Isolation and characterization of polyoma uncoating intermediates from the nuclei of infected mouse cells.

1980 ◽  
Vol 33 (3) ◽  
pp. 1173-1181 ◽  
Author(s):  
V D Winston ◽  
J B Bolen ◽  
R A Consigli
Keyword(s):  
Infected Mouse ◽  
Isolation And Characterization ◽  
Mouse Cells

scholarly journals The Isolation and Characterization of a Clonally Related Series of Murine Retrovirus-infected Mouse Cells

1980 ◽  
Vol 49 (1) ◽  
pp. 105-113 ◽  
Author(s):  
A. Morris ◽  
C. Clegg ◽  
J. Jones ◽  
B. Rodgers ◽  
R. J. Avery
Keyword(s):  
Infected Mouse ◽  
Isolation And Characterization ◽  
Mouse Cells ◽  
Related Series

scholarly journals ISOLATION AND CHARACTERIZATION OF C-TYPE VIRAL GENE PRODUCTS OF VIRUS-NEGATIVE MOUSE CELLS

1974 ◽  
Vol 139 (2) ◽  
pp. 427-438 ◽  
Author(s):  
John R. Stephenson ◽  
Steven R. Tronick ◽  
Roberta K. Reynolds ◽  
Stuart A. Aaronson
Keyword(s):  
Viral Gene ◽  
Gene Products ◽  
New Class ◽  
Viral Antigens ◽  
Isolation And Characterization ◽  
Cell Extracts ◽  
Mouse Cells ◽  
Specific Antigens ◽  
Low Levels

Antigens which immunologically cross-react with two mouse C-type viral polypeptides, p30 and p12, are present at very low levels in normal virus-negative mouse cells. These two antigens have been purified by 50–300-fold from cell extracts and shown to cochromatograph with the corresponding labeled viral polypeptides in several systems. Their type-specific antigenicities are shown to be distinct from those of previously tested MuLV isolates suggesting that they may be components of a new class of endogenous C-type virus. The methods utilized in the present studies for concentration of virus-specific antigens of normal mouse cells provide an approach for detection of C-type viral antigens in cells of other species.

scholarly journals Isolation and Characterization of BK Virus-Transformed Rat and Mouse Cells

1979 ◽  
Vol 42 (3) ◽  
pp. 567-578 ◽  
Author(s):  
J. Seehafer ◽  
D. N. Downer ◽  
A. Salmi ◽  
J. S. Colter
Keyword(s):  
Bk Virus ◽  
Isolation And Characterization ◽  
Mouse Cells

scholarly journals Isolation and characterization of sequences from mouse chromosomal DNA with ARS function in yeasts.

1983 ◽  
Vol 3 (11) ◽  
pp. 1898-1908 ◽  
Author(s):  
G E Roth ◽  
H M Blanton ◽  
L J Hager ◽  
V A Zakian
Keyword(s):  
Cell Lines ◽  
Mouse Tissue ◽  
Chromosomal Dna ◽  
Autonomously Replicating Sequence ◽  
Culture Cells ◽  
Isolation And Characterization ◽  
Multiple Copies ◽  
Mouse Cells ◽  
Simplex Virus

Fragments of chromosomal DNA from a variety of eucaryotes can act as ARSs (autonomously replicating sequence) in yeasts. ARSs enable plasmids to be maintained in extrachromosomal form, presumably because they function as initiation sites for DNA replication. We isolated eight different sequences from mouse chromosomal DNA which function as ARSs in Saccharomyces cerevisiae (bakers' yeast). Although the replication efficiency of the different mouse ARSs in yeasts appears to vary widely, about one-half of them functions as well as the yeast chromosomal sequence ARS1. Moreover, five of the ARSs also promote self replication of plasmids in Schizosaccharomyces pombe (fission yeast). Each of the ARSs was cloned into plasmids suitable for transformation of mouse tissue culture cells. Plasmids were introduced into thymidine kinase (TK)-deficient mouse L cells by the calcium phosphate precipitation technique in the absence of carrier DNA. In some experiments, the ARS plasmid contained the herpes simplex virus type 1 TK gene; in other experiments (cotransformations), the TK gene was carried on a separate plasmid used in the same transformation. In contrast to their behavior in yeasts, none of the ARS plasmids displayed a significant increase in transformation frequency in mouse cells compared with control plasmids. Moreover, only 1 of over 100 cell lines contained the original plasmid in extrachromosomal form. The majority of cell lines produced by transformation with an ARS TK plasmid contained multiple copies of plasmid integrated into chromosomal DNA. In most cases, results with plasmids used in cotransformations were similar to those for plasmids carrying TK. However, cell lines produced by cotransformations with plasmids containing any one of three of the ARSs (m24, m25, or m26) often contained extrachromosomal DNAs.

