scholarly journals Pulsed-Field Gel Electrophoresis Is More Efficient than Ribotyping and Random Amplified Polymorphic DNA Analysis in Discrimination of Pasteurella haemolytica Strains

1999 ◽  
Vol 37 (2) ◽  
pp. 380-385 ◽  
Author(s):  
Angeli Kodjo ◽  
Laurence Villard ◽  
Chantal Bizet ◽  
Jean-Louis Martel ◽  
Richard Sanchis ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Dna Analysis ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Geographic Origin ◽  
Pulsed Field Gel Electrophoresis ◽  
Pasteurella Haemolytica ◽  
Pulsed Field ◽  
Rapd Pattern ◽  
Almost All

One hundred thirty-three strains of Pasteurella haemolytica of both biotypes (90 and 43 strains of biotypes A and T, respectively) and almost all the serotypes were subjected to ribotyping, random amplified polymorphic DNA (RAPD) analysis, and pulsed-field gel electrophoresis (PFGE) analysis for epidemiological purposes. A total of 15 patterns recorded as ribotypes HA to HO were found for the P. haemolytica biotype A strains, with ribotypes HA, HC, and HD being encountered most often (66 strains [74%]); and 20 ribotypes, designated HA′ to HT′, that were clearly distinct from those observed for biotype A strains were observed for strains of biotype T. RAPD analysis generated a total of 44 (designated Rp1 to Rp44) and 15 (designated Rp1′ to Rp 15′) unique RAPD patterns for biogroup A and biogroup T, respectively. Analysis of the data indicated that a given combined ribotype-RAPD pattern could be observed for biotype A strains of different serotypes, whatever the zoological or geographic origin, whereas this was not the case for biotype T strains. PFGE appeared to be more efficient in strain discrimination since selected strains from various zoological or geographical origins harboring the same ribotype-RAPD group were further separated into unique entities.

scholarly journals Comparison of Ribotyping, Randomly Amplified Polymorphic DNA Analysis, and Pulsed-Field Gel Electrophoresis in Typing ofLactobacillus rhamnosus and L. caseiStrains

1999 ◽  
Vol 65 (9) ◽  
pp. 3908-3914 ◽  
Author(s):  
Soile Tynkkynen ◽  
Reetta Satokari ◽  
Maria Saarela ◽  
Tiina Mattila-Sandholm ◽  
Maija Saxelin
Keyword(s):  
Gel Electrophoresis ◽  
Type Strain ◽  
Dna Analysis ◽  
Rapd Analysis ◽  
Pulsed Field Gel Electrophoresis ◽  
Strain Atcc ◽  
Randomly Amplified Polymorphic Dna ◽  
Specific Primers ◽  
Pulsed Field ◽  
Species Specific

ABSTRACT A total of 24 strains, biochemically identified as members of theLactobacillus casei group, were identified by PCR with species-specific primers. The same set of strains was typed by randomly amplified polymorphic DNA (RAPD) analysis, ribotyping, and pulsed-field gel electrophoresis (PFGE) in order to compare the discriminatory power of the methods. Species-specific primers for L. rhamnosusand L. casei identified the type strain L. rhamnosus ATCC 7469 and the neotype strain L. caseiATCC 334, respectively, but did not give any signal with the recently revived species L. zeae, which contains the type strain ATCC 15820 and the strain ATCC 393, which was previously classified asL. casei. Our results are in accordance with the suggested new classification of the L. casei group. Altogether, 21 of the 24 strains studied were identified with the species-specific primers. In strain typing, PFGE was the most discriminatory method, revealing 17 genotypes for the 24 strains studied. Ribotyping and RAPD analysis yielded 15 and 12 genotypes, respectively.

scholarly journals Molecular typing of Mannheimia (Pasteurella) haemolytica serotype A1 isolates from cattle in Japan

2003 ◽  
Vol 131 (2) ◽  
pp. 939-946 ◽  
Author(s):  
K. KATSUDA ◽  
M. KOHMOTO ◽  
K. KAWASHIMA ◽  
H. TSUNEMITSU ◽  
T. TSUBOI ◽  
...  
Keyword(s):  
Molecular Typing ◽  
Gel Electrophoresis ◽  
Random Amplified Polymorphic Dna ◽  
Geographic Origin ◽  
Pulsed Field Gel Electrophoresis ◽  
Pasteurella Haemolytica ◽  
Type I ◽  
Banding Patterns ◽  
Molecular Profiles ◽  
Pfge Profiles

Pulsed field gel electrophoresis (PFGE) and random amplified polymorphic DNA (RAPD) methods were applied for molecular typing of 130 Mannheimia (Pasteurella) haemolytica serotype A1 isolates obtained from 13 prefectures in Japan. These isolates were divided into 15 ApaI PFGE profiles that formed six distinct clusters (clusters A–F). Fifty-three (40·7%) isolates were classified in cluster B, and 20·0, 13·8, 12·3, 6·9 and 6·1% of isolates were in clusters E, A, F, D and C, respectively. The isolates of cluster B were differentiated into seven subtypes (B1–B7) and subtype B5 contained 63% (34/53) of isolates. RAPD revealed four banding patterns (types I–IV), and among 130 isolates 60·7% (79/130) of isolates were RAPD type I. All of the RAPD type I isolates were grouped into clusters A–C by PFGE. There was no relationship between molecular typing and geographic origin of these isolates. These results indicate that isolates of M. haemolytica A1 strain with various molecular profiles have already spread in Japan and may have caused sporadic infections.

scholarly journals Molecular typing of Mycoplasma pneumoniae strains by PCR-based methods and pulsed-field gel electrophoresis. Application to French and Danish isolates

2000 ◽  
Vol 124 (1) ◽  
pp. 103-111 ◽  
Author(s):  
A. COUSIN-ALLERY ◽  
A. CHARRON ◽  
B. DE BARBEYRAC ◽  
G. FREMY ◽  
J. SKOV JENSEN ◽  
...  
Keyword(s):  
Mycoplasma Pneumoniae ◽  
Gel Electrophoresis ◽  
Random Amplified Polymorphic Dna ◽  
Rflp Analysis ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Group I ◽  
Additional Group ◽  
Group Ii ◽  
Almost All

Restriction fragment length polymorphism (RFLP) analysis of the amplified P1 gene was used to type 153 strains of Mycoplasma pneumoniae isolated in France between 1977 and 1994, and in Denmark between 1962 and 1994, and an additional group of 28 strains isolated from Belgium and Germany between 1990 and 1993. Random amplified polymorphic DNA (RAPD) analysis was tested on French, Belgian and German strains. Both methods separated the strains into two groups corresponding to the two reference strains M129 (group I) and FH (group II), and gave concordant results. When 75 selected strains of different geographical origin were analysed by pulsed-field gel electrophoresis (PFGE), strains of group II fell into two closely related subgroups, subgroup IIa corresponding to the reference strain FH, and subgroup IIb. Most of the strains isolated in Denmark in the period 1962–86 belonged to group I. Almost all strains isolated in France and Denmark between 1987 and 1988 were from group II, the two subgroups being present. In 1991–3, almost all strains from France as well as Denmark, Germany and Belgium belonged to group I.

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