scholarly journals Complete Genome Sequences of Two Mycobacterium smegmatis Bacteriophages

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Darlenys Sanchez ◽  
Anna Acosta ◽  
Nicole Skoumpourdis ◽  
Regina Alvarez ◽  
Bernadette J. Connors
Keyword(s):  
Mycobacterium Smegmatis ◽  
Complete Genome ◽  
Bacterial Species ◽  
Genome Sequences ◽  
Content Type ◽  
Isolation And Characterization

ABSTRACT Mycobacterium smegmatis, an acid-fast bacterial species in the phylum Actinobacteria, has often been used as a substitute for pathogenic mycobacteria in research. Here, we describe the isolation and characterization of two M. smegmatis bacteriophages, Penelope2018 and Miniwave.

scholarly journals Complete Genome Sequences of Two Vibrio natriegens Bacteriophages

2020 ◽  
Vol 9 (45) ◽  
Author(s):  
Meghan T. Harris ◽  
Tereasa Ching Ho ◽  
Harry Fruchtman ◽  
Mira E. Garin ◽  
Victor Kubatin ◽  
...  
Keyword(s):  
Metabolic Engineering ◽  
Complete Genome ◽  
Negative Bacterium ◽  
Genome Sequences ◽  
Gram Negative ◽  
Content Type ◽  
Fast Growing ◽  
Isolation And Characterization

ABSTRACT Vibrio natriegens, a fast-growing Gram-negative bacterium, is gaining interest as a platform for rapid biotechnology applications and metabolic engineering. Only a few bacteriophages that infect this bacterium have been identified. Here, we describe the isolation and characterization of two V. natriegens bacteriophages isolated from Hatches Creek, Wellfleet, Massachusetts.

scholarly journals Complete Genome Sequences of Mycobacteriophages OKaNui and DroogsArmy

2020 ◽  
Vol 9 (33) ◽  
Author(s):  
Kayla M. Fast ◽  
Kadaisha G. Johnson ◽  
Kaitlyn N. Mayfield ◽  
Leah A. Stephens ◽  
T. Hunter Reid ◽  
...  
Keyword(s):  
United States ◽  
Mycobacterium Smegmatis ◽  
Complete Genome ◽  
The United States ◽  
Genome Sequences ◽  
Bacterial Host ◽  
Content Type

ABSTRACT Mycobacteriophages OKaNui and DroogsArmy were isolated from soil using the bacterial host Mycobacterium smegmatis mc2155, which belongs to the phylum Actinobacteria. OKaNui was discovered in east Mississippi and DroogsArmy in west Alabama in the United States. The genomes of OKaNui and DroogsArmy were 51,424 bp and 53,254 bp long, respectively.

scholarly journals Complete Genome Sequences of Mycobacteriophages Candle, Schatzie, Sumter, and Waleliano

2019 ◽  
Vol 8 (30) ◽  
Author(s):  
Kayla M. Fast ◽  
Brianna E. Forrest ◽  
Garren R. Granec ◽  
John D. Larrimore ◽  
Anna E. Morse ◽  
...  
Keyword(s):  
Mycobacterium Smegmatis ◽  
Complete Genome ◽  
Host Bacterium ◽  
Genome Sequences ◽  
Content Type

Mycobacteriophages Candle, Schatzie, Sumter, and Waleliano were isolated from soil using the host bacterium Mycobacterium smegmatis mc2155. Candle, Schatzie, and Sumter were discovered in Alabama and Waleliano in Maryland. The bacteriophages have been assigned clusters based on nucleotide similarity, as follows: Candle, R; Schatzie, J; Sumter, A1; and Waleliano, B4.

scholarly journals Complete Genome Sequences of Clinical Pandoraea fibrosis Isolates

2020 ◽  
Vol 9 (13) ◽  
Author(s):  
Miranda E. Pitt ◽  
Son H. Nguyen ◽  
Tânia P. S. Duarte ◽  
Louise F. Roddam ◽  
Mark A. T. Blaskovich ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Respiratory Tract ◽  
Cystic Fibrosis Patient ◽  
Antibiotic Treatment ◽  
Complete Genome ◽  
Bacterial Species ◽  
Genome Sequences ◽  
Gram Negative ◽  
Content Type

Pandoraea fibrosis is a newly identified Gram-negative bacterial species that was isolated from the respiratory tract of an Australian cystic fibrosis patient. The complete assembled genome sequences of two consecutive isolates (second isolate collected 11 months after antibiotic treatment) from the same individual are presented here.

scholarly journals Whole-Genome Sequences of Salmonella enterica subsp. enterica Serovar Typhimurium Strains TT6675 and TT9097 Employed in the Isolation and Characterization of a Giant Phage Mutant Collection

2017 ◽  
Vol 5 (34) ◽  
Author(s):  
Han Ming Gan ◽  
Wilhelm Wei Han Eng ◽  
Melissa K. Barton ◽  
Lily E. Adams ◽  
Nurul Aisyah Samsudin ◽  
...  
Keyword(s):  
Salmonella Enterica ◽  
Whole Genome ◽  
Genome Sequences ◽  
Content Type ◽  
Phage Mutant ◽  
Isolation And Characterization ◽  
Serovar Typhimurium ◽  
Bacterial Viruses ◽  
Mutant Collection

ABSTRACT We report here the genome sequences of Salmonella enterica subsp. enterica serovar Typhimurium strains TT6675 and TT9097, which we utilize for genetic analyses of giant bacterial viruses. Our analyses identified several genetic variations between the two strains, most significantly confirming strain TT6675 as a serine suppressor and TT9097 as a nonsuppressor.

scholarly journals Complete Genome Sequence of Klebsiella pneumoniae Myophage Mineola

