Effect of Yeast Extract on h6h Gene Expression and Tropane Alkaloids Production in Atropa belladonna L. Hairy Roots

2021 ◽  
Vol 68 (1) ◽  
pp. 102-109
Author(s):  
A. Hedayati ◽  
S. Hemmaty ◽  
E. Nourozi ◽  
A. Amirsadeghi
Keyword(s):  
Gene Expression ◽  
Hairy Roots ◽  
Yeast Extract ◽  
Tropane Alkaloids ◽  
Atropa Belladonna

CRISPR/Cas9-mediated disruption of the PYRROLIDINE KETIDE SYNTHASE gene reduces the accumulation of tropane alkaloids in Atropa belladonna hairy roots

2021 ◽  
Author(s):  
Fumihito Hasebe ◽  
Honoka Yuba ◽  
Takashi Hashimoto ◽  
Kazuki Saito ◽  
Nobutaka Funa ◽  
...  
Keyword(s):  
Hairy Roots ◽  
Biosynthetic Pathway ◽  
Polyketide Synthase ◽  
Tropane Alkaloids ◽  
Tropane Alkaloid ◽  
Atropa Belladonna ◽  
Type Iii Polyketide Synthase ◽  
Canonical Type ◽  
Solanaceae Family

Abstract Tropane alkaloids, including clinically important hyoscyamine and scopolamine, are produced in the roots of medicinal plant species, such as Atropa belladonna, from the Solanaceae family. Recent molecular and genomic approaches have advanced our understanding of the metabolic enzymes involved in tropane alkaloid biosynthesis. A non-canonical type III polyketide synthase (PKS), pyrrolidine ketide synthase (PYKS), catalyzes a two-step decarboxylative reaction, which involves imine-ketide condensation indispensable to tropane skeleton construction. In this study, we generated pyks mutant A. belladonna hairy roots via CRISPR/Cas9-mediated genome editing and analyzed the metabolic consequences of the loss of PYKS activity on tropane alkaloids, providing insights into a crucial role of the scaffold-forming reaction in the biosynthetic pathway.

Differential Production of Tropane Alkaloids in Hairy Roots and in vitro Cultured Two Accessions of Atropa belladonna L. under Nitrate Treatments

2010 ◽  
Vol 65 (5-6) ◽  
pp. 373-379 ◽  
Author(s):  
Najmeh Ahmadian Chashmi ◽  
Mozafar Sharifi ◽  
Farah Karimi ◽  
Hasan Rahnama
Keyword(s):  
Secondary Metabolites ◽  
Hairy Roots ◽  
Nitrate Concentration ◽  
Tropane Alkaloids ◽  
Atropa Belladonna ◽  
Remarkable Effect ◽  
Aerial Parts ◽  
Potential Source ◽  
Stable Production

Plants are a potential source of a large number of valuable secondary metabolites. In vitro cultures are being considered as an alternative to agricultural processes for studying valuable secondary metabolites. In this way, nutritive factors are important parameters influencing the production of these compounds in plants. Effects of nitrate concentrations (KNO3) on the production of two tropane alkaloids, hyoscyamine and scopolamine, and the growth of aerial parts and roots of two in vitro propagated accessions of Atropa belladonna and hairy roots were investigated. As hairy roots cultures are able to keep a stable production of alkaloids over long periods of subculturing, they are considered as an interesting option for the study of alkaloid biosynthesis. A hairy roots culture of Atropa belladonna was established by transformation with Agrobacterium rhizogenes strain AR15834. The results of our study showed that a rise in KNO3 concentration caused a decline in hairy roots growth, and had a remarkable effect on the alkaloid content. The alkaloid concentrations obtained in the hairy roots were 3 - 20 times higher than that in the plants at 35 mM of KNO3. Increasing the nitrate concentration in the medium of hairy roots also improved the hyoscyamine/scopolamine ratio, while it increased the scopolamine/hyoscyamine ratio in the studied plants.

Effects of Elicitors on Tropane Alkaloids and Gene Expression inAtropa baeticaTransgenic Hairy Roots

2008 ◽  
Vol 71 (12) ◽  
pp. 2026-2031 ◽  
Author(s):  
Nabil el Jaber-Vazdekis ◽  
Marie Laure Barres ◽  
Ángel G. Ravelo ◽  
Rafael Zárate
Keyword(s):  
Gene Expression ◽  
Hairy Roots ◽  
Tropane Alkaloids

Investigation of Tropane Alkaloids in Genetically Transformed Atropa belladonna L. Cultures

2004 ◽  
Author(s):  
H. Hank ◽  
�. Sz?ke ◽  
K. T�th ◽  
I. L�szl� ◽  
L. Kursinszki
Keyword(s):  
Tropane Alkaloids ◽  
Atropa Belladonna

scholarly journals Enhanced secretion of tropane alkaloids in Nicotiana tabacum hairy roots expressing heterologous hyoscyamine-6β-hydroxylase

2005 ◽  
Vol 56 (420) ◽  
pp. 2611-2618 ◽  
Author(s):  
Suvi T. Häkkinen ◽  
Elisabeth Moyano ◽  
Rosa M. Cusidó ◽  
Javier Palazón ◽  
M. Teresa Piñol ◽  
...  
Keyword(s):  
Nicotiana Tabacum ◽  
Hairy Roots ◽  
Tropane Alkaloids ◽  
Enhanced Secretion
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