scholarly journals The Structure of Sindbis Virus Produced from Vertebrate and Invertebrate Hosts as Determined by Small-Angle Neutron Scattering

2010 ◽  
Vol 84 (10) ◽  
pp. 5270-5276 ◽  
Author(s):  
Lilin He ◽  
Amanda Piper ◽  
Flora Meilleur ◽  
Dean A. A. Myles ◽  
Raquel Hernandez ◽  
...  
Keyword(s):  
Neutron Scattering ◽  
Small Angle ◽  
Host Species ◽  
Mammalian Cells ◽  
Small Angle Neutron Scattering ◽  
Sindbis Virus ◽  
Insect Cells ◽  
Lipid Membrane ◽  
Scattering Data ◽  
The Impact

ABSTRACT The complex natural cycle of vectored viruses that transition between host species, such as between insects and mammals, makes understanding the full life cycle of the virus an incredibly complex problem. Sindbis virus, an arbovirus and prototypic alphavirus having an inner protein shell and an outer glycoprotein coat separated by a lipid membrane, is one example of a vectored virus that transitions between vertebrate and insect hosts. While evidence of host-specific differences in Sindbis virus has been observed, no work has been performed to characterize the impact of the host species on the structure of the virus. Here, we report the first study of the structural differences between Sindbis viruses grown in mammalian and insect cells, which were determined by small-angle neutron scattering (SANS), a nondestructive technique that did not decrease the infectivity of the Sindbis virus particles studied. The scattering data and modeling showed that, while the radial position of the lipid bilayer did not change significantly, it was possible to conclude that it did have significantly more cholesterol when the virus was grown in mammalian cells. Additionally, the outer protein coat was found to be more extended in the mammalian Sindbis virus. The SANS data also demonstrated that the RNA and nucleocapsid protein share a closer interaction in the mammalian-cell-grown virus than in the virus from insect cells.

In situcharacterization of β′′ precipitation in an Al–Mg–Si alloy by anisotropic small-angle neutron scattering on a single crystal

2015 ◽  
Vol 48 (2) ◽  
pp. 455-463 ◽  
Author(s):  
Cynthia Sin Ting Chang ◽  
Frédéric De Geuser ◽  
John Banhart
Keyword(s):  
Neutron Scattering ◽  
Single Crystal ◽  
Small Angle ◽  
Small Angle Neutron Scattering ◽  
Scattering Data ◽  
Artificial Ageing ◽  
Number Density ◽  
Transmission Electron ◽  
Repetition Time ◽  
The Impact

A single crystal of an Al–Mg–Si alloy (Mg: 0.43 wt%, Si: 0.47 wt%) was aged at 453 K while small-angle neutron scattering experiments were carried outin situ. The scattering data recorded on a two-dimensional detector show the symmetry typical for needle-shaped β′′ precipitates oriented in the three [100] directions of the aluminium lattice and allow the calculation of the length, diameter and number density of the precipitates assuming cylindrical scattering objects of equal size and composition. The repetition time of the experiments was ∼12 min. The values obtained for the three quantities agree well with values measured by transmission electron microscopy (TEM) on a similar alloy. The impact of one week of natural ageing before artificial ageing on the evolution of the size and number density of precipitates is found to be pronounced, as expected from published TEM data.

scholarly journals Probing the Complex Loading Dependent Structural Changes in Ultra-High Drug Loaded Polymer Micelles by Small-Angle Neutron Scattering

2019 ◽  
Author(s):  
Benedikt Sochor ◽  
Özgür Düdückcü ◽  
Michael M Lübtow ◽  
Bernhard Schummer ◽  
Sebastian Jaksch ◽  
...  
Keyword(s):  
Neutron Scattering ◽  
Small Angle ◽  
Small Angle Neutron Scattering ◽  
Drug Loading ◽  
Scattering Data ◽  
Resonance Spectroscopy ◽  
Polymer Micelle ◽  
Polymer Micelles ◽  
The Core ◽  
The Impact

Drug loaded polymer micelles or nanoparticles are being continuously explored in the fields of drug delivery and nanomedicine. Commonly, a simple core-shell structure is assumed, in which the core incorporates the drug and the corona provides steric shielding, colloidal stability, and prevents protein adsorption. Recently, the interactions of the dissolved drug with the micellar corona have received increasing attention. Here, using small-angle neutron scattering, we provide an in-depth study of the differences in polymer micelle morphology of a small selection of structurally closely related polymer micelles at different loadings with the model compound curcumin. This work supports a previous study using solid state nuclear magnetic resonance spectroscopy and we confirm that the drug resides predominantly in the core of the micelle at low drug loading. As the drug loading increases, neutron scattering data suggests that an inner shell is formed, which we interpret as the corona also starting to incorporate the drug, whereas the outer shell mainly contains water and the polymer. The presented data clearly shows that a better understanding of the inner morphology and the impact of the hydrophilic block can be important parameters for improved drug loading in polymer micelles as well as provide insights into structure-property relationships.<br>

scholarly journals Probing the Complex Loading Dependent Structural Changes in Ultra-High Drug Loaded Polymer Micelles by Small-Angle Neutron Scattering

2019 ◽  
Author(s):  
Benedikt Sochor ◽  
Özgür Düdückcü ◽  
Michael M Lübtow ◽  
Bernhard Schummer ◽  
Sebastian Jaksch ◽  
...  
Keyword(s):  
Neutron Scattering ◽  
Small Angle ◽  
Small Angle Neutron Scattering ◽  
Drug Loading ◽  
Scattering Data ◽  
Resonance Spectroscopy ◽  
Polymer Micelle ◽  
Polymer Micelles ◽  
The Core ◽  
The Impact

