Comparison of the BBL mycobacteria growth indicator tube, the BACTEC 12B, and solid media for the isolation of Mycobacterium bovis

2017 ◽  
Vol 29 (4) ◽  
pp. 508-512 ◽  
Author(s):  
Gary F. Yates ◽  
Marian Price-Carter ◽  
Kirstie Bland ◽  
Maree A. Joyce ◽  
Farina Khan ◽  
...  
Keyword(s):  
New Zealand ◽  
Mycobacterium Bovis ◽  
Tandem Repeats ◽  
Variable Number ◽  
Tissue Samples ◽  
The North ◽  
Indicator Tube ◽  
The Mean ◽  
Mean Time ◽  
Growth Indicator

We compared different methods for their ability to isolate Mycobacterium bovis from tissue samples from animals with lesions resembling bovine tuberculosis. In the first trial, M. bovis was isolated from 86 of 200 tissue samples that were cultured using 2 liquid media, BACTEC 12B and BBL mycobacteria growth indicator tube (MGIT), and a solid medium, Middlebrook 7H11 supplemented with pyruvate (7H11P). M. bovis was isolated from 2 samples with MGIT but not BACTEC 12B. M. bovis was isolated from 9 samples with BACTEC but not MGIT; these 9 samples came from the North Canterbury/Marlborough region of New Zealand. The proportion of tissues from which M. bovis was isolated with BACTEC 12B or MGIT and the mean time for isolation was different for samples from the North Canterbury/Marlborough region but not the rest of New Zealand. In the second trial, M. bovis was isolated from 401 of 1,033 tissues that were cultured using MGIT, Middlebrook 7H9 broth, or solid 7H11P. The proportion of isolates of M. bovis and the mean time for their isolation with MGIT was different for the North Canterbury/Marlborough and the rest of New Zealand. The reason for this difference was not determined but may be related to the genotypes present in this region. Genotyping using variable number tandem repeats (VNTRs) of 197 isolates of M. bovis revealed that the 44 isolates from North Canterbury/Marlborough were represented by 2 closely related VNTR types that were not found in 153 isolates from the remainder of New Zealand.

Evaluation of the BBL MGIT (Mycobacterial Growth Indicator Tube) AST SIRE System for Antimycobacterial Susceptibility Testing of Mycobacterium tuberculosis to 4 Primary Antituberculous Drugs

2000 ◽  
Vol 124 (1) ◽  
pp. 82-86
Author(s):  
John S. Bergmann ◽  
Geoffrey Fish ◽  
Gail L. Woods
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Susceptibility Testing ◽  
Clinical Isolates ◽  
Indicator Tube ◽  
The Mean ◽  
Inoculum Preparation ◽  
Mycobacterial Growth ◽  
Mean Time ◽  
Mycobacterial Growth Indicator Tube ◽  
Growth Indicator

Abstract Objective.—To evaluate the performance of the BBL MGIT (Mycobacterial Growth Indicator Tube) AST SIRE system for the antimycobacterial susceptibility testing of Mycobacterium tuberculosis to isoniazid (at a concentration equivalent to the lower concentration used for testing by the method of proportion), rifampin, ethambutol, and streptomycin. Design.—Thirty-one clinical isolates and 30 challenge strains provided by the Centers for Disease Control and Prevention (CDC) were tested by MGIT AST SIRE using 2 methods of inoculum preparation, and results were compared with those of the method of proportion, which was considered the reference method. Clinical isolates for which the results of the 2 methods were discordant also were tested at 2 reference laboratories. Results.—Based on data from our site and the reference laboratories, agreement rates between initial MGIT AST SIRE results and the method of proportion for the clinical isolates with the inoculum prepared from a McFarland equivalent and from a positive MGIT tube, respectively, were 100% and 96.8% for isoniazid, 100% and 100% for rifampin, 96.8% and 100% for ethambutol, and 100% and 100% for streptomycin, excluding the isolate for which the discordant streptomycin result could not be resolved. For the 30 challenge isolates, agreement rates between MGIT AST SIRE and expected results and between method of proportion and expected results, respectively, were 96.7% and 93.3% for isoniazid, 93.3% and 100% for rifampin, 83.3% and 100% for ethambutol, and 93.3% and 100% for streptomycin. For the clinical isolates, the mean time to an MGIT AST SIRE result of susceptible was 6.15 ± 0.13 days (range, 5–8 days). For a result of resistant, the mean time overall was 5.00 ± 0.24 days (range, 3–8 days). Conclusion.—These data suggest that the MGIT AST SIRE system, using either method of inoculum preparation, is an acceptable alternative to the BACTEC 460 TB method of susceptibility testing of clinical isolates of M tuberculosis to isoniazid, rifampin, ethambutol, and streptomycin. Reasons for the lower agreement with the CDC challenge isolates should be investigated. Further evaluation of the MGIT AST SIRE system using a concentration of isoniazid equivalent to the higher concentration tested by the method of proportion would be useful, because the decision concerning use of this agent generally is based on the susceptibility test result at the higher concentration.

