Noninvasive Near-Infrared Light Triggers Remote Activation of Thermo-Responsive TRPV1 Channels in Neurons Based on Biodegradable/Photothermal Polymer Micelles

In this study, we developed a novel biodegradable/photothermal polymer micelle-based remote-activation method for a temperature-sensitive ion channel, namely transient receptor potential cation channel subfamily V member 1 (TRPV1). Biodegradable/photothermal polymer...

Construction of gambogic acid HPMA Copolymer Coupling drug system and study on anti-tumor activity

Purpose: An active-passive dual-targeting gambogic acid HPMA Copolymer Coupling drug system with high efficiency, low toxicity and high selectivity was constructed. Methods: The gambogic acid HPMA copolymer coupling drug system was constructed and its structure was characterized. The cytotoxicity of gambogic acid HPMA copolymer was detected by MTT assay. The pharmacokinetics of gambogic acid HPMA copolymer was evaluated in mice. Targetability of gambogic acid HPMA copolymer was evaluated by tissue distribution experiment. The in vitro antitumor activity of gambogic acid HPMA copolymer was evaluated by pharmacodynamics experiment in mice. Results : Two copolymers of gambogic acid HPMA were successfully prepared. The copolymers showed reduced cytotoxicity and a certain sustained release effect and targeting property. In vivo pharmacodynamic experiments also showed better anti-tumor effects than GA. Discussion: In this study, gambogic acid was combined with HPMA polymer and the targeting molecule D-galactose/folic acid to form a polymer micelle with high efficiency, low toxicity and high selectivity for active-passive dual targeting. The construction of the drug system provides new ideas for future formulation research and development.

Neutron investigation of interaction between anionic surfactant micelles and poly (ethylene glycol) polymer brush system

A polymer brush system of a neutral polymer poly (ethylene glycol) with a molecular weight of Mw = 20 kDa on silicon substrates in an aqueous medium was studied by the specular neutron reflectometry. Structural changes in the density profile of a polymer brush caused by the interaction of polymer chains with micelles of the anionic surfactant dodecylbenzenesulfonate acid were observed. The effect is shown to be related to the formation of molecular polymer-micelle associates in the bulk of the solution, which was previously studied by small-angle neutron scattering in a wide range of surfactant concentrations at various molecular weights of the polymer. The density of the dry polymer layer on the silicon substrate was additionally characterized by X-ray reflectometry and scanning atomic force microscopy.

Evaluation of the Efficacy and Performance of a New Nano-Drug Carrier in the Treatment of Recurrent Oral Ulcerper

Recurrent oral ulcer is a common oral mucosal disease. Due to its periodic and recurrent characteristics, the onset of burning pain is unbearable, which brings great inconvenience to the patient’s life and seriously affects the patient’s quality of life. There are certain limitations to conventional drug therapy. With the rapid development of nanotechnology, the obvious advantages of nanotechnology such as targeting, controlled release, biocompatibility is obviously shown. The combination of nanotechnology and medical research has led to the emergence of polymer nanoparticles and ligands. Nano-drugs for gene therapy, and many other new nano-drug carriers, polymer micelle is a new type of nano drug carrier that has appeared in recent years. It has both a hydrophilic shell and a hydrophobic core, and has a variety of excellent properties, such as higher stability in vivo and in vitro, and poorly soluble drugs. In this paper, a new method for treating recurrent oral ulcers based on a new nano-drug carrier was studied. Because of the hydrophilicity and biocompatibility of oral cell surface proteins, the research progress of nanopharmaceutical carrier in the treatment of recurrent oral ulcers is reviewed. The experimental results show that the method has good reproducibility and high efficiency in the treatment of recurrent oral ulcers. It is used to explore the application and progress of nanotechnology in the diagnosis and treatment of recurrent oral ulcers, and to provide new ideas for the clinical diagnosis and treatment of recurrent oral ulcers. This new technical method has wide practical application value.

4D imaging of soft matter in liquid water

Water is a critical component for both function and structure of soft matter and it is what bestows the adjective soft. Imaging samples in liquid state is thus paramount to gathering structural and dynamical information of any soft materials. Herein we propose the use of liquid phase electron microscopy to expand ultrastructural analysis into dynamical investigations. We imaged two soft matter examples: a polymer micelle and a protein in liquid phase using transmission electron microscopy and demonstrate that the inherent Brownian motion associated with the liquid state can be exploited to gather three-dimensional information of the materials in their natural state. We call such an approach brownian tomography (BT). We combine BT with single particle analysis (Brownian particle analysis BPA) to image protein structures with a spatial resolution close that achievable using cryogenic TEM. We show that BPA allows sub-nanometer resolution of soft materials and enables to gather information on conformational changes, hydration dynamics, and the effect of thermal fluctuations.Abstract Figure