Genetic Differentiation of the Wolf Canis lupus L. Populations from Siberia at Microsatellite Loci

2020 ◽  
Vol 56 (1) ◽  
pp. 59-68 ◽  
Author(s):  
M. S. Talala ◽  
A. Ya. Bondarev ◽  
E. S. Zakharov ◽  
D. V. Politov
Keyword(s):  
Genetic Differentiation ◽  
Canis Lupus ◽  
Microsatellite Loci

Genetic Differentiation of the Round Goby (Neogobius melanostomus) from Certain Localities of the Black and Azov Sea Basin Using Microsatellite Loci

2021 ◽  
Vol 55 (3) ◽  
pp. 236-247
Author(s):  
S. I. Tarasjuk ◽  
V. V. Zamorov ◽  
O. V. Zaloilo ◽  
O. Iu. Bielikova ◽  
D. B. Radionov
Keyword(s):  
Genetic Differentiation ◽  
Microsatellite Loci ◽  
Round Goby ◽  
Neogobius Melanostomus ◽  
Azov Sea

scholarly journals MICROSATELLITE POLYMORPHISM WITHIN TWO LAKE BAIKAL OILFISH SPECIES (COMEPHORUS LACEPEDE, 1801)

2007 ◽  
Vol 5 (2) ◽  
pp. 50-57 ◽  
Author(s):  
Veronika I Teterina ◽  
Lubov V Sukhanova ◽  
Sergey V Kirilchik
Keyword(s):  
Genetic Polymorphism ◽  
Genetic Differentiation ◽  
Lake Baikal ◽  
Microsatellite Loci ◽  
Passive Transfer ◽  
Microsatellite Analysis ◽  
Microsatellite Polymorphism ◽  
Baikal Lake ◽  
Water Currents ◽  
Cottus Gobio

Intraspecific genetic polymorphism of a Baikal Lake endemic, big Baikal oilfish (Comephorus baicalensis Pallas, 1776), was evaluated based on microsatellite analysis. The obtained results have compared to the results received earlier for a little Baikal oilfish (C. dybowski Korotneff, 1905). Six microsatellite loci designed for the European sculpin, Cottus gobio, were used. Big Baikal oilfish samples were tested from the three Baikal trenches (southern, middle, northern). Average values of F<sub>ST</sub> and R<sub>ST</sub>indexes between the samples did not exceed 0,02 that point to weak intraspecific genetic differentiation. Big Baikal oilfish had the smaller allele variety, the greater deficit of heterozygotes and presence of low-molecular alleles in comparison with little Baikal oilfish. The values of genetic differentiation were equaled F<sub>ST</sub> = 0,138 and R<sub>ST</sub> = 0,244 for all loci between cumulative samples of little and big Baikal oilfish. Analysis of microsatellite polymorphism has showed that both a big and little Baikal oilfishes are represented by single populations. The factor promoting panmixia within the Baikal oilfish species is apparently a passive transfer of juvenile and adult fishes by water currents.

Genetic Differentiation of Rhododendron aureum Georgi at Nuclear Microsatellite Loci

2019 ◽  
Vol 55 (6) ◽  
pp. 762-766
Author(s):  
M. A. Polezhaeva ◽  
N. A. Tikhonova ◽  
M. V. Modorov ◽  
M. N. Ranyuk ◽  
O. S. Korchagina
Keyword(s):  
Genetic Differentiation ◽  
Microsatellite Loci ◽  
Nuclear Microsatellite ◽  
Rhododendron Aureum ◽  
Rhododendron Aureum Georgi

Genetic variation within and relationships among five subpopulations of Slovak Thoroughbred

