scholarly journals Genome Sequences of Allochromatium palmeri and Allochromatium humboldtianum Expand the Allochromatium Family Tree of Purple Sulfur Photosynthetic Bacteria within the Gammaproteobacteria and Further Refine the Genus

2020 ◽  
Vol 9 (33) ◽  
Author(s):  
John A. Kyndt ◽  
Terry E. Meyer
Keyword(s):  
Photosynthetic Bacteria ◽  
Nucleotide Identity ◽  
Whole Genome ◽  
Family Tree ◽  
Average Nucleotide Identity ◽  
Genome Sequences ◽  
Allochromatium Vinosum ◽  
Content Type ◽  
The Family ◽  
Identity Based

ABSTRACT New genomes of two Allochromatium strains were sequenced. Whole-genome and average nucleotide identity based on BLAST (ANIb) comparisons show that Allochromatium humboldtianum is the nearest relative of Allochromatium vinosum (ANIb, 91.5%), while both Allochromatium palmeri and Thermochromatium tepidum are more distantly related (ANIb, <87%). These new sequences firmly establish the position of Allochromatium on the family tree.

scholarly journals Draft Genome Sequences of Thiorhodococcus mannitoliphagus and Thiorhodococcus minor, Purple Sulfur Photosynthetic Bacteria in the Gammaproteobacterial Family Chromatiaceae

2020 ◽  
Vol 9 (14) ◽  
Author(s):  
Fabiola A. Aviles ◽  
Terry E. Meyer ◽  
John A. Kyndt
Keyword(s):  
Photosynthetic Bacteria ◽  
Draft Genome ◽  
Nucleotide Identity ◽  
Whole Genome ◽  
Average Nucleotide Identity ◽  
Genome Sequences ◽  
Content Type

We have determined the draft genome sequences of Thiorhodococcus mannitoliphagus and Thiorhodococcus minor for comparison with those of T. drewsii and Imhoffiella purpurea. According to average nucleotide identity (ANI) and whole-genome phylogenetic comparisons, these two species are clearly distinct from the Imhoffiella species and T. drewsii.

scholarly journals Whole-Genome Sequences of Two Carbapenem-Resistant Klebsiella quasipneumoniae Strains Isolated from a Tertiary Hospital in Johor, Malaysia

2017 ◽  
Vol 5 (32) ◽  
Author(s):  
Han Ming Gan ◽  
Ganeswrie Rajasekaram ◽  
Wilhelm Wei Han Eng ◽  
Priyatharisni Kaniappan ◽  
Amreeta Dhanoa
Keyword(s):  
Tertiary Hospital ◽  
Clinical Isolates ◽  
Nucleotide Identity ◽  
Whole Genome ◽  
Average Nucleotide Identity ◽  
Genome Sequences ◽  
Content Type ◽  
Carbapenem Resistant ◽  
Extended Spectrum

ABSTRACT We report the whole-genome sequences of two carbapenem-resistant clinical isolates of Klebsiella quasipneumoniae subsp. similipneumoniae obtained from two different patients. Both strains contained three different extended-spectrum β-lactamase genes and showed strikingly high pairwise average nucleotide identity of 99.99% despite being isolated 3 years apart from the same hospital.

scholarly journals Complete Genome Sequences of Thermus thermophilus Strains HB5002 and HB5008, Isolated from Mine Hot Spring in Japan

2021 ◽  
Vol 10 (16) ◽  
Author(s):  
Kentaro Miyazaki ◽  
Toshiyuki Moriya ◽  
Natsuko Tokito ◽  
Tairo Oshima ◽  
Kei Yura ◽  
...  
Keyword(s):  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Complete Genome ◽  
Hot Spring ◽  
Thermus Thermophilus ◽  
Nucleotide Identity ◽  
Whole Genome ◽  
Average Nucleotide Identity ◽  
Genome Sequences ◽  
Content Type

ABSTRACT We isolated Thermus thermophilus strains HB5002 and HB5008 from Mine Hot Spring in Japan. Whole-genome sequencing revealed that they showed ∼100% average nucleotide identity to each other, ≥98.53% to the T. thermophilus strains originating from the same spot but ≤97.64% to the T. thermophilus strains from geographically different places in Japan.

scholarly journals Genome Sequences of Two Choline-Utilizing Methanogenic Archaea, Methanococcoides spp., Isolated from Marine Sediments