Drug loaded polymer micelles or nanoparticles are being continuously explored in the fields of drug delivery and nanomedicine. Commonly, a simple core-shell structure is assumed, in which the core incorporates the drug and the corona provides steric shielding, colloidal stability, and prevents protein adsorption. Recently, the interactions of the dissolved drug with the micellar corona have received increasing attention. Here, using small-angle neutron scattering, we provide an in-depth study of the differences in polymer micelle morphology of a small selection of structurally closely related polymer micelles at different loadings with the model compound curcumin. This work supports a previous study using solid state nuclear magnetic resonance spectroscopy and we confirm that the drug resides predominantly in the core of the micelle at low drug loading. As the drug loading increases, neutron scattering data suggests that an inner shell is formed, which we interpret as the corona also starting to incorporate the drug, whereas the outer shell mainly contains water and the polymer. The presented data clearly shows that a better understanding of the inner morphology and the impact of the hydrophilic block can be important parameters for improved drug loading in polymer micelles as well as provide insights into structure-property relationships.<br>

Autocorrelation function of the spin misalignment in magnetic small-angle neutron scattering: application to nanocrystalline metals

2013 ◽  
Vol 46 (3) ◽  
pp. 788-790 ◽  
Author(s):  
Andreas Michels ◽  
Jens-Peter Bick
Keyword(s):  
Neutron Scattering ◽  
Autocorrelation Function ◽  
Small Angle ◽  
Small Angle Neutron Scattering ◽  
Anisotropy Field ◽  
Real Space ◽  
Scattering Data ◽  
Stiffness Constant ◽  
The Mean ◽  
Cobalt And Nickel

Real-space magnetic small-angle neutron scattering data from nanocrystalline cobalt and nickel have been analysed in terms of a recently developed micromagnetic theory for the autocorrelation function of the spin misalignment [Michels (2010).Phys. Rev. B,82, 024433]. The approach provides information on the exchange-stiffness constant and on the mean magnetic `anisotropy-field' radius.

Characterization of alumina powder using multiple small-angle neutron scattering. I. Theory

1985 ◽  
Vol 18 (6) ◽  
pp. 467-472 ◽  
Author(s):  
N. F. Berk ◽  
K. A. Hardman-Rhyne
Keyword(s):  
Particle Size ◽  
Phase Shift ◽  
Neutron Scattering ◽  
Multiple Scattering ◽  
Small Angle ◽  
Small Angle Neutron Scattering ◽  
Volume Fraction ◽  
Scattering Data ◽  
Alumina Powder ◽  
Beam Broadening

Microstructural parameters of high-purity alumina powder are determined quantitatively throughout the bulk of the material using small-angle neutron scattering techniques. A unified theoretical and experimental approach for analyzing multiple scattering data is developed to obtain values for particle size, volume fraction and surface area. It is shown how particle size and volume fraction can be measured in a practical way from SANS data totally dominated by incoherent multiple scattering (`beam broadening'). The general phase-shift dependence of single-particle scattering is incorporated into the multiple scattering formalism, and it is also shown that the diffractive limit (small phase shift) applies even for phase shifts as large as unity (particle radii of order 1 μm). The stability of the Porod law against multiple scattering and the phase-shift scale are described, a useful empirical formula for analysis of beam broadening data is exhibited, and the applicability of the formulations to polydispersed systems is discussed.

Structure of Silicon-Substituted Polytricyclononene Films: Small-Angle Neutron Scattering Data

2018 ◽  
Vol 60 (10) ◽  
pp. 2097-2102
Author(s):  
V. T. Lebedev ◽  
N. P. Yevlampieva ◽  
M. V. Bermeshev ◽  
A. A. Szhogina
Keyword(s):  
Neutron Scattering ◽  
Small Angle ◽  
Small Angle Neutron Scattering ◽  
Scattering Data

Small Angle Neutron Scatitering Study of Sol-Gel Glasses

1989 ◽  
Vol 166 ◽  
Author(s):  
P. Wiltzius ◽  
S. B. Dierker
Keyword(s):  
Neutron Scattering ◽  
Small Angle ◽  
Structure Factor ◽  
Small Angle Neutron Scattering ◽  
Sol Gel ◽  
Scattering Data ◽  
Length Scales ◽  
Internal Interface ◽  
Porous Glasses ◽  
Gel Glasses

ABSTRACTWe present small angle neutron scattering data of porous glasses. Analysis of the structure factor shows that the morphology on length scales between 30 A and 800 A depends on fabrication procedures. Fast gelation leads to a clumpy glass, whereas slow gelation produces a random smooth internal interface.

scholarly journals Revealing inner structure of the polycarbosilane dendrimers from small-angle neutron scattering data

2008 ◽  
Vol 129 ◽  
pp. 012041 ◽  
Author(s):  
A V Rogachev ◽  
A Yu Cherny ◽  
A N Ozerin ◽  
A M Muzafarov ◽  
E Atatarinova ◽  
...  
Keyword(s):  
Neutron Scattering ◽  
Small Angle ◽  
Small Angle Neutron Scattering ◽  
Scattering Data

scholarly journals The Small-Angle Neutron Scattering Data Analysis of the Phospholipid Transport Nanosystem Structure

2018 ◽  
Vol 1023 ◽  
pp. 012017 ◽  
Author(s):  
E.V. Zemlyanaya ◽  
M.A. Kiselev ◽  
E.I. Zhabitskaya ◽  
V.L. Aksenov ◽  
O.M. Ipatova ◽  
...  
Keyword(s):  
Data Analysis ◽  
Neutron Scattering ◽  
Small Angle ◽  
Small Angle Neutron Scattering ◽  
Scattering Data ◽  
Phospholipid Transport
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