scholarly journals Fatal Bilateral Chylothorax in Mice Lacking the Integrin α9β1

2000 ◽  
Vol 20 (14) ◽  
pp. 5208-5215 ◽  
Author(s):  
X. Z. Huang ◽  
J. F. Wu ◽  
R. Ferrando ◽  
J. H. Lee ◽  
Y. L. Wang ◽  
...  
Keyword(s):  
Respiratory Failure ◽  
Thoracic Duct ◽  
Lymphocytic Infiltration ◽  
Cell Matrix ◽  
Bilateral Chylothorax ◽  
Matrix Interactions ◽  
Congenital Chylothorax ◽  
Cell Matrix Interactions ◽  
Cell Adhesion Molecule 1

ABSTRACT Members of the integrin family of adhesion receptors mediate both cell-cell and cell-matrix interactions and have been shown to play vital roles in embryonic development, wound healing, metastasis, and other biological processes. The integrin α9β1 is a receptor for the extracellular matrix proteins osteopontin and tenacsin C and the cell surface immunoglobulin vascular cell adhesion molecule-1. This receptor is widely expressed in smooth muscle, hepatocytes, and some epithelia. To examine the in vivo function of α9β1, we have generated mice lacking expression of the α9 subunit. Mice homozygous for a null mutation in the α9 subunit gene appear normal at birth but develop respiratory failure and die between 6 and 12 days of age. The respiratory failure is caused by an accumulation of large volumes of pleural fluid which is rich in triglyceride, cholesterol, and lymphocytes. α9 −/− mice also develop edema and lymphocytic infiltration in the chest wall that appears to originate around lymphatics. α9 protein is transiently expressed in the developing thoracic duct at embryonic day 14, but expression is rapidly lost during later stages of development. Our results suggest that the α9 integrin is required for the normal development of the lymphatic system, including the thoracic duct, and that α9 deficiency could be one cause of congenital chylothorax.

Different mechanisms in the attachment of cells to native and denatured collagen

1979 ◽  
Vol 38 (1) ◽  
pp. 267-281
Author(s):  
S.L. Schor ◽  
J. Court
Keyword(s):  
Cell Attachment ◽  
Experimental Conditions ◽  
Cell Matrix ◽  
Matrix Interactions ◽  
Independent Mechanism ◽  
Cell Matrix Interactions ◽  
Serum Dependent ◽  
Kinetics Of ◽  
Dependent Mechanism

The attachment of cells to collagen has been reported previously to require the presence of serum and the particular serum protein involved in this process, variously known as CIG, CAP or fibronectin, has been isolated. This conclusion that cell attachment to collagen requires serum (or more precisely, fibronectin) is based on experiments measuring the kinetics of cell attachment to films of collagen. We have measured the kinetics of attachment of HeLa and attachment to films of collagen-containing substrata under a variety of experimental conditions and present evidence that the serum-dependent mechanism of cell attachment described by others is actually only the case for films of denatured collagen, while cell attachment to native collagen fibres occurs by a different, serum-independent, mechanism. The possible relevance of these findings to cell-matrix interactions in vivo is discussed.

scholarly journals Cell-matrix interactions during development and apoptosis of the mouse mammary gland in vivo

2002 ◽  
Vol 223 (4) ◽  
pp. 497-516 ◽  
Author(s):  
Janine M. Prince ◽  
Teresa C.M. Klinowska ◽  
Emma Marshman ◽  
Emma T. Lowe ◽  
Ulrike Mayer ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Mouse Mammary Gland ◽  
Cell Matrix ◽  
Matrix Interactions ◽  
Cell Matrix Interactions

scholarly journals Guiding oligodendrocyte precursor cell maturation with urokinase plasminogen activator-degradable elastin-like protein hydrogels

2020 ◽  
Author(s):  
Edi Meco ◽  
W. Sharon Zheng ◽  
Anahita H. Sharma ◽  
Kyle J. Lampe
Keyword(s):  
Plasminogen Activator ◽  
Urokinase Plasminogen Activator ◽  
Cell Matrix ◽  
Matrix Interactions ◽  
The Central Nervous System ◽  
Oligodendrocyte Precursor ◽  
Cell Matrix Interactions ◽  
Developing Rat

