scholarly journals Comparison of Genotypic and Phenotypic Methods for Species-Level Identification of Clinical Isolates of Coagulase-Negative Staphylococci

2005 ◽  
Vol 43 (5) ◽  
pp. 2286-2290 ◽  
Author(s):  
E. Heikens ◽  
A. Fleer ◽  
A. Paauw ◽  
A. Florijn ◽  
A. C. Fluit
Keyword(s):  
Species Level ◽  
Clinical Isolates ◽  
Coagulase Negative Staphylococci ◽  
Phenotypic Methods ◽  
Level Identification

scholarly journals Simplified and Reliable Scheme for Species-Level Identification of Staphylococcus Clinical Isolates

2007 ◽  
Vol 45 (8) ◽  
pp. 2564-2569 ◽  
Author(s):  
N. L. P. Iorio ◽  
R. B. R. Ferreira ◽  
R. P. Schuenck ◽  
K. L. Malvar ◽  
A. P. Brilhante ◽  
...  
Keyword(s):  
Species Level ◽  
Clinical Isolates ◽  
Level Identification

scholarly journals Identification of Coryneform Bacterial Isolates by Ribosomal DNA Sequence Analysis

2000 ◽  
Vol 38 (4) ◽  
pp. 1676-1678 ◽  
Author(s):  
Yi-Wei Tang ◽  
Alexander Von Graevenitz ◽  
Michael G. Waddington ◽  
Marlene K. Hopkins ◽  
Douglas H. Smith ◽  
...  
Keyword(s):  
Clinical Intervention ◽  
Species Level ◽  
Rrna Gene ◽  
Bacterial Identification ◽  
Coryneform Bacteria ◽  
Elapsed Time ◽  
Clinically Significant ◽  
Phenotypic Methods ◽  
The 16S Rrna Gene ◽  
Level Identification

Identification of coryneform bacteria to the species level is important in certain circumstances for differentiating contamination and/or colonization from infection, which influences decisions regarding clinical intervention. However, methods currently used in clinical microbiology laboratories for the species identification of coryneform bacteria are often inadequate. We evaluated the MicroSeq 500 16S bacterial sequencing kit (Perkin-Elmer Biosystems, Foster City, Calif.), which is designed to sequence the first 527 bp of the 16S rRNA gene for bacterial identification, by using 52 coryneform gram-positive bacilli from clinical specimens isolated from January through June 1993 at the Mayo Clinic. Compared to conventional and supplemented phenotypic methods, MicroSeq provided concordant results for identification to the genus level for all isolates. At the species level, MicroSeq provided concordant results for 27 of 42 (64.3%)Corynebacterium isolates and 5 of 6 (83.3%)Corynebacterium-related isolates, respectively. Within theCorynebacterium genus, MicroSeq gave identical species-level identifications for the clinically significantCorynebacterium diphtheriae (4 of 4) andCorynebacterium jeikeium (8 of 8), but it identified only 50.0% (15 of 30) of other species (P < 0.01). Four isolates from the genera Arthrobacter,Brevibacterium, and Microbacterium, which could not be identified to the species level by conventional methods, were assigned a species-level identification by MicroSeq. The total elapsed time for running a MicroSeq identification was 15.5 to 18.5 h. These data demonstrate that the MicroSeq 500 16S bacterial sequencing kit provides a potentially powerful method for the definitive identification of clinical coryneform bacterium isolates.

scholarly journals Amplicon-Based High-Throughput Sequencing Method Capable of Species-Level Identification of Coagulase-Negative Staphylococci in Diverse Communities

2020 ◽  
Vol 8 (6) ◽  
pp. 897
Author(s):  
Emiel Van Reckem ◽  
Luc De Vuyst ◽  
Frédéric Leroy ◽  
Stefan Weckx
Keyword(s):  
High Throughput ◽  
High Throughput Sequencing ◽  
Amplicon Sequencing ◽  
Species Level ◽  
Coagulase Negative Staphylococci ◽  
Tuf Gene ◽  
Microbial Ecosystems ◽  
Gene Database ◽  
Primer Sets ◽  
Level Identification

Coagulase-negative staphylococci (CNS) make up a diverse bacterial group, appearing in a myriad of ecosystems. To unravel the composition of staphylococcal communities in these microbial ecosystems, a reliable species-level identification is crucial. The present study aimed to design a primer set for high-throughput amplicon sequencing, amplifying a region of the tuf gene with enough discriminatory power to distinguish different CNS species. Based on 2566 tuf gene sequences present in the public European Nucleotide Archive database and saved as a custom tuf gene database in-house, three different primer sets were designed, which were able to amplify a specific region of the tuf gene for 36 strains of 18 different CNS species. In silico analysis revealed that species-level identification of closely related species was only reliable if a 100% identity cut-off was applied for matches between the amplicon sequence variants and the custom tuf gene database. From the three primer sets designed, one set (Tuf387/765) outperformed the two other primer sets for studying Staphylococcus-rich microbial communities using amplicon sequencing, as it resulted in no false positives and precise species-level identification. The method developed offers interesting potential for a rapid and robust analysis of complex staphylococcal communities in a variety of microbial ecosystems.

scholarly journals Unbiased Species-Level Identification of Clinical Isolates of Coagulase-Negative Staphylococci: Does It Change the Perspective on Staphylococcus lugdunensis?

