scholarly journals Tissue-specific and hormonal regulation of the gene for rat prostatic steroid-binding protein in transgenic mice.

1989 ◽  
Vol 9 (5) ◽  
pp. 2254-2257 ◽  
Author(s):  
J Allison ◽  
Y L Zhang ◽  
M G Parker
Keyword(s):  
Transgenic Mice ◽  
Hormonal Regulation ◽  
Binding Protein ◽  
Ventral Prostate ◽  
Tissue Specific ◽  
Regulated Expression ◽  
Selective Expression ◽  
Flanking Sequences ◽  
Steroid Binding ◽  
Steroid Binding Protein

We investigated the tissue-specific and hormonal regulation of the gene for rat prostatic steroid-binding protein by introducing the C3(1) gene with 4-kilobase (kb) upstream and 2-kb downstream flanking sequences into transgenic mice. There was selective expression in the ventral prostate that was stimulated by testosterone, which indicated that the gene together with 6-kb flanking DNA contains the information required for prostate-specific and testosterone-regulated expression.

Tissue-specific and hormonal regulation of the gene for rat prostatic steroid-binding protein in transgenic mice

1989 ◽  
Vol 9 (5) ◽  
pp. 2254-2257
Author(s):  
J Allison ◽  
Y L Zhang ◽  
M G Parker
Keyword(s):  
Transgenic Mice ◽  
Hormonal Regulation ◽  
Binding Protein ◽  
Ventral Prostate ◽  
Tissue Specific ◽  
Regulated Expression ◽  
Selective Expression ◽  
Flanking Sequences ◽  
Steroid Binding ◽  
Steroid Binding Protein

We investigated the tissue-specific and hormonal regulation of the gene for rat prostatic steroid-binding protein by introducing the C3(1) gene with 4-kilobase (kb) upstream and 2-kb downstream flanking sequences into transgenic mice. There was selective expression in the ventral prostate that was stimulated by testosterone, which indicated that the gene together with 6-kb flanking DNA contains the information required for prostate-specific and testosterone-regulated expression.

Expression of androgen receptors and prostatic steroid-binding protein during development of the rat ventral prostate

1988 ◽  
Vol 117 (3) ◽  
pp. 361-NP ◽  
Author(s):  
Y. L. Zhang ◽  
Z. X. Zhou ◽  
Y. D. Zhang ◽  
M. G. Parker
Keyword(s):  
Gene Expression ◽  
Androgen Receptors ◽  
Binding Protein ◽  
Transient Increase ◽  
Neonatal Rat ◽  
Ventral Prostate ◽  
Rat Ventral Prostate ◽  
Old Rats ◽  
Steroid Binding ◽  
Steroid Binding Protein

ABSTRACT Prostatic steroid-binding protein (PSBP) mRNAs transcribed from the three genes C1, C2 and C3 were quantitated in neonatal rat ventral prostate by Northern blot analysis. Transcription was initiated at day 14 for C1 and C2 and day 10 for C3, and reached mature levels by day 21 for C1 and C2 and day 28 for C3. The changes of both cytoplasmic and nuclear prostatic androgen receptors in 10- to 150-day-old rats were investigated by radioligand assay and showed a fivefold transient increase between days 10 and 28. Thus there was a good correlation between the onset of PSBP gene expression and the transient increase in androgen receptors; increases in receptor concentration may be a prerequisite for changes in gene expression. J. Endocr. (1988) 117, 361–366

Transcription of prostatic steroid binding protein (PSBP) gene is induced by epithelial-mesenchymal interaction

Development ◽  
1990 ◽  
Vol 110 (1) ◽  
pp. 273-281 ◽  
Author(s):  
H. Takeda ◽  
N. Suematsu ◽  
T. Mizuno
Keyword(s):  
In Situ Hybridization ◽  
Urinary Bladder ◽  
Binding Protein ◽  
Ventral Prostate ◽  
Northern Analysis ◽  
Specific Differentiation ◽  
Steroid Binding ◽  
Recombination Experiments ◽  
Steroid Binding Protein

