scholarly journals Infection of Human Airway Epithelium by Human and Avian Strains of Influenza A Virus

2006 ◽  
Vol 80 (16) ◽  
pp. 8060-8068 ◽  
Author(s):  
Catherine I. Thompson ◽  
Wendy S. Barclay ◽  
Maria C. Zambon ◽  
Raymond J. Pickles
Keyword(s):  
Sialic Acid ◽  
Avian Influenza ◽  
Influenza A Virus ◽  
Airway Epithelium ◽  
Influenza A ◽  
Cell Types ◽  
Influenza Viruses ◽  
Apical Surface ◽  
Ciliated Cells ◽  
Human Viruses

ABSTRACT We describe the characterization of influenza A virus infection of an established in vitro model of human pseudostratified mucociliary airway epithelium (HAE). Sialic acid receptors for both human and avian viruses, α-2,6- and α-2,3-linked sialic acids, respectively, were detected on the HAE cell surface, and their distribution accurately reflected that in human tracheobronchial tissue. Nonciliated cells present a higher proportion of α-2,6-linked sialic acid, while ciliated cells possess both sialic acid linkages. Although we found that human influenza viruses infected both ciliated and nonciliated cell types in the first round of infection, recent human H3N2 viruses infected a higher proportion of nonciliated cells in HAE than a 1968 pandemic-era human virus, which infected proportionally more ciliated cells. In contrast, avian influenza viruses exclusively infected ciliated cells. Although a broad-range neuraminidase abolished infection of HAE by human parainfluenza virus type 3, this treatment did not significantly affect infection by influenza viruses. All human viruses replicated efficiently in HAE, leading to accumulation of nascent virus released from the apical surface between 6 and 24 h postinfection with a low multiplicity of infection. Avian influenza A viruses also infected HAE, but spread was limited compared to that of human viruses. The nonciliated cell tropism of recent human H3N2 viruses reflects a preference for the sialic acid linkages displayed on these cell types and suggests a drift in the receptor binding phenotype of the H3 hemagglutinin protein as it evolves in humans away from its avian virus precursor.

scholarly journals Modern application and prospects of the stable isotopes  method for studying avian influenza A virus transmission   in migratory birds

2019 ◽  
Vol 14 (3) ◽  
pp. 92-100
Author(s):  
O. R. Druzyaka ◽  
A. V. Druzyaka ◽  
M. A. Gulyaeva ◽  
F. Huettmann ◽  
A. M. Shestopalov
Keyword(s):  
Stable Isotopes ◽  
Avian Influenza ◽  
Influenza A Virus ◽  
Influenza A ◽  
Bird Migration ◽  
Migratory Birds ◽  
Influenza Viruses ◽  
Migration Routes ◽  
Viral Pathogen ◽  
Avian Influenza A Virus

Aim. The circulation and transmission of pathogens is a global biological phenomenon that is closely associated with bird migration. This analysis was carried out with  the aim of understanding and assessing the prospects of using the stable isotope  method to study the circulation and transmission of the avian influenza A virus via  migratory birds. Discussion. Insufficient data on the distances of migration of infected birds and their  interpopulational relationships leaves open the question of the transmission of highly pathogenic influenza viruses (HSV) in the wild bird population. A deeper study of  the role of migrations in the spread of HSV may possibly allow the more effective  investigation of the transmission of the viral pathogen between individuals at migration stopover sites and the clarification of global migration routes. New methodological approaches are providing a more complete picture of the geography and phenology of migrations, as well as of the consequences of migratory behavior for species biology. The study of the quantitative component of migratory flows based on  the analysis of the content of stable isotopes (SIMS) in bird tissues seems very promising. This method is being applied to the solution of various environmental issues,  including the study of animal migrations.   Conclusion. Based on data from the scientific literature, it is shown that SIMS is  promising for the clarification of bird migration routes and the quantification of their  intensity. The resolving power of the method is sufficient to determine the migration  pathways of carriers of viral pathogens on the scale of zoogeographic subdomains  and in even further detail. However, to date, there have been few such studies: in  Russia they have not been conducted at all. The increased use of the SIMS methodology may possibly reveal new ways in which viral infections are spread via birds.  

