scholarly journals Coding-Complete Sequences of Recombinant Lumpy Skin Disease Viruses Collected in 2020 from Four Outbreaks in Northern Vietnam

2021 ◽  
Vol 10 (48) ◽  
Author(s):  
Elisabeth Mathijs ◽  
Frank Vandenbussche ◽  
Long Nguyen ◽  
Laetitia Aerts ◽  
Tho Nguyen ◽  
...  
Keyword(s):  
Disease Virus ◽  
Skin Disease ◽  
Lumpy Skin Disease ◽  
Lumpy Skin Disease Virus ◽  
Northern Vietnam ◽  
Complete Sequences ◽  
Important Disease

Lumpy skin disease virus (LSDV) causes a severe, systemic, and economically important disease in cattle. Here, we report coding-complete sequences of recombinant LSDVs from four outbreaks in October and November 2020 in northeastern Vietnam.

scholarly journals Complete Coding Sequence of a Lumpy Skin Disease Virus Strain Isolated during the 2016 Outbreak in Kazakhstan

2020 ◽  
Vol 9 (4) ◽  
Author(s):  
Elisabeth Mathijs ◽  
Frank Vandenbussche ◽  
Meruyert Saduakassova ◽  
Tursyn Kabduldanov ◽  
Andy Haegeman ◽  
...  
Keyword(s):  
Disease Virus ◽  
Virus Strain ◽  
Skin Disease ◽  
Clinical Sample ◽  
Lumpy Skin Disease ◽  
Lumpy Skin Disease Virus ◽  
Coding Sequence ◽  
Important Disease

Lumpy skin disease virus (LSDV) causes an economically important disease in cattle. Here, we report the complete coding sequence of the LSDV isolate Kubash/KAZ/16, detected in a clinical sample from an infected cow from the outbreak reported on 7 July 2016 in Kazakhstan (Atyrau Region).

scholarly journals Complete Genome Sequence of the Lumpy Skin Disease Virus Isolated from the First Reported Case in Greece in 2015

2017 ◽  
Vol 5 (29) ◽  
Author(s):  
Eirini I. Agianniotaki ◽  
Elisabeth Mathijs ◽  
Frank Vandenbussche ◽  
Konstantia E. Tasioudi ◽  
Andy Haegeman ◽  
...  
Keyword(s):  
Disease Virus ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Skin Disease ◽  
Complete Genome ◽  
Lumpy Skin Disease ◽  
Lumpy Skin Disease Virus ◽  
Important Disease

ABSTRACT Lumpy skin disease virus (LSDV) causes an economically important disease in cattle. Here, we report the complete genome sequence of the first LSDV isolate identified in mainland Europe. LSDV isolate Evros/GR/15 was isolated from the first cases reported on 18 August 2015 in the Evros region, Greece.

scholarly journals Complete Genome Sequences of the Neethling-Like Lumpy Skin Disease Virus Strains Obtained Directly from Three Commercial Live Attenuated Vaccines

2016 ◽  
Vol 4 (6) ◽  
Author(s):  
Elisabeth Mathijs ◽  
Frank Vandenbussche ◽  
Andy Haegeman ◽  
Alasdair King ◽  
Bethuel Nthangeni ◽  
...  
Keyword(s):  
Disease Virus ◽  
Skin Disease ◽  
Complete Genome ◽  
Animal Health ◽  
Genome Sequences ◽  
Lumpy Skin Disease ◽  
Lumpy Skin Disease Virus ◽  
Live Attenuated Vaccines ◽  
Virus Strains ◽  
Important Disease

Lumpy skin disease virus (LSDV) causes an economically important disease in cattle. Here, we report the complete genome sequences of three LSDV strains obtained directly from the live attenuated vaccines: Lumpyvax (MSD Animal Health), Herbivac LS (Deltamune) and Lumpy Skin Disease Vaccine (Onderstepoort Biological Products).