The prostate gland develops from the fetal urogenital sinus at the base of the urinary bladder. It finally differentiates into three lobes; ventral, lateral and dorsal lobes of the prostate. In spite of their common developmental origin and similar glandular structure, these lobes show the different biochemical characteristics, for example, in the proteins they secrete. In the present study, we investigate the involvement of the epithelial-mesenchymal interaction in the lobe-specific differentiation of the prostatic epithelium by means of epithelial-mesenchymal recombination experiments. We have used a prostatic steroid-binding protein (PSBP) as a specific differentiation marker for the ventral prostate. PSBP is a tetramer which consists of 2 sub-units, one containing the polypeptides C1 and C3 and the other containing the polypeptides C2 and C3. Northern analysis with a complementary DNA probe encoding C1 peptide (PSBP-C1) revealed that the mRNAs were detected exclusively in the ventral prostate but not in the dorsal prostate or in other organs such as urinary bladder and kidney. In situ hybridization with a complementary (anti-sense) RNA probe demonstrated that the transcripts were found only in the epithelium, not in the mesenchyme of the ventral prostate. In situ hybridization also showed that, in normal development, the mRNAs for PSBP-C1 in the ventral epithelium were first detectable at day 14 after birth, coinciding with the onset of its cytodifferentiation, and that they reached mature levels by day 21. We then carried out tissue-recombination experiments to examine whether the transcription of the PSBP-C1 gene in the epithelium is affected by the surrounding mesenchyme. Fetal urogenital sinuses were subdivided into ventral and dorsal halves. Following collagenase treatment, both halves were separated into their epithelial and mesenchymal compartments. Homotypic (ventral epithelium plus ventral mesenchyme [Ev/Mv] and dorsal epithelium plus dorsal mesenchyme [Ed/Md]) and heterotypic (ventral epithelium plus dorsal mesenchyme [Ev/Md] and dorsal epithelium plus ventral mesenchyme [Ed/Mv]) recombinations were carried out. After 4–5 weeks of growth in male host, the glandular structures characteristic for prostate glands were formed in all explants. However, in situ hybridization revealed the transcripts of the PSBP-C1 gene only in the epithelium associated with the ventral mesenchyme (Ev/Mv and Ed/Mv).(ABSTRACT TRUNCATED AT 400 WORDS)

Foetal steroid binding protein in British and Japanese women

1987 ◽  
Vol 114 (4) ◽  
pp. 584-588 ◽  
Author(s):  
M. Jawed Iqbal ◽  
Alastair Forbes ◽  
Mark L. Wilkinson ◽  
John W. Moore ◽  
Roger Williams ◽  
...  
Keyword(s):  
Ovarian Function ◽  
Binding Protein ◽  
Premenopausal Women ◽  
Japanese Women ◽  
Sex Hormone Binding Globulin ◽  
Partial Purification ◽  
Enzyme Linked Immunosorbent Assay ◽  
Binding Characteristics ◽  
Steroid Binding ◽  
Steroid Binding Protein

Abstract. In order to examine the newly-discovered sex-steroid binding protein, foetal steroid binding protein (FSBP) in different populations, its binding characteristics and its level were studied by two-tier column ligand binding assay and enzyme-linked immunosorbent assay (ELISA) respectively. In 10 Japanese premenopausal women, analysis of 5α-dihydrotestosterone (DHT) binding in the Cibacron Blue 3GA-Sepharose 6B portion of the column showed a rising plateau pattern with a mean maximum binding of 31.1 ± 7.41%, whereas of 9 similar British women, 8 displayed unsaturable, non-cooperative binding of 11.6 ± 8.22% (P < 0.01). After partial purification of FSBP in these samples, the protein exhibited saturable binding kinetics, median binding 25 (interquartiles 23–34) and 19 (13–25) nmol DHT/l in Japanese and British women, respectively (P < 0.05). By analyzing FSBP by ELISA in 56 Japanese (45 premenopausal) and 59 British (25 premenopausal) women, higher levels were obtained in the whole Japanese group (P = 0.0016) and in the premenopausal Japanese women (P = 0.018) than in their British counterparts. In both nationalities, FSBP levels were higher in premenopausal women, and there was a significant negative correlation of FSBP with age in both populations, particularly in postmenopausal women. FSBP levels did not correlate with weight, parity, sex hormone binding globulin or albumin levels. The influence of FSBP on free steroid levels remains unclear, but some relationship with ovarian function seems a possibility.

scholarly journals The putative molybdate-stabilized progesterone receptor subunit is not a steroid-binding protein.

1984 ◽  
Vol 259 (2) ◽  
pp. 1091-1098
Author(s):  
M Birnbaumer ◽  
R C Bell ◽  
W T Schrader ◽  
B W O'Malley
Keyword(s):  
Progesterone Receptor ◽  
Binding Protein ◽  
Receptor Subunit ◽  
Steroid Binding ◽  
Steroid Binding Protein

Effects of sex steroids on regulation of the levels of Cl peptide of rat prostatic steroid-binding protein mRNA evaluated by in-situ hybridization

1988 ◽  
Vol 1 (3) ◽  
pp. 213-223 ◽  
Author(s):  
G. Pelletier ◽  
C. Labrie ◽  
J. Simard ◽  
M. Duval ◽  
M. G. Martinoli ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Sex Steroids ◽  
Binding Protein ◽  
Steroid Binding ◽  
Steroid Binding Protein

Amino acid sequence of the sex steroid binding protein of human blood plasma

Biochemistry ◽  
1986 ◽  
Vol 25 (23) ◽  
pp. 7584-7590 ◽  
Author(s):  
Kenneth A. Walsh ◽  
Koiti Titani ◽  
Koji Takio ◽  
Santosh Kumar ◽  
Rutherford Hayes ◽  
...  
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Blood Plasma ◽  
Human Blood ◽  
Binding Protein ◽  
Sex Steroid ◽  
Human Blood Plasma ◽  
Steroid Binding ◽  
Steroid Binding Protein
Sign in / Sign up

Export Citation Format

Share Document