A dominant negative mutation of the alpha retinoic acid receptor gene in a retinoic acid-nonresponsive embryonal carcinoma cell
1990 ◽
Vol 10
(12)
◽
pp. 6445-6453
Keyword(s):
Pluripotential embryonal carcinoma cells such as those of the P19 line differentiate when exposed to retinoic acid (RA). The RAC65 cell line is a mutant clone of P19 cells selected to be RA nonresponsive. RAC65 cells carry a rearrangement affecting one of the genes encoding a nuclear retinoic acid receptor (RAR alpha). The mutant gene encodes a protein, RAR alpha', that has lost its 70 C-terminal amino acids, thus truncating the RA-binding domain. The RAR alpha' was found to be a dominant repressor of transcription from an RA-responsive target gene; however, expression of RAR alpha' was insufficient to confer RA nonresponsiveness, suggesting that RAC65 cells carry an additional mutation(s) affecting RA-induced genes.
1990 ◽
Vol 10
(12)
◽
pp. 6445-6453
◽
1996 ◽
Vol 377
(11)
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pp. 703-710
1993 ◽
Vol 53
(2)
◽
pp. 105-113
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Keyword(s):
1997 ◽
Vol 56
(4)
◽
pp. 985-990
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1994 ◽
Vol 203
(5)
◽
pp. 254-265
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Keyword(s):
1993 ◽
Vol 157
(1)
◽
pp. 90-96
◽