scholarly journals Comparative Evaluation of Lumpy Skin Disease Virus-Based Live Attenuated Vaccines

Vaccines ◽  
2021 ◽  
Vol 9 (5) ◽  
pp. 473
Author(s):  
Andy Haegeman ◽  
Ilse De Leeuw ◽  
Laurent Mostin ◽  
Willem Van Campe ◽  
Laetitia Aerts ◽  
...  
Keyword(s):  
Disease Virus ◽  
Skin Disease ◽  
Vaccination Campaign ◽  
Ease Of Use ◽  
Vaccine Failure ◽  
Lumpy Skin Disease ◽  
Lumpy Skin Disease Virus ◽  
The Balkans ◽  
The Past ◽  
Preventative Strategy

Vaccines form the cornerstone of any control, eradication and preventative strategy and this is no different for lumpy skin disease. However, the usefulness of a vaccine is determined by a multiplicity of factors which include stability, efficiency, safety and ease of use, to name a few. Although the vaccination campaign in the Balkans against lumpy skin disease virus (LSDV) was successful and has been implemented with success in the past in other countries, data of vaccine failure have also been reported. It was therefore the purpose of this study to compare five homologous live attenuated LSDV vaccines (LSDV LAV) in a standardized setting. All five LSDV LAVs studied were able to protect against a challenge with virulent LSDV. Aside from small differences in serological responses, important differences were seen in side effects such as a local reaction and a Neethling response upon vaccination between the analyzed vaccines. These observations can have important implications in the applicability in the field for some of these LSDV LAVs.

scholarly journals Production of small ruminant morbillivirus, rift valley fever virus and lumpy skin disease virus in CelCradle™ -500A bioreactors

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Halima Rhazi ◽  
Najete Safini ◽  
Karima Mikou ◽  
Meryeme Alhyane ◽  
Khalid Omari Tadlaoui ◽  
...  
Keyword(s):  
Disease Virus ◽  
Skin Disease ◽  
Rift Valley ◽  
Rift Valley Fever ◽  
Rift Valley Fever Virus ◽  
Fever Virus ◽  
Valley Fever ◽  
Lumpy Skin Disease ◽  
Lumpy Skin Disease Virus ◽  
Cell Factories

Abstract Background Animal vaccination is an important way to stop the spread of diseases causing immense damage to livestock and economic losses and the potential transmission to humans. Therefore effective method for vaccine production using simple and inexpensive bioprocessing solutions is very essential. Conventional culture systems currently in use, tend to be uneconomic in terms of labor and time involved. Besides, they offer a limited surface area for growth of cells. In this study, the CelCradle™-500A was evaluated as an alternative to replace conventional culture systems in use such as Cell factories for the production of viral vaccines against small ruminant morbillivirus (PPR), rift valley fever virus (RVF) and lumpy skin disease virus (LSD). Results Two types of cells Vero and primary Lamb Testis cells were used to produce these viruses. The study was done in 2 phases as a) optimization of cell growth and b) virus cultivation. Vero cells could be grown to significantly higher cell densities of 3.04 × 109 using the CelCradle™-500A with a shorter doubling time as compared to 9.45 × 108 cells in Cell factories. This represents a 19 fold increase in cell numbers as compared to seeding vs only 3.7 fold in Cell factories. LT cells achieved modestly higher cell densities of 6.7 × 108 as compared to 6.3 × 108 in Cell factories. The fold change in densities for these cells was 3 fold in the CelCradle™-500A vs 2.5 fold in Cell factories. The titers in the conventional system and the bioreactor were not significantly different. However, the Cell-specific virus yield for rift valley fever virus and lumpy skin disease virus are higher (25 virions/cell for rift valley fever virus, and 21.9 virions/cell for lumpy skin disease virus versus 19.9 virions/cell for rift valley fever virus and 10 virions/cell for lumpy skin disease virus). Conclusions This work represents a novel study for primary lamb testis cell culture in CellCradle™-500A bioreactors. In addition, on account of the high cell densities obtained and the linear scalability the titers could be further optimized using other culture process such us perfusion.

