scholarly journals Comparison of the Mycobacteria Growth Indicator Tube with MB Redox, Löwenstein-Jensen, and Middlebrook 7H11 Media for Recovery of Mycobacteria in Clinical Specimens

1999 ◽  
Vol 37 (5) ◽  
pp. 1366-1369 ◽  
Author(s):  
Ákos Somoskövi ◽  
Pál Magyar
Keyword(s):  
Nontuberculous Mycobacteria ◽  
Redox Systems ◽  
Clinical Specimens ◽  
Indicator Tube ◽  
Rate Of Recovery ◽  
The Mean ◽  
Smear Negative ◽  
Time To Detection ◽  
Mean Time ◽  
Growth Indicator

The rate of recovery and the mean time to detection of mycobacteria in clinical specimens were evaluated with two nonradiometric broth-based systems, the Mycobacteria Growth Indicator Tube (MGIT) and MB Redox systems. The data obtained for each system were compared with each other and with those obtained with the Löwenstein-Jensen (LJ) and Middlebrook 7H11 reference media. A total of 117 mycobacterial isolates (Mycobacterium tuberculosis, n = 112; nontuberculous mycobacteria, n = 5) were detected in 486 clinical specimens. The recovery rates for M. tuberculosis were 91 of 112 (81.3%) isolates with MGIT and 81 of 112 (72.3%) isolates with MB Redox. The combination of MGIT plus MB Redox recovered 104 of the 112 (92.9%) M. tuberculosisisolates. MGIT plus LJ plus Middlebrook 7H11 recovered 106 of the 112 (94.6%) isolates, MB Redox plus LJ plus Middlebrook 7H11 recovered 99 of the 112 (88.4%) isolates, and LJ plus Middlebrook 7H11 recovered 84 of the 112 (75.0%) isolates. The mean time to detection of M. tuberculosis in smear-positive specimens was 7.2 days with MGIT, 6.9 days with MB Redox, 20.4 days with LJ, and 17.6 days with Middlebrook 7H11. The mean time to detection of M. tuberculosis in smear-negative specimens was 19.1 days with MGIT, 15.5 days with MB Redox, 25.8 days with LJ, and 21.6 days with Middlebrook 7H11. The contamination rates were 4.4, 3.8, 2.1, and 2.7% for MGIT, MB Redox, LJ, and Middlebrook 7H11, respectively. In conclusion, MGIT and MB Redox can be viable tools in the routine mycobacteriology laboratory.

Evaluation of the BBL MGIT (Mycobacterial Growth Indicator Tube) AST SIRE System for Antimycobacterial Susceptibility Testing of Mycobacterium tuberculosis to 4 Primary Antituberculous Drugs

2000 ◽  
Vol 124 (1) ◽  
pp. 82-86
Author(s):  
John S. Bergmann ◽  
Geoffrey Fish ◽  
Gail L. Woods
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Susceptibility Testing ◽  
Clinical Isolates ◽  
Indicator Tube ◽  
The Mean ◽  
Inoculum Preparation ◽  
Mycobacterial Growth ◽  
Mean Time ◽  
Mycobacterial Growth Indicator Tube ◽  
Growth Indicator

Abstract Objective.—To evaluate the performance of the BBL MGIT (Mycobacterial Growth Indicator Tube) AST SIRE system for the antimycobacterial susceptibility testing of Mycobacterium tuberculosis to isoniazid (at a concentration equivalent to the lower concentration used for testing by the method of proportion), rifampin, ethambutol, and streptomycin. Design.—Thirty-one clinical isolates and 30 challenge strains provided by the Centers for Disease Control and Prevention (CDC) were tested by MGIT AST SIRE using 2 methods of inoculum preparation, and results were compared with those of the method of proportion, which was considered the reference method. Clinical isolates for which the results of the 2 methods were discordant also were tested at 2 reference laboratories. Results.—Based on data from our site and the reference laboratories, agreement rates between initial MGIT AST SIRE results and the method of proportion for the clinical isolates with the inoculum prepared from a McFarland equivalent and from a positive MGIT tube, respectively, were 100% and 96.8% for isoniazid, 100% and 100% for rifampin, 96.8% and 100% for ethambutol, and 100% and 100% for streptomycin, excluding the isolate for which the discordant streptomycin result could not be resolved. For the 30 challenge isolates, agreement rates between MGIT AST SIRE and expected results and between method of proportion and expected results, respectively, were 96.7% and 93.3% for isoniazid, 93.3% and 100% for rifampin, 83.3% and 100% for ethambutol, and 93.3% and 100% for streptomycin. For the clinical isolates, the mean time to an MGIT AST SIRE result of susceptible was 6.15 ± 0.13 days (range, 5–8 days). For a result of resistant, the mean time overall was 5.00 ± 0.24 days (range, 3–8 days). Conclusion.—These data suggest that the MGIT AST SIRE system, using either method of inoculum preparation, is an acceptable alternative to the BACTEC 460 TB method of susceptibility testing of clinical isolates of M tuberculosis to isoniazid, rifampin, ethambutol, and streptomycin. Reasons for the lower agreement with the CDC challenge isolates should be investigated. Further evaluation of the MGIT AST SIRE system using a concentration of isoniazid equivalent to the higher concentration tested by the method of proportion would be useful, because the decision concerning use of this agent generally is based on the susceptibility test result at the higher concentration.

