Error-prone mutagenesis detected in mammalian cells by a shuttle vector containing the supF gene of Escherichia coli.
1984 ◽
Vol 4
(10)
◽
pp. 2227-2230
◽
Keyword(s):
When a shuttle vector containing a tyrosine suppressor tRNA (supF) gene as a target for mutagenesis replicated in a monkey kidney cell line, the frequency of SupF+ mutations was 2.3 +/- 0.5 x 10(-3). When the host cells were treated with ethyl methanesulfonate 40 h before transfection, a 10-fold increase in SupF+ mutation frequency was observed. These results supported the hypothesis that a damage-inducible mutagenic pathway exists in mammalian cells and also demonstrated the utility of this shuttle vector for the study of mutagenesis in mammalian cells.
1984 ◽
Vol 4
(10)
◽
pp. 2227-2230
Keyword(s):
1994 ◽
Vol 323
(3)
◽
pp. 105-112
◽
Keyword(s):
Keyword(s):
1991 ◽
Vol 5
(5-6)
◽
pp. 435-438
◽
Keyword(s):
1980 ◽
Vol 168
(4)
◽
pp. 239-248
◽
Keyword(s):
1985 ◽
Vol 846
(1)
◽
pp. 120-126
◽
Keyword(s):
Keyword(s):
1982 ◽
Vol 711
(3)
◽
pp. 466-477
◽
Keyword(s):
1988 ◽
Vol 8
(12)
◽
pp. 5425-5431
Keyword(s):