AbstractDemyelinating injuries and diseases, like multiple sclerosis, affect millions of people worldwide. Oligodendrocyte precursor cells (OPCs) have the potential to repair demyelinated tissue because they can both self-renew and differentiate into oligodendrocytes (OLs), the myelin producing cells of the central nervous system (CNS). Cell-matrix interactions impact OPC differentiation into OLs, but the process is not fully understood. Biomaterial hydrogel systems help to elucidate cell-matrix interactions because they can mimic specific properties of native CNS tissue in an in vitro setting. We investigated whether OPC maturation into OLs is influenced by interacting with a urokinase plasminogen activator (uPA) degradable extracellular matrix (ECM). uPA is a proteolytic enzyme that is transiently upregulated in the developing rat brain, with peak uPA expression correlating with an increase in myelin production in vivo. OPC-like cells isolated through the Mosaic Analysis with Double Marker technique (MADM OPCs) produced low molecular weight uPA in culture. MADM OPCs were encapsulated into two otherwise similar elastin-like protein (ELP) hydrogel systems: one that was uPA degradable and one that was non-degradable. Encapsulated MADM OPCs had similar viability, proliferation, and metabolic activity in uPA degradable and non-degradable ELP hydrogels. Expression of OPC maturation-associated genes, however, indicated that uPA degradable ELP hydrogels promoted MADM OPC maturation although not sufficiently for these cells to differentiate into OLs.Graphical Abstract – For table of contents only

scholarly journals Beta-catenin expression in Dupuytren's disease: potential role for cell–matrix interactions in modulating beta-catenin levels in vivo and in vitro

Oncogene ◽  
2003 ◽  
Vol 22 (24) ◽  
pp. 3680-3684 ◽  
Author(s):  
Vincenzo M Varallo ◽  
Bing Siang Gan ◽  
Shannon Seney ◽  
Douglas C Ross ◽  
James H Roth ◽  
...  
Keyword(s):  
Potential Role ◽  
Dupuytren’S Disease ◽  
Beta Catenin ◽  
Dupuytren's Disease ◽  
Cell Matrix ◽  
Matrix Interactions ◽  
Cell Matrix Interactions

scholarly journals Nanocellulose-Based Inks for 3D Bioprinting: Key Aspects in Research Development and Challenging Perspectives in Applications—A Mini Review

2020 ◽  
Vol 7 (2) ◽  
pp. 40 ◽  
Author(s):  
Xiaoju Wang ◽  
Qingbo Wang ◽  
Chunlin Xu
Keyword(s):  
Cellulose Nanofibers ◽  
Structural Similarity ◽  
3D Bioprinting ◽  
Mechanical Stiffness ◽  
In Vivo Models ◽  
Cell Matrix ◽  
Matrix Interactions ◽  
Cell Matrix Interactions ◽  
The Impact

Nanocelluloses have emerged as a catalogue of renewable nanomaterials for bioink formulation in service of 3D bioprinting, thanks to their structural similarity to extracellular matrices and excellent biocompatibility of supporting crucial cellular activities. From a material scientist’s viewpoint, this mini-review presents the key research aspects of the development of the nanocellulose-based bioinks in 3D (bio)printing. The nanomaterial properties of various types of nanocelluloses, including bacterial nanocellulose, cellulose nanofibers, and cellulose nanocrystals, are reviewed with respect to their origins and preparation methods. Different cross-linking strategies to integrate into multicomponent nanocellulose-based bioinks are discussed in terms of regulating ink fidelity in direct ink writing as well as tuning the mechanical stiffness as a bioactive cue in the printed hydrogel construct. Furthermore, the impact of surface charge and functional groups on nanocellulose surface on the crucial cellular activities (e.g., cell survival, attachment, and proliferation) is discussed with the cell–matrix interactions in focus. Aiming at a sustainable and cost-effective alternative for end-users in biomedical and pharmaceutical fields, challenging aspects such as biodegradability and potential nanotoxicity of nanocelluloses call for more fundamental comprehension of the cell–matrix interactions and further validation in in vivo models.

scholarly journals A 3-D in vitro co-culture model of mammary gland involution

2014 ◽  
Vol 6 (6) ◽  
pp. 618-626 ◽  
Author(s):  
Jonathan J. Campbell ◽  
Laur-Alexandru Botos ◽  
Timothy J. Sargeant ◽  
Natalia Davidenko ◽  
Ruth E. Cameron ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Hormonal Control ◽  
Tissue Formation ◽  
Cell Matrix ◽  
Mammary Gland Involution ◽  
Culture Model ◽  
Matrix Interactions ◽  
Cell Matrix Interactions

An in vitro model of mammary gland supporting 3D cell–cell and cell–matrix interactions demonstrates complete in vivo-like neo-tissue formation and remodelling processes (involution) under hormonal control.

