scholarly journals Whole-Genome Sequencing and Annotation of 10 Endophytic and Epiphytic Bacteria Isolated from Lolium arundinaceum

2021 ◽  
Vol 10 (19) ◽  
Author(s):  
Victoria A. Gaeth ◽  
Christina J. Domondon ◽  
Paul A. Podbielski ◽  
Virginia X. Aswad ◽  
Emalee A. Wrightstone ◽  
...  
Keyword(s):  
New York ◽  
Whole Genome Sequence ◽  
Whole Genome ◽  
Epiphytic Bacteria ◽  
Lolium Arundinaceum ◽  
Content Type ◽  
Laboratory Exercise ◽  
Rochester Institute Of Technology ◽  
Plant Biochemistry ◽  
Institute Of Technology

We report the whole-genome sequence and annotation of 10 endophytic and epiphytic bacteria isolated from the grass Lolium arundinaceum as part of a laboratory exercise in a Fundamentals of Plant Biochemistry and Pathology undergraduate course (BIOL403) at the Rochester Institute of Technology in Rochester, New York.

scholarly journals Isolation and Whole-Genome Sequencing of 12 Mushroom-Associated Bacterial Strains: an Inquiry-Based Laboratory Exercise in a Genomics Course at the Rochester Institute of Technology

2020 ◽  
Vol 9 (6) ◽  
Author(s):  
Narayan H. Wong ◽  
Andrew J. Rosato ◽  
Yara M. Rose ◽  
Trevor S. Penix ◽  
Janice B. Fung ◽  
...  
Keyword(s):  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Life Sciences ◽  
Whole Genome ◽  
Bacterial Strains ◽  
Genome Sequences ◽  
Mushroom Species ◽  
Laboratory Exercise ◽  
Rochester Institute Of Technology ◽  
Institute Of Technology

Here, we report the isolation, identification, and whole-genome sequences of 12 bacterial strains associated with four mushroom species. The study was done as an inquiry-based exercise in an undergraduate genomics course (BIOL 340) in the Thomas H. Gosnell School of Life Sciences at the Rochester Institute of Technology.

scholarly journals Whole-Genome Sequencing and Annotation of Exiguobacterium sp. RIT 452, an Antibiotic-Producing Strain Isolated from a Pond Located on the Campus of the Rochester Institute of Technology

2018 ◽  
Vol 7 (17) ◽  
Author(s):  
Anutthaman Parthasarathy ◽  
Narayan H. Wong ◽  
Nicole T. Cavanaugh ◽  
KayLee K. Steiner ◽  
Peter C. Wengert ◽  
...  
Keyword(s):  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Antibiotic Production ◽  
Whole Genome ◽  
Content Type ◽  
Rochester Institute Of Technology ◽  
Institute Of Technology

Exiguobacterium sp. RIT 452 is of biotechnological importance given its potential for antibiotic production.

scholarly journals Complete Genome Sequence of the Arcobacter skirrowii Type Strain LMG 6621

2018 ◽  
Vol 7 (17) ◽  
Author(s):  
William G. Miller ◽  
Emma Yee
Keyword(s):  
Genome Sequence ◽  
Fecal Sample ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Type Strain ◽  
Whole Genome Sequence ◽  
Whole Genome ◽  
Content Type ◽  
The United Kingdom ◽  
Veterinary Importance

Arcobacter skirrowii is a species of veterinary importance, originally recovered from the feces, aborted fetuses, and preputial fluids of livestock. We present here the whole-genome sequence of the A. skirrowii type strain LMG 6621 (= 449/80T = CCUG 10374T), isolated in the United Kingdom from a lamb diarrheal fecal sample.

scholarly journals Whole-Genome Sequence of Salmonella enterica subsp. enterica Serovar Typhimurium Strain UPM 260, Isolated from a Broiler Chicken in Perak, Malaysia

2017 ◽  
Vol 5 (46) ◽  
Author(s):  
Najwa Syahirah Roslan ◽  
Shagufta Jabeen ◽  
Nurulfiza Mat Isa ◽  
Abdul Rahman Omar ◽  
Mohd Hair Bejo ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Salmonella Enterica ◽  
Broiler Chicken ◽  
Whole Genome Sequence ◽  
Whole Genome ◽  
Content Type ◽  
Typhimurium Strain ◽  
Serovar Typhimurium

ABSTRACT Salmonella enterica subsp. enterica serovar Typhimurium is one of several well-categorized Salmonella serotypes recognized globally. Here, we report the whole-genome sequence of S. Typhimurium strain UPM 260, isolated from a broiler chicken.

scholarly journals Whole-Genome Sequence of Lactobacillus salivarius DJ-sa-01, Isolated from Chicken Small Intestine

2018 ◽  
Vol 7 (14) ◽  
Author(s):  
Dongjun Kim ◽  
Mun-Ju Cho ◽  
Seungchan Cho ◽  
Yongjun Lee ◽  
Sung June Byun ◽  
...  
Keyword(s):  
Small Intestine ◽  
Genome Sequence ◽  
Probiotic Strain ◽  
Whole Genome Sequence ◽  
Whole Genome ◽  
Lactobacillus Salivarius ◽  
Circular Chromosome ◽  
Content Type ◽  
Single Circular Chromosome