2004 ◽  
Vol 52 (3) ◽  
pp. 259-265
Author(s):  
Daniela Šátková-Jakabová ◽  
J. Trandžík ◽  
Ľudmila Hudecová-Kvasňáková ◽  
Erika Hegedüšová-Zetochová ◽  
A. Bugarský ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Genetic Differentiation ◽  
International Society ◽  
Microsatellite Loci ◽  
Genetic Relationships ◽  
Allele Frequencies ◽  
Weinberg Equilibrium ◽  
Hardy Weinberg Equilibrium

Genetic variation at six microsatellite loci was analysed for five Thoroughbred subpopulations to determine the magnitude of genetic differentiation and the genetic relationships among the subpopulations. Significant deviations from Hardy-Weinberg equilibrium were shown for a number of locus-population combinations, with all subpopulations. The genetic diversities and relationships of five Thoroughbred subpopulations were evaluated using six microsatellites recommended by the International Society of Animal Genetics (ISAG). The allele frequencies, the effective numbers of alleles, and the observed and expected heterozygosities were calculated. POPGENE v. 1.31 (Yeh et al., 1997) was used to test for deviations from the Hardy-Weinberg (H-W) equilibrium and to assign FIS estimates (Weir, 1990). The utility of microsatellites for evaluating genetic diversity of horses is discussed.

scholarly journals Microsatellite Polymorphism and Genetic Structure of Florida Mottled Duck Populations

The Condor ◽  
2002 ◽  
Vol 104 (2) ◽  
pp. 424-431 ◽  
Author(s):  
Christen L. Williams ◽  
Richard C. Brust ◽  
Olin E. Rhodes Jr
Keyword(s):  
Genetic Structure ◽  
Genetic Differentiation ◽  
Habitat Loss ◽  
Microsatellite Loci ◽  
Nuclear Dna ◽  
Gene Frequencies ◽  
Limited Distribution ◽  
Mottled Duck ◽  
Anas Fulvigula ◽  
High Level

Abstract Little is understood concerning the effects regional isolation and habitat loss have had upon the genetic structure of Mottled Ducks (Anas fulvigula), a nonmigratory waterfowl species with a limited distribution. Our objective was to identify nuclear DNA-based markers for Mottled Ducks and determine levels of subdivision among populations in Florida. We screened 13 microsatellite primer pairs and identified six microsatellite loci that were variable in Mottled Ducks. These markers revealed a low level of genetic differentiation and a high level of genetic exchange among four Mottled Duck subpopulations within Florida. Over all populations, single-locus expected heterozygosities over the six loci surveyed ranged from 0.13–0.85. There were no significant differences in gene frequencies among the populations examined, and the Fst over 5 biparental loci was not different from zero. Our analysis of the Florida Mottled Duck population indicated high levels of heterozygosity and no evidence of genetic subdivision among breeding units. Polimorfismo en Microsatélites y Estructura Genética en Poblaciones de Anas fulvigula Resumen. Se tiene escaso conocimiento acerca de los efectos del aislamiento regional y de la pérdida de hábitat sobre la estructura genética de poblaciones de Anas fulvigula, un ave acuática no migratoria de distribución restringida. Nuestro objetivo fue identificar marcadores genéticos de ADN para A. fulvigula y determinar los niveles de subdivisión entre poblaciones en Florida. Examinamos 13 pares de iniciadores (i.e., primers) para microsatélites e identificamos seis loci que fueron variables en A. fulvigula. Estos marcadores revelaron un bajo nivel de diferenciación genética y un alto grado de intercambio genético entre cuatro subpoblaciones de A. fulvigula en Florida. Para todas las poblaciones, la heterocigocidad esperada en un locus varió entre 0.13–0.85 para los seis loci examinados. No hubo diferencias significativas en las frecuencias génicas entre las subpoblaciones examinadas, y el valor de Fst para los 5 loci biparentales no fue diferente de cero. Nuestros análisis de las poblaciones de A. fulvigula de Florida indicaron altos niveles de heterocigocidad y no mostraron evidencia de subdivisión genética entre las unidades reproductivas.

scholarly journals Tracing back the origin of pumpkins ( Cucurbita pepo ssp. pepo L.) in Mexico