scholarly journals Advances in multicellular spheroids formation

2017 ◽  
Vol 14 (127) ◽  
pp. 20160877 ◽  
Author(s):  
X. Cui ◽  
Y. Hartanto ◽  
H. Zhang
Keyword(s):  
Three Dimensional ◽  
Confined Space ◽  
Multicellular Spheroids ◽  
Fundamental Building Block ◽  
Cell Matrix ◽  
Matrix Interactions ◽  
Cell Matrix Interactions ◽  
Cell Cell

Three-dimensional multicellular spheroids (MCSs) have a complex architectural structure, dynamic cell–cell/cell–matrix interactions and bio-mimicking in vivo microenvironment. As a fundamental building block for tissue reconstruction, MCSs have emerged as a powerful tool to narrow down the gap between the in vitro and in vivo model. In this review paper, we discussed the structure and biology of MCSs and detailed fabricating methods. Among these methods, the approach in microfluidics with hydrogel support for MCS formation is promising because it allows essential cell–cell/cell–matrix interactions in a confined space.

Attachment of sponge cells to collagen substrata: effect of a collagen assembly factor

1985 ◽  
Vol 79 (1) ◽  
pp. 271-285
Author(s):  
B. Diehl-Seifert ◽  
B. Kurelec ◽  
R.K. Zahn ◽  
A. Dorn ◽  
B. Jericevic ◽  
...  
Keyword(s):  
Cell Attachment ◽  
Cell Matrix ◽  
Sequence Of Events ◽  
Matrix Interactions ◽  
Geodia Cydonium ◽  
Aggregation Factor ◽  
Assembly Factor ◽  
Electron Microscopical ◽  
Cell Matrix Interactions

Collagen, isolated from the sponge Geodia cydonium in the absence of denaturing agents, had the typical amino acid composition and was associated with the carbohydrates galactose and glucose. The resulting individual fibrils with a diameter of 23 nm, displayed a 19.5 nm periodicity with one intraperiod band. A collagen assembly factor (CAF) was identified in and partially purified from the extracellular space. The CAF reacted with antibodies against intact Geodia cells but not with antibodies against Geodia lectin and Geodia aggregation factor. In the presence of the CAF, the collagen fibrils reconstituted collagen bundles in an ordered sequence of events, which were followed by electron-microscopical and biochemical methods. Bundle formation was not dependent on the presence of the homologous lectin, glycoconjugates or aggregation factor. Homologous cells (Geodia archaeocytes) were determined to attach only to those Geodia collagen substrates that contained CAF. The attachment of these cells did not require fibronectin or Geodia lectin. Homologous glycoconjugates or NaOH-treated collagen inhibited cell attachment. Collagen from the sponge Chondrosia reniformis, even in the presence of Geodia CAF, was no appropriate substrate for Geodia cell attachment. Whether collagen is a component of cell-matrix interactions in sponge systems also in vivo is discussed.

scholarly journals What Molecular Recognition Systems Do Mesenchymal Stem Cells/Medicinal Signaling Cells (MSC) Use to Facilitate Cell-Cell and Cell Matrix Interactions? A Review of Evidence and Options

2021 ◽  
Vol 22 (16) ◽  
pp. 8637
Author(s):  
David A. Hart
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Molecular Recognition ◽  
Tissue Repair ◽  
Bone Cells ◽  
Cell Matrix ◽  
Matrix Interactions ◽  
Recognition Systems ◽  
Cell Matrix Interactions

Mesenchymal stem cells, also called medicinal signaling cells (MSC), have been studied regarding their potential to facilitate tissue repair for >30 years. Such cells, derived from multiple tissues and species, are capable of differentiation to a number of lineages (chondrocytes, adipocytes, bone cells). However, MSC are believed to be quite heterogeneous with regard to several characteristics, and the large number of studies performed thus far have met with limited or restricted success. Thus, there is more to understand about these cells, including the molecular recognition systems that are used by these cells to perform their functions, to enhance the realization of their potential to effect tissue repair. This perspective article reviews what is known regarding the recognition systems available to MSC, the possible systems that could be looked for, and alternatives to enhance their localization to specific injury sites and increase their subsequent facilitation of tissue repair. MSC are reported to express recognition molecules of the integrin family. However, there are a number of other recognition molecules that also could be involved such as lectins, inducible lectins, or even a MSC-specific family of molecules unique to these cells. Finally, it may be possible to engineer expression of recognition molecules on the surface of MSC to enhance their function in vivo artificially. Thus, improved understanding of recognition molecules on MSC could further their success in fostering tissue repair.

Sign in / Sign up

Export Citation Format

Share Document