scholarly journals Robust Human and Murine Hepatocyte Culture Models of Hepatitis B Virus Infection and Replication

2018 ◽  
Vol 92 (23) ◽  
Author(s):  
Luhua Qiao ◽  
Jianhua Sui ◽  
Guangxiang Luo
Keyword(s):  
Cell Culture ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Cell Lines ◽  
Hbv Infection ◽  
Hepatitis B Virus Infection ◽  
B Virus ◽  
Culture Models ◽  
Cell Culture Models ◽  
Murine Hepatocyte

ABSTRACTHepatitis B virus (HBV) is a major cause of chronic liver diseases, including hepatitis, cirrhosis, and hepatocellular carcinoma. HBV research has been hampered by the lack of robust cell culture and small animal models of HBV infection. The discovery of sodium taurocholate cotransporting polypeptide (NTCP) as an HBV receptor has been a landmark advance in HBV research in recent years. Ectopic expression of NTCP in nonpermissive HepG2, Huh7, and AML12 cell lines confers HBV susceptibility. However, HBV replication in these human and murine hepatocyte cell lines appeared suboptimal. In the present study, we constructed stable NTCP-expressing HepG2 and AML12 cell lines and found that HBV permissiveness is correlated with NTCP expression. More significantly, we developed robust HBV cell culture models by treating the HBV-infected cells with dimethyl sulfoxide (DMSO) and hydrocortisone, which significantly promoted HBV replication and production. Mechanistic studies suggested that hydrocortisone significantly enhanced the transcription and expression of PGC1α and HNF4α, which are known to promote HBV transcription and replication. These new human and murine hepatocyte culture systems of HBV infection and replication will accelerate the determination of molecular aspects underlying HBV infection, replication, and morphogenesis in human and murine hepatocytes. We anticipate that our HBV cell culture models will also facilitate the discovery and development of antiviral drugs towards the ultimate eradication of chronic hepatitis B virus infection.IMPORTANCEHBV research has been greatly hampered by the lack of robust cell culture and small animal models of HBV infection and propagation. The discovery of NTCP as an HBV receptor has greatly impacted the field of HBV research. Although HBV infection of NTCP-expressing human and murine hepatocyte cell lines has been demonstrated, its replication in cell culture appeared inefficient. To further improve cell culture systems of HBV infection and replication, we constructed NTCP-expressing HepG2 and AML12 cell lines that are highly permissive to HBV infection. More significantly, we found that DMSO and hydrocortisone markedly enhanced HBV transcription and replication in human and murine hepatocytes when added to the cell culture medium. These new cell culture models of HBV infection and replication will facilitate HBV research and antiviral drug discovery towards the ultimate elimination of chronic hepatitis B virus infection.

scholarly journals Paternal hepatitis B virus infection with different clinical courses between siblings

2021 ◽  
Author(s):  
Yoshiaki Sasaki ◽  
Hiroki Kajino
Keyword(s):  
Hepatitis B Virus ◽  
Virus Infection ◽  
Hepatitis B ◽  
Family Members ◽  
Hbv Infection ◽  
Hepatitis B Virus Infection ◽  
Hbv Vaccination ◽  
B Virus

No countermeasures have been established against horizontal infection in non-vaccinated children. We reported about siblings with different clinical courses of HBV paternal infection. To eradicate HBV, we should encourage HBV vaccination of all children and HBV infection screening of fathers and other family members.

scholarly journals Hepatitis B Virus Replication Induces Methylation of both Host and Viral DNA

2010 ◽  
Vol 84 (9) ◽  
pp. 4321-4329 ◽  
Author(s):  
Perumal Vivekanandan ◽  
Hubert Darius-J Daniel ◽  
Rajesh Kannangai ◽  
Francisco Martinez-Murillo ◽  
Michael Torbenson
Keyword(s):  
Gene Expression ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Viral Replication ◽  
Cell Lines ◽  
Natural Infection ◽  
Cpg Islands ◽  
Hbv Infection ◽  
Viral Dna ◽  
B Virus

ABSTRACT Control of viral replication is a major therapeutic goal to reduce morbidity and mortality from chronic hepatitis B virus (HBV) infection. Recently, methylation has been identified as a novel host defense mechanism, and methylation of viral DNA leads to downregulation of HBV gene expression. To better understand the mechanisms of HBV methylation, cell lines were exposed to HBV using a model system that mimics natural infection and the expression of host DNA methyltransferase genes (DNMTs) was measured. DNMT1, DNMT2, and DNMT3 were all significantly upregulated in response to HBV. DNMT3 was further studied because of its known role in the de novo methylation of DNA. Cotransfection experiments with full-length HBV and DNMT3 led to the downregulation of viral protein and pregenomic RNA production. To investigate whether the upregulation of DNMTs could also have an effect on the methylation of host DNA, cell lines were exposed to HBV in two independent model systems, one that mimics natural infection and a second model with temporary transfection. Host DNA methylation was measured by DNA microarray analysis. Increased methylation of host CpG islands was detected in both experimental systems. Two CpG islands, corresponding to genes SUFU and TIRAP, were selected, and the downregulation of these genes in hepatocellular carcinomas was confirmed. In conclusion, hepatocytes respond to HBV infection by upregulating DNMTs. The DNMTs methylate viral DNA, leading to decreased viral gene expression and decreased viral replication. However, virus-induced overexpression of DNMTs also leads to methylation of host CpG islands.

