Effects of sex steroids on regulation of the levels of Cl peptide of rat prostatic steroid-binding protein mRNA evaluated by in-situ hybridization

1988 ◽  
Vol 1 (3) ◽  
pp. 213-223 ◽  
Author(s):  
G. Pelletier ◽  
C. Labrie ◽  
J. Simard ◽  
M. Duval ◽  
M. G. Martinoli ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Sex Steroids ◽  
Binding Protein ◽  
Steroid Binding ◽  
Steroid Binding Protein

Transcription of prostatic steroid binding protein (PSBP) gene is induced by epithelial-mesenchymal interaction

Development ◽  
1990 ◽  
Vol 110 (1) ◽  
pp. 273-281 ◽  
Author(s):  
H. Takeda ◽  
N. Suematsu ◽  
T. Mizuno
Keyword(s):  
In Situ Hybridization ◽  
Urinary Bladder ◽  
Binding Protein ◽  
Ventral Prostate ◽  
Northern Analysis ◽  
Specific Differentiation ◽  
Steroid Binding ◽  
Recombination Experiments ◽  
Steroid Binding Protein

The prostate gland develops from the fetal urogenital sinus at the base of the urinary bladder. It finally differentiates into three lobes; ventral, lateral and dorsal lobes of the prostate. In spite of their common developmental origin and similar glandular structure, these lobes show the different biochemical characteristics, for example, in the proteins they secrete. In the present study, we investigate the involvement of the epithelial-mesenchymal interaction in the lobe-specific differentiation of the prostatic epithelium by means of epithelial-mesenchymal recombination experiments. We have used a prostatic steroid-binding protein (PSBP) as a specific differentiation marker for the ventral prostate. PSBP is a tetramer which consists of 2 sub-units, one containing the polypeptides C1 and C3 and the other containing the polypeptides C2 and C3. Northern analysis with a complementary DNA probe encoding C1 peptide (PSBP-C1) revealed that the mRNAs were detected exclusively in the ventral prostate but not in the dorsal prostate or in other organs such as urinary bladder and kidney. In situ hybridization with a complementary (anti-sense) RNA probe demonstrated that the transcripts were found only in the epithelium, not in the mesenchyme of the ventral prostate. In situ hybridization also showed that, in normal development, the mRNAs for PSBP-C1 in the ventral epithelium were first detectable at day 14 after birth, coinciding with the onset of its cytodifferentiation, and that they reached mature levels by day 21. We then carried out tissue-recombination experiments to examine whether the transcription of the PSBP-C1 gene in the epithelium is affected by the surrounding mesenchyme. Fetal urogenital sinuses were subdivided into ventral and dorsal halves. Following collagenase treatment, both halves were separated into their epithelial and mesenchymal compartments. Homotypic (ventral epithelium plus ventral mesenchyme [Ev/Mv] and dorsal epithelium plus dorsal mesenchyme [Ed/Md]) and heterotypic (ventral epithelium plus dorsal mesenchyme [Ev/Md] and dorsal epithelium plus ventral mesenchyme [Ed/Mv]) recombinations were carried out. After 4–5 weeks of growth in male host, the glandular structures characteristic for prostate glands were formed in all explants. However, in situ hybridization revealed the transcripts of the PSBP-C1 gene only in the epithelium associated with the ventral mesenchyme (Ev/Mv and Ed/Mv).(ABSTRACT TRUNCATED AT 400 WORDS)

Foetal steroid binding protein in British and Japanese women

1987 ◽  
Vol 114 (4) ◽  
pp. 584-588 ◽  
Author(s):  
M. Jawed Iqbal ◽  
Alastair Forbes ◽  
Mark L. Wilkinson ◽  
John W. Moore ◽  
Roger Williams ◽  
...  
Keyword(s):  
Ovarian Function ◽  
Binding Protein ◽  
Premenopausal Women ◽  
Japanese Women ◽  
Sex Hormone Binding Globulin ◽  
Partial Purification ◽  
Enzyme Linked Immunosorbent Assay ◽  
Binding Characteristics ◽  
Steroid Binding ◽  
Steroid Binding Protein

