scholarly journals Influence of pr-M Cleavage on the Heterogeneity of Extracellular Dengue Virus Particles

2010 ◽  
Vol 84 (16) ◽  
pp. 8353-8358 ◽  
Author(s):  
Jiraphan Junjhon ◽  
Thomas J. Edwards ◽  
Utaiwan Utaipat ◽  
Valorie D. Bowman ◽  
Heather A. Holdaway ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Virus Replication ◽  
Envelope Glycoprotein ◽  
Cryoelectron Microscopy ◽  
Virus Maturation ◽  
Virus Particles ◽  
Viral Particles ◽  
Incomplete Cleavage

ABSTRACT During dengue virus replication, an incomplete cleavage of the envelope glycoprotein prM, generates a mixture of mature (prM-less) and prM-containing, immature extracellular particles. In this study, sequential immunoprecipitation and cryoelectron microscopy revealed a third type of extracellular particles, the partially mature particles, as the major prM-containing particles in a dengue serotype 2 virus. Changes in the proportion of viral particles in the pr-M junction mutants exhibiting altered levels of prM cleavage suggest that the partially mature particles may represent an intermediate subpopulation in the virus maturation pathway. These findings are consistent with a model suggesting the progressive mode of prM cleavage.

scholarly journals Functional importance of dengue virus maturation: infectious properties of immature virions

2008 ◽  
Vol 89 (12) ◽  
pp. 3047-3051 ◽  
Author(s):  
Izabela A. Zybert ◽  
Heidi van der Ende-Metselaar ◽  
Jan Wilschut ◽  
Jolanda M. Smit
Keyword(s):  
Dengue Virus ◽  
Surface Protein ◽  
General Assumption ◽  
Wild Type Virus ◽  
Virus Maturation ◽  
Virus Particles ◽  
Lovo Cells ◽  
Particle Ratio ◽  
Infected Cells ◽  
Infectious Titre

Prior to the release of flavivirus particles from infected cells, the viral surface protein prM is cleaved to M by the cellular enzyme furin. For dengue virus (DENV), this maturation process appears to be very inefficient since a high proportion of progeny virions contain uncleaved prM. Furthermore, it has been reported that prM-containing DENV particles are infectious. These observations contradict the general assumption that prM processing is required to render virus particles infectious. Therefore, in this study, we reinvestigated the infectious properties of immature DENV virions. DENV particles were produced in furin-deficient LoVo cells. We observed that DENV-infected LoVo cells secrete high numbers of prM-containing particles. Subsequent analysis of the infectious titre revealed that immature particles lack the ability to infect cells, the infectious unit to particle ratio being 10 000-fold reduced compared with that of wild-type virus. Our results indicate that cleavage of prM to M is required for DENV infectivity.

scholarly journals Differential Localization of Structural and Non-Structural Proteins during the Bluetongue Virus Replication Cycle

Viruses ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 343 ◽  
Author(s):  
Bjorn-Patrick Mohl ◽  
Adeline Kerviel ◽  
Thomas Labadie ◽  
Eiko Matsuo ◽  
Polly Roy
Keyword(s):  
Virus Replication ◽  
Bluetongue Virus ◽  
Structured Illumination ◽  
Structural Protein ◽  
Structured Illumination Microscopy ◽  
Virus Maturation ◽  
Virus Particles ◽  
Active Core ◽  
Infected Cells ◽  
Virus Replication Cycle

Members of the Reoviridae family assemble virus factories within the cytoplasm of infected cells to replicate and assemble virus particles. Bluetongue virus (BTV) forms virus inclusion bodies (VIBs) that are aggregates of viral RNA, certain viral proteins, and host factors, and have been shown to be sites of the initial assembly of transcriptionally active virus-like particles. This study sought to characterize the formation, composition, and ultrastructure of VIBs, particularly in relation to virus replication. In this study we have utilized various microscopic techniques, including structured illumination microscopy, and virological assays to show for the first time that the outer capsid protein VP5, which is essential for virus maturation, is also associated with VIBs. The addition of VP5 to assembled virus cores exiting VIBs is required to arrest transcriptionally active core particles, facilitating virus maturation. Furthermore, we observed a time-dependent association of the glycosylated non-structural protein 3 (NS3) with VIBs, and report on the importance of the two polybasic motifs within NS3 that facilitate virus trafficking and egress from infected cells at the plasma membrane. Thus, the presence of VP5 and the dynamic nature of NS3 association with VIBs that we report here provide novel insight into these previously less well-characterized processes.

scholarly journals Antiviral Role of Phenolic Compounds against Dengue Virus: A Review

Biomolecules ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 11
Author(s):  
Vanessa Loaiza-Cano ◽  
Laura Milena Monsalve-Escudero ◽  
Carlos da Silva Maia Bezerra Filho ◽  
Marlen Martinez-Gutierrez ◽  
Damião Pergentino de Sousa
Keyword(s):  
Phenolic Compounds ◽  
Dengue Virus ◽  
Biological Activities ◽  
Antiviral Effect ◽  
Health Concern ◽  
Host Proteins ◽  
Viral Particles ◽  
Antiviral Role ◽  
Productive Replication