Comparison of the BBL mycobacteria growth indicator tube, the BACTEC 12B, and solid media for the isolation of Mycobacterium bovis

2017 ◽  
Vol 29 (4) ◽  
pp. 508-512 ◽  
Author(s):  
Gary F. Yates ◽  
Marian Price-Carter ◽  
Kirstie Bland ◽  
Maree A. Joyce ◽  
Farina Khan ◽  
...  
Keyword(s):  
New Zealand ◽  
Mycobacterium Bovis ◽  
Tandem Repeats ◽  
Variable Number ◽  
Tissue Samples ◽  
The North ◽  
Indicator Tube ◽  
The Mean ◽  
Mean Time ◽  
Growth Indicator

We compared different methods for their ability to isolate Mycobacterium bovis from tissue samples from animals with lesions resembling bovine tuberculosis. In the first trial, M. bovis was isolated from 86 of 200 tissue samples that were cultured using 2 liquid media, BACTEC 12B and BBL mycobacteria growth indicator tube (MGIT), and a solid medium, Middlebrook 7H11 supplemented with pyruvate (7H11P). M. bovis was isolated from 2 samples with MGIT but not BACTEC 12B. M. bovis was isolated from 9 samples with BACTEC but not MGIT; these 9 samples came from the North Canterbury/Marlborough region of New Zealand. The proportion of tissues from which M. bovis was isolated with BACTEC 12B or MGIT and the mean time for isolation was different for samples from the North Canterbury/Marlborough region but not the rest of New Zealand. In the second trial, M. bovis was isolated from 401 of 1,033 tissues that were cultured using MGIT, Middlebrook 7H9 broth, or solid 7H11P. The proportion of isolates of M. bovis and the mean time for their isolation with MGIT was different for the North Canterbury/Marlborough and the rest of New Zealand. The reason for this difference was not determined but may be related to the genotypes present in this region. Genotyping using variable number tandem repeats (VNTRs) of 197 isolates of M. bovis revealed that the 44 isolates from North Canterbury/Marlborough were represented by 2 closely related VNTR types that were not found in 153 isolates from the remainder of New Zealand.

scholarly journals Comparison of recoveries of Mycobacterium tuberculosis using the automated BACTEC MGIT 960 System and Lowenstein – Jensen medium

2010 ◽  
Vol 9 (1) ◽  
pp. 44
Author(s):  
Mo. A.AL-Mazini, T. Bukeet, and A. Abdul Kareem
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Recovery Rates ◽  
Clinical Specimens ◽  
Indicator Tube ◽  
Lowenstein Jensen ◽  
Bactec System ◽  
Smear Negative ◽  
The Difference ◽  
Growth Indicator

We examined whether the BACTEC/ Mycobacteria Growth Indicator Tube (MGIT) System alone could supplant the use of a supplemental Lowenstein–Jensen (LJ) slant for routine recovery of M. tuberculosis from clinical specimens. A total of 392 specimens of sputum were included in the study, collected from 196 patients. Specimens were processed with standard N-acetyl- L- Cysteine (NALC-NaOH) method, then inoculated onto BACTEC MGIT 960 and onto LJ media. The recovery rates of M.tuberculosis were 100 % (256/256) with BACTEC MGIT 960 and 72.6%(186/256) with LJ. The rates of contamination for each of the system were 4.8%with BACTEC MGIT 960 and 5.3%with LJ. The TTD for M. tuberculosis was 11.3 days with BACTEC System and 30.8 days with LJ. The difference in TTD between smear positive and smear negative specimens for M.tuberculosis with BACTEC MGIT 960 was not statistically significant. This study shows that the BACTEC System demonstrates better sensitivity for the recovery of M. tuberculosis from clinical specimens.

