scholarly journals Complete Genome Sequences of Mycobacterium Phages Tripl3t and Zeuska

2021 ◽  
Vol 10 (32) ◽  
Author(s):  
Faith Cox ◽  
Tiffany Lujan ◽  
Matthew Bristerpostma ◽  
Rheaven Sandoval ◽  
Haze Murphy ◽  
...  

Tripl3t and Zeuska are siphoviral bacteriophages that were isolated from Mycobacterium smegmatis mc 2 155 and contain double-stranded DNA genomes 53,565 bp and 53,598 bp in length, respectively. Tripl3t and Zeuska were annotated by students at Bluff Dale High School (Bluff Dale, TX) and Tolar High School (Tolar, TX) in community engagement with Tarleton State University.

2020 ◽  
Vol 9 (45) ◽  
Author(s):  
Denzel Edwards ◽  
Tara Carney ◽  
Yunia Alvarez ◽  
Justin Pagan ◽  
Emma Sarro ◽  
...  

ABSTRACT Apex and Gophee are mycobacteriophages directly isolated from soil using the host Mycobacterium smegmatis mc2155. Apex has a 71,244-bp double-stranded DNA (dsDNA) genome encoding 98 putative proteins, and Gophee has a 68,556-bp dsDNA genome encoding 101 putative proteins.


2018 ◽  
Vol 6 (21) ◽  
Author(s):  
Nikolay V. Volozhantsev ◽  
Egor A. Denisenko ◽  
Angelina A. Kislichkina ◽  
Vera P. Myakinina ◽  
Valentina M. Krasilnikova ◽  
...  

ABSTRACT Two lytic double-stranded DNA bacteriophages, VSe11 and VSe102, infecting broad-spectrum Salmonella enterica were isolated from the sewage of two different poultry farms. The phage genomes comprise 86,360 bp and 86,365 bp, respectively, with a G+C content of 39.0%, and both contain 129 putative coding sequences.


2020 ◽  
Vol 9 (33) ◽  
Author(s):  
Kayla M. Fast ◽  
Kadaisha G. Johnson ◽  
Kaitlyn N. Mayfield ◽  
Leah A. Stephens ◽  
T. Hunter Reid ◽  
...  

ABSTRACT Mycobacteriophages OKaNui and DroogsArmy were isolated from soil using the bacterial host Mycobacterium smegmatis mc2155, which belongs to the phylum Actinobacteria. OKaNui was discovered in east Mississippi and DroogsArmy in west Alabama in the United States. The genomes of OKaNui and DroogsArmy were 51,424 bp and 53,254 bp long, respectively.


2020 ◽  
Vol 9 (3) ◽  
Author(s):  
John Grossi ◽  
John Shebby ◽  
Christopher W. Brey

Mycobacteriophage Faze9 is a novel lytic phage that was isolated from soil collected in Scranton, PA, using the host Mycobacterium smegmatis mc2155. Faze9 has a double-stranded DNA genome composed of 67,503 kbp, has a G+C content of 68.9%, encodes 91 predicted proteins, and is closely related to mycobacteriophages in the B2 subcluster. 


2018 ◽  
Vol 6 (27) ◽  
Author(s):  
Hue Thi Kim Vu ◽  
Matthew J. Stasiewicz ◽  
Soottawat Benjakul ◽  
Kitiya Vongkamjan

We report here the complete genome sequences of three Listeria phages (PSU-VKH-LP019, PSU-VKH-LP040, and PSU-VKH-LP041), which were newly induced from lysogenic isolates of Listeria monocytogenes from seafood and a seafood processing environment in Thailand. The three phages show circularly permuted double-stranded DNA genomes with sizes of 38.6, 39.6, and 48.3 kb.


2019 ◽  
Vol 8 (30) ◽  
Author(s):  
Kayla M. Fast ◽  
Brianna E. Forrest ◽  
Garren R. Granec ◽  
John D. Larrimore ◽  
Anna E. Morse ◽  
...  

Mycobacteriophages Candle, Schatzie, Sumter, and Waleliano were isolated from soil using the host bacterium Mycobacterium smegmatis mc2155. Candle, Schatzie, and Sumter were discovered in Alabama and Waleliano in Maryland. The bacteriophages have been assigned clusters based on nucleotide similarity, as follows: Candle, R; Schatzie, J; Sumter, A1; and Waleliano, B4.


2017 ◽  
Vol 5 (29) ◽  
Author(s):  
Ivan Erill ◽  
Steven M. Caruso

ABSTRACT Three double-stranded DNA phi29-like Bacillus cereus group bacteriophages, BeachBum, Harambe, and SerPounce, were identified and characterized. BeachBum and Harambe are closely related but are remarkably different from previously identified phi29-like phages. SerPounce is substantially closer to other phi29-like phages, enabling the identification of its prohead RNA (pRNA) gene.


2017 ◽  
Vol 5 (43) ◽  
Author(s):  
Kristen A. Butela ◽  
Susan M. R. Gurney ◽  
Heather L. Hendrickson ◽  
Janine M. LeBlanc-Straceski ◽  
Anastasia M. Zimmerman ◽  
...  

ABSTRACT Seven mycobacteriophages from distinct geographical locations were isolated, using Mycobacterium smegmatis mc2155 as the host, and then purified and sequenced. All of the genomes are related to cluster A mycobacteriophages, BobSwaget and Lokk in subcluster A2; Fred313, KADY, Stagni, and StepMih in subcluster A3; and MyraDee in subcluster A18, the first phage to be assigned to that subcluster.


2018 ◽  
Vol 7 (12) ◽  
Author(s):  
Devin Blocker ◽  
Matthew Koert ◽  
Courtney Mattson ◽  
Harsh Patel ◽  
Priya Patel ◽  
...  

Six double-stranded DNA Streptomyces bacteriophages, HotFries, Moozy, RavenPuff, Scap1, Rainydai, and SenditCS, were isolated using the phytopathogen Streptomyces scabiei as a host. These phages have been identified as Siphoviridae and members of cluster BI by genomic analysis.


2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Darlenys Sanchez ◽  
Anna Acosta ◽  
Nicole Skoumpourdis ◽  
Regina Alvarez ◽  
Bernadette J. Connors

ABSTRACT Mycobacterium smegmatis, an acid-fast bacterial species in the phylum Actinobacteria, has often been used as a substitute for pathogenic mycobacteria in research. Here, we describe the isolation and characterization of two M. smegmatis bacteriophages, Penelope2018 and Miniwave.


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