scholarly journals Expression of p60v-src in Saccharomyces cerevisiae results in elevation of p34CDC28 kinase activity and release of the dependence of DNA replication on mitosis.

1993 ◽  
Vol 13 (8) ◽  
pp. 5112-5121 ◽  
Author(s):  
F Boschelli
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dna Replication ◽  
Dna Content ◽  
Protein Tyrosine Kinase ◽  
Flow Cytometric Analysis ◽  
Yeast Saccharomyces Cerevisiae ◽  
Genetic Studies ◽  
Flow Cytometric ◽  
Oncogenic Protein ◽  
Cdc28 Kinase

Expression of the oncogenic protein tyrosine kinase p60v-src in the yeast Saccharomyces cerevisiae has been shown to result in rapid cell death (J. S. Brugge, G. Jarosik, J. Andersen, A. Queral-Lustig, M. Fedor-Chaiken, and J. R. Broach, Mol. Cell. Biol. 7:2180-2187, 1987). Work described here demonstrates that v-Src expression results in accumulation of large-budded cells and a nuclear division block without blocking cytokinesis. Flow-cytometric analysis indicates that the DNA content of these cells is elevated beyond the G2 DNA content, and genetic studies indicate that v-Src expression causes aneuploidy. The activity of Cdc28 kinase, which controls the G1/S and G2/M transitions in S. cerevisiae, increases during galactose induction in a Src+ strain but not in an isogenic Src- strain. These observations indicate that v-Src expression disrupts p34CDC28 kinase regulation, allowing DNA replication to proceed in the absence of a prior mitotic event.

Expression of p60v-src in Saccharomyces cerevisiae results in elevation of p34CDC28 kinase activity and release of the dependence of DNA replication on mitosis

1993 ◽  
Vol 13 (8) ◽  
pp. 5112-5121
Author(s):  
F Boschelli
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dna Replication ◽  
Dna Content ◽  
Protein Tyrosine Kinase ◽  
Flow Cytometric Analysis ◽  
Yeast Saccharomyces Cerevisiae ◽  
Genetic Studies ◽  
Flow Cytometric ◽  
Oncogenic Protein ◽  
Cdc28 Kinase

Expression of the oncogenic protein tyrosine kinase p60v-src in the yeast Saccharomyces cerevisiae has been shown to result in rapid cell death (J. S. Brugge, G. Jarosik, J. Andersen, A. Queral-Lustig, M. Fedor-Chaiken, and J. R. Broach, Mol. Cell. Biol. 7:2180-2187, 1987). Work described here demonstrates that v-Src expression results in accumulation of large-budded cells and a nuclear division block without blocking cytokinesis. Flow-cytometric analysis indicates that the DNA content of these cells is elevated beyond the G2 DNA content, and genetic studies indicate that v-Src expression causes aneuploidy. The activity of Cdc28 kinase, which controls the G1/S and G2/M transitions in S. cerevisiae, increases during galactose induction in a Src+ strain but not in an isogenic Src- strain. These observations indicate that v-Src expression disrupts p34CDC28 kinase regulation, allowing DNA replication to proceed in the absence of a prior mitotic event.

scholarly journals Coupling DNA Replication and Spindle Function in Saccharomyces cerevisiae

Cells ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 3359
Author(s):  
Dimitris Liakopoulos
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dna Replication ◽  
Molecular Mechanisms ◽  
Genome Duplication ◽  
Yeast Cells ◽  
Yeast Saccharomyces Cerevisiae ◽  
Spindle Dynamics ◽  
Eukaryotic Organisms ◽  
Spindle Function

In the yeast Saccharomyces cerevisiae DNA replication and spindle assembly can overlap. Therefore, signaling mechanisms modulate spindle dynamics in order to ensure correct timing of chromosome segregation relative to genome duplication, especially when replication is incomplete or the DNA becomes damaged. This review focuses on the molecular mechanisms that coordinate DNA replication and spindle dynamics, as well as on the role of spindle-dependent forces in DNA repair. Understanding the coupling between genome duplication and spindle function in yeast cells can provide important insights into similar processes operating in other eukaryotic organisms, including humans.

scholarly journals Clinical Significance of Flow Cytometric Analysis of DNA Content in Colorectal Carcinoma.

