 %. The accuracy of the procedure as judged by recovery tests and by comparison to a manual distillation method is considered adequate

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