2019 ◽  
Vol 8 (18) ◽  
Author(s):  
Gordon Webster ◽  
Alex J. Mullins ◽  
Andrew J. Watkins ◽  
Edward Cunningham-Oakes ◽  
Andrew J. Weightman ◽  
...  
Keyword(s):  
Marine Sediments ◽  
Dna Hybridization ◽  
In Silico ◽  
Methanogenic Archaea ◽  
Nucleotide Identity ◽  
Average Nucleotide Identity ◽  
Genome Sequences ◽  
Content Type

The genomes of two Methanococcoides spp. that were isolated from marine sediments and are capable of carrying out methanogenesis from choline and other methylotrophic substrates were sequenced. The average nucleotide identity and in silico DNA-DNA hybridization analyses demonstrate that they represent species different from those previously described.

scholarly journals Draft Genome Sequences of Plant-Pathogenic Klebsiella variicola Strains Isolated from Plantain in Haiti

2020 ◽  
Vol 9 (29) ◽  
Author(s):  
James C. Fulton ◽  
Jeannie M. Klein ◽  
Sladana Bec ◽  
Joubert Fayette ◽  
Karen A. Garrett ◽  
...  
Keyword(s):  
Type Strain ◽  
Draft Genome ◽  
Nucleotide Identity ◽  
Average Nucleotide Identity ◽  
Genome Sequences ◽  
Content Type

ABSTRACT The genus Klebsiella includes pathogenic and nonpathogenic species. We report the 5.57-Mb genome sequences of two Klebsiella variicola strains, G18-1365 and G18-1376, isolated from symptomatic plantain plants in Haiti. These strains are genetically closely related (average nucleotide identity [ANI] > 99%) to the previously described type strain of K. variicola, DSM 15968.

scholarly journals Re-identification of strains deposited as Pseudomonas aeruginosa, Pseudomonas fluorescens and Pseudomonas putida in GenBank based on whole genome sequences

2020 ◽  
Vol 70 (11) ◽  
pp. 5958-5963
Author(s):  
Yuh Morimoto ◽  
Mari Tohya ◽  
Zulipiya Aibibula ◽  
Tadashi Baba ◽  
Hiroyuki Daida ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Genome Sequencing ◽  
Dna Hybridization ◽  
Type Species ◽  
Whole Genome ◽  
Average Nucleotide Identity ◽  
Genome Sequences ◽  
Content Type ◽  
Link Type

The taxonomic classification of Pseudomonas species has been revised and updated several times. This study utilized average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) cutoff values of 95 and 70 %, respectively, to re-identify the species of strains deposited in GenBank as P. aeruginosa , P. fluorescens and P. putida . Of the 264 deposited P. aeruginosa strains, 259 were correctly identified as P. aeruginosa , but the remaining five were not. All 28 deposited P. fluorescens strains had been incorrectly identified as P. fluorescens . Four of these strains were re-identified, including two as P. kilonensis and one each as P. aeruginosa and P. brassicacearum , but the remaining 24 could not be re-identified. Similarly, all 35 deposited P. putida strains had been incorrectly identified as P. putida . Nineteen of these strains were re-identified, including 12 as P. alloputida , four as P. asiatica and one each as P. juntendi , P. monteilii and P. mosselii . These results strongly suggest that Pseudomonas bacteria should be identified using ANI and dDDH analyses based on whole genome sequencing when Pseudomonas species are initially deposited in GenBank/DDBJ/EMBL databases.

scholarly journals Genome Sequences of Five Streptomyces Bacteriophages Forming Cluster BG

2017 ◽  
Vol 5 (28) ◽  
Author(s):  
Richard Donegan-Quick ◽  
Zane A. Gibbs ◽  
Patricia O. Amaku ◽  
Joshua T. Bernal ◽  
Dana A. M. Boyd ◽  
...  
Keyword(s):  
Streptomyces Griseus ◽  
The Other ◽  
Nucleotide Identity ◽  
Strain Atcc ◽  
Average Nucleotide Identity ◽  
Genome Sequences ◽  
Content Type

ABSTRACT Cluster BG of the actinobacteriophage was formed upon discovery of five novel bacteriophages isolated by enrichment from their host, Streptomyces griseus subsp. griseus strain ATCC 10137. Four members of this cluster (BabyGotBac, Maih, TP1605, and YDN12) share over 89% average nucleotide identity, while the other (Xkcd426) has only 72% similarity to other cluster members.