2019 ◽  
Vol 286 (1908) ◽  
pp. 20191440 ◽  
Author(s):  
Gabriela Castellanos-Morales ◽  
Karen Y. Ruiz-Mondragón ◽  
Helena S. Hernández-Rosales ◽  
Guillermo Sánchez-de la Vega ◽  
Niza Gámez ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Genetic Differentiation ◽  
Microsatellite Loci ◽  
Approximate Bayesian Computation ◽  
Cucurbita Pepo ◽  
Improved Varieties ◽  
Nuclear Microsatellite ◽  
Well Differentiated ◽  
Approximate Bayesian ◽  
Chloroplast Haplotypes

Cucurbita pepo is an economically important crop, which consists of cultivated C. pepo ssp. pepo , and two wild taxa ( C. pepo ssp. fraterna and C. pepo ssp. ovifera ) . We aimed at understanding the domestication and the diversity of C. pepo in Mexico. We used two chloroplast regions and nine nuclear microsatellite loci to assess the levels of genetic variation and structure for C. pepo ssp. pepo 's landraces sampled in 13 locations in Mexico, five improved varieties, one C. pepo ssp. fraterna population and ornamental C. pepo ssp. ovifera . We tested four hypotheses regarding the origin of C. pepo ssp. pepo 's ancestor through approximate Bayesian computation: C. pepo ssp. ovifera as the ancestor; C. pepo ssp. fraterna as the ancestor; an unknown extinct lineage as the ancestor; and C. pepo ssp. pepo as hybrid from C. pepo ssp. ovifera and C. pepo ssp. fraterna ancestors. Cucurbita pepo ssp. pepo showed high genetic variation and low genetic differentiation. Cucurbita pepo ssp. fraterna and C. pepo ssp. pepo shared two chloroplast haplotypes. The three subspecies were well differentiated for microsatellite loci. Cucurbita pepo ssp. fraterna was probably C. pepo ssp. pepo 's wild ancestor, but subsequent hybridization between taxa complicate defining C. pepo ssp. pepo 's ancestor.

High nuclear genetic differentiation, but low chloroplast diversity in a rare species, Aluta quadrata (Myrtaceae), with a disjunct distribution in the Pilbara, Western Australia

2016 ◽  
Vol 64 (8) ◽  
pp. 687 ◽  
Author(s):  
M. Byrne ◽  
D. J. Coates ◽  
B. M. Macdonald ◽  
M. Hankinson ◽  
S. M. McArthur ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Gene Flow ◽  
Genetic Structure ◽  
Genetic Differentiation ◽  
Microsatellite Loci ◽  
Disjunct Distribution ◽  
Chloroplast Gene ◽  
Seed Sources ◽  
Chloroplast Haplotype ◽  
Haplotype Variation

Geographically separated populations may show high levels of genetic differentiation, depending on the levels of current and historical isolation. In the ancient landscape of the Pilbara region, there are few plant species with restricted distributions, and one such species, Aluta quadrata Rye & Trudgen, is restricted to three separate locations on the southern edge of the Hamersley Range. We investigated genetic diversity and differentiation among geographically isolated locations of A. quadrata, using 10 microsatellite loci to assess contemporary genetic structure, and sequences of seven chloroplast gene regions to infer historical isolation. Nuclear genetic diversity was moderate, with moderate to high genetic differentiation among the three locations, and low differentiation among populations within locations. In contrast, there was no detected variation in the chloroplast genome. The high genetic differentiation is consistent with limited contemporary connectivity among the geographically separated locations, although lack of chloroplast haplotype variation indicates that limited connectivity has occurred more recently and is not due to historical isolation. The level of differentiation suggests use of local seed sources for augmentation or establishment of populations within gene flow distance of existing populations, whereas an experimental translocation established on more distant sites could use mixed seed sources to maximise genetic diversity.

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