Investigation of Tropane Alkaloids in Genetically Transformed Atropa belladonna L. Cultures

2004 ◽  
Author(s):  
H. Hank ◽  
�. Sz?ke ◽  
K. T�th ◽  
I. L�szl� ◽  
L. Kursinszki
Keyword(s):  
Tropane Alkaloids ◽  
Atropa Belladonna

CRISPR/Cas9-mediated disruption of the PYRROLIDINE KETIDE SYNTHASE gene reduces the accumulation of tropane alkaloids in Atropa belladonna hairy roots

2021 ◽  
Author(s):  
Fumihito Hasebe ◽  
Honoka Yuba ◽  
Takashi Hashimoto ◽  
Kazuki Saito ◽  
Nobutaka Funa ◽  
...  
Keyword(s):  
Hairy Roots ◽  
Biosynthetic Pathway ◽  
Polyketide Synthase ◽  
Tropane Alkaloids ◽  
Tropane Alkaloid ◽  
Atropa Belladonna ◽  
Type Iii Polyketide Synthase ◽  
Canonical Type ◽  
Solanaceae Family

Abstract Tropane alkaloids, including clinically important hyoscyamine and scopolamine, are produced in the roots of medicinal plant species, such as Atropa belladonna, from the Solanaceae family. Recent molecular and genomic approaches have advanced our understanding of the metabolic enzymes involved in tropane alkaloid biosynthesis. A non-canonical type III polyketide synthase (PKS), pyrrolidine ketide synthase (PYKS), catalyzes a two-step decarboxylative reaction, which involves imine-ketide condensation indispensable to tropane skeleton construction. In this study, we generated pyks mutant A. belladonna hairy roots via CRISPR/Cas9-mediated genome editing and analyzed the metabolic consequences of the loss of PYKS activity on tropane alkaloids, providing insights into a crucial role of the scaffold-forming reaction in the biosynthetic pathway.

Feeding experiments using suspension cultures of Atropa belladonna; limiting steps in the biosynthesis of tropane alkaloids

1990 ◽  
Vol 29 (11) ◽  
pp. 3517-3523 ◽  
Author(s):  
Liisa Kaarina Simola ◽  
Riitta Parviainen ◽  
Aarne Martinsen ◽  
Aarre Huhtikangas ◽  
Reija Jokelat ◽  
...  
Keyword(s):  
Tropane Alkaloids ◽  
Suspension Cultures ◽  
Atropa Belladonna ◽  
Feeding Experiments

TROPANE ALKALOIDS PRODUCTION FROM CALLUS CULTURE OF ATROPA BELLADONNA L. AS AFFECTED BY ELICITORS AND PRECURSOR FEEDING

2018 ◽  
Vol 9 (7) ◽  
pp. 116-125
Author(s):  
Mohamed I Elshorbagy ◽  
Souzan M Ibrahim ◽  
Kamilia A Abo El-Seoud ◽  
Ahmed I Abd El-Maksoud
Keyword(s):  
Callus Culture ◽  
Tropane Alkaloids ◽  
Atropa Belladonna ◽  
Precursor Feeding

Differential Production of Tropane Alkaloids in Hairy Roots and in vitro Cultured Two Accessions of Atropa belladonna L. under Nitrate Treatments

2010 ◽  
Vol 65 (5-6) ◽  
pp. 373-379 ◽  
Author(s):  
Najmeh Ahmadian Chashmi ◽  
Mozafar Sharifi ◽  
Farah Karimi ◽  
Hasan Rahnama
Keyword(s):  
Secondary Metabolites ◽  
Hairy Roots ◽  
Nitrate Concentration ◽  
Tropane Alkaloids ◽  
Atropa Belladonna ◽  
Remarkable Effect ◽  
Aerial Parts ◽  
Potential Source ◽  
Stable Production

Plants are a potential source of a large number of valuable secondary metabolites. In vitro cultures are being considered as an alternative to agricultural processes for studying valuable secondary metabolites. In this way, nutritive factors are important parameters influencing the production of these compounds in plants. Effects of nitrate concentrations (KNO3) on the production of two tropane alkaloids, hyoscyamine and scopolamine, and the growth of aerial parts and roots of two in vitro propagated accessions of Atropa belladonna and hairy roots were investigated. As hairy roots cultures are able to keep a stable production of alkaloids over long periods of subculturing, they are considered as an interesting option for the study of alkaloid biosynthesis. A hairy roots culture of Atropa belladonna was established by transformation with Agrobacterium rhizogenes strain AR15834. The results of our study showed that a rise in KNO3 concentration caused a decline in hairy roots growth, and had a remarkable effect on the alkaloid content. The alkaloid concentrations obtained in the hairy roots were 3 - 20 times higher than that in the plants at 35 mM of KNO3. Increasing the nitrate concentration in the medium of hairy roots also improved the hyoscyamine/scopolamine ratio, while it increased the scopolamine/hyoscyamine ratio in the studied plants.

scholarly journals Development of Atropa belladonna L. Plants with High-Yield Hyoscyamine and without Its Derivatives Using the CRISPR/Cas9 System

2021 ◽  
Vol 22 (4) ◽  
pp. 1731
Author(s):  
Lingjiang Zeng ◽  
Qiaozhuo Zhang ◽  
Chunxue Jiang ◽  
Yueyue Zheng ◽  
Youwei Zuo ◽  
...  
Keyword(s):  
Low Cost ◽  
Tropane Alkaloids ◽  
High Yield ◽  
In Planta ◽  
Atropa Belladonna ◽  
Potential Applicability ◽  
Herbal Plants ◽  
New Varieties ◽  
High Yields ◽  
First Time

Atropa belladonna L. is one of the most important herbal plants that produces hyoscyamine or atropine, and it also produces anisodamine and scopolamine. However, the in planta hyoscyamine content is very low, and it is difficult and expensive to independently separate hyoscyamine from the tropane alkaloids in A. belladonna. Therefore, it is vital to develop A. belladonna plants with high yields of hyoscyamine, and without anisodamine and scopolamine. In this study, we generated A. belladonna plants without anisodamine and scopolamine, via the CRISPR/Cas9-based disruption of hyoscyamine 6β-hydroxylase (AbH6H), for the first time. Hyoscyamine production was significantly elevated, while neither anisodamine nor scopolamine were produced, in the A. belladonna plants with homozygous mutations in AbH6H. In summary, new varieties of A. belladonna with high yields of hyoscyamine and without anisodamine and scopolamine have great potential applicability in producing hyoscyamine at a low cost.

Sign in / Sign up

Export Citation Format

Share Document