scholarly journals Comparison of the Mycobacteria Growth Indicator Tube with MB Redox, Löwenstein-Jensen, and Middlebrook 7H11 Media for Recovery of Mycobacteria in Clinical Specimens

1999 ◽  
Vol 37 (5) ◽  
pp. 1366-1369 ◽  
Author(s):  
Ákos Somoskövi ◽  
Pál Magyar
Keyword(s):  
Nontuberculous Mycobacteria ◽  
Redox Systems ◽  
Clinical Specimens ◽  
Indicator Tube ◽  
Rate Of Recovery ◽  
The Mean ◽  
Smear Negative ◽  
Time To Detection ◽  
Mean Time ◽  
Growth Indicator

The rate of recovery and the mean time to detection of mycobacteria in clinical specimens were evaluated with two nonradiometric broth-based systems, the Mycobacteria Growth Indicator Tube (MGIT) and MB Redox systems. The data obtained for each system were compared with each other and with those obtained with the Löwenstein-Jensen (LJ) and Middlebrook 7H11 reference media. A total of 117 mycobacterial isolates (Mycobacterium tuberculosis, n = 112; nontuberculous mycobacteria, n = 5) were detected in 486 clinical specimens. The recovery rates for M. tuberculosis were 91 of 112 (81.3%) isolates with MGIT and 81 of 112 (72.3%) isolates with MB Redox. The combination of MGIT plus MB Redox recovered 104 of the 112 (92.9%) M. tuberculosisisolates. MGIT plus LJ plus Middlebrook 7H11 recovered 106 of the 112 (94.6%) isolates, MB Redox plus LJ plus Middlebrook 7H11 recovered 99 of the 112 (88.4%) isolates, and LJ plus Middlebrook 7H11 recovered 84 of the 112 (75.0%) isolates. The mean time to detection of M. tuberculosis in smear-positive specimens was 7.2 days with MGIT, 6.9 days with MB Redox, 20.4 days with LJ, and 17.6 days with Middlebrook 7H11. The mean time to detection of M. tuberculosis in smear-negative specimens was 19.1 days with MGIT, 15.5 days with MB Redox, 25.8 days with LJ, and 21.6 days with Middlebrook 7H11. The contamination rates were 4.4, 3.8, 2.1, and 2.7% for MGIT, MB Redox, LJ, and Middlebrook 7H11, respectively. In conclusion, MGIT and MB Redox can be viable tools in the routine mycobacteriology laboratory.