Molecular characterization of lumpy skin disease virus in Iran (2014–2018)

2021 ◽  
Author(s):  
Zeinab Hedayati ◽  
Hamid Reza Varshovi ◽  
Ali Mohammadi ◽  
Mohammad Tabatabaei
Keyword(s):  
Molecular Characterization ◽  
Disease Virus ◽  
Skin Disease ◽  
Lumpy Skin Disease ◽  
Lumpy Skin Disease Virus

scholarly journals Cutaneous lymphoma in a cow - a case report

2019 ◽  
Vol 73 (1) ◽  
pp. 50-56
Author(s):  
Branislav Kureljusic ◽  
Slobodan Maksimovic ◽  
Slobodan Vujinovic ◽  
Bozidar Savic ◽  
Vesna Milicevic ◽  
...  
Keyword(s):  
Disease Virus ◽  
Skin Disease ◽  
Mononuclear Cells ◽  
Subcutaneous Tissue ◽  
Leukemia Virus ◽  
Surgical Excision ◽  
Cutaneous Lymphoma ◽  
Histopathological Analysis ◽  
Lumpy Skin Disease ◽  
Lumpy Skin Disease Virus

In this paper the case of a 2.5-year-old Simmental cow, with suspicion of lumpy skin disease in mid-2017 in Serbia will be presented. Clinical examination revealed numerous nodules of varied size from a few millimeters to approximately 10 centimeters disseminated predominantly on the skin of the udder and the perineum, some of which were coalescing and exulcerated. The general condition of the affected animal was unchanged. According to the results of laboratory analysis, the cow was negative for the presence of antibodies against the bovine leukemia virus, showed a negative reaction in tuberculinization and was vaccinated against lumpy skin disease virus. After the surgical excision of one skin node, the sample tested negative for the genome of lumpy skin disease virus. In order to establish the morphology of the skin lesion, a histopathological analysis was performed. Histopathological analysis showed the infiltration of the corium and subcutaneous tissue by numerous mononuclear cells showing cellular atypia. Suspicion of cutaneous lymphoma was established. Furthermore, the immunohistochemical examination confirmed that the infiltrate contained exclusively CD3-immunopositive cells, suggesting a T-cell origin nonepitheliotropic lymphoma.

scholarly journals Retention of Lumpy Skin Disease Virus in Stomoxys Spp (Stomoxys Calsitrans, Stomoxys Sitiens, Stomoxys Indica) following intrathoracic inoculation, Diptera: Muscidae

2020 ◽  
Author(s):  
Arman Issimov ◽  
Lespek Kutumbetov ◽  
Assylbek Zhanabayev ◽  
Nurlybay Kazhgaliyev ◽  
Birzhan Nurgaliyev ◽  
...  
Keyword(s):  
Disease Virus ◽  
Skin Disease ◽  
Virus Isolation ◽  
Emerging Disease ◽  
Lumpy Skin Disease ◽  
Lumpy Skin Disease Virus ◽  
Laboratory Conditions ◽  
Means Of Transmission ◽  
Intrathoracic Inoculation

AbstractLumpy skin disease (LSD) is an emerging disease in cattle in Kazakhstan and the means of transmission remains uncertain. In the current study, acquisition of Lumpy Skin Disease Virus (LSDV) by Stomoxys species following intrathoracic inoculation was demonstrated under laboratory conditions. Flies were injected with a virulent LSDV strain into the thorax region to bypass the midgut barrier. The fate of pathogen in the hemolymph of the flies was further examined using PCR and Virus isolation tests. LSDV was isolated from all three Stomoxys species immediately and up to 24h post intrathoracic inoculation while virus DNA was detectable up to 7d post intrathoracic inoculation.

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