scholarly journals Packaging Cell Lines for Simian Foamy Virus Type 1 Vectors

1999 ◽  
Vol 73 (5) ◽  
pp. 4498-4501 ◽  
Author(s):  
Min Wu ◽  
Ayalew Mergia
Keyword(s):  
Cell Line ◽  
Cell Lines ◽  
Virus Type ◽  
Foamy Virus ◽  
Vector System ◽  
Simian Foamy Virus ◽  
Packaging Cell Line ◽  
Packaging Cell ◽  
Foamy Viruses

ABSTRACT Foamy viruses are nonpathogenic retroviruses that offer several unique opportunities for gene transfer in various cell types from different species. We have previously demonstrated the utility of simian foamy virus type 1 (SFV-1) as a vector system by transient expression assay (M. Wu et al., J. Virol. 72:3451–3454, 1998). In this report, we describe the first stable packaging cell lines for foamy virus vectors based on SFV-1. We developed two packaging cell lines in which the helper DNA is placed under the control of either a constitutive cytomegalovirus (CMV) immediate-early gene or inducible tetracycline promoter for expression. Although the constitutive packaging expressing cell line had a higher copy number of packaging DNA, the inducible packaging cell line produced four times more vector particles. This result suggested that the structural gene products in the constitutively expressing packaging cell line were expressed at a level that is not toxic to the cells, and thus vector production was reduced. The SFV-1 vector in the presence of vesicular stomatitis virus envelope protein G (VSV-G) produced an insignificant level of transduction, indicating that foamy viruses could not be pseudotyped with VSV-G to generate high-titer vectors. The availability of stable packaging cell lines represents a step toward the use of an SFV-1 vector delivery system that will allow scaled-up production of vector stocks for gene therapy.

scholarly journals A Minimal Genome Simian Foamy Virus Type 1 Vector System with Efficient Gene Transfer

Virology ◽  
2002 ◽  
Vol 302 (2) ◽  
pp. 236-244 ◽  
Author(s):  
Jeonghae Park ◽  
Peter E. Nadeau ◽  
Ayalew Mergia
Keyword(s):  
Gene Transfer ◽  
Virus Type ◽  
Foamy Virus ◽  
Vector System ◽  
Simian Foamy Virus ◽  
Minimal Genome ◽  
Efficient Gene

scholarly journals Dual Simian Foamy Virus/Human Immunodeficiency Virus Type 1 Infections in Persons from Côte d’Ivoire

PLoS ONE ◽  
2016 ◽  
Vol 11 (6) ◽  
pp. e0157709 ◽  
Author(s):  
William M. Switzer ◽  
Shaohua Tang ◽  
HaoQiang Zheng ◽  
Anupama Shankar ◽  
Patrick S. Sprinkle ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Cote D'ivoire ◽  
Côte D’Ivoire ◽  
Foamy Virus ◽  
Simian Foamy Virus ◽  
Cote D’Ivoire ◽  
Immunodeficiency Virus

Sequence analysis of the simian foamy virus type 1 genome

Gene ◽  
1991 ◽  
Vol 101 (2) ◽  
pp. 185-194 ◽  
Author(s):  
Jean-Jacques Kupiec ◽  
Alan Kay ◽  
Maud Hayat ◽  
Rodica Ravier ◽  
Jorge Périés ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Virus Type ◽  
Foamy Virus ◽  
Simian Foamy Virus

scholarly journals Feasibility of Generating Adeno-Associated Virus Packaging Cell Lines Containing Inducible Adenovirus Helper Genes

2002 ◽  
Vol 76 (4) ◽  
pp. 1904-1913 ◽  
Author(s):  
Chunping Qiao ◽  
Juan Li ◽  
Anna Skold ◽  
Xudong Zhang ◽  
Xiao Xiao
Keyword(s):  
Cell Line ◽  
Cell Lines ◽  
Cost Effective ◽  
Helper Virus ◽  
Vector System ◽  
Wild Type ◽  
Aav Vector ◽  
Adeno Associated Virus ◽  
Packaging Cell ◽  
Producer Cell

ABSTRACT The adeno-associated virus (AAV) vector system is based on nonpathogenic and helper-virus-dependent parvoviruses. The vector system offers safe, efficient, and long-term in vivo gene transfer in numerous tissues. Clinical trials using AAV vectors have demonstrated vector safety as well as efficiency. The increasing interest in the use of AAV for clinical studies demands large quantities of vectors and hence a need for improvement in vector production. The commonly used transient-transfection method, although versatile and free of adenovirus (Ad), is not cost-effective for large-scale production. While the wild-type-Ad-dependent AAV producer cell lines seem to be cost-effective, this method faces the problem of wild-type Ad contamination. To overcome these shortcomings, we have explored the feasibility of creating inducible AAV packaging cell lines that require neither transfection nor helper virus infection. As a first step toward that goal, we have created a cell line containing highly inducible Ad E1A and E1B genes, which are essential for AAV production. Subsequently, the AAV Rep and Cap genes and an AAV vector containing a green fluorescent protein (GFP) reporter gene were stably introduced into the E1A-E1B cell line, generating inducible AAV-GFP packaging cell lines. Upon induction of E1A and E1B genes and infection with replication-defective Ad with E1A, E1B, and E3 deleted, the packaging cells yielded high-titer AAV-GFP vectors. Finally, the E2, E4, and VA genes of Ad, under the control of their endogenous promoters, were also introduced into these cells. A few producer cell lines were obtained, which could produce AAV-GFP vectors upon simple drug induction. Although future improvement is necessary to increase the stability and vector yield of the cells, our study has nonetheless demonstrated the feasibility of generating helper-virus-free inducible AAV producer cell lines.