On the current enhancement at the edge of a crack in a lattice of resistors

1998 ◽  
Vol 30 (2) ◽  
pp. 342-364 ◽  
Author(s):  
Howard M. Taylor ◽  
Dennis E. Sweitzer
Keyword(s):  
Random Walk ◽  
Vertical Direction ◽  
Potential Gradient ◽  
Domain Of Attraction ◽  
Lattice Points ◽  
Fourier Methods ◽  
The North ◽  
The Mean ◽  
Mean Time ◽  
Cauchy Process

Consider a network whose nodes are the integer lattice points and whose arcs are fuses of 1Ω resistance. Remove a horizontal segment ofNadjacent vertical arcs, forming a ‘crack’ of lengthN. Subject the network to a uniform potential gradient ofvvolts per arc in the north-south (or vertical) direction and measure the current in one of the two vertical arcs at the ends of the crack. We write this current in the forme(N)v, and calle(N) thecurrent enhancement.We show that the enhancement grows at a rate that is the order of the square root of the crack length. Our method is to identify the enhancement with the mean time to exit an interval for a certain integer valued random walk, and then to use some of the well-known Fourier methods for studying random walk. Our random walk has no mean or higher moments and is in the domain of attraction of the Cauchy law. We provide a good approximation to the enhancement using the explicitly known mean time to exit an interval for a Cauchy process. Weak convergence arguments together with an estimate of a recurrence probability enable us to show that the current in an intact fuse, that is in the interior of a crack of lengthN, grows p roportionally withN/logN.

Phocanema decipiens (Nematoda: Anisakinae): experimental infections in marine copepods

1982 ◽  
Vol 60 (4) ◽  
pp. 502-509 ◽  
Author(s):  
Gary McClelland
Keyword(s):  
Gravid Female ◽  
Mature Female ◽  
Cyclopoid Copepod ◽  
Calanoid Copepods ◽  
North West ◽  
The North ◽  
The Family ◽  
The Mean ◽  
Mean Time ◽  
Marine Copepods

Gravid female Phocanema decipiens were recovered from the stomachs of freshly killed harbour (Phoca vitulina) and grey seals (Halichoerus grypus). Ova dissected from the nematodes were incubated in seawater and the mean time to hatch varied from 8 days at 20 °C to 52 days at 5 °C. Posthatch survival of ensheathed larvae in seawater varied from 48 h at 20 °C to 140 days at 5 °C. Fifteen species of copepods collected from the North West Arm, Halifax, N.S., were exposed to freshly hatched larvae of P. decipiens. Exsheathed larvae of the parasite were subsequently detected in the haemocoel of 12 harpacticoid species including Danielsennia typica, Tisbe furcata, Ameira longipes, Enhydrosoma curticauda, and various undescribed species of the genera Halectinosoma, Tisbe, Alteutha, and Phyllothallestris and the family Diosaccidae. Phocanema decipiens also occurred in a cyclopoid copepod (Paracyclopina sp.) but calanoid copepods (Eurytemora sp. and Pseudocalanus sp.) did not become infected. The heaviest infections occurred in mature female copepods and prevalence and intensity of infection varied with host species. Adult male copepods and fifth copepodite females were lightly infected. Infected copepods survived for 3–7 days at 15 °C, 10–15 days at 10 °C, and 20–35 days at 5 °C. During the course of infection, larval P. decipiens grew an average of 60% and maximum of 130% in length but underwent little or no morphological change.

scholarly journals Reverse zoonotic tuberculosis transmission from an emerging Uganda I strain between pastoralists and cattle in South-Eastern Nigeria

2019 ◽  
Vol 15 (1) ◽  
Author(s):  
Hezekiah Kehinde Adesokan ◽  
Victor Oluwatoyin Akinseye ◽  
Elizabeth Maria Streicher ◽  
Paul Van Helden ◽  
Rob Mark Warren ◽  
...  
Keyword(s):  
Public Health ◽  
Mycobacterium Bovis ◽  
Latin American ◽  
Disease Burden ◽  
Tandem Repeats ◽  
Variable Number ◽  
Molecular Tools ◽  
Tuberculosis Transmission ◽  
Public Health Challenge ◽  
Variable Number Tandem Repeats