1991 ◽  
Vol 24 (8) ◽  
pp. 2176-2182 ◽  
Author(s):  
Kazunori Tsujita ◽  
Kimihiko Funahashi ◽  
Masashi Watanabe ◽  
Hiroshi Nakamura ◽  
Kiyoshi Watanabe ◽  
...  
Keyword(s):  
Colorectal Carcinoma ◽  
Clinical Significance ◽  
Dna Content ◽  
Flow Cytometric Analysis ◽  
Flow Cytometric

scholarly journals Flow cytometric analysis DNA content in esophageal carcinoma. Correlation with histologic and clinical features

Cancer ◽  
1989 ◽  
Vol 64 (1) ◽  
pp. 80-82 ◽  
Author(s):  
Yu Jin-Ming ◽  
Yang Li-Hua ◽  
Guo-Qian ◽  
Chen Yan-Tiao ◽  
Li Xiao ◽  
...  
Keyword(s):  
Esophageal Carcinoma ◽  
Clinical Features ◽  
Dna Content ◽  
Flow Cytometric Analysis ◽  
Flow Cytometric

The lethality of p60v-src in Saccharomyces cerevisiae and the activation of p34CDC28 kinase are dependent on the integrity of the SH2 domain

1993 ◽  
Vol 105 (2) ◽  
pp. 519-528
Author(s):  
F. Boschelli ◽  
S.M. Uptain ◽  
J.J. Lightbody
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Cycle ◽  
Protein Tyrosine Kinase ◽  
Kinase Activity ◽  
Cell Cycle Control ◽  
Sh2 Domain ◽  
Wild Type ◽  
Type V ◽  
Cdc28 Kinase ◽  
In Cells

The lethal effects of the expression of the oncogenic protein tyrosine kinase p60v-src in Saccharomyces cerevisiae are associated with a loss of cell cycle control at the G1/S and G2/M checkpoints. Results described here indicate that the ability of v-Src to kill yeast is dependent on the integrity of the SH2 domain, a region of the Src protein involved in recognition of proteins phosphorylated on tyrosine. Catalytically active v-Src proteins with deletions in the SH2 domain have little effect on yeast growth, unlike wild-type v-Src protein, which causes accumulation of large-budded cells, perturbation of spindle microtubules and increased DNA content when expressed. The proteins phosphorylated on tyrosine in cells expressing v-Src differ from those in cells expressing a Src protein with a deletion in the SH2 domain. Also, unlike the wild-type v-Src protein, which drastically increases histone H1-associated Cdc28 kinase activity, c-Src and an altered v-Src protein have no effect on Cdc28 kinase activity. These results indicate that the SH2 domain is functionally important in the disruption of the yeast cell cycle by v-Src.

Three parameter flow cytometric analysis for simultaneous detection of cytokeratin, proliferation associated antigens and DNA content

Cytometry ◽  
1995 ◽  
Vol 21 (2) ◽  
pp. 177-186 ◽  
Author(s):  
Bert Schutte ◽  
Monique M. F. J. Tinnemans ◽  
Gio F. P. Pijpers ◽  
Marie-Hélène J. H. Lenders ◽  
Frans C. S. Ramaekers
Keyword(s):  
Dna Content ◽  
Flow Cytometric Analysis ◽  
Simultaneous Detection ◽  
Flow Cytometric

scholarly journals Flow cytometric analysis of the DNA content of gastric cancer

1987 ◽  
Vol 56 (1) ◽  
pp. 52-54 ◽  
Author(s):  
KC Ballantyne ◽  
PD James ◽  
RA Robins ◽  
RW Baldwin ◽  
JD Hardcastle
Keyword(s):  
Gastric Cancer ◽  
Dna Content ◽  
Flow Cytometric Analysis ◽  
Flow Cytometric

Flow cytometric analysis of nuclear DNA content in Musa

1999 ◽  
Vol 98 (8) ◽  
pp. 1344-1350 ◽  
Author(s):  
M. A. Lysák ◽  
M. Dolez˘elová ◽  
J. P. Horry ◽  
R. Swennen ◽  
J. Dolez˘el
Keyword(s):  
Dna Content ◽  
Nuclear Dna ◽  
Flow Cytometric Analysis ◽  
Nuclear Dna Content ◽  
Flow Cytometric

Flow cytometric analysis in colorectal carcinoma: prognostic significance of cellular DNA content

1990 ◽  
Vol 5 (4) ◽  
pp. 223-227 ◽  
Author(s):  
A. Schillaci ◽  
D. D. Tirindelli ◽  
M. Ferri ◽  
L. Teodori ◽  
F. Mauro ◽  
...  
Keyword(s):  
Colorectal Carcinoma ◽  
Dna Content ◽  
Prognostic Significance ◽  
Flow Cytometric Analysis ◽  
Flow Cytometric ◽  
Cellular Dna
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