Hepatitis B Virus Infection in Health Care Workers in Albania: a Country still Highly Endemic for HBV Infection

Infection ◽  
2007 ◽  
Vol 35 (2) ◽  
pp. 94-97 ◽  
Author(s):  
L. A. Kondili ◽  
D. Ulqinaku ◽  
M. Hajdini ◽  
M. Basho ◽  
P. Chionne ◽  
...  
Keyword(s):  
Health Care ◽  
Hepatitis B Virus ◽  
Virus Infection ◽  
Hepatitis B ◽  
Health Care Workers ◽  
Hbv Infection ◽  
Hepatitis B Virus Infection ◽  
Care Workers ◽  
B Virus

scholarly journals Prevalence and Knowledge of Hepatitis B Virus Infection among Pregnant Women in the Ningo-Prampram District, Ghana

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Precious Kwablah Kwadzokpui ◽  
Elliot Elikplim Akorsu ◽  
Albert Abaka-Yawson ◽  
Solomon Sosu Quarshie ◽  
Stephen Adomako Amankwah ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Virus Infection ◽  
Hepatitis B ◽  
Antenatal Care ◽  
Pregnant Women ◽  
Health Facility ◽  
Hbv Infection ◽  
Hepatitis B Virus Infection ◽  
B Virus ◽  
Study Participants

Background. Hepatitis B virus (HBV) infection has been suggested to play a role in various adverse birth outcomes. The study determined the prevalence as well as knowledge of hepatitis B virus infection among pregnant women in the Ningo-Prampram District of the Greater Accra Region of Ghana. Materials and Methods. A cross-sectional study using simple random sampling technique was used to recruit 213 pregnant women receiving antenatal care in three different health facilities (Prampram Polyclinic (PPC), Dangme Community Hospital (DCH), and Old Ningo Health Center (ONHC)) in the Ningo-Prampram District of Ghana from November 2018 to January 2019. A semi-structured questionnaire was used to collect data which included participants’ HBsAg test results, sociodemographic and gynaecological characteristics, and their level of knowledge on HBV infection. Knowledge of the participants on HBV infection was classified as either excellent, good, or poor based on their cumulative percentage scores from the questionnaire according to Al Rubaish system of classification. Results. Overall low-intermediate prevalence of HBV infection was 3.3%; however, PPC recorded the highest prevalence of 4.0% while DCH and ONHC recorded 2.82% and 2.50%, respectively. Statistically significant association was observed between HBV infection and the health facility. Majority (77.40%) of the study participants had poor knowledge on HBV infection while only 14 (6.57%) had excellent knowledge on HBV. Regarding excellent knowledge, 8 (11.0%) among the participants were demonstrated by the majority of those who received antenatal care from DCH. Generally, knowledge on HBV and the infection was poor among the study participants. Knowledge on HBV infection was found to be associated with residential status (p=0.006), educational level (p<0.001), occupation (p<0.001), and gestational period (p<0.001). Participant’s knowledge was also significantly associated with the health facility (p=0.027). Conclusion. HBV infection among pregnant women is prevalent in the Ningo-Prampram District even though the prevalence is not very high. The majority of pregnant women in the Ningo-Prampram District inadequate knowledge on HBV infection and it mode of transmission. Intensive public health education on the HBV infection is required in the district to help prevent and manage future transmissions as well as inform the population about the negative side effects of the virus and the need to prevent it by way of vaccination.

scholarly journals PP-116 Profile of occult hepatitis B virus infection in an area with intermediate prevalence of HBV infection

2009 ◽  
Vol 13 ◽  
pp. S80
Author(s):  
Shanmugam Saravanan ◽  
Vijayakumar Velu ◽  
Vidya Madhavan ◽  
Kailapuri G. Murugavel ◽  
Pachamuthu Balakrishnan ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Virus Infection ◽  
Hepatitis B ◽  
Hbv Infection ◽  
Hepatitis B Virus Infection ◽  
Occult Hepatitis ◽  
B Virus ◽  
Occult Hepatitis B
Sign in / Sign up

Export Citation Format

Share Document