scholarly journals Evaluation of Mycobacteria Growth Indicator Tube (MGIT), an Automated Culture System for Detection of Mycobacteria from Clinical Specimens

1999 ◽  
Vol 73 (2) ◽  
pp. 172-178 ◽  
Author(s):  
Intetsu KOBAYASHI ◽  
Haruyo TODA ◽  
Etsuko KOYAMA ◽  
Miyuki HASEGAWA ◽  
Norishige HASHIGUCHI ◽  
...  
Keyword(s):  
Culture System ◽  
Clinical Specimens ◽  
Indicator Tube ◽  
Growth Indicator

scholarly journals Evaluation of the New MB Redox System for Detection of Growth of Mycobacteria

1999 ◽  
Vol 37 (6) ◽  
pp. 2013-2015 ◽  
Author(s):  
Emmanuelle Cambau ◽  
Claudine Wichlacz ◽  
Chantal Truffot-Pernot ◽  
Vincent Jarlier
Keyword(s):  
Recovery Rate ◽  
Culture System ◽  
Nontuberculous Mycobacteria ◽  
Redox System ◽  
Mycobacterial Culture ◽  
The Mean ◽  
Time To Detection ◽  
Respiratory Specimens

We evaluated a new mycobacterial culture system, MB Redox, for recovery rate and time to detection of mycobacteria from 742 consecutive respiratory specimens and compared the results to those found with Löwenstein-Jensen (LJ) medium. Twenty specimens (2.7%) were positive for M. tuberculosis: 17 on LJ medium and 19 in MB Redox, with 16 specimens positive in both media. In addition, 24 specimens (3.2%) were positive for nontuberculous mycobacteria (NTM), 20 on LJ medium, 18 in MB Redox, and 14 in both media. For M. tuberculosis, the mean times to detection were 28.9 days on LJ medium and 23.6 days in MB Redox, and for NTM, the mean times to detection were 40.6 days on LJ medium and 32.3 days in MB Redox.

scholarly journals Comparison of mycobacteria growth indicator tube with BACTEC 460 for detection and recovery of mycobacteria from clinical specimens.

1996 ◽  
Vol 34 (9) ◽  
pp. 2236-2239 ◽  
Author(s):  
F Z Badak ◽  
D L Kiska ◽  
S Setterquist ◽  
C Hartley ◽  
M A O'Connell ◽  
...  
Keyword(s):  
Clinical Specimens ◽  
Indicator Tube ◽  
Growth Indicator

scholarly journals The time of detection of recessive visible genes in small populations

1978 ◽  
Vol 31 (3) ◽  
pp. 255-264 ◽  
Author(s):  
Alan Robertson
Keyword(s):  
Artificial Selection ◽  
Finite Population ◽  
Random Mating ◽  
Recessive Gene ◽  
New Mutation ◽  
Mating Population ◽  
Initial Generation ◽  
The Mean ◽  
Time To Detection ◽  
Mean Time

SUMMARYHomozygotes for recessive visible genes have often been discovered in lines under artificial selection, sometimes many generations from the start. As a help in the interpretation of this phenomenon, the distribution of the time to first detection as a homozygote of a recessive gene occurring only once in the initial generation has been obtained. Alternatively the results may be considered as referring to the time of first appearance as a homozygote of a new mutation occurring in a finite population. For a monoecious random mating population of size N with selfing permitted, the mean time to detection is very close to 2N⅓ over a range of N from 1 to 500 with a coefficient of variation of roughly 2/3 and a 95% upper limit about 2·5 times the mean. If selfing is prohibited, the mean time is increased by a little over 1 generation. The treatment is extended to cover the effects of artificial selection in favour of the heterozygote, of the frequency of occurrence in the initial generation and of the examination of more individuals each generation than are used as parents.

scholarly journals Compare the Effects of Epinephrine and Vasopressin in Return of Spontaneous Circulation

2018 ◽  
Vol 6 (3) ◽  
pp. 7
Author(s):  
Samad Shams Vahdati ◽  
Azra Nejabatian ◽  
Farzad Rahmani ◽  
Paria Habibollahi ◽  
Pegah Sepehri Majd
Keyword(s):  
Cardiopulmonary Arrest ◽  
Coronary Perfusion Pressure ◽  
Spontaneous Circulation ◽  
P Value ◽  
Coronary Perfusion ◽  
Results Of Treatment ◽  
Significant Difference ◽  
Rate Of Recovery ◽  
The Mean ◽  
Mean Time

