Development of a new real-time TaqMan PCR assay for quantitative analyses of Candida albicans resistance genes expression

The prevalence of CTX-M-producing members of the Enterobacteriaceae is increasing worldwide. A novel, multiplex, real-time TaqMan PCR assay to detect and type bla CTX-M genes is described which is an improvement on previously described techniques with respect to reduced assay time, elimination of the need for protracted post-PCR processing and the convenience of a single reaction vessel. Based on β-lactam antibiogram and MIC data, 478 of 1279 Enterobacteriaceae isolates from clinical blood and urine culture specimens were selected and tested for extended-spectrum β-lactamase (ESBL) production using phenotypic methods. The new TaqMan assay detected and typed bla CTX-M genes in 21 of 28 ESBL-producing isolates.

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Development of a Multitarget Real-Time TaqMan PCR Assay for Enhanced Detection of Francisella tularensis in Complex Specimens

Journal of Clinical Microbiology ◽

10.1128/jcm.41.12.5492-5499.2003 ◽

2003 ◽

Vol 41 (12) ◽

pp. 5492-5499 ◽

Cited By ~ 145

Author(s):

J. L. Versage ◽

D. D. M. Severin ◽

M. C. Chu ◽

J. M. Petersen

Keyword(s):

Real Time ◽

Francisella Tularensis ◽

Pcr Assay ◽

Taqman Pcr

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Development of a quantitative real-time TaqMan PCR assay for testing the susceptibility of feline herpesvirus-1 to antiviral compounds

Journal of Virological Methods ◽

10.1016/j.jviromet.2008.05.018 ◽

2008 ◽

Vol 152 (1-2) ◽

pp. 85-90 ◽

Cited By ~ 12

Author(s):

Islam T.M. Hussein ◽

Hugh J. Field

Keyword(s):

Real Time ◽

Pcr Assay ◽

Antiviral Compounds ◽

Feline Herpesvirus ◽

Taqman Pcr ◽

Herpesvirus 1

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Development of a Multiplex Real-Time PCR Assay for Rapid Detection of Tigecycline Resistance Gene tet(X) Variants from Bacterial, Fecal, and Environmental Samples

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.02292-19 ◽

2020 ◽

Vol 64 (4) ◽

Cited By ~ 1

Author(s):

Yulin Fu ◽

Dejun Liu ◽

Huangwei Song ◽

Zhihai Liu ◽

Haiyang Jiang ◽

...

Keyword(s):

Detection Limit ◽

Real Time ◽

Resistance Gene ◽

Resistance Genes ◽

Real Time Pcr ◽

Rapid Detection ◽

Environmental Samples ◽

Target Gene ◽

Pcr Assay ◽

High Level

ABSTRACT We developed a multiplex real-time SYBR green-based PCR assay for rapid detection of tet(X) and its variants, including tet(X1) and tet(X2) and high-level tigecycline resistance genes tet(X3), tet(X4), and tet(X5). We showed that the real-time PCR assay developed had high linearity (R2 ≥ 0.996), sensitivity (low detection limit), and specificity (only the target gene could be amplified significantly) and further evaluated it using bacterial, fecal, and environmental samples.

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Quantitative Assessment of the Tetracycline Resistance Gene Pool in Cheese Samples by Real-Time TaqMan PCR

Applied and Environmental Microbiology ◽

10.1128/aem.01994-06 ◽

2007 ◽

Vol 73 (5) ◽

pp. 1676-1677 ◽

Cited By ~ 19

Author(s):

Michele Y. Manuzon ◽

Scott E. Hanna ◽

Hongliang Luo ◽

Zhongtang Yu ◽

W. James Harper ◽

...

Keyword(s):

Real Time ◽

Gene Pool ◽

Antibiotic Resistance Genes ◽

Tetracycline Resistance ◽

Food Samples ◽

Independent Method ◽

Pcr Assay ◽

Tetracycline Resistance Gene ◽

Culture Independent ◽

Taqman Pcr

ABSTRACT A TaqMan real-time PCR assay was developed to quantify the tetS gene pool present in retail cheeses. This protocol offers a rapid, specific, sensitive, and culture-independent method for assessing antibiotic resistance genes in food samples rich in fats and proteins.

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