scholarly journals cis-Acting Sequences Required for Simian Foamy Virus Type 1 Vectors

1998 ◽  
Vol 72 (4) ◽  
pp. 3451-3454 ◽  
Author(s):  
Min Wu ◽  
Soumya Chari ◽  
Tina Yanchis ◽  
Ayalew Mergia
Keyword(s):  
Gene Transfer ◽  
Virus Type ◽  
Foamy Virus ◽  
Gene Promoter ◽  
Early Gene ◽  
Leader Region ◽  
Simian Foamy Virus ◽  
Cis Acting ◽  
Vector Construction

ABSTRACT We have constructed a series of vectors based on simian foamy virus type 1 (SFV-1) to define the minimum cis-acting elements required for gene transfer. To characterize these vectors, we inserted the coding sequence of the bacterial lacZ gene linked to the cytomegalovirus immediate-early gene promoter. Introduction of a deletion mutation in the leader region between the 5′ long terminal repeat and the start of the gag gene at position 1659 to 1694 completely abrogated gene transfer by the SFV-1 vector. Deletion of 39 nucleotides from position 1692 to 1731 in the leader region resulted in a significant reduction in the transducing-particle titer. Furthermore, we have identified a second cis-acting element located at the 3′ end of the pol gene between position 6486 and 6975 to be critical for SFV-1 vector transduction. These results identify the two important cis-acting elements required for SFV-1 vector construction, and the finding of a cis-acting element in the pol gene is unique among retroviruses.

scholarly journals Inducible human immunodeficiency virus type 1 packaging cell lines.

1996 ◽  
Vol 70 (7) ◽  
pp. 4530-4537 ◽  
Author(s):  
H Yu ◽  
A B Rabson ◽  
M Kaul ◽  
Y Ron ◽  
J P Dougherty
Keyword(s):  
Human Immunodeficiency Virus ◽  
Cell Lines ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Packaging Cell ◽  
Immunodeficiency Virus

Characterization of RNase H activity associated with reverse transcriptase in simian foamy virus type 1.

1983 ◽  
Vol 47 (1) ◽  
pp. 249-252 ◽  
Author(s):  
A B Benzair ◽  
A Rhodes-Feuillette ◽  
R Emanoil-Ravicovitch ◽  
J Peries
Keyword(s):  
Reverse Transcriptase ◽  
Virus Type ◽  
Foamy Virus ◽  
Rnase H ◽  
Simian Foamy Virus

scholarly journals Purification and Characterization of the Major Envelope Glycoprotein of Simian Foamy Virus Type 1

1985 ◽  
Vol 66 (7) ◽  
pp. 1449-1455 ◽  
Author(s):  
A.-B. Benzair ◽  
A. Rhodes-Feuillette ◽  
J. Lasneret ◽  
R. Emanoil-Ravier ◽  
J. Peries
Keyword(s):  
Virus Type ◽  
Envelope Glycoprotein ◽  
Foamy Virus ◽  
Purification And Characterization ◽  
Simian Foamy Virus

scholarly journals An Immunodominant and Conserved B-Cell Epitope in the Envelope of Simian Foamy Virus Recognized by Humans Infected with Zoonotic Strains from Apes

2019 ◽  
Vol 93 (11) ◽  
Author(s):  
Caroline Lambert ◽  
Damien Batalie ◽  
Thomas Montange ◽  
Edouard Betsem ◽  
Augustin Mouinga-Ondeme ◽  
...  
Keyword(s):  
B Cell ◽  
Virus Type ◽  
Persistent Infection ◽  
Foamy Virus ◽  
Cell Epitope ◽  
Leader Peptide ◽  
Plasma Samples ◽  
B Cell Epitope ◽  
Foamy Viruses

ABSTRACTCross-species transmission of simian foamy viruses (SFVs) from nonhuman primates (NHPs) to humans is currently ongoing. These zoonotic retroviruses establish lifelong persistent infection in their human hosts. SFV are apparently nonpathogenicin vivo, with ubiquitousin vitrotropism. Here, we aimed to identify envelope B-cell epitopes that are recognized following a zoonotic SFV infection. We screened a library of 169 peptides covering the external portion of the envelope from the prototype foamy virus (SFVpsc_huHSRV.13) for recognition by samples from 52 Central African hunters (16 uninfected and 36 infected with chimpanzee, gorilla, orCercopithecusSFV). We demonstrate the specific recognition of peptide N96-V110located in the leader peptide, gp18LP. Forty-three variant peptides with truncations, alanine substitutions, or amino acid changes found in other SFV species were tested. We mapped the epitope between positions 98 and 108 and defined six amino acids essential for recognition. Most plasma samples from SFV-infected humans cross-reacted with sequences from apes and Old World monkey SFV species. The magnitude of binding to peptide N96-V110was significantly higher for samples of individuals infected with a chimpanzee or gorilla SFV than those infected with aCercopithecusSFV. In conclusion, we have been the first to define an immunodominant B-cell epitope recognized by humans following zoonotic SFV infection.IMPORTANCEFoamy viruses are the oldest known retroviruses and have been mostly described to be nonpathogenic in their natural animal hosts. SFVs can be transmitted to humans, in whom they establish persistent infection, like the simian lenti- and deltaviruses that led to the emergence of two major human pathogens, human immunodeficiency virus type 1 and human T-lymphotropic virus type 1. This is the first identification of an SFV-specific B-cell epitope recognized by human plasma samples. The immunodominant epitope lies in gp18LP, probably at the base of the envelope trimers. The NHP species the most genetically related to humans transmitted SFV strains that induced the strongest antibody responses. Importantly, this epitope is well conserved across SFV species that infect African and Asian NHPs.

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