Background: There is a conflict in the superiority of each of the vasopressin and epinephrine compared to the other. Vasopressin has a vasoconstrictive action that results in an increase of the coronary perfusion pressure. Due to the expensive and sometimes scarce of vasopressin in most hospitals, this study aims to evaluate the response rate of vasopressin compared with epinephrine, in return of ROSC. Methods: In this descriptive-analytical study all patients in the emergency medicine department were enrolled in the study suffered a cardiopulmonary arrest and resuscitation will be done instantly for them (According to the guidelines AHA 2010). Their data were extracted from the hospital records and the success rate of recovery, 3-month survival and complications in patients recovering from the drug used during the CPR were analyzed. Results: A total of 61 patients record were analyzed. 31 patients had received epinephrine alone and 30 patients received a combination of epinephrine and vasopressin. No significant difference was observed between the two groups in terms of sex, sepsis, hypovolemia, renal failure, cancers, drug toxicity, brady, dysrhythmia, PEA, VT, VF, defibrillator, duration of CPR and three month outcome. The mean time of CPR in combination of epinephrine and vasopressin group was 27.26±12.72 and the mean time of CPR in epinephrine group was 27.24±13.510 (p-value= 0.99).Conclusion: Among patients with in-hospital cardiopulmonary arrest in this study no statistically significant difference was obtained between the results of treatment with epinephrine alone and combination of epinephrine and vasopressin.

scholarly journals Evaluation of BACTEC MYCO/F Lytic Medium for Recovery of Mycobacteria and Fungi from Blood

1998 ◽  
Vol 36 (5) ◽  
pp. 1176-1179 ◽  
Author(s):  
Rebecca T. Waite ◽  
Gail L. Woods
Keyword(s):  
Blood Culture ◽  
Culture System ◽  
Histoplasma Capsulatum ◽  
Mycobacterium Kansasii ◽  
Fungal Isolation ◽  
Blood Culture System ◽  
Valid Assessment ◽  
The Mean ◽  
Time To Detection ◽  
Mean Time

The reliability of MYCO/F Lytic medium in the BACTEC 9240 blood culture system was evaluated by comparing its performance to that of the Isolator system for the recovery of fungi and to that of the ESP II system for the recovery of mycobacteria. Of 717 specimens of blood cultured for fungi, 24 were positive; 12 samples were positive with both systems, 7 samples were positive with the Isolator system only, and 5 samples were positive with MYCO/F Lytic medium only. Fourteen samples grew Histoplasma capsulatum; both systems detectedH. capsulatum in seven samples but the Isolator system alone detected H. capsulatum in seven samples. The mean times to the detection of H. capsulatum were 8 days (range, 4 to 13 days) for MYCO/F Lytic medium and 9 days (range, 6 to 18 days) for the Isolator system; the mean times to identification were 20 days (range, 15 to 24 days) for isolates recovered with MYCO/F Lytic medium and 11 days (range, 6 to 18 days) for those recovered with the Isolator system (P < 0.05). Cryptococcus neoformans was isolated from 10 fungal cultures; five isolates grew in both systems, and five isolates grew in MYCO/F Lytic medium only. The mean times to detection of C. neoformans were 4 days (range, 2 to 6 days) for MYCO/F Lytic medium and 7 days (range, 5 to 7 days) for the Isolator system (P < 0.05); the mean times to identification were 15 days (range, 7 to 27 days) for isolates recovered with MYCO/F Lytic medium and 8 days (range, 7 to 11 days) for those recovered with the Isolator system. Of the 687 samples of blood cultured for mycobacteria, 64 blood samples from 42 patients grew mycobacteria (58 grew Mycobacterium avium complex, 4 grew Mycobacterium kansasii, and 2 grew Mycobacterium tuberculosis); 42 isolates were recovered with both systems, 18 were isolated with MYCO/F medium only, and 4 were isolated with the ESP II system only alone (P < 0.05). The mean time to detection of mycobacteria with MYCO/F Lytic medium was 14 days, whereas it was 17 days with the ESP II system (P < 0.05). In summary, the combination of MYCO/F Lytic medium and the BACTEC 9240 instrument is an excellent blood culture system for the growth and detection of mycobacteria. A valid assessment of MYCO/F Lytic medium with regard to fungal isolation, however, was not possible due to the small number of isolates recovered.

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