Comparative genome analysis and phylogenomic of Xanthomonas citri pv. viticola lead to new taxonomic insights about species of Xanthomonas

2020 ◽  
Author(s):  
Antonio Roberto Gomes de Farias ◽  
Wilson José da Silva Junior ◽  
José Bandeira do Nascimento Junior ◽  
Valdir de Queiroz Balbino ◽  
Ana Maria Benko-Iseppon ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Dna Hybridization ◽  
In Silico ◽  
Whole Genome Sequence ◽  
Nucleotide Identity ◽  
Whole Genome ◽  
Average Nucleotide Identity ◽  
Xanthomonas Citri ◽  
Genome Sequences ◽  
Xanthomonas Species

Abstract Background Xanthomonas citri pv. viticola is one of the most critical grapevine diseases in the Northeast of Brazil, presenting a high risk to Brazilian and worldwide areas of grape production. The X.citri pv. viticola epithet was recently proposed to be changed from X. campestris pv. v iticola based on multilocus sequence analysis and whole-genome sequences. Besides, genomics has revolutionized the field of bacteriology, by associating genome sequencing with comparative analysis such as in silico analysis such as DNA-DNA hybridization, average nucleotide identity, distance between genomes, pan-genomic approach, and phylogenomic, providing valuable insights and knowledge about virulence factors and contributing to increase the understanding and clarifying the taxonomic relationship of Xanthomonas and others prokaryotic species.Results We used the whole-genome sequence of three Brazilian strains and the pathotype to characterize X.citri pv. viticola accessions plus 124 whole-genome sequences of Xanthomonas species available in NCBI, comprising 13 species and 15 pathovars. The whole-genome sequence structure of X. citri pv. viticola was shown presents a high level of conservation concerning other X. citri species. Pan-genomic approaches, average nucleotide identity analysis, and in silico DNA-DNA hybridization were carried out, allowing X.citri pv. viticola characterization and inferences on the phylogenetic relationships within Xanthomonas . The analysis of the sequence of the 128 genomes clustered the Xanthomonas strains in eight main groups according to the recently proposed classification in all approaches used. Also, the analysis revealed that X. hortorum and X. gardneri should be classified as a single species, and the strain 17 of X. campestris and XC01 of X. citri pv. mangiferaeindicae widely described in the literature are misclassified.Conclusions We performed the genomic characterization of three representative Brazilian strains of Xcv . The genomic approaches based in the pan-genome, average nucleotide identity, and in silico DNA-DNA hybridization support the proposed taxonomic position of X.citri pv. viticola and of the recently proposed Xanthomonas species and pathovars. In addition, we detected species delimitation of the misclassified Xanthomonas strains with extensive studies reported in the literature.

scholarly journals First Whole-Genome Characterization of Avian Nephritis Virus 2 of Broiler Chicken from Pará, Brazil

2018 ◽  
Vol 6 (25) ◽  
Author(s):  
Ceyla Maria Oeiras Castro ◽  
Elaine Hellen Nunes Chagas ◽  
Delana Andreza Melo Bezerra ◽  
Sandro Patroca da Silva ◽  
Ana Cecília Ribeiro Cruz ◽  
...  
Keyword(s):  
Broiler Chickens ◽  
Broiler Chicken ◽  
Whole Genome ◽  
Full Genome ◽  
Genome Sequences ◽  
Content Type ◽  
Genome Characterization ◽  
Avian Nephritis Virus ◽  
The Family

ABSTRACT Our results show the first full-genome characterization of avian nephritis virus 2 recovered from stools of broiler chickens at a commercial farm located in Benevides, Pará, Brazil. Nucleotide analyses of whole-genome sequences showed the isolate to be a strain of Avastrovirus 2 in the family Astroviridae.

scholarly journals Genome Sequence of the Alphaproteobacterium Blastochloris sulfoviridis DSM 729, Which Requires Reduced Sulfur as a Growth Supplement and Contains Bacteriochlorophyll b

2020 ◽  
Vol 9 (18) ◽  
Author(s):  
John A. Kyndt ◽  
Dayana Montano Salama ◽  
Terry E. Meyer
Keyword(s):  
Phylogenetic Analysis ◽  
Genome Sequence ◽  
Gc Content ◽  
Nucleotide Identity ◽  
Whole Genome ◽  
Average Nucleotide Identity ◽  
Content Type ◽  
Growth Supplement ◽  
Bacteriochlorophyll B ◽  
Reduced Sulfur

The genome sequence of Blastochloris sulfoviridis is 3.85 Mb with a GC content of 68%. Its nearest relative is B. tepida (average nucleotide identity [ANI], 91.5%), followed by B. viridis (ANI, 83%). According to ANI and whole-genome-based phylogenetic analysis, the nearest relatives of Blastochloris are Rhodoplanes and Rhodopseudomonas, confirming the recognition of distinct genera.

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