Phenolic compounds have been related to multiple biological activities, and the antiviral effect of these compounds has been demonstrated in several viral models of public health concern. In this review, we show the antiviral role of phenolic compounds against dengue virus (DENV), the most widespread arbovirus globally that, after its re-emergence, has caused multiple epidemic outbreaks, especially in the last two years. Twenty phenolic compounds with anti-DENV activity are discussed, including the multiple mechanisms of action, such as those directed against viral particles or viral proteins, host proteins or pathways related to the productive replication viral cycle and the spread of the infection.

scholarly journals Extract of Scutellaria baicalensis inhibits dengue virus replication

2013 ◽  
Vol 13 (1) ◽  
Author(s):  
Keivan Zandi ◽  
Tong-Hye Lim ◽  
Nor-Aziyah Rahim ◽  
Meng-Hooi Shu ◽  
Boon-Teong Teoh ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Virus Replication ◽  
Scutellaria Baicalensis

scholarly journals Fusion of Protegrin-1 and Plectasin to MAP30 Shows Significant Inhibition Activity against Dengue Virus Replication

PLoS ONE ◽  
2014 ◽  
Vol 9 (4) ◽  
pp. e94561 ◽  
Author(s):  
Hussin A. Rothan ◽  
Hirbod Bahrani ◽  
Zulqarnain Mohamed ◽  
Noorsaadah Abd Rahman ◽  
Rohana Yusof
Keyword(s):  
Dengue Virus ◽  
Virus Replication ◽  
Significant Inhibition ◽  
Inhibition Activity

scholarly journals Characterization of the Early Events in Dengue Virus Cell Entry by Biochemical Assays and Single-Virus Tracking

2007 ◽  
Vol 81 (21) ◽  
pp. 12019-12028 ◽  
Author(s):  
Hilde M. van der Schaar ◽  
Michael J. Rust ◽  
Barry-Lee Waarts ◽  
Heidi van der Ende-Metselaar ◽  
Richard J. Kuhn ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Dengue Virus ◽  
Membrane Fusion ◽  
High Ratio ◽  
Cell Entry ◽  
Viral Membrane ◽  
Virus Particles ◽  
Biochemical Assays ◽  
Particle Ratio ◽  
Infectious Unit

ABSTRACT In this study, we investigated the cell entry characteristics of dengue virus (DENV) type 2 strain S1 on mosquito, BHK-15, and BS-C-1 cells. The concentration of virus particles measured by biochemical assays was found to be substantially higher than the number of infectious particles determined by infectivity assays, leading to an infectious unit-to-particle ratio of approximately 1:2,600 to 1:72,000, depending on the specific assays used. In order to explain this high ratio, we investigated the receptor binding and membrane fusion characteristics of single DENV particles in living cells using real-time fluorescence microscopy. For this purpose, DENV was labeled with the lipophilic fluorescent probe DiD (1,1′-dioctadecyl-3,3,3′,3′-tetramethylindodicarbocyanine, 4-chlorobenzenesulfonate salt). The surface density of the DiD dye in the viral membrane was sufficiently high to largely quench the fluorescence intensity but still allowed clear detection of single virus particles. Fusion of the viral membrane with the cell membrane was evident as fluorescence dequenching. It was observed that DENV binds very inefficiently to the cells used, explaining at least in part the high infectious unit-to-particle ratio. The particles that did bind to the cells showed different types of transport behavior leading to membrane fusion in both the periphery and perinuclear regions of the cell. Membrane fusion was observed in 1 out of 6 bound virus particles, indicating that a substantial fraction of the virus has the capacity to fuse. DiD dequenching was completely inhibited by ammonium chloride, demonstrating that fusion occurs exclusively from within acidic endosomes.

Tenovin-1 inhibited dengue virus replication through SIRT2

2021 ◽  
pp. 174264
Author(s):  
Yihong Wan ◽  
Wenyu Wu ◽  
Jiawen Zhang ◽  
Liren Li ◽  
Yuanda Wan ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Virus Replication

scholarly journals Different Cross-Reactivities of IgM Responses in Dengue, Zika and Tick-Borne Encephalitis Virus Infections

Viruses ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 596
Author(s):  
Karin Stiasny ◽  
Stefan Malafa ◽  
Stephan W. Aberle ◽  
Iris Medits ◽  
Georgios Tsouchnikas ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Yellow Fever ◽  
Vaccination Coverage ◽  
Virus Infections ◽  
Recent Infection ◽  
Specific Diagnosis ◽  
Virus Particles ◽  
Tick Borne Encephalitis ◽  
Structural Peculiarities ◽  
Tick Borne Encephalitis Virus

Flaviviruses circulate worldwide and cause a number of medically relevant human diseases, such as dengue, Zika, yellow fever, and tick-borne encephalitis (TBE). Serology plays an important role in the diagnosis of flavivirus infections, but can be impeded by antigenic cross-reactivities among flaviviruses. Therefore, serological diagnosis of a recent infection can be insufficiently specific, especially in areas where flaviviruses co-circulate and/or vaccination coverage against certain flaviviruses is high. In this study, we developed a new IgM assay format, which is well suited for the specific diagnosis of TBE, Zika and dengue virus infections. In the case of TBE and Zika, the IgM response proved to be highly specific for the infecting virus. In contrast, primary dengue virus infections induced substantial amounts of cross-reactive IgM antibodies, which is most likely explained by structural peculiarities of dengue virus particles. Despite the presence of cross-reactive IgM, the standardized nature and the quantitative read-out of the assay even allowed the serotype-specific diagnosis of recent dengue virus infections in most instances.

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