Abstract. In order to examine the newly-discovered sex-steroid binding protein, foetal steroid binding protein (FSBP) in different populations, its binding characteristics and its level were studied by two-tier column ligand binding assay and enzyme-linked immunosorbent assay (ELISA) respectively. In 10 Japanese premenopausal women, analysis of 5α-dihydrotestosterone (DHT) binding in the Cibacron Blue 3GA-Sepharose 6B portion of the column showed a rising plateau pattern with a mean maximum binding of 31.1 ± 7.41%, whereas of 9 similar British women, 8 displayed unsaturable, non-cooperative binding of 11.6 ± 8.22% (P < 0.01). After partial purification of FSBP in these samples, the protein exhibited saturable binding kinetics, median binding 25 (interquartiles 23–34) and 19 (13–25) nmol DHT/l in Japanese and British women, respectively (P < 0.05). By analyzing FSBP by ELISA in 56 Japanese (45 premenopausal) and 59 British (25 premenopausal) women, higher levels were obtained in the whole Japanese group (P = 0.0016) and in the premenopausal Japanese women (P = 0.018) than in their British counterparts. In both nationalities, FSBP levels were higher in premenopausal women, and there was a significant negative correlation of FSBP with age in both populations, particularly in postmenopausal women. FSBP levels did not correlate with weight, parity, sex hormone binding globulin or albumin levels. The influence of FSBP on free steroid levels remains unclear, but some relationship with ovarian function seems a possibility.

scholarly journals In situ detection of vitamin D-induced calcium-binding protein (9-kDa CaBP) messenger RNA in rat duodenum.

1986 ◽  
Vol 34 (2) ◽  
pp. 277-280 ◽  
Author(s):  
M Warembourg ◽  
O Tranchant ◽  
C Perret ◽  
C Desplan ◽  
M Thomasset
Keyword(s):  
Vitamin D ◽  
In Situ Hybridization ◽  
Calcium Binding ◽  
Binding Protein ◽  
Cdna Probe ◽  
Calcium Binding Protein ◽  
Pbr322 Dna ◽  
Rat Duodenum ◽  
Columnar Cells

We have previously described the molecular cloning of a cDNA fragment synthesized from rat duodenal mRNA coding for a 9000-dalton vitamin D-induced calcium-binding protein (9-kDa CaBP) (3). We now report the use of this cloned cDNA to study the cytological distribution of 9-kDa CaBP mRNA in rat duodenum by in situ hybridization. Tissue sections, fixed in ethanol:acetic acid, were hybridized to the 3H-cDNA probe and processed for autoradiography. The specificity of the CaBP mRNA-DNA hybrid formation was checked using 3H-labeled plasmid pBR322 DNA as a control probe. 9k-Da CaBP mRNA, visualized by silver grains, was found only in the absorptive epithelial cells, and the concentration was greater in the cells at the villous tips than in those of the crypts. The 9k-Da CaBP mRNA was observed mainly in the cytoplasm of the columnar cells and less frequently in the nucleus. Labeling was not seen in the brush border and goblet cells. The submucosa, with Brunner's glands and muscularis, also showed no specific 9-kDa CaBP mRNA concentration. This demonstration of 9-kDa CaBP gene activity in the columnar cells of the rat duodenum illustrates the usefulness of in situ hybridization for characterization of specific cells involved in the expression of 1,25(OH)2 D3 activity.

Amino acid sequence of the sex steroid binding protein of human blood plasma

Biochemistry ◽  
1986 ◽  
Vol 25 (23) ◽  
pp. 7584-7590 ◽  
Author(s):  
Kenneth A. Walsh ◽  
Koiti Titani ◽  
Koji Takio ◽  
Santosh Kumar ◽  
Rutherford Hayes ◽  
...  
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Blood Plasma ◽  
Human Blood ◽  
Binding Protein ◽  
Sex Steroid ◽  
Human Blood Plasma ◽  
Steroid Binding ◽  
Steroid Binding Protein
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