scholarly journals Comparison of Citrated Human Blood, Citrated Sheep Blood, and Defibrinated Sheep Blood Mueller-Hinton Agar Preparations for Antimicrobial Susceptibility Testing of Streptococcus pneumoniae Isolates

2010 ◽  
Vol 48 (10) ◽  
pp. 3770-3772 ◽  
Author(s):  
C. Satzke ◽  
A. Seduadua ◽  
R. Chandra ◽  
J. R. Carapetis ◽  
E. K. Mulholland ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Human Blood ◽  
Antimicrobial Susceptibility ◽  
Susceptibility Testing ◽  
Antimicrobial Susceptibility Testing ◽  
Defibrinated Sheep Blood ◽  
Sheep Blood ◽  
Mueller Hinton ◽  
Mueller Hinton Agar

scholarly journals Performance of Etest for Antimicrobial Susceptibility Testing of Abiotrophia defectiva and Granulicatella Species: TABLE 1

2016 ◽  
Vol 54 (8) ◽  
pp. 2194-2196 ◽  
Author(s):  
Michael O. Alberti ◽  
Janet A. Hindler ◽  
Romney M. Humphries
Keyword(s):  
Antimicrobial Susceptibility ◽  
Susceptibility Testing ◽  
Antimicrobial Susceptibility Testing ◽  
Broth Microdilution ◽  
Mueller Hinton ◽  
Abiotrophia Defectiva ◽  
Mueller Hinton Agar

The Etest on chocolate Mueller-Hinton agar was compared to broth microdilution (BMD) for 125 isolates of nutritionally variant streptococci. Vancomycin Etests yielded 31.1% essential agreement (EA) and 20.0% categorical agreement (CA). Penicillin Etests yielded 86.0% EA and 85.6% CA, whereas ceftriaxone Etests yielded 73.6% EA and 68.0% CA.

scholarly journals Optochin Revisited: Defining the Optimal Type of Blood Agar for Presumptive Identification of Streptococcus pneumoniae

1998 ◽  
Vol 36 (3) ◽  
pp. 833-834 ◽  
Author(s):  
M. A. Gardam ◽  
M. A. Miller
Keyword(s):  
Streptococcus Pneumoniae ◽  
Susceptibility Testing ◽  
Blood Agar ◽  
Sheep Blood ◽  
Mueller Hinton ◽  
Columbia Agar ◽  
Presumptive Identification ◽  
Mueller Hinton Agar ◽  
Inhibition Zones ◽  
Optimal Type

To determine the optimal media for optochin susceptibility testing of Streptococcus pneumoniae, we measured inhibition zones for 72 S. pneumoniae and 22 Streptococcus viridans isolates on three blood-containing media. Because 15.3, 0, and 22.2% of S. pneumoniae organisms were misidentified on Columbia agar, Trypticase soy agar (TSA), and Mueller-Hinton agar, respectively, each containing sheep blood, we recommend that TSA-sheep blood agar be used.

scholarly journals Determination of Trimethoprim-Sulfamethoxazole Resistance in Streptococcus pneumoniae by Using the E Test with Mueller-Hinton Agar Supplemented with Sheep or Horse Blood May Be Unreliable

1999 ◽  
Vol 37 (1) ◽  
pp. 215-217 ◽  
Author(s):  
M. Lovgren ◽  
L. Dell’Acqua ◽  
R. Palacio ◽  
G. Echániz-Aviles ◽  
A. Soto-Noguerón ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Clinical Laboratory ◽  
Reference Method ◽  
Error Rates ◽  
National Committee ◽  
Defibrinated Sheep Blood ◽  
Sheep Blood ◽  
Mueller Hinton ◽  
Mueller Hinton Agar ◽  
Horse Blood

An international, multicenter study compared trimethoprim-sulfamethoxazole MICs for 743 Streptococcus pneumoniae isolates (107 to 244 isolates per country) by E test, using Mueller-Hinton agar supplemented with 5% defibrinated horse blood or 5% defibrinated sheep blood, with MICs determined by the National Committee for Clinical Laboratory Standards broth microdilution reference method. Agreement within 1 log2dilution and minor error rates were 69.3 and 15.5%, respectively, on sheep blood-supplemented agar and 76.9 and 13.6%, respectively, with horse blood as the supplement. Significant interlaboratory variability was observed. E test may not be a reliable method for determining the resistance of pneumococci to trimethoprim-sulfamethoxazole.

Susceptibilities of invasive Neisseria meningitidis strains to agents used for prophylaxis and to penicillin G

2021 ◽  
pp. e20200034
Author(s):  
Carrie Phillips ◽  
David JM Haldane
Keyword(s):  
Cerebrospinal Fluid ◽  
Nova Scotia ◽  
Neisseria Meningitidis ◽  
Antimicrobial Susceptibility ◽  
Penicillin G ◽  
Minimal Inhibitory Concentrations ◽  
Sheep Blood ◽  
Mueller Hinton ◽  
Mueller Hinton Agar

Antimicrobial susceptibility of 50 Neisseria meningitidis strains detected in Nova Scotia between 2004 and 2018 was determined. The isolates were cultured from sites that might prompt chemoprophylaxis (27 blood, 18 cerebrospinal fluid [CSF], 3 CSF–blood, and 2 conjunctiva). Minimal inhibitory concentrations (MICs) were determined to azithromycin, ciprofloxacin, minocycline, rifampin, trimethoprim–sulfamethoxazole, and penicillin G, using a diffusion gradient strip on Mueller–Hinton agar with 5% sheep blood in 5% CO2 for 20–24 hours. All isolates remained susceptible to azithromycin, ciprofloxacin, minocycline, and rifampin, but there was 26% resistance to trimethoprim–sulfamethoxazole. There was a rise in penicillin MIC of the isolates over the study period.

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