2019 ◽  
Vol 8 (17) ◽  
Author(s):  
Justin X. Boeckman ◽  
Lauren Lessor ◽  
Jason J. Gill ◽  
Mei Liu
Keyword(s):  
Drug Resistance ◽  
Klebsiella Pneumoniae ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Human Pathogen ◽  
Content Type ◽  
Isolation And Characterization ◽  
Wide Range

Klebsiella pneumoniae is an important human pathogen due to the wide range of infections it can cause and its emerging drug resistance. Isolation and characterization of phage infecting K. pneumoniae could be important for future therapeutic applications.

scholarly journals Characterization of a Diffusible Signaling Factor from Xylella fastidiosa

mBio ◽  
2013 ◽  
Vol 4 (1) ◽  
Author(s):  
Ellen D. Beaulieu ◽  
Michael Ionescu ◽  
Subhadeep Chatterjee ◽  
Kenji Yokota ◽  
Dirk Trauner ◽  
...  
Keyword(s):  
Cell Signaling ◽  
Unsaturated Fatty Acids ◽  
Xylella Fastidiosa ◽  
Bacterial Species ◽  
Content Type ◽  
Isolation And Characterization ◽  
High Level ◽  
Diffusible Signaling Factor ◽  
Cell Cell

ABSTRACTCell-cell signaling inXylella fastidiosahas been implicated in the coordination of traits enabling colonization in plant hosts as well as insect vectors. This cell density-dependent signaling has been attributed to a diffusible signaling factor (DSF) produced by the DSF synthase RpfF. DSF produced by related bacterial species are unsaturated fatty acids, but that ofX. fastidiosawas thought to be different from those of other taxa. We describe here the isolation and characterization of anX. fastidiosaDSF (XfDSF) as 2(Z)-tetradecenoic acid. This compound was isolated both from recombinantErwinia herbicolaexpressingX. fastidiosa rpfFand from anX. fastidiosa rpfCdeletion mutant that overproduces DSF. Since anrpfFmutant is impaired in biofilm formation and underexpresses the hemagglutinin-like protein-encoding geneshxfAandhxfB, we demonstrate that these traits can be restored by ca. 0.5 µMXfDSF but not by myristic acid, the fully saturated tetradecenoic acid. AphoA-basedX. fastidiosabiosensor that assesses DSF-dependent expression ofhxfAorhxfBrevealed a high level of molecular specificity of DSF signaling.IMPORTANCEX. fastidiosacauses diseases in many important plants, including grape, where it incites Pierce’s disease. Virulence ofX. fastidiosafor grape is coordinated by cell-cell signaling molecules, designated DSF (Diffusible Signaling Factor). Mutants blocked in DSF production are hypervirulent for grape, suggesting that virulence is suppressed upon DSF accumulation and that disease could be controlled by artificial elevation of the DSF level in plants. In this work, we describe the isolation of the DSF produced byX. fastidiosaand the verification of its biological activity as an antivirulence factor. We also have developedX. fastidiosaDSF biosensors to evaluate the specificity of cell-cell signaling to be investigated.

scholarly journals Complete Genome Sequence of Klebsiella pneumoniae Podophage Pylas

2019 ◽  
Vol 8 (46) ◽  
Author(s):  
Jeffery E. Powell ◽  
Lauren Lessor ◽  
Chandler O’Leary ◽  
Jason Gill ◽  
Mei Liu
Keyword(s):  
Drug Resistance ◽  
Klebsiella Pneumoniae ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Opportunistic Pathogen ◽  
Content Type ◽  
Isolation And Characterization

Carbapenemase-producing Klebsiella pneumoniae is an important opportunistic pathogen due to its drug resistance. This study reports on the isolation and characterization of a podophage, named Pylas, infecting this bacterium. The complete genome of phage Pylas is described, and it is distantly related to the well-studied phage N4.

scholarly journals Complete Genome Sequences of Cluster A Mycobacteriophages BobSwaget, Fred313, KADY, Lokk, MyraDee, Stagni, and StepMih

2017 ◽  
Vol 5 (43) ◽  
Author(s):  
Kristen A. Butela ◽  
Susan M. R. Gurney ◽  
Heather L. Hendrickson ◽  
Janine M. LeBlanc-Straceski ◽  
Anastasia M. Zimmerman ◽  
...  
Keyword(s):  
Mycobacterium Smegmatis ◽  
Complete Genome ◽  
Genome Sequences ◽  
Content Type ◽  
Cluster A ◽  
Geographical Locations

ABSTRACT Seven mycobacteriophages from distinct geographical locations were isolated, using Mycobacterium smegmatis mc2155 as the host, and then purified and sequenced. All of the genomes are related to cluster A mycobacteriophages, BobSwaget and Lokk in subcluster A2; Fred313, KADY, Stagni, and StepMih in subcluster A3; and MyraDee in subcluster A18, the first phage to be assigned to that subcluster.

scholarly journals Complete Genome Sequences of Mycobacteriophages Apex and Gophee

2020 ◽  
Vol 9 (45) ◽  
Author(s):  
Denzel Edwards ◽  
Tara Carney ◽  
Yunia Alvarez ◽  
Justin Pagan ◽  
Emma Sarro ◽  
...  
Keyword(s):  
Mycobacterium Smegmatis ◽  
Complete Genome ◽  
Genome Sequences ◽  
Content Type ◽  
Double Stranded Dna

ABSTRACT Apex and Gophee are mycobacteriophages directly isolated from soil using the host Mycobacterium smegmatis mc2155. Apex has a 71,244-bp double-stranded DNA (dsDNA) genome encoding 98 putative proteins, and Gophee has a 68,556-bp dsDNA genome encoding 101 putative proteins.

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