Abstract Background Tuberculosis remains a major public health challenge globally with increasing risks for inter-transmission between pastoralists and cattle in Nigeria. This study was aimed at using molecular tools to establish zoonotic transmission of tuberculosis between pastoralists and their cattle in Ebonyi State, Nigeria. Sputum (n = 149) and milk (n = 144) samples from pastoralists and cattle, respectively were screened on the assumption of subclinical infections considering unguarded human-livestock interactions. Isolates obtained were analysed using deletion typing, spoligotyping and 24-Mycobacterial Interspersed Repetitive Unit-Variable Number Tandem Repeats (MIRU-VNTR). Results Fifty-four MTC were confirmed by deletion typing and were differentiated accordingly (M. tuberculosis: pastoralists =42, cattle = 2; M. bovis: pastoralists =1; M. africanum: pastoralists =9). Spoligotyping indicated 59.2% Uganda I/SIT46 (pastoralists =28; cattle = 1), 16.3% Latin American Mediterranean/SIT61 (pastoralists =8), 2.0% T/SIT53 (pastoralists =1) strains of M. tuberculosis and new strains of M. bovis and M. africanum. The 24-MIRU-VNTR of selected predominant cluster isolates shared by cattle and pastoralists (Uganda I/SIT46: pastoralists =9; cattle = 1) showed the same number of copies at each of the repetitive loci. Conclusions Mycobacterium bovis was confirmed in humans and a reverse zoonotic tuberculosis transmission from an emerging Uganda I M. tuberculosis strain between pastoralists and cattle in Nigeria evidenced by MIRU-VNTR. Using molecular tools will help mitigate disease burden through informed epidemiological insights.

scholarly journals Metabolic fingerprints of Mycobacterium bovis cluster with molecular type: implications for genotype–phenotype links

Microbiology ◽  
2006 ◽  
Vol 152 (9) ◽  
pp. 2757-2765 ◽  
Author(s):  
Catherine L. Winder ◽  
Stephen V. Gordon ◽  
James Dale ◽  
R. Glyn Hewinson ◽  
Royston Goodacre
Keyword(s):  
Cluster Analysis ◽  
Mycobacterium Bovis ◽  
Tandem Repeats ◽  
Hierarchical Cluster ◽  
Variable Number ◽  
Resolving Power ◽  
Ft Ir ◽  
The Uk ◽  
Variable Number Tandem Repeats ◽  
High Degree

Mycobacterium bovis is the causative agent of bovine tuberculosis. Various genetic typing techniques have been used to trace the reservoirs of infection; however, they have limited success in population genetics and outbreak studies. Fourier-transform infrared spectroscopy (FT-IR) is a rapid phenotypic typing technique, which may be used to generate a metabolic fingerprinting and is increasingly used to characterize bacteria. When coupled with multivariate cluster analysis, this powerful combination has sufficient resolving power to discriminate bacteria down to subspecies level; however, to date this method has not been used in the differentiation of mycobacteria. Multiple isolates of the ten major spoligotypes in the UK, recovered from different geographical locations, were analysed using FT-IR. Hierarchical cluster analysis of the spectra showed that the isolates could be differentiated according to their spoligotypes. Six of the spoligotype FT-IR clusters were very homogeneous and all isolates were recovered together. However, the remaining four groups displayed a more heterogeneous phenotype, which may reflect greater variation than previously suspected within these groups. Included in the ten spoligotypes are the two most dominant isolates in the UK, designated types 9 and 17. Whilst type 17 showed a highly conserved phenotype as judged by FT-IR, type 9 showed a very heterogeneous metabolic profile and isolates were recovered throughout the dendrogram. This variation in type 9 is reflected in the high degree of diversity observed by variable number tandem repeats (VNTR) analysis, underlining the exquisite resolving power of FT-IR.

scholarly journals Anthropometric and clinical profiles of post-bariatric patients submitted to procedures in plastic surgery

