Cloning and disruption of Ustilago maydis genes

1989 ◽  
Vol 9 (9) ◽  
pp. 4052-4055
Author(s):  
S Fotheringham ◽  
W K Holloman
Keyword(s):  
High Frequency ◽  
Ustilago Maydis ◽  
Amino Acid Biosynthesis ◽  
Acid Biosynthesis ◽  
Genomic Libraries ◽  
Transformation Vector ◽  
General Utility ◽  
One Step ◽  
High Frequency Transformation ◽  
Made In

We have demonstrated that genes from Ustilago maydis can be cloned by direct complementation of mutants through the use of genomic libraries made in a high-frequency transformation vector. We isolated a gene involved in amino acid biosynthesis as an illustrative example and showed that integrative and one-step disruption methods can be used to create null mutations in the chromosomal copy of the gene by homologous recombination. The results of this investigation make it clear that one-step gene disruption will be of general utility in investigations of U. maydis, since simple, precise replacement of the sequence under study was readily achieved.

scholarly journals Cloning and disruption of Ustilago maydis genes.

1989 ◽  
Vol 9 (9) ◽  
pp. 4052-4055 ◽  
Author(s):  
S Fotheringham ◽  
W K Holloman
Keyword(s):  
High Frequency ◽  
Ustilago Maydis ◽  
Amino Acid Biosynthesis ◽  
Acid Biosynthesis ◽  
Genomic Libraries ◽  
Transformation Vector ◽  
General Utility ◽  
One Step ◽  
High Frequency Transformation ◽  
Made In

We have demonstrated that genes from Ustilago maydis can be cloned by direct complementation of mutants through the use of genomic libraries made in a high-frequency transformation vector. We isolated a gene involved in amino acid biosynthesis as an illustrative example and showed that integrative and one-step disruption methods can be used to create null mutations in the chromosomal copy of the gene by homologous recombination. The results of this investigation make it clear that one-step gene disruption will be of general utility in investigations of U. maydis, since simple, precise replacement of the sequence under study was readily achieved.

Structure of the yeast HIS5 gene responsive to general control of amino acid biosynthesis

1987 ◽  
Vol 208 (1-2) ◽  
pp. 159-167 ◽  
Author(s):  
Kiyoji Nishiwaki ◽  
Naoyuki Hayashi ◽  
Shinji Irie ◽  
Dong-Hyo Chung ◽  
Satoshi Harashima ◽  
...  
Keyword(s):  
Amino Acid ◽  
Amino Acid Biosynthesis ◽  
General Control ◽  
Acid Biosynthesis

Manufacturing Processes of Solid Oxide Fuel Cell Components

2007 ◽  
Vol 336-338 ◽  
pp. 498-501
Author(s):  
Xian Feng Jiang ◽  
Min Fang Han ◽  
Su Ping Peng
Keyword(s):  
Fuel Cell ◽  
Solid Oxide Fuel Cell ◽  
Screen Printing ◽  
Sol Gel ◽  
Tape Casting ◽  
Solid Oxide ◽  
Oxide Fuel ◽  
Cell Components ◽  
One Step ◽  
Made In

The all processes for manufacturing materials parts of solid oxide fuel cell (SOFC) are discussed in the paper. The films are made in one step by the ways of APS, VPS, EVD, which are usually used to produce the electrolyte and interconnect. The films are thin and good gas-resistance, but with relatively high cost. All parts of SOFC are made by the following ways, such as sol-gel, tape casting, tape calendaring and screen printing, which are suitable for manufacturing samples in industry with the cheapest process by co-sintered together ways.

scholarly journals Incorporation of alternative amino acids into cyanophycin by different cyanophycin synthetases heterologously expressed in Corynebacterium glutamicum

AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ramona Wördemann ◽  
Lars Wiefel ◽  
Volker F. Wendisch ◽  
Alexander Steinbüchel
Keyword(s):  
Amino Acids ◽  
Dry Weight ◽  
Growth Conditions ◽  
Amino Acid Biosynthesis ◽  
Water Soluble ◽  
Lysine Content ◽  
Polyaspartic Acid ◽  
Acid Biosynthesis ◽  
Cyanophycin Synthetase ◽  
Potential Precursor

AbstractCyanophycin (multi-l-arginyl-poly-l-aspartic acid; also known as cyanophycin grana peptide [CGP]) is a biopolymer that could be used in various fields, for example, as a potential precursor for the synthesis of polyaspartic acid or for the production of CGP-derived dipeptides. To extend the applications of this polymer, it is therefore of interest to synthesize CGP with different compositions. A recent re-evaluation of the CGP synthesis in C. glutamicum has shown that C. glutamicum is a potentially interesting microorganism for CGP synthesis with a high content of alternative amino acids. This study shows that the amount of alternative amino acids can be increased by using mutants of C. glutamicum with altered amino acid biosynthesis. With the DM1729 mutant, the lysine content in the polymer could be increased up to 33.5 mol%. Furthermore, an ornithine content of up to 12.6 mol% was achieved with ORN2(Pgdh4). How much water-soluble or insoluble CGP is synthesized is strongly related to the used cyanophycin synthetase. CphADh synthesizes soluble CGP exclusively. However, soluble CGP could also be isolated from cells expressing CphA6308Δ1 or CphA6308Δ1_C595S in addition to insoluble CGP in all examined strains. The point mutation in CphA6308Δ1_C595S partially resulted in a higher lysine content. In addition, the CGP content could be increased to 36% of the cell dry weight under optimizing growth conditions in C. glutamicum ATCC13032. All known alternative major amino acids for CGP synthesis (lysine, ornithine, citrulline, and glutamic acid) could be incorporated into CGP in C. glutamicum.

scholarly journals Transfer RNA-dependent amino acid biosynthesis: An essential route to asparagine formation

2002 ◽  
Vol 99 (5) ◽  
pp. 2678-2683 ◽  
Author(s):  
B. Min ◽  
J. T. Pelaschier ◽  
D. E. Graham ◽  
D. Tumbula-Hansen ◽  
D. Soll
Keyword(s):  
Amino Acid ◽  
Amino Acid Biosynthesis ◽  
Transfer Rna ◽  
Acid Biosynthesis

One Step Forward, Two Steps Back

2014 ◽  
Vol 10 (1) ◽  
pp. 1-15 ◽  
Author(s):  
Stephan Engelkamp ◽  
Katharina Glaab ◽  
Judith Renner
Keyword(s):  
Critical Approach ◽  
Critical Potential ◽  
Specific Reading ◽  
Normative Evaluation ◽  
One Step ◽  
Made In

AbstractIn their response to our article »Office Hours«, Nicole Deitelhoff and Lisbeth Zimmermann issue three major points of critique towards our proposal of a critical approach to norm research: They criticize, firstly, our discussion of constructivist norm research, secondly, our use of the concepts of local and Western and, thirdly, the overall critical potential of our proposed approach, which they criticize as going merely beyond an unmasking gesture. We take our response to our critics, firstly, as an opportunity to clarify some of the arguments made in our article. Secondly, we confront the points of criticism outlined above and show that Deitelhoff’s and Zimmermann’s critique can only be maintained if one accepts their specific reading of our article. Moreover, it gets tangled up in three major contradictions and is built upon a problematic understanding of the relation between empirical facticity and normative evaluation.

Sign in / Sign up

Export Citation Format

Share Document