We have identified the whole-genome sequence of Lactobacillus salivarius DJ-sa-01, a potential probiotic strain for poultry, isolated from a chicken small intestine. We used the PacBio and Illumina platforms to obtain the sequence of the entire single circular chromosome.

scholarly journals Complete Genome Sequence of Streptococcus oralis SF100, Isolated from Blood Cultures from a Patient with Infective Endocarditis

2021 ◽  
Vol 10 (28) ◽  
Author(s):  
Ji Hee Lee ◽  
Hyun Jung Ji ◽  
Ho Seong Seo ◽  
Paul M. Sullam
Keyword(s):  
Infective Endocarditis ◽  
Animal Models ◽  
Oral Cavity ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Blood Cultures ◽  
Whole Genome Sequence ◽  
Whole Genome ◽  
Content Type ◽  
Streptococcus Oralis

Streptococcus oralis is a commensal viridans group streptococcus of the human oral cavity and a frequent cause of endovascular infection. Here, we report the complete whole-genome sequence of S. oralis strain SF100, which was originally isolated from the blood of a patient with infective endocarditis. This strain contains the lysogenic bacteriophage SM1, which enhances the virulence of SF100 in animal models of endocardial infection.

scholarly journals Draft Genome Sequence of Acholeplasma laidlawii Isolated from the Conjunctiva of a Heifer with Infectious Bovine Keratoconjunctivitis

2021 ◽  
Vol 10 (4) ◽  
Author(s):  
Gloria Gioia ◽  
Maria F. Addis ◽  
Laura B. Goodman ◽  
Patrick K. Mitchell ◽  
Belinda Thompson ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Whole Genome Sequence ◽  
Whole Genome ◽  
Content Type ◽  
Infectious Bovine Keratoconjunctivitis ◽  
Acholeplasma Laidlawii

ABSTRACT Acholeplasma laidlawii can be isolated from cattle environments and different body sites of bovines. It is still under evaluation if A. laidlawii acts as a primary pathogen. Here, we present the whole-genome sequence of A. laidlawii isolated from the conjunctiva of a heifer with infectious bovine keratoconjunctivitis.

scholarly journals Finished Whole-Genome Sequence of Clostridium argentinense Producing Botulinum Neurotoxin Type G

2017 ◽  
Vol 5 (21) ◽  
Author(s):  
Jessica L. Halpin ◽  
Karen Hill ◽  
Shannon L. Johnson ◽  
David Carlton Bruce ◽  
T. Brian Shirey ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Botulinum Neurotoxin ◽  
Whole Genome Sequence ◽  
Whole Genome ◽  
Content Type ◽  
Reference Quality

ABSTRACT Here, we present a closed genome sequence for Clostridium argentinense strain 89G, the first strain identified to produce botulinum neurotoxin type G (BoNT/G). Although discovered in 1970, to date, there have been no reference quality sequences publicly available for this species.

scholarly journals Whole-Genome Sequencing Allows for Improved Identification of Persistent Listeria monocytogenes in Food-Associated Environments

2015 ◽  
Vol 81 (17) ◽  
pp. 6024-6037 ◽  
Author(s):  
Matthew J. Stasiewicz ◽  
Haley F. Oliver ◽  
Martin Wiedmann ◽  
Henk C. den Bakker
Keyword(s):  
Listeria Monocytogenes ◽  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Whole Genome Sequence ◽  
Whole Genome ◽  
Genetic Determinants ◽  
Single Nucleotide ◽  
Content Type ◽  
Food Borne ◽  
Clonal Spread

ABSTRACTWhile the food-borne pathogenListeria monocytogenescan persist in food associated environments, there are no whole-genome sequence (WGS) based methods to differentiate persistent from sporadic strains. Whole-genome sequencing of 188 isolates from a longitudinal study ofL. monocytogenesin retail delis was used to (i) apply single-nucleotide polymorphism (SNP)-based phylogenetics for subtyping ofL. monocytogenes, (ii) use SNP counts to differentiate persistent from repeatedly reintroduced strains, and (iii) identify genetic determinants ofL. monocytogenespersistence. WGS analysis revealed three prophage regions that explained differences between three pairs of phylogenetically similar populations with pulsed-field gel electrophoresis types that differed by ≤3 bands. WGS-SNP-based phylogenetics found that putatively persistentL. monocytogenesrepresent SNP patterns (i) unique to a single retail deli, supporting persistence within the deli (11 clades), (ii) unique to a single state, supporting clonal spread within a state (7 clades), or (iii) spanning multiple states (5 clades). Isolates that formed one of 11 deli-specific clades differed by a median of 10 SNPs or fewer. Isolates from 12 putative persistence events had significantly fewer SNPs (median, 2 to 22 SNPs) than between isolates of the same subtype from other delis (median up to 77 SNPs), supporting persistence of the strain. In 13 events, nearly indistinguishable isolates (0 to 1 SNP) were found across multiple delis. No individual genes were enriched among persistent isolates compared to sporadic isolates. Our data show that WGS analysis improves food-borne pathogen subtyping and identification of persistent bacterial pathogens in food associated environments.

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