scholarly journals Sialic Acid Species as a Determinant of the Host Range of Influenza A Viruses

2000 ◽  
Vol 74 (24) ◽  
pp. 11825-11831 ◽  
Author(s):  
Yasuo Suzuki ◽  
Toshihiro Ito ◽  
Takashi Suzuki ◽  
Robert E. Holland ◽  
Thomas M. Chambers ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Viral Replication ◽  
Influenza A ◽  
Lectin Binding ◽  
Influenza Viruses ◽  
Influenza A Viruses ◽  
Acetylneuraminic Acid ◽  
Human Viruses ◽  
Immunohistochemical Analyses

ABSTRACT The distribution of sialic acid (SA) species varies among animal species, but the biological role of this variation is largely unknown. Influenza viruses differ in their ability to recognize SA-galactose (Gal) linkages, depending on the animal hosts from which they are isolated. For example, human viruses preferentially recognize SA linked to Gal by the α2,6(SAα2,6Gal) linkage, while equine viruses favor SAα2,3Gal. However, whether a difference in relative abundance of specific SA species (N-acetylneuraminic acid [NeuAc] andN-glycolylneuraminic acid [NeuGc]) among different animals affects the replicative potential of influenza viruses is uncertain. We therefore examined the requirement for the hemagglutinin (HA) for support of viral replication in horses, using viruses whose HAs differ in receptor specificity. A virus with an HA recognizing NeuAcα2,6Gal but not NeuAcα2,3Gal or NeuGcα2,3Gal failed to replicate in horses, while one with an HA recognizing the NeuGcα2,3Gal moiety replicated in horses. Furthermore, biochemical and immunohistochemical analyses and a lectin-binding assay demonstrated the abundance of the NeuGcα2,3Gal moiety in epithelial cells of horse trachea, indicating that recognition of this moiety is critical for viral replication in horses. Thus, these results provide evidence of a biological effect of different SA species in different animals.

scholarly journals In vitro demonstration of neural transmission of avian influenza A virus

2005 ◽  
Vol 86 (4) ◽  
pp. 1131-1139 ◽  
Author(s):  
Kazuya Matsuda ◽  
Takuma Shibata ◽  
Yoshihiro Sakoda ◽  
Hiroshi Kida ◽  
Takashi Kimura ◽  
...  
Keyword(s):  
Avian Influenza ◽  
Influenza A Virus ◽  
Intermediate Filaments ◽  
Influenza A ◽  
Pseudorabies Virus ◽  
Influenza Viruses ◽  
Avian Influenza A Virus ◽  
Neural Transmission ◽  
The Central Nervous System

Neural involvement following infections of influenza viruses can be serious. The neural transport of influenza viruses from the periphery to the central nervous system has been indicated by using mouse models. However, no direct evidence for neuronal infection has been obtained in vitro and the mechanisms of neural transmission of influenza viruses have not been reported. In this study, the transneural transmission of a neurotropic influenza A virus was examined using compartmentalized cultures of neurons from mouse dorsal root ganglia, and the results were compared with those obtained using the pseudorabies virus, a virus with well-established neurotransmission. Both viruses reached the cell bodies of the neurons via the axons. This is the first report on axonal transport of influenza A virus in vitro. In addition, the role of the cytoskeleton (microtubules, microfilaments and intermediate filaments) in the neural transmission of influenza virus was investigated by conducting cytoskeletal perturbation experiments. The results indicated that the transport of avian influenza A virus in the neurons was independent of microtubule integrity but was dependent on the integrity of intermediate filaments, whereas pseudorabies virus needed both for neural spread.

scholarly journals Laboratory preparedness in EU/EEA countries for detection of novel avian influenza A(H7N9) virus, May 2013