2018 ◽  
Vol 45 (2) ◽  
Author(s):  
SIMONE CORRÊA ROSA ◽  
JEFFERSON LESSA SOARES DE MACEDO ◽  
LUIZ AUGUSTO CASULARI ◽  
LUCAS RIBEIRO CANEDO ◽  
JOÃO VITOR ALMEIDA MARQUES
Keyword(s):  
Postoperative Complications ◽  
Plastic Surgery ◽  
Analytical Study ◽  
The North ◽  
Bariatric Patients ◽  
Clinical Profiles ◽  
The Mean ◽  
Epidemiological Profile ◽  
Mean Time

ABSTRACT Objective: to evaluate the profile of patients submitted to post-bariatric plastic surgery at the North Wing Regional Hospital, Brasília, DF. Methods: we conducted a prospective, descriptive and analytical study of patients submitted to Roux-en-Y gastroplasty, and subsequently to plastic surgery, from January 2011 to December 2016. We assessed body mass index before gastroplasty and after surgery plastic surgery, postoperative complications and comorbidities. Results: we studied 139 patients (130 women and nine men), with a mean age of 41 years, who underwent 233 operations. The mean BMI at the time of plastic surgery was 27.44kg/m2. The mean weight loss was 47.02kg and the mean maximum BMI was 45.17kg/m2. The mean time between bariatric surgery and plastic surgery was 42 months. The most important co-morbidities before plastic surgery were arterial hypertension (11.5%), arthropathy (5.4%), diabetes mellitus (5%) and metabolic syndrome (4.3%) (p<0.01). Of the 139 patients operated on, 76.97% underwent abdominoplasty followed by mammoplasty (42.46%), rhytidoplasty (17.27%) and brachioplasty (13.67%). Fourteen (13.08%) patients underwent herniorrhaphy combined with abdominoplasty. We performed anchor abdominoplasty in 19.42%. The rate of postoperative complications was 26.65%. Conclusion: the epidemiological profile of post-bariatric patients who underwent plastic surgery was similar to that reported in the literature, except for the low rate of associated surgeries and postoperative complications. Plastic surgery in post-bariatric patients has led to an improvement in the quality of life in most of these patients.

Disruption of Light-Brown Apple Moth Epiphyas-Postvittana (Walker) (Lepidoptera, Tortricidae) Trapping in Nelson, New-Zealand

1990 ◽  
Vol 38 (4) ◽  
pp. 363 ◽  
Author(s):  
DM Suckling ◽  
DJ Rogers ◽  
PW Shaw
Keyword(s):  
New Zealand ◽  
Retention Rate ◽  
Light Brown Apple Moth ◽  
Pheromone Components ◽  
Tetradecenyl Acetate ◽  
Previously Treated ◽  
The Mean ◽  
Dispersal Distances ◽  
Lightbrown Apple Moth ◽  
Mean Time

Disruption of lightbrown apple moth trapping was tested as a prelude to assessment of mating dis- ruption; polyethylene dispensers releasing the known sex pheromone components, (E)-11-tetradecenyl acetate and (E,E)-9,ll-tetradecadien-l-yl acetate, at an estimated rate of 25 mg ha-' per hour in apple orchards in Nelson, New Zealand. A total of 2953 wild and 850 laboratory-reared males were marked and released at 16 sites in two pheromone-treated and two untreated blocks. No captures were recorded in sticky traps baited with virgin females or synthetic pheromones in pheromone-treated blocks, but 455 males were caught in untreated blocks, indicating complete prevention of trapping in the presence of the dispensers releasing synthetic pheromone. Individual dispensers released an estimated 4000-fold more pheromone per hour than females. Recapture of wild and laboratory-reared males averaged 28% and 31% respectively, in untreated blocks and surrounding traps. Removal of dispensers resulted in renewed capture inside previously treated blocks. Dispersal distances of wild and laboratory-reared males were not significantly different inside untreated blocks, but the mean time taken before recapture was twice as great for laboratory-reared moths (P<O.05). Wild males showed a greater tendency to disperse out of blocks (P<O.05). Dispersal distances exceeded 400 m for several moths. The daily survival or retention rate of wild moths inside release blocks was estimated to be 63%, although this rose to 74% when external traps were included. A maximum age of 19 days was recorded. Female moths mated with equal frequency when caged with males in pheromone-treated or untreated blocks.