2014 ◽  
Vol 53 (1) ◽  
pp. 292-294 ◽  
Author(s):  
Wael F. Elamin ◽  
David Ball ◽  
Michael Millar
Keyword(s):  
Laser Desorption ◽  
Species Level ◽  
Laser Desorption Ionization ◽  
Coagulase Negative Staphylococci ◽  
Staphylococcus Lugdunensis ◽  
Ionization Time ◽  
Content Type ◽  
Flight Mass Spectrometry ◽  
Level Identification ◽  
Matrix Assisted Laser

Unbiased species-level identification of coagulase-negative staphylococci (CoNS) using matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) identifiedStaphylococcus lugdunensisto be a more commonly isolated CoNS in our laboratory than previously observed. It has also highlighted the possibility of vertical transmission.

scholarly journals First Report of a Gall Midge Species (Diptera: Cecidomyiidae) Associated With Pistachios

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Farshid O Sirjani ◽  
Edwin E Lewis
Keyword(s):  
Gall Midge ◽  
Species Level ◽  
Plant Tissues ◽  
First Report ◽  
Branch Dieback ◽  
First Time ◽  
Level Identification

Abstract A new dipterous pest is reported, for the first time, on commercial pistachios from Sirjan, Kerman province, Iran. The genus of the insect was determined to be Resseliella Seitner (Diptera: Cecidomyiidae). Adults are light brown to brown in color and 0.8–1.5 mm in length with females, generally, slightly larger than males. Females have an elongated ovipositor, which is characteristic of the genus. Larvae are orange in color, 2–3 mm in length in the later instars, feed under bark without inducing galls, and cause branch dieback on trees of various ages. Brown to black discolorations are observed on plant tissues under bark where the larvae feed. Infestations observed on current and the previous—year’s growths, ranged from 0.5 to 1.2 cm in diameter, and all located in outer branches. Dry leaves and fruit clusters on infested branches remain attached, which may be used to recognize infestation by the gall midge. Dark-colored, sunken spots with splits on the bark located at the base of the wilted sections of the shoots also are symptoms of Resseliella sp. larval activity. Species-level identification of the gall midge is currently underway.

scholarly journals Speciation and Antibiotic Susceptibilities of Coagulase Negative Staphylococci Isolated from Ocular Infections

Antibiotics ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 721
Author(s):  
John E. Romanowski ◽  
Shannon V. Nayyar ◽  
Eric G. Romanowski ◽  
Vishal Jhanji ◽  
Robert M. Q. Shanks ◽  
...  
Keyword(s):  
Empiric Therapy ◽  
Species Level ◽  
Opportunistic Pathogens ◽  
Coagulase Negative Staphylococci ◽  
Biochemical System ◽  
Ocular Infections ◽  
Antibiotic Susceptibilities ◽  
System 2 ◽  
Intravitreal Treatment ◽  
Susceptibility Profiles

Coagulase-negative staphylococci (CoNS) are frequently occurring ocular opportunistic pathogens that are not easily identifiable to the species level. The goal of this study was to speciate CoNS and document antibiotic susceptibilities from cases of endophthalmitis (n = 50), keratitis (n = 50), and conjunctivitis/blepharitis (n = 50) for empiric therapy. All 150 isolates of CoNS were speciated using (1) API Staph (biochemical system), (2) Biolog GEN III Microplates (phenotypic substrate system), and (3) DNA sequencing of the sodA gene. Disk diffusion antibiotic susceptibilities for topical and intravitreal treatment were determined based on serum standards. CoNS identification to the species level by all three methods indicated that S. epidermidis was the predominant species of CoNS isolated from cases of endophthalmitis (84–90%), keratitis (80–86%), and conjunctivitis/blepharitis (62–68%). Identifications indicated different distributions of CoNS species among endophthalmitis (6), keratitis (10), and conjunctivitis/blepharitis (13). Antibiotic susceptibility profiles support empiric treatment of endophthalmitis with vancomycin, and keratitis treatment with cefazolin or vancomycin. There was no clear antibiotic choice for conjunctivitis/blepharitis. S. epidermidis was the most frequently found CoNS ocular pathogen, and infection by other CoNS appears to be less specific and random. Antibiotic resistance does not appear to be a serious problem associated with CoNS.

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