scholarly journals Cloning and Characterization of a Specific Receptor for Mouse Oncostatin M

1998 ◽  
Vol 18 (6) ◽  
pp. 3357-3367 ◽  
Author(s):  
Richard A. Lindberg ◽  
Todd S.-C. Juan ◽  
Andrew A. Welcher ◽  
Yu Sun ◽  
Rod Cupples ◽  
...  
Keyword(s):  
Protein Product ◽  
Oncostatin M ◽  
Ligand Specificity ◽  
Binding Studies ◽  
Isolation And Characterization ◽  
A Subunit ◽  
Mouse Cells ◽  
Species Specific ◽  
Nih 3T3

ABSTRACT Oncostatin M (OSM) is a member of a family of cytokines that includes ciliary neurotrophic factor, interleukin-6, interleukin-11, cardiotrophin-1, and leukemia inhibitory factor (LIF). The receptors for these cytokines consist of a common signaling subunit, gp130, to which other subunits are added to modify ligand specificity. We report here the isolation and characterization of a cDNA encoding a subunit of the mouse OSM receptor. In NIH 3T3 cells (which endogenously express gp130, LIF receptor β [LIFRβ], and the protein product, c12, of the cDNA described here), mouse LIF, human LIF, and human OSM signaled through receptors containing the LIFRβ and gp130 but not through the mouse OSM receptor. Mouse OSM, however, signaled only through a c12-gp130 complex; it did not use the LIF receptor. Binding studies demonstrated that mouse OSM associated directly with either the c12 protein or gp130. These data highlight the species-specific differences in receptor utilization and signal transduction between mouse and human OSM. In mouse cells, only mouse OSM is capable of activating the mouse OSM receptor; human OSM instead activates the LIF receptor. Therefore, these data suggest that all previous studies with human OSM in mouse systems did not elucidate the biology of OSM but, rather, reflected the biological actions of LIF.

Isolation and characterization of sequences from mouse chromosomal DNA with ARS function in yeasts

1983 ◽  
Vol 3 (11) ◽  
pp. 1898-1908
Author(s):  
G E Roth ◽  
H M Blanton ◽  
L J Hager ◽  
V A Zakian
Keyword(s):  
Cell Lines ◽  
Mouse Tissue ◽  
Chromosomal Dna ◽  
Autonomously Replicating Sequence ◽  
Culture Cells ◽  
Isolation And Characterization ◽  
Multiple Copies ◽  
Mouse Cells ◽  
Simplex Virus

Fragments of chromosomal DNA from a variety of eucaryotes can act as ARSs (autonomously replicating sequence) in yeasts. ARSs enable plasmids to be maintained in extrachromosomal form, presumably because they function as initiation sites for DNA replication. We isolated eight different sequences from mouse chromosomal DNA which function as ARSs in Saccharomyces cerevisiae (bakers' yeast). Although the replication efficiency of the different mouse ARSs in yeasts appears to vary widely, about one-half of them functions as well as the yeast chromosomal sequence ARS1. Moreover, five of the ARSs also promote self replication of plasmids in Schizosaccharomyces pombe (fission yeast). Each of the ARSs was cloned into plasmids suitable for transformation of mouse tissue culture cells. Plasmids were introduced into thymidine kinase (TK)-deficient mouse L cells by the calcium phosphate precipitation technique in the absence of carrier DNA. In some experiments, the ARS plasmid contained the herpes simplex virus type 1 TK gene; in other experiments (cotransformations), the TK gene was carried on a separate plasmid used in the same transformation. In contrast to their behavior in yeasts, none of the ARS plasmids displayed a significant increase in transformation frequency in mouse cells compared with control plasmids. Moreover, only 1 of over 100 cell lines contained the original plasmid in extrachromosomal form. The majority of cell lines produced by transformation with an ARS TK plasmid contained multiple copies of plasmid integrated into chromosomal DNA. In most cases, results with plasmids used in cotransformations were similar to those for plasmids carrying TK. However, cell lines produced by cotransformations with plasmids containing any one of three of the ARSs (m24, m25, or m26) often contained extrachromosomal DNAs.

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