2014 ◽  
Vol 19 (4) ◽  
Author(s):  
E Broberg ◽  
D Pereyaslov ◽  
M Struelens ◽  
D Palm ◽  
A Meijer ◽  
...  
Keyword(s):  
Avian Influenza ◽  
Real Time ◽  
Influenza A Virus ◽  
Influenza A ◽  
Virus Detection ◽  
Influenza Viruses ◽  
World Health ◽  
Reference Laboratory ◽  
The Novel ◽  
Rt Pcr

Following human infections with novel avian influenza A(H7N9) viruses in China, the European Centre for Disease Prevention and Control, the World Health Organization (WHO) Regional Office for Europe and the European Reference Laboratory Network for Human Influenza (ERLI-Net) rapidly posted relevant information, including real-time RT-PCR protocols. An influenza RNA sequence-based computational assessment of detection capabilities for this virus was conducted in 32 national influenza reference laboratories in 29 countries, mostly WHO National Influenza Centres participating in the WHO Global Influenza Surveillance and Response System (GISRS). Twenty-seven countries considered their generic influenza A virus detection assay to be appropriate for the novel A(H7N9) viruses. Twenty-two countries reported having containment facilities suitable for its isolation and propagation. Laboratories in 27 countries had applied specific H7 real-time RT-PCR assays and 20 countries had N9 assays in place. Positive control virus RNA was provided by the WHO Collaborating Centre in London to 34 laboratories in 22 countries to allow evaluation of their assays. Performance of the generic influenza A virus detection and H7 and N9 subtyping assays was good in 24 laboratories in 19 countries. The survey showed that ERLI-Net laboratories had rapidly developed and verified good capability to detect the novel A(H7N9) influenza viruses.

scholarly journals Comparison of serum treatments to remove nonspecific inhibitors from chicken sera for the hemagglutination inhibition test with inactivated H5N1 and H9N2 avian Influenza A virus subtypes

2012 ◽  
Vol 24 (5) ◽  
pp. 954-958 ◽  
Author(s):  
Hye-Ryoung Kim ◽  
Kyoung-Ki Lee ◽  
Yong-Kuk Kwon ◽  
Min-Su Kang ◽  
Oun-Kyung Moon ◽  
...  
Keyword(s):  
Avian Influenza ◽  
Influenza A Virus ◽  
Hemagglutination Inhibition ◽  
Influenza A ◽  
Influenza Viruses ◽  
Avian Influenza Viruses ◽  
Chicken Serum ◽  
Hemagglutination Inhibition Test ◽  
Avian Influenza A Virus ◽  
Cell Adsorption

The hemagglutination inhibition (HI) assay is the standard diagnostic test for detection of antibodies to avian influenza viruses. It is well known that chicken serum does not require additional serum pretreatment to remove nonspecific inhibitors (NSIs). However, NSIs were recognized in certain Korean local breeds. In the present study, various treatments were compared to remove such NSIs. Heat treatment, red blood cell adsorption, and kaolin treatment did not remove NSIs effectively, and treatment with periodate only partly eliminated the NSIs. Receptor destroying enzyme (RDE) treatment appeared to effectively remove NSIs from chicken sera, regardless of breeds. It is proposed that RDE treatment should be included in the HI tests for serological diagnosis of avian Influenza A virus.

scholarly journals Substrate Binding by the Second Sialic Acid-Binding Site of Influenza A Virus N1 Neuraminidase Contributes to Enzymatic Activity

2018 ◽  
Vol 92 (20) ◽  
Author(s):  
Wenjuan Du ◽  
Meiling Dai ◽  
Zeshi Li ◽  
Geert-Jan Boons ◽  
Ben Peeters ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Enzymatic Activity ◽  
Binding Site ◽  
Influenza A Virus ◽  
Influenza A ◽  
Substrate Binding ◽  
Host Tropism ◽  
Contact Residues ◽  
Human Viruses