scholarly journals Molecular characterisation of Mycobacterium bovis isolated from African buffaloes (Syncerus caffer) in Hluhluwe-iMfolozi Park in KwaZulu-Natal, South Africa

2011 ◽  
Vol 78 (1) ◽  
Author(s):  
Tiny M. Hlokwe ◽  
Akinbowale O. Jenkins ◽  
Elizabeth M. Streicher ◽  
Estelle H. Venter ◽  
Dave Cooper ◽  
...  
Keyword(s):  
South African ◽  
Mycobacterium Bovis ◽  
Tandem Repeats ◽  
Genetic Relationships ◽  
Variable Number ◽  
Syncerus Caffer ◽  
Kwazulu Natal ◽  
Typing Methods ◽  
Genetic Profiles ◽  
Reservoir Hosts

Bovine tuberculosis (BTB), a chronic disease of mammals caused by Mycobacterium bovis, is a threat to South African wildlife. It has been reported that African buffaloes (Syncerus caffer) are reservoir hosts of BTB in South African wildlife populations. This study reports on the molecular identification and typing of 31 M. bovis isolates collected between 1993 and 2008, mainly from buffaloes but also from two lions and a bush pig, in the Hluhluwe-iMfolozi Park (HiP) in KwaZulu-Natal. To study the dynamics of BTB in the buffalo populations, 28 M. bovis isolates from the HiP and epidemiologically related parks were characterised using regions of difference deletion analysis for species identification and spoligotyping, variable number of tandem repeats (VNTR), polymorphic G–C-rich sequences and IS6110 restriction fragment length polymorphism (RFLP) genotyping methods. At least three distinct M. bovis genotypes were found amongst HiP samples. The combination of VNTR typing (using a 16-loci panel) and IS6110 RFLP revealed the presence of three additional genetic profiles in individual buffaloes, demonstrating that the highest level of discrimination was achieved by these typing methods. One of the observed spoligotypes (SB0130) was dominant and represented 75% of isolates from buffaloes. A novel M. bovis spoligotype (SB1474), which is reported for the first time in this study, was observed in 14.3% of isolates from buffaloes. Based on the observed genetic relationships, the findings suggest independent introductions from at least three unrelated sources. These findings improve the knowledge regarding the diversity of circulating M. bovis strains in the HiP.

scholarly journals Characterisation of Neisseria meningitidis C strains causing two clusters in the north of Italy in 2007 and 2008

2009 ◽  
Vol 14 (16) ◽  
Author(s):  
C Fazio ◽  
A Neri ◽  
T Sofia ◽  
A Carannante ◽  
M G Caporali ◽  
...  
Keyword(s):  
Tandem Repeats ◽  
Clonal Complex ◽  
Fatal Outcome ◽  
Variable Number ◽  
The North ◽  
Surveillance Report ◽  
Laboratory Surveillance ◽  
Sporadic Cases ◽  
Variable Number Tandem Repeats

Two clusters of invasive meningococcal disease in the north of Italy both due to serogroup C/ST-11 clonal complex are here described. The objective of the investigation was to analyse the phenotype and the genotype of meningococci involved in the two clusters which were of national relevance due to the fatal outcome of the majority of cases (six of the total of 10 cases). All the strains were C:2a:P1.5 ST-11/ET-37 clonal complex. Two pulsed field gel electrophoresis (PFGE) and variable number tandem repeats (VNTR) profiles were identified, one for each cluster. VNTRs were different from those detected in Italy for C/ST-11 strains isolated from sporadic cases in the same period. This laboratory surveillance report highlights the importance and the crucial role of molecular characterisation to confirm the relatedness among meningococci responsible for clusters of cases.

Sign in / Sign up

Export Citation Format

Share Document