ABSTRACTThe influenza A virus (IAV) neuraminidase (NA) protein plays an essential role in the release of virus particles from cells and decoy receptors. The NA enzymatic activity presumably needs to match the activity of the IAV hemagglutinin (HA) attachment protein and the host sialic acid (SIA) receptor repertoire. We analyzed the enzymatic activities of N1 NA proteins derived from avian (H5N1) and human (H1N1) IAVs and analyzed the role of the second SIA-binding site, located adjacent to the conserved catalytic site, therein. SIA contact residues in the second SIA-binding site of NA are highly conserved in avian, but not human, IAVs. All N1 proteins preferred cleaving α2,3- over α2,6-linked SIAs even when their corresponding HA proteins displayed a strict preference for α2,6-linked SIAs, indicating that the specificity of the NA protein does not need to fully match that of the corresponding HA protein. NA activity was affected by substitutions in the second SIA-binding site that are observed in avian and human IAVs, at least when multivalent rather than monovalent substrates were used. These mutations included both SIA contact residues and residues that do not directly interact with SIA in all three loops of the second SIA-binding site. Substrate binding via the second SIA-binding site enhanced the catalytic activity of N1. Mutation of the second SIA-binding site was also shown to affect virus replicationin vitro. Our results indicate an important role for the N1 second SIA-binding site in binding to and cleavage of multivalent substrates.IMPORTANCEAvian and human influenza A viruses (IAVs) preferentially bind α2,3- and α2,6-linked sialic acids (SIAs), respectively. A functional balance between the hemagglutinin (HA) attachment and neuraminidase (NA) proteins is thought to be important for host tropism. What this balance entails at the molecular level is, however, not well understood. We now show that N1 proteins of both avian and human viruses prefer cleaving avian- over human-type receptors although human viruses were relatively better in cleavage of the human-type receptors. In addition, we show that substitutions at different positions in the second SIA-binding site found in NA proteins of human IAVs have a profound effect on binding and cleavage of multivalent, but not monovalent, receptors and affect virus replication. Our results indicate that the HA-NA balance can be tuned via modification of substrate binding via this site and suggest an important role of the second SIA-binding site in host tropism.

scholarly journals ANALYSIS OF MIGRATION OF AQUATIC AND SEMIAQUATIC BIRDS ON THE TERRITORY OF THE REPUBLIC OF DAGESTAN AND JUSTIFICATION OF THE CHOICE OF KEY POINTS OF MONITORING OF INFLUENZA A VIRUS

2019 ◽  
Vol 14 (1) ◽  
pp. 137-149
Author(s):  
A. Yu. Alekseev ◽  
T. A. Murashkina ◽  
J. M. Jamalutdinov ◽  
S. S. Abdullaev ◽  
K. A. Akhmedrabadanov ◽  
...  
Keyword(s):  
Avian Influenza ◽  
Influenza A Virus ◽  
Influenza A ◽  
Influenza Infection ◽  
Influenza Viruses ◽  
Migration Routes ◽  
Scientific Publications ◽  
Bird Populations ◽  
Large Populations ◽  
The Republic

Aim. The aim of the work is to carry out an analysis of the wetlands of the Republic of Dagestan in order to justify the selection of the collecting sites for material from migratory aquatic and semi aquatic birds in order to monitor the influenza A virus.Material and methods. Studying scientific publications of different years and available information on the wetlands of the Caspian Dagestan allowed establishing the areas of concentration of aquatic and semiaquatic birds where effective sampling for avian influenza is possible.Results. The spread of avian influenza viruses in nature is inextricably linked with migration of birds. Due to the presence of a large number of reservoirs, the western part of the Caspian region brings together large populations of wild waterbirds from various places within their migration routes. Mass accumulation encourages the interaction of birds of different species and populations, which in turn creates favorable conditions for the spread of various viral diseases.Conclusion. For an integrated assessment of the state of aquatic and semiaquatic bird populations, as well as monitoring the avian influenza infection rates, it is proposed to consider as model areas the wetlands of the Lake Aji (Papas), Lake Yuzhny Agrakhan, Agrakhansky Gulf, the Terek River